Chemical composition and anticancer effects of Hyptis Suaveolens L. Poit (lamiaceae) volatile oil

2021 ◽  
Vol 25 (1) ◽  
pp. 64-69
Author(s):  
R.O. Imade ◽  
B.A. Ayinde ◽  
M.I. Choudhary ◽  
A. Alam
Keyword(s):  
Brine Shrimp ◽  
Volatile Oil ◽  
Artemia Salina ◽  
Cytotoxic Effects ◽  
Anticancer Effects ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity ◽  
Hyptis Suaveolens ◽  
Diphenyltetrazolium Bromide ◽  
Type Apparatus

The leaf of Hyptis suaveolens have found application in ethnomedicine in the treatment of various ailments including those that are related to tumor and cancer. This study was therefore undertaken to evaluate the cytotoxic effects of its volatile oil. Volatile oil distilled from freshly collected leaves using a Clavenger-type apparatus was screened using tadpoles of Raniceps ranninus (10-40 µg/mL) and brine shrimp of Artemia salina (10-1000 µg/mL) with bench-top assay procedures for cytotoxicity while growth inhibitory activity was assessed using radicles of Sorghum bicolor seeds (1-30 mg/mL). The essential oils were further tested on breast cancer (AU 565) and cervical cancer (HeLa) at 50 µg /mL using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay and afterwards subjected to Gas Chromatography Mass Spectrometric (GCMS) analysis for its constituents. An LC50 of 188.67 and 8 µg/mL were obtained in the brine shrimp mortality and tadpole lethality assays respectively. The oil showed inhibitions of 86.74 and 21.8 % against AU 565 and HeLa cells respectively. GCMS analysis revealed the major constituents as sabinene (10.64 %) and (-)-4-terpineol (7.27 %). These results support its use in treating tumor-related ailments and should be considered for further studies.

Chemical composition and anticancer effects of Zingiber officinale volatile oil

2021 ◽  
Vol 25 (1) ◽  
pp. 60-63
Author(s):  
R.O. Imade ◽  
B.A. Ayinde ◽  
M.I. Choudhary ◽  
A. Alam
Keyword(s):  
Brine Shrimp ◽  
Zingiber Officinale ◽  
Volatile Oil ◽  
Cytotoxicity Test ◽  
Distillation Method ◽  
Anticancer Activities ◽  
Plant Oil ◽  
Cytotoxic Effects ◽  
Preliminary Screening ◽  
Anticancer Effects

Zingiber officinale rhizome is used in ethnomedicine in treating tumor-related ailments. This study was undertaken to evaluate the cytotoxic effects of this plant. The oil was extracted using a Clavenger apparatus by hydro-distillation method. Preliminary screening was carried out with brine shrimp cytotoxicity test at 10-1000 µg/mL. The volatile oil was further tested on breast cancer (AU 565) and cervical cancer (HeLa) at 50 µg /mL using MTT assay and later subjected to GCMS analysis. LC50 of 157.75 µg/mL was obtained in the brine shrimp mortality assay. Z. officinale oil showed high anticancer activities with 50 and 43 % inhibitions against HeLa and AU 565 cells respectively. GCMS analysis revealed the major constituents of Z. officinale oil as a-citral (11.68 %) and a-citral (10.18 %). These results suggest the medicinal potency of this plant oil.

scholarly journals In vivo and in vitro antiplasmodial activities of some plants traditionally used in Guatemala against malaria.

1997 ◽  
Vol 41 (7) ◽  
pp. 1500-1503 ◽  
Author(s):  
F F Franssen ◽  
L J Smeijsters ◽  
I Berger ◽  
B E Medinilla Aldana
Keyword(s):  
Plasmodium Berghei ◽  
Brine Shrimp ◽  
Folk Medicine ◽  
Artemia Salina ◽  
In Vitro Cultures ◽  
Cytotoxic Effects ◽  
Resistant Strains ◽  
Simarouba Glauca

We present an evaluation of the antiplasmodial and cytotoxic effects of four plants commonly used in Guatemalan folk medicine against malaria. Methanol extracts of Simarouba glauca D. C., Sansevieria guineensis Willd, Croton guatemalensis Lotsy, and Neurolaena lobata (L.)R.Br. significantly reduced parasitemias in Plasmodium berghei-infected mice. Dichloromethane fractions were screened for their cytotoxicities on Artemia salina (brine shrimp) larvae, and 50% inhibitory concentrations were determined for Plasmodium falciparum in in vitro cultures. Both chloroquine-susceptible and -resistant strains of P. falciparum were significantly inhibited by these extracts. Of all dichloromethane extracts, only the S. glauca cortex extract was considered to be toxic to nauplii of A. salina in the brine shrimp test.

scholarly journals Cytotoxic Activity of Inositol Angelates and Tirucallane-Type Alkaloids from Amoora Dasyclada

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1222
Author(s):  
Sheng-Xiang Yang ◽  
Cheng-Gang Song ◽  
Yi Kuang ◽  
Bing Liu ◽  
Yan-Xin Zhang ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Cytotoxic Activity ◽  
Human Cancer ◽  
Cancer Cell Line ◽  
Artemia Salina ◽  
Human Cancer Cell ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity ◽  
Ic50 Value

Three new inositol angelate compounds (1–3) and two new tirucallane-type alkaloids (4 and 5) were isolated from the Amoora dasyclada, and their structures were established mainly by means of combination of 1D and 2D nuclear magnetic resonance and HR-ESI-MS. Based on cytotoxicity testing, compounds 4 and 5 exhibited significant cytotoxic activity against human cancer cell line HepG2 with IC50 value at 8.4 and 13.2 μM. In addition, compounds 4 and 5 also showed remarkable growth inhibitory activity to Artemia salina larvae.

scholarly journals Cytotoxic Metabolites Produced by Alternaria No.28, an Endophytic Fungus Isolated from Ginkgo biloba

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Jian-Chun Qin ◽  
Ya-Mei Zhang ◽  
Ling Hu ◽  
Ya-Tuan Ma ◽  
Jin-Ming Gao
Keyword(s):  
Ginkgo Biloba ◽  
Endophytic Fungus ◽  
Brine Shrimp ◽  
Culture Media ◽  
Monomethyl Ether ◽  
Artemia Salina ◽  
Fungal Endophyte ◽  
Alternariol Monomethyl Ether ◽  
Cytotoxic Effects

From the medicinal plant Ginkgo biloba the fungal endophyte Alternaria no.28 was isolated. Extract of the fungus grown in liquid culture media exhibited marked cytotoxic activity when tested in vitro against brine shrimp (Artemia salina). Eight compounds were isolated from the extract of cultures of this endophytic fungus and were elucidated as alterperylenol (1), altertoxin I (2), alternariol (3), alternariol monomethyl ether (4), tenuazonic acid (5) and its derivative (6), together with ergosterol and ergosta-4, 6, 8, 22-tetraen-3-one by means of spectroscopic analysis. Among them, both 5 and 6 showed significant cytotoxic effects in the brine shrimp bioassy, with mortality rates of 73.6% and 68.9%, respectively, at a concentration of 10 μg.mL−1, and they were first isolated from endophytic fungi.

scholarly journals Phytochemical and Biological Investigations of Ixora arborea Roxb.

1970 ◽  
Vol 8 (2) ◽  
pp. 161-166 ◽  
Author(s):  
Fahima Aktar ◽  
Md Abul Kaisar ◽  
ANM Hamidul Kabir ◽  
Choudhury M Hasan ◽  
Mohammad A Rashid
Keyword(s):  
Petroleum Ether ◽  
Brine Shrimp ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Spectroscopic Studies ◽  
Total Phenolic ◽  
Brine Shrimp Lethality ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity ◽  
Ic50 Value

A total of four compounds namely betulin (1), erythrodiol (2), lupeol (3), and stigmasterol were isolated from the leaf extract of Ixora arborea for the first time. The structures of the isolated compounds were established by extensive spectroscopic studies. In our preliminary screening, the petroleum ether, carbon tetrachloride, dichloromethane and aqueous soluble fractions of the methanol extract were subjected to antioxidant, antimicrobial and brine shrimp lethality bioassays. All of the fractions showed significant antioxidant activity along with the total phenolic content, of which the methanolic extract of leaves (MEL) showed the highest free radical scavenging activity with IC50 value 33.0 μg/ml. In the brine shrimp lethality bioassay, among all the extractives the petroleum ether soluble fraction (PESF) demonstrated significant cytotoxicity with LC50 value of 0.622 μg/ml. However, in case of antimicrobial screening, only the methanolic crude extract showed mild growth inhibitory activity against the tested microorganisms. Key words: Ixora arborea; betulin; erythrodiol; lupeol; stigmasterol; antioxidant; cytotoxicity; antimicrobial DOI: 10.3329/dujps.v8i2.6031 Dhaka Univ. J. Pharm. Sci. 8(2): 161-166, 2009 (December)

Antibacterial and Cytotoxic Effects of the Cultivated Ruta chalepensis

2020 ◽  
Vol 16 (5) ◽  
pp. 654-660
Author(s):  
Lynda Gali ◽  
Fatiha Bedjou
Keyword(s):  
Antibacterial Activity ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Volatile Oil ◽  
Diffusion Method ◽  
Chloroform Fraction ◽  
Bacterial Strains ◽  
Cytotoxic Effects ◽  
Ruta Chalepensis ◽  
Low Concentrations

Background: Ruta chalepensis is a shrub from the Mediterranean basin widely used in the traditional medicine. The plant presents an interesting composition containing alkaloids, coumarins and volatile oil. The present work aims to study the antibacterial and the cytotoxic effects of the ethanol extract, its fractions, and the alkaloid extract from the cultivated Ruta chalepensis. Methods: Extracts were tested against five bacterial strains using the agar well diffusion method and the broth micro-dilution technique for the determination of the Minimum Inhibitory Concentration (MIC). Brine shrimp assay was used to evaluate the cytotoxicity. Results: Chloroform fraction exhibited a strong antibacterial activity against S. aureus, and B. subtilis with inhibition diameters of 25.5±0.7 and 18.5±2.12mm at 50mg/ml and 100mg/ml, respectively. Butanol and aqueous fractions were found to be inactive against all the tested strains. Ethyl acetate was the only active fraction against E. coli. Alkaloids showed a strong growth inhibition of B. subtilis and S. aureus at low concentrations (22.5±0.71 and 18.00±0,00mm, respectively at 20mg/ml). The cytotoxicity evaluation using the brine shrimp larvae indicated a high effect of alkaloids with an LC50 of 27.51μg/ml supporting their antibacterial activity. Conclusion: These findings suggest a possible use of Ruta chalepensis as a source of antibacterial and anti-proliferative agents.

scholarly journals Phytochemical and Biological Investigations of Ixora lutea Hutch.

1970 ◽  
Vol 8 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Shapna Sultana ◽  
Mohammad S Rahman ◽  
Md Aslam Hossain ◽  
Md Khalid Hossain ◽  
Mohammad A Rashid
Keyword(s):  
Antioxidant Activity ◽  
Petroleum Ether ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Preliminary Screening ◽  
Brine Shrimp Lethality ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity ◽  
Ic50 Value

Two triterpenoids, lupeol (1) and stigmasterol were isolated from the petroleum ether soluble fractionof the methanolic extract of Ixora lutea Hutch. In our preliminary screening, the petroleum ether, carbontetrachloride, chloroform soluble fractions of methanol extract of the leaves and stem were subjected to antioxidant,antimicrobial and brine shrimp lethality bioassay. All of the fractions showed moderate to potent antioxidant activity,of which the chloroform soluble fraction demonstrated the strongest antioxidant activity with the IC50 value of 3.0μg/ml. In case of antimicrobial screening, all extractives showed mild growth inhibitory activity. However, in thebrine shrimp lethality bioassay, the petroleum ether soluble fraction was found to be most cytotoxic among thepartitionates having LC50 value 0.938 μg/ml.Key words: Ixora lutea; rubiaceae; lupeol; stigmasterol; antioxidant activity; brine shrimp lethality bioassay;antimicrobial.DOI: 10.3329/dujps.v8i1.5331Dhaka Univ. J. Pharm. Sci. 8(1): 17-21, 2009 (June)

scholarly journals The Thrombolytic and Cytotoxic Effects of Nigella sativa (L.) Seeds: The Prophetic Medicine

2020 ◽  
Vol 2 (2) ◽  
pp. 70-77
Author(s):  
Zahir Uddin Mohammed Babar ◽  
Irwandi Jaswir ◽  
Mohd Hafidz Mahamad Maifiah ◽  
Soraya Ismail ◽  
Raha Ahmad Raus ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Nigella Sativa ◽  
Blood Clot ◽  
Artemia Salina ◽  
Water Soluble ◽  
Clot Lysis ◽  
Dependent Manner ◽  
Cytotoxic Effects ◽  
Thrombolytic Effect ◽  
Dose Dependent

The Water-Soluble Extract (WSE) is a crude bioactive phytoconstituent of Nigella sativa (L.) seeds discovered recently. The current findings report about the thrombolytic and cytotoxic effects of WSE using human blood clot lysis and brine shrimp lethality (BSL) bioassay. The thrombolytic effect of WSE (1,666.67 µg/mL) was determined via the clot and lysate weight measurements compared to streptokinase (STK) of 30,000 IU/mL and normal saline (NS) while the cytotoxicity of WSE (44.14-2,000 µg/mL) against vincristine sulfate (VCS;3.125-100 µg/mL). WSE has shown extremely statistically significant (p<0.0001) clot lysis (90.00%) compared to NS (3.76%) whilst it was also significantly different (p<0.0063) to STK (72.41%) exhibiting LC50 of 1,795.90 µg/mL vs. VCS (39.25 µg/mL) in a dose-dependent manner. The current results suggested WSE has a potent thrombolytic effect with mild dose-dependent cytotoxicity towards brine shrimp nauplii (Artemia salina). It also suggested WSE might have enzymatic roles on thrombin, fibrin, and plasmin of blood. This pharmacological action of WSE is might be due to its antioxidant property, short-chain fatty acids and/or amino acids. Further studies are highly recommended on the enzymatic role(s) and bioactive phytoconstituents of WSE.

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