Post-chemotherapy miR-146a expression and its prognostic potential in oral cancer patients

Purpose: To determine miR-146a expression level after chemotherapy in oral cancer patients, and itsprognostic value.Methods: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used for the determination of miR-146 expression levels. Based on the results, the analysis of the miR-146a expression in oral cancer patients was performed by drawing ROC curve to provide information on the prognostic value of miR-146a. The survival of the patients was monitored over a period of 5 years. The patients were categorized into high- and low-expression groups, and multivariate Cox regression analysis method was adopted to provide a more comprehensive analysis of individual risk factors influencing the prognosis of oral cancer.Results: The miR-146a expression level in patients after chemotherapy was lower than that in patients before they received chemotherapy (p < 0.05). The specificity of using miR-146a to predict oral cancer was 76.83 %, the sensitivity 69.44 %, and the area between the curve and x-axis 0.78. In contrast, the survival level was significantly greater in high-expression patients (p < 0.05).Conclusion: The independent risk parameters for buccal carcinoma are drinking, smoking, chronic leukoplakia, and miR-146a.

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Relationship of Common Variants in miR-27a-3p Gene with Susceptibility and Prognosis of Oral Cancer

10.21203/rs.3.rs-57383/v1 ◽

2020 ◽

Author(s):

Lili Wang ◽

Hongguang Song ◽

Shiming Yang

Keyword(s):

Regression Analysis ◽

Oral Cancer ◽

Cancer Patients ◽

Prognostic Value ◽

Cox Regression ◽

Poor Overall Survival ◽

Cox Regression Analysis ◽

Kaplan Meier ◽

Oral Cancer Patients ◽

Cancer Tissues

Abstract Background: miR-27a-3p has been found dysexpressed in various cancers. The aim of the present study was to clarify the prognostic value of miR-27a-3p in patients with oral cancer.Methods: We used quantitative real-time polymerase chain reaction (qRT-PCR) assay to detect the expression of miR-27a-3p in the tissue of oral cancer and adjacent normal specimens. The association of miR-27a-3p with clinicopathological characteristics was analyzed via the Chi-square test. Kaplan-Meier survival and Cox regression analysis were performed to evaluate the prognostic value of miR-27a-3p in oral cancer patients.Results: The down-regulated expression of miR-27a-3p was found in oral cancer tissues compared with the matched noncancerous samples (P<0.05). And its expression was influenced by TNM stage (P=0.032), T stage (P=0.014) and lymph node metastasis (P=0.025). Kaplan–Meier analysis result showed that the decreased level of miR-27a-3p expression was associated with a poor overall survival of oral cancer patients. Additionally, multivariate cox regression analysis revealed that the low expression of miR-27a-3p was an independent prognostic maker in oral cancer patients (HR=0.462, 95% CI=0.223-0.957, P=0.038).Conclusions: Taken together, the expression pattern of miR-27a-3p was decreased in oral cancer tissues. The decreased expression of miR-27a-3p was a potential prognostic biomarker in patients with oral cancer.

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Adjuvant Radiotherapy Significantly Increases Neck Control and Survival in Early Oral Cancer Patients with Solitary Nodal Involvement: A National Cancer Registry Database Analysis

Cancers ◽

10.3390/cancers13153742 ◽

2021 ◽

Vol 13 (15) ◽

pp. 3742

Author(s):

Chia-Jen Tsai ◽

Yu-Hsuan Kuo ◽

Hung-Chang Wu ◽

Chung-Han Ho ◽

Yi-Chen Chen ◽

...

Keyword(s):

Oral Cancer ◽

Cancer Patients ◽

Cancer Registry ◽

Adjuvant Radiotherapy ◽

Multivariable Analysis ◽

Nodal Involvement ◽

Cox Regression Analysis ◽

Oral Cancer Patients ◽

Neck Control ◽

Registry Database

We assessed the role of adjuvant radiotherapy on neck control and survival in patients with early oral cancer with solitary nodal involvement. We identified pT1-2N1 oral cancer patients with or without adjuvant radiotherapy from the 2007–2015 Taiwan Cancer Registry database. The effect of adjuvant radiotherapy on 5-year neck control, overall survival (OS) and disease-free survival (DFS) were calculated using the Kaplan–Meier method, log-rank tests, and Cox regression analysis. Of 701 patients identified, 505 (72.0%) received adjuvant radiotherapy and 196 (28.0%) had surgery alone. Patients receiving adjuvant radiotherapy were more likely to be aged <65 years, pT2 stage, poorly graded and without comorbid conditions (all, p < 0.05). The 5-year OS and DFS differed significantly by receipt of adjuvant radiotherapy. Multivariable analysis showed adjuvant radiotherapy significantly associated with better 5-year OS (adjusted hazard ratio (aHR), 0.72; 95% confidence interval (CI), 0.54–0.97; p = 0.0288) and DFS (aHR, 0.64; 95% CI, 0.48–0.84; p = 0.0016). Stratified analysis indicated the greatest survival advantage for both 5-year OS and DFS in those with pT2 classification (p = 0.0097; 0.0009), and non-tongue disease (p = 0.0195; 0.0158). Moreover, adjuvant radiotherapy significantly protected against neck recurrence (aHR, 0.30; 95% CI, 0.18–0.51; p < 0.0001). Thus, adjuvant radiotherapy is associated with improved neck control and survival in these early oral cancer patients.

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Risk Group Definition in Children with Acute Myeloid Leukemia by Calculating Individual Risk Factors on the Basis of a Multivariate Stepwise Cox Regression Analysis

Leukemia & Lymphoma ◽

10.1080/10428190127506 ◽

2001 ◽

Vol 42 (6) ◽

pp. 1289-1295 ◽

Cited By ~ 1

Author(s):

Daniel Steinbach ◽

Johann Hermann ◽

Tim Littlewood ◽

Felix Zintl

Keyword(s):

Risk Factors ◽

Acute Myeloid Leukemia ◽

Regression Analysis ◽

Myeloid Leukemia ◽

Cox Regression ◽

Risk Group ◽

Individual Risk ◽

Cox Regression Analysis ◽

Individual Risk Factors ◽

Acute Myeloid

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Risk Group Definition in Children with Acute Myeloid Leukemia by Calculating Individual Risk Factors on the Basis of a Multivariate Stepwise Cox Regression Analysis

Leukemia & Lymphoma ◽

10.3109/10428190109097754 ◽

2001 ◽

Vol 42 (6) ◽

pp. 1289-1295 ◽

Cited By ~ 1

Author(s):

Daniel Steinbach ◽

Johann Hermann ◽

Tim Littlewood ◽

Felix Zintl

Keyword(s):

Risk Factors ◽

Acute Myeloid Leukemia ◽

Regression Analysis ◽

Myeloid Leukemia ◽

Cox Regression ◽

Risk Group ◽

Individual Risk ◽

Cox Regression Analysis ◽

Individual Risk Factors ◽

Acute Myeloid

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The association of miR-138 variants with the prognosis of cervical cancer

10.21203/rs.3.rs-50657/v1 ◽

2020 ◽

Author(s):

Shuangqing Cao ◽

Lei Zheng

Keyword(s):

Cervical Cancer ◽

Overall Survival ◽

Cancer Patients ◽

Cox Regression ◽

Critical Role ◽

Expression Level ◽

Poor Overall Survival ◽

Cox Regression Analysis ◽

Normal Tissues ◽

Kaplan Meier

Abstract Background MicroRNA-138 (miR-138) is shown to inhibit tumor growth and played a critical role in tumor pathogenesis, the present study aimed to investigate the prognistic value of miR-138 in cervical cancer. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) assay was used to detect the expression of miR-138 in the tissues of cervical cancer and adjacent normal tissues. The association of miR-138 expression with clinical characteristic was analyzed via χ2 test. Then Kaplan-Meier analysis was performed to analyze the association of miR-138 expression with the overall survival of cervical cancer patients. The multivariate cox analysis was used to evaluate the prognostic value of miR-138. Results In the current study, we found the expression level of miR-138 was significantly downregulated in the most cervical cancer patients tissues compared with that in the adjacent normal tissues (P < 0.001). And its expression was closely affected by TNM stage (P = 0.043), lymph node metastasis (P = 0.011) and FIGO stage (P = 0.002). Kaplan-Meier analysis result showed that the decreased expression level of miR-138 expression was associated with poor overall survival of patients. The cox regression analysis result indicated that miR-138 expression was independently associated with the overall survival. Conclusions The expression of miR-138 is down-regulated and involved in the development of cervical cancer. Moreover, it may serve as a prognostic marker for patients with cervical cancer.

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NKX6.1 Expression is Associated With The Prognosis in Breast Cancer.

10.21203/rs.3.rs-47888/v1 ◽

2020 ◽

Author(s):

Jie Zhang ◽

Sujie Zhang ◽

Xiaoyan Li ◽

Fan Zhang ◽

Lei Zhao

Keyword(s):

Breast Cancer ◽

Overall Survival ◽

Cancer Patients ◽

Cox Regression ◽

Expression Level ◽

Rank Test ◽

Breast Cancer Patients ◽

Cox Regression Analysis ◽

Log Rank Test ◽

Cancer Tissues

Abstract Background: Breast cancer is the most common cancer among women in the world. NKX6.1 is proved to be involved in several human cancers, but fewer researches have reported the functional roles of NKX6.1 in breast cancer. In this study, we investigated the clinical significance of NKX6.1 expression in breast cancer prognosis.Methods: The expression level of NKX6.1 in breast cancer tissues and paired non-cancerous tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Chi-square test was applied to evaluate the relationship between NKX6.1 expression and clinicopathologic parameters. The overall survival of breast cancer patients were analyzed by Kaplan-Meier method with log rank test. Additionally, cox regression analysis was used for prognosis analysis.Results: NKX6.1 expression level is increased in breast cancer tissues (P<0.001). Moreover, the elevated levels were significantly correlated with tumor size (P=0.002), TNM stage (P=0.018) and lymph node metastasis (P=0.007). In addition, breast cancer patients with high NKX6.1 level had a poorer overall survival than those with low level (log rank test, P=0.001). NKX6.1 was an independent prognostic factor for breast cancer (HR=2.961, 95%CI=1.368-6.411, P=0.006).Conclusions: NKX6.1 is up-regulated in breast cancer, which may be a potential prognostic biomarker for the cancer.

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