A Simple Medium for Isolation of Coagulase-Positive Staphylococci in a Single Step

1982 ◽  
Vol 45 (3) ◽  
pp. 218-222 ◽  
Author(s):  
LEONIE MINTZER-MORGENSTERN ◽  
ELIY AHU KATZENELSON
Keyword(s):  
Agar Medium ◽  
Brain Heart Infusion ◽  
Egg Yolk ◽  
Food Samples ◽  
Selective Medium ◽  
Single Step ◽  
Isolation And Identification ◽  
Complete Reversal ◽  
Simple Medium ◽  
Coagulase Positive Staphylococci

An agar medium containing NaCl, egg yolk and tellurite for selective quantitative isolation of coagulase-positive staphylococci from food was developed. Isolation and identification of the staphylococci was achieved in a single step. A properly diluted food sample was spread over the medium and incubated for 24 h at 42 C. Coagulase-positive staphylococci appeared as small grey to dark-grey colonies surrounded by a dense white opacity. Coagulase-negative bacteria which, at times, grow on this medium, did not produce this reaction. The identification on this selective medium of isolates from 683 different food samples as coagulase-positive staphylococci was subsequently confirmed by the coagulase test. Comparative titrations of 29 various coagulase-positive staphylococcus strains on both the selective medium and nutrient agar yielded nearly identical titers. The growth of heat-stressed staphylococci was inhibited by the selective medium. Complete reversal of the inhibition was achieved by a 3-h pre-incubation in brain heart infusion at 37 C.

Un milieu gélosé pour le dénombrement direct de Staphylococcus aureus : milieu au plasma de porc pour S. aureus (PPSA)

1976 ◽  
Vol 22 (11) ◽  
pp. 1603-1611 ◽  
Author(s):  
J-J. Devoyod ◽  
Liliane Millet ◽  
G. Mocquot
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Products ◽  
Agar Medium ◽  
Egg Yolk ◽  
Plasma Medium ◽  
Large Numbers ◽  
Selective Agar ◽  
Coagulase Positive Staphylococci

A selective agar medium (pork plasma medium for S. aureus (PPSA)) enables the direct enumeration of coagulase-positive staphylococci. This medium is based on the Baird-Parker agar without egg yolk and is supplemented with pig plasma. Colonies of Staphylococcus aureus are surrounded by a halo of precipitated fibrin. When foods such as dairy products contain large numbers of egg yolk – negative strains of S. aureus, the PPSA agar has the advantage over egg yolk containing media such as Baird-Parker agar that fewer suspect colonies have to be confirmed.

scholarly journals Isolation and Identification of Listeria Monocytogenes in Bangalore City from Various Food Samples

2021 ◽  
Vol 14 (4) ◽  
pp. 2271-2276
Author(s):  
Vedavati Goudar ◽  
Kanthesh B M ◽  
Nagalambika Prasad
Keyword(s):  
Listeria Monocytogenes ◽  
Ice Cream ◽  
Raw Milk ◽  
Food Samples ◽  
Selective Medium ◽  
Chicken Meat ◽  
Isolation And Identification ◽  
Fresh Milk ◽  
Biochemical Identification ◽  
Raw Fish

The current research emphasis on the isolation and differentiation of Listeria monocytogenes from different food samples most frequently infected with Listeriosis outbreaks. Crude chicken meat, raw milk, pasteurized cheese, ice cream and raw fish are samples from the city of Bangalore. The selective medium mainly used for the isolation of Listeria is oxford agar. Using isolated L. monocytogenes from food samples, morphologic and biochemical identification was carried out. 2 samples (fresh milk and Ice cream) were positive out of 5 samples; 3 samples (raw chicken meat, raw fish, and pasteurized cheese) were negative. The results conferred during this study indicate the contamination of Ice- cream and Raw Milk samples with L. monocytogenes.

An improved selective and diagnostic medium for the isolation and enumeration of Bacillus cereus in foods

1980 ◽  
Vol 26 (7) ◽  
pp. 753-759 ◽  
Author(s):  
R. Holbrook ◽  
Judith M. Anderson
Keyword(s):  
Bacillus Cereus ◽  
Egg Yolk ◽  
Bromothymol Blue ◽  
Food Samples ◽  
Selective Medium ◽  
Confirmatory Test ◽  
Staining Procedure ◽  
Complete Agreement ◽  
Phenol Red ◽  
Biochemical Tests

The use and performance of an improved diagnostic and selective medium, PEMBA (polymyxin pyruvate egg yolk mannitol bromothymol blue agar), for the detection of Bacillus cereus in foods is described. The distinct colonial appearance of B. cereus on PEMBA permitted the recognition of both strains: those that do precipitate egg yolk and those that do not react with egg yolk. A staining procedure, used to demonstrate microscopically both the presence of lipid globules in vegetative cells and spore morphology of isolates, proved a rapid and reliable confirmatory test which gave complete agreement with a battery of biochemical tests used for this purpose. The quantitative recovery of B. cereus on PEMBA from 143 food samples was not significantly different from counts on KG (Kim and Goepfert), MYP (mannitol egg yolk phenol red), and McClung's media, and the selectivity of PEMBA was generally superior.

scholarly journals Study on the isolation and identification of Pasteurella species from farm animal and human

2008 ◽  
Vol 2 (1) ◽  
pp. 81-90
Author(s):  
Sawsan salman Atia mubarak ◽  
Ismail khatham Shubar ◽  
Amar salim Rahiem
Keyword(s):  
Pasteurella Multocida ◽  
Agar Medium ◽  
Brain Heart Infusion ◽  
Farm Animal ◽  
Brain Heart Infusion Broth ◽  
Laboratory Animals ◽  
Isolation And Identification ◽  
Selective Agar

This study was interested in isolation and identification of Pasteuralla species in animals and human , also studing it's sensitivity to antibiotic with comparison to it's pathogenicity for laboratory animals . Tow groups of samples were collected and investigeted, 1st group consisting of one handered thirty six samples which were collected from animals ( cow .sheep , goats , and chikens ) . 2nd group was also consisting of 136 samples that were colleeted from human ( wound , urine , and sputum ) . These samples were cultured in selective enrichement brain heart infusion broth and then in the new Pasteurella multocida selective agar medium .

scholarly journals Development of an Improved Selective Agar Medium for Isolation of Yersinia pestis

2003 ◽  
Vol 69 (10) ◽  
pp. 5787-5792 ◽  
Author(s):  
Raphael Ber ◽  
Emanuelle Mamroud ◽  
Moshe Aftalion ◽  
Avital Tidhar ◽  
David Gur ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Quantitative Evaluation ◽  
Fluorescent Protein ◽  
Agar Medium ◽  
Brain Heart Infusion ◽  
Selective Medium ◽  
Bacterial Populations ◽  
Medium Components ◽  
Selective Agar ◽  
Green Fluorescent

ABSTRACT Existing media designed for selective isolation of clinically important members of the genus Yersinia were found to be unsatisfactory for the growth and isolation of Yersinia pestis. We report the development of a new selective agar medium (termed BIN) that supports the growth of Y. pestis. The development of the formulation of this medium was based on a fluorescence screening system designed for monitoring bacterial growth on semisolid media, using a green fluorescent protein-expressing strain. High-throughput combinatorial experiments can be conducted for the quantitative evaluation of the effect of different medium components on growth. Generation of fluorescence plots in this system, using microplates, allowed the quantitative evaluation of the growth rate of Y. pestis EV76 cultures in different agar compositions. The final BIN formulation is based on brain heart infusion agar, to which the selective agents irgasan, cholate salts, crystal violet, and nystatin were introduced. It was found that BIN agar is more efficient in supporting colony formation and recovery of Y. pestis than are the conventional semisolid media MacConkey agar and Yersinia-selective agar (cefsulodin-irgasan-novobiocin agar). The advantage of BIN over other media has been also demonstrated in recovering virulent Y. pestis from the mixed bacterial populations found in decaying carcasses of infected mice. The BIN medium is suggested as a selective medium for isolation and recovery of Y. pestis from various backgrounds.

THE PREDOMINANT BACTERIA IN NATURAL ZOOGLOEAL COLONIES: I. ISOLATION AND IDENTIFICATION

1967 ◽  
Vol 13 (12) ◽  
pp. 1671-1682 ◽  
Author(s):  
Richard F. Unz ◽  
Norman C. Dondero
Keyword(s):  
Waste Water ◽  
Single Cell ◽  
Agar Medium ◽  
Isolation And Identification ◽  
Group I ◽  
Yeast Autolysate ◽  
Conventional Methods ◽  
Group Ii ◽  
Produce Acid

Direct, single-cell isolations of bacteria, primarily from natural, branching, waste water zoogloeas, were made by micromanipulation. Isolations were also made by conventional methods. Direct isolates were classified, chiefly with regard to zoogloea formation, into two groups designated group I (zoogloea-forming) and group II (nonzoogloea-forming). Casitone – glycerol – yeast autolysate agar medium was best for the isolation of group I bacteria. Group I isolates reduced nitrate to gas, possessed urease and catalase, and gave positive oxidase reactions. They were generally able to hydrolyze gelatin but, with one exception, did not produce acid from carbohydrates, and none produced H2S, indole, or acetylmethylcarbinol or utilized Koser citrate. Group II strains were usually more diverse on differential tests and could be distinguished from group I strains. Group I strains were characterized as Zoogloea strains and were found to be the predominant bacteria in natural, branching, zoogloeal colonies.

scholarly journals Improvement of the Culture Medium for the Dichlorvos-Ammonia (DV-AM) Method to Selectively Detect Aflatoxigenic Fungi in Soil

Toxins ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 519 ◽  
Author(s):  
Kimiko Yabe ◽  
Haruna Ozaki ◽  
Takuya Maruyama ◽  
Keisuke Hayashi ◽  
Yuki Matto ◽  
...  
Keyword(s):  
Culture Medium ◽  
Agar Medium ◽  
Carbon Sources ◽  
Tween 80 ◽  
Selective Medium ◽  
Selective Detection ◽  
Aflatoxigenic Fungi ◽  
Aspergillus Nomius ◽  
Rhizopus Sp ◽  
Triton X

The dichlorvos-ammonia (DV-AM) method is a simple but sensitive visual method for detecting aflatoxigenic fungi. Here we sought to develop a selective medium that is appropriate for the growth of aflatoxigenic fungi among soil mycoflora. We examined the effects of different concentrations of carbon sources (sucrose and glucose) and detergents (deoxycholate (DOC), Triton X-100, and Tween 80) on microorganisms in soils, using agar medium supplemented with chloramphenicol. The results demonstrated that 5–10% sucrose concentrations and 0.1–0.15% DOC concentrations were appropriate for the selective detection of aflatoxigenic fungi in soil. We also identified the optimal constituents of the medium on which the normal rapid growth of Rhizopus sp. was completely inhibited. By using the new medium along with the DV-AM method, we succeeded in the isolation of aflatoxigenic fungi from non-agricultural fields in Fukui city, Japan. The fungi were identified as Aspergillus nomius based on their calmodulin gene sequences. These results indicate that the new medium will be useful in practice for the detection of aflatoxigenic fungi in soil samples including those from non-agricultural environments.

scholarly journals TSST-1, enterotoxin and bacteriocin-like substance production by Staphylococcus aureus isolated from foods

2013 ◽  
Vol 65 (5) ◽  
pp. 1537-1544 ◽  
Author(s):  
S.A. Carvalho ◽  
L.S. Carmo ◽  
E.F. Abreu ◽  
R.S. Dias ◽  
A.C.M. Apolônio ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bovine Milk ◽  
Food Poisoning ◽  
Brain Heart Infusion ◽  
Food Samples ◽  
Antagonistic Activity ◽  
Toxic Shock ◽  
Toxic Shock Syndrome Toxin ◽  
Reference Strains ◽  
Infusion Agar

The production of Toxic Shock Syndrome Toxin-1 (TSST-1), enterotoxins and bacteriocin-like substances was evaluated in 95 strains of Staphylococcus aureus recovered from raw bovine milk (n=31) and from food samples involved in staphylococcal food poisoning (n=64). Enterotoxigenicity tests with the membrane over agar associated to optimal sensibility plate assays were performed and showed that 96.77% of strains recovered from milk and 95.31% from food samples produced enterotoxins A, B, C, D or TSST-1. Reference strains S. epidermidis, Bacillus cereus, Listeria monocytogenes, Lactobacillus casei, Pseudomonas aeruginosa, S. aureus, Salmonella Typhimurium, Escherichia coli, Enterococcus faecalis and Bacteroides fragilis were used as indicator bacteria in the antagonistic assays, the first five being sensitive to antagonistic substances. Brain heart infusion agar, in pH values ranging from 5.0 to 7.0 in aerobic atmosphere showed to be the optimum condition for antagonistic activity as evaluated with the best producer strains against the most sensitive indicator bacterium, L. monocytogenes. Sensitivity to enzymes confirmed the proteinaceous nature of these substances. Neither bacteriophage activity nor fatty acids were detected and the antagonistic activity was not due to residual chloroform. Results did not establish a positive correlation between the bacteriocinogenic profile and toxigenicity in the tested S. aureus strains.

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