Study of Adhesion of Lactobacillus casei CRL 431 to Ileal Intestinal Cells of Mice

It is well known that the cell wall of Lactobacillus casei CRL 431, a strain present in probiotics, presents lectinlike surface molecules. Presence of these molecules stimulates the immune system. Given the role that lectins and lectinlike substances play in the adhesion phenomenon, it is probable that this is an initial stage in the immunostimulation produced by this bacterium. To confirm this, adhesion of this microorganism to exfoliated mouse ileal epithelial cells was studied in vitro. Other L. casei strains isolated from adult human intestines and one of dairy origin were also examined for their ability to adhere to ileal epithelial cells. Another strain, which was included in the present study, was Lactobacillus acidophilus CRL 730. L. casei strains isolated from humans showed good ability to adhere to ileal epithelial cells, whereas L. casei isolated from dairy origin did not. Adhesion was only observed at 37°C and at a pH between 6 and 7.5. The exposure time needed for highest adhesion was 30 min. Presence of lectinlike substances on the surface of L. casei CRL 431 is important to this adhesion phenomenon, since adherence capacity was lost after removal of these substances.

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Effect of a Mixture of Lactobacillus casei and Lactobacillus acidophilus Administered Orally on the Immune System in Mice

Journal of Food Protection ◽

10.4315/0362-028x-49.12.986 ◽

1986 ◽

Vol 49 (12) ◽

pp. 986-989 ◽

Cited By ~ 46

Author(s):

GABRIELA PERDIGON ◽

MARIA ELENA NADER de MACIAS ◽

SUSANA ALVAREZ ◽

MARTA MEDICI ◽

GUILLERMO OLIVER ◽

...

Keyword(s):

Immune System ◽

Lactobacillus Acidophilus ◽

Lactobacillus Casei ◽

Lymphocyte Activation ◽

Fermented Milk ◽

Peritoneal Macrophages ◽

Oral Route ◽

Carbon Clearance ◽

Stimulation Of

The effects of an orally-administered mixture of Lactobacillus casei and Lactobacillus acidophilus on the immune system in Swiss albino mice were studied. Non-fermented milk containing viable cultures of both microorganisms was fed for different consecutive days to the animals, the effect of such feeding on their immune system was evinced by macrophage and lymphocyte activation. An increase both in the in vitro phagocytic activity of peritoneal macrophages and in the carbon clearance activity was observed. As regard the lymphocytic activity, the mixture produced a higher activation than that in the control mice. The enhanced macrophage and lymphocytic activity by administering cultures via the oral route, suggest the advisability of using the mixture of bacteria for a more efficient stimulation of the host immune response.

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Proteomic response of inflammatory stimulated intestinal epithelial cells to in vitro digested plums and cabbages rich in carotenoids and polyphenols

Food & Function ◽

10.1039/c6fo00674d ◽

2016 ◽

Vol 7 (10) ◽

pp. 4388-4399 ◽

Cited By ~ 4

Author(s):

Anouk Kaulmann ◽

Sébastien Planchon ◽

Jenny Renaut ◽

Yves-Jacques Schneider ◽

Lucien Hoffmann ◽

...

Keyword(s):

Epithelial Cells ◽

Inflammatory Bowel Diseases ◽

Intestinal Epithelial Cells ◽

Intestinal Cells ◽

Intestinal Epithelial ◽

Bowel Diseases ◽

Inflammatory Bowel ◽

Proteomic Response

Proteomic response of intestinal cells as a model of inflammatory bowel diseases to digested plum and cabbage rich in polyphenols and carotenoids.

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Effects of different kind of fructans on in vitro growth of Lactobacillus acidophilus, Lactobacillus casei and Bifidobacterium lactis

African Journal of Microbiology Research ◽

10.5897/ajmr11.455 ◽

2011 ◽

Vol 5 (21) ◽

Author(s):

Juan Antonio Rendón-Huerta

Keyword(s):

Lactobacillus Acidophilus ◽

Lactobacillus Casei ◽

In Vitro Growth ◽

Bifidobacterium Lactis

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Calycosin Suppresses Epithelial Derived Initiative Key Factors and Maintains Epithelial Barrier in Allergic Inflammation via TLR4 Mediated NF-κB Pathway

Cellular Physiology and Biochemistry ◽

10.1159/000485416 ◽

2017 ◽

Vol 44 (3) ◽

pp. 1106-1119 ◽

Cited By ~ 13

Author(s):

Yu Tao ◽

Yan Wang ◽

Xiaoyu Wang ◽

Can Wang ◽

Kaifang Bao ◽

...

Keyword(s):

Epithelial Cells ◽

Western Blot ◽

Chinese Herb ◽

Allergic Inflammation ◽

Human Keratinocytes ◽

Epithelial Barrier ◽

Hacat Cells ◽

Initial Stage

Background/Aims: Calycosin is a bioactive component of Astragali Radix, a Chinese herb for treating allergy. We have previously demonstrated that calycosin effectively inhibited allergic inflammation efficiently. The aim of this study was to explore the mechanism of calycosin on epithelial cells in allergic inflammation. Methods: An initial stage of atopic dermatitis (AD) model in which mice were just sensitized with FITC, was established in vivo and immortalized human keratinocytes (HaCaT cells) were utilized in vitro. Initiative key cytokines, TSLP and IL-33, were measured by ELISA, qPCR, immunofluorescence and Western blot. The junctions in epithelial cells were observed by electron microscopy and tight junctions (TJs) (Occludin and ZO-1) were assessed by Western blot and immunofluorescence. TLR4, MyD88, TAK1, TIRAP and NF-κB were measured by qPCR or Western blot. Results: The results showed that TSLP and IL-33 were inhibited significantly by calycosin in the initial stage of AD model. Simultaneously, calycosin attenuated the separated gap among the epithelial cells and increased the expression of TJs. TSLP/IL-33 and TJs were similarly affected in LPS-stimulated HaCaT cells in vitro. Meanwhile, calycosin not only inhibited the expressions of TLR4, MyD88, TAK1 and TIRAP, but also reduced NF-κB activation in vitro and in vivo. An NF-κB inhibitor enhanced the expressions of TJs and reduced that of TSLP/IL-33 in LPS-stimulated HaCaT cells. Conclusion: These results indicated that calycosin reduced the secretion of TSLP/IL-33 and attenuated the disruption of epithelial TJs by inhibiting TLR4 mediated NF-κB signaling pathway. These findings help to understand the beneficial effects of calycosin on AD, and to develop effective preventive or therapeutic strategies to combat this disease and other epithelial barrier deletion-mediated allergic diseases.

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Surface Display of the Receptor-Binding Region of the Lactobacillusbrevis S-Layer Protein in Lactococcuslactis Provides Nonadhesive Lactococci with the Ability To Adhere to Intestinal Epithelial Cells

Applied and Environmental Microbiology ◽

10.1128/aem.69.4.2230-2236.2003 ◽

2003 ◽

Vol 69 (4) ◽

pp. 2230-2236 ◽

Cited By ~ 55

Author(s):

Silja Åvall-Jääskeläinen ◽

Agneta Lindholm ◽

Airi Palva

Keyword(s):

Cell Wall ◽

Lactic Acid ◽

Epithelial Cells ◽

Lactic Acid Bacterium ◽

Receptor Binding ◽

Surface Display ◽

Intestinal Epithelial ◽

Binding Region ◽

Receptor Binding Region

ABSTRACT Lactobacillus brevis is a promising lactic acid bacterium for use as a probiotic dietary adjunct and a vaccine vector. The N-terminal region of the S-layer protein (SlpA) of L. brevis ATCC 8287 was recently shown to mediate adhesion to various human cell lines in vitro. In this study, a surface display cassette was constructed on the basis of this SlpA receptor-binding domain, a proteinase spacer, and an autolysin anchor. The cassette was expressed under control of the nisA promoter in Lactococcus lactis NZ9000. Western blot assay of lactococcal cell wall extracts with anti-SlpA antibodies confirmed that the SlpA adhesion domain of the fusion protein was expressed and located within the cell wall layer. Whole-cell enzyme-linked immunosorbent assay and immunofluorescence microscopy verified that the SlpA adhesion-mediating region was accessible on the lactococcal cell surface. In vitro adhesion assays with the human intestinal epithelial cell line Intestine 407 indicated that the recombinant lactococcal cells had gained an ability to adhere to Intestine 407 cells significantly greater than that of wild-type L. lactis NZ9000. Serum inhibition assay further confirmed that adhesion of recombinant lactococci to Intestine 407 cells was indeed mediated by the N terminus-encoding part of the slpA gene. The ability of the receptor-binding region of SlpA to adhere to fibronectin was also confirmed with this lactococcal surface display system. These results show that, with the aid of the receptor-binding region of the L. brevis SlpA protein, the ability to adhere to gut epithelial cells can indeed be transferred to another, nonadhesive, lactic acid bacterium.

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Lactobacillus casei DN-114 001 Inhibits the Ability of Adherent-Invasive Escherichia coli Isolated from Crohn's Disease Patients To Adhere to and To Invade Intestinal Epithelial Cells

Applied and Environmental Microbiology ◽

10.1128/aem.71.6.2880-2887.2005 ◽

2005 ◽

Vol 71 (6) ◽

pp. 2880-2887 ◽

Cited By ~ 52

Author(s):

Isabelle Ingrassia ◽

Antony Leplingard ◽

Arlette Darfeuille-Michaud

Keyword(s):

Crohn’S Disease ◽

Crohn's Disease ◽

Epithelial Cells ◽

Lactobacillus Casei ◽

Intestinal Epithelial Cells ◽

Probiotic Strain ◽

Intestinal Cells ◽

Intestinal Epithelial ◽

Inhibitory Effects ◽

E Coli

ABSTRACT Ileal lesions in 36.4% of patients with Crohn's disease are colonized by pathogenic adherent-invasive Escherichia coli. The aim of this study was to determine the in vitro inhibitory effects of the probiotic strain, Lactobacillus casei DN-114 001, on adhesion to and invasion of human intestinal epithelial cells by adherent-invasive E. coli isolated from Crohn's disease patients. The experiments were performed with undifferentiated Intestine-407 cells and with undifferentiated or differentiated Caco-2 intestinal epithelial cells. Bacterial adhesion to and invasion of intestinal epithelial cells were assessed by counting CFU. The inhibitory effects of L. casei were determined after coincubation with adherent-invasive E. coli or after preincubation of intestinal cells with L. casei prior to infection with adherent-invasive E. coli. Inhibitory effects of L. casei on adherent-invasive E. coli adhesion to differentiated and undifferentiated intestinal epithelial cells reached 75% to 84% in coincubation and 43% to 62% in preincubation experiments, according to the cell lines used. Addition of L. casei culture supernatant to the incubation medium increased L. casei adhesion to intestinal epithelial cells and enhanced the inhibitory effects of L. casei. The inhibitory effects on E. coli invasion paralleled those on adhesion. This effect was not due to a bactericidal effect on adherent-invasive E. coli or to a cytotoxic effect on epithelial intestinal cells. As Lactobacillus casei DN-114 001 strongly inhibits interaction of adherent-invasive E. coli with intestinal epithelial cells, this finding suggests that the probiotic strain could be of therapeutic value in Crohn's disease.

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In vitro growth control of selected pathogens by Lactobacillus acidophilus- and Lactobacillus casei-fermented milk

Letters in Applied Microbiology ◽

10.1111/j.1472-765x.2006.02060.x ◽

2007 ◽

Vol 44 (3) ◽

pp. 314-319 ◽

Cited By ~ 50

Author(s):

M. Millette ◽

F.M. Luquet ◽

M. Lacroix

Keyword(s):

Lactobacillus Acidophilus ◽

Lactobacillus Casei ◽

Growth Control ◽

Fermented Milk ◽

In Vitro Growth

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Ultrastructure and cell division of an oral bacterium resembling Alysiella filiformis

Canadian Journal of Microbiology ◽

10.1139/m73-054 ◽

1973 ◽

Vol 19 (3) ◽

pp. 325-327 ◽

Cited By ~ 8

Author(s):

Gary E. Kaiser ◽

Marvin J. Starzyk

Keyword(s):

Cell Wall ◽

Cell Division ◽

Epithelial Cells ◽

Oral Cavity ◽

Structural Study ◽

Cell Separation ◽

Septum Formation ◽

Innermost Layer ◽

Oral Bacterium

Alysiella filiformis is commonly found on the epithelial cells of the oral cavity in rabbits. An ultra-structural study of these cells has shown A. filiformis attached by numerous slime appendages (setae) to the host epithelial cells. The organism possesses a multilayered cell wall 18–22 nm thick. Cell division occurs by constriction of the cytoplasm with concurrent septum formation initiating from the dense innermost layer of the cell wall. This is followed by thickening and delamination of the septum with subsequent invagination of the outer layers of the cell wall causing a partial cell separation. However, the cells of the typical trichomes are still held together by septal bridges. Mesosome-like structures were occasionally found and were often in the area of septum formation. All attempts to culture this organism in vitro were unsuccessful.

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