Effect of a Reactive Oxygen Species–Generating System for Control of Airborne Microorganisms in a Meat-Processing Environment†

2008 ◽  
Vol 71 (9) ◽  
pp. 1922-1925 ◽  
Author(s):  
J. R. PATEL ◽  
X. NOU
Keyword(s):  
Reactive Oxygen Species ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Reactive Oxygen ◽  
Control Treatment ◽  
Oxygen Species ◽  
Meat Processing ◽  
Log Reduction ◽  
Air Sampler ◽  
The Difference

The effectiveness of reactive oxygen species (ROS)–generating AirOcare equipment on the reduction of airborne bacteria in a meat-processing environment was determined. Serratia marcescens and lactic acid bacteria (Lactococcus lactis subsp. lactis and Lactobacillus plantarum) were used to artificially contaminate the air via a six-jet Collison nebulizer. Air in the meat-processing room was sampled immediately after aerosol generation and at various predetermined times at multiple locations by using a Staplex 6 stage air sampler. Approximately a 4-log reduction of the aerial S. marcescens population was observed within 2 h of treatment (P < 0.05) compared to a 1-log reduction in control samples. The S. marcescens populations reduced further by ~4.5 log after 24 h of exposure to ROS treatment. Approximately 3-log CFU/m3 reductions in lactic acid bacteria were observed following 2-h ROS exposure. Further ROS exposure reduced lactic acid bacteria in the air; however, the difference in their survival after 24 h of exposure was not significantly different from that observed with the control treatment. S. marcescens bacteria were more sensitive to ROS treatment than the lactic acid bacteria. These findings reveal that ROS treatment using the AirOcare unit significantly reduces airborne S. marcescens and lactic acid bacteria in meat-processing environments within 2 h.

scholarly journals Development and testing of an online method to measure ambient fine particulate reactive oxygen species (ROS) based on the 2',7'-dichlorofluorescin (DCFH) assay

2013 ◽  
Vol 6 (7) ◽  
pp. 1647-1658 ◽  
Author(s):  
L. E. King ◽  
R. J. Weber
Keyword(s):  
Reactive Oxygen Species ◽  
Limit Of Detection ◽  
Reactive Oxygen ◽  
Rural Site ◽  
Oxygen Species ◽  
Optimal Method ◽  
The Difference ◽  
Mist Chamber ◽  
Filter Particle ◽  
Urban Sites

Abstract. An online, semi-continuous instrument to measure fine particle (PM2.5) reactive oxygen species (ROS) was developed based on the fluorescent probe 2'7'-dichlorofluorescin (DCFH). Parameters that influence probe response were first characterized to develop an optimal method for use in a field instrument. The online method used a mist chamber scrubber to collect total (gas plus particle) ROS components (ROSt) alternating with gas phase ROS (ROSg) by means of an inline filter. Particle phase ROS (ROSp) was determined by the difference between ROSt and ROSg. The instrument was deployed in urban Atlanta, Georgia, USA, and at a rural site during various seasons. Concentrations from the online instrument generally agreed well with those from an intensive filter measurement of ROSp. Concentrations of the ROSp measurements made with this instrument were lower than reported in other studies, often below the instrument's average limit of detection (0.15 nmol H2O2 equivalents m−3). Mean ROSp concentrations were 0.26 nmol H2O2 equivalents m−3 at the Atlanta urban sites compared to 0.14 nmol H2O2 equivalents m−3 at the rural site.

scholarly journals Susceptibilities of lactoferrin and transferrin to myeloperoxidase-dependent loss of iron-binding capacity

1988 ◽  
Vol 250 (2) ◽  
pp. 613-616 ◽  
Author(s):  
C C Winterbourn ◽  
A L Molloy
Keyword(s):  
Reactive Oxygen Species ◽  
Binding Capacity ◽  
Reactive Oxygen ◽  
Oxidative Modification ◽  
Iron Binding ◽  
Oxygen Species ◽  
Protective Groups ◽  
The Difference ◽  
Iron Binding Capacity

Apolactoferrin and apotransferrin lost their ability to subsequently bind iron when exposed to an excess of either HOCl or myeloperoxidase plus H2O2 and Cl-. Apolactoferrin, however, was more resistant than apotransferrin. By oxidizing a mixture of the two proteins, then separating them by immunoprecipitation, the difference in susceptibility was shown to be due to the greater reactivity of transferrin iron-binding groups, rather than protective groups on the lactoferrin molecule. The iron-saturated proteins were much more resistant to oxidative modification than the apoproteins. The greater resistance of apolactoferrin should be advantageous for maintaining its iron binding capacity when co-released with myeloperoxidase and reactive oxygen species from stimulated neutrophils.

scholarly journals Reactive Oxygen Species Registration in Planarian Regeneration

2015 ◽  
Vol 7 (6) ◽  
pp. 13 ◽  
Author(s):  
Kh. P. Tiras ◽  
S. V. Gudkov ◽  
V. I. Emelyanenko ◽  
K. B. Aslanidi
Keyword(s):  
Reactive Oxygen Species ◽  
Physiological State ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Planarian Regeneration ◽  
The Difference ◽  
Dying Cells ◽  
First Time ◽  
Kinetics Of

<p class="1Body">Reactive oxygen species (ROS) are directly involved in cell proliferation, differentiation and apoptosis in a variety of organisms. We studied kinetics of own luminescence induced by changes of ROS in early stages of planarian regeneration. Kinetics of chemiluminescence were measured in intact planarians and the same individuals after decapitation within 15 hours. We analyzed the traumatic fluorescent signal obtained as the difference between kinetics of intact and decapitated planarians. It was found that regeneration is accompanied by changes in the content of ROS correlated with the energy-intensive process in regenerating planarians. Oxidative stress was caused by damage to cell membranes in the dissection of the planarian and it was accompanied by a drop in the intensity of luminescence with a time constant of about 3.6 hours. Phagocytosis of dying cells by neoblasts was accompanied by an increase of the luminescence intensity after 2 - 3 hours after decapitation. Neoblast mitosis was described by two maximums of luminescence over 5.1 hours and 8.3 hours after decapitation. For the first time we demonstrated the opportunity of registering the physiological state of pluripotent stem cells at the level of the organism <em>in vivo</em>.</p>

317 EFFECT OF REACTIVE OXYGEN SPECIES (ROS) ON ACTIVATION OF BOVINE OOCYTES MATURED IN VITRO

2006 ◽  
Vol 18 (2) ◽  
pp. 266 ◽  
Author(s):  
J. I. Park ◽  
Y. Jang ◽  
E. S. Lee
Keyword(s):  
Reactive Oxygen Species ◽  
Vitamin E ◽  
Chemical Activation ◽  
Calf Serum ◽  
Reactive Oxygen ◽  
Control Treatment ◽  
Oxygen Species ◽  
Cell Stage ◽  
Humidified Air

Vitamin E is an antioxidant that protects embryos from oxidative stress and supports embryonic development in vitro. This study was carried out to assess the effect of reactive oxygen species (ROS) by measuring the effects of hydrogen peroxide (H2O2) and vitamin E (VitE) on chemical activation of in vitro-matured oocytes. Bovine ovaries were collected from slaughtered cows at a local abattoir. Oocytes were aspirated from follicles 3-8 mm in diameter and transferred to maturation medium: tissue culture medium (TCM)-199 supplemented with 10% (v/v) fetal calf serum, 100 mg/L-cysteinr, 44 mg/L sodium pyruvate, gonadotropins (each 250 IU of eCG and hCG/mL), and 10 ng/mL epidermal growth factor. Oocytes were cultured at 38.9�C in 5% CO2 in humidified air. After 22 h of culture, oocytes with polar bodies were selected and submitted to activation treatments. Oocytes were exposed to calcium ionomycin (5 �M for 5 min) followed by incubation with 6-DMAP (2 mM) for 3.5 h in medium supplemented with or without VitE (100 �m). Routine in vitro fertilization (IVF) was also performed for 18 h as control treatment with or without VitE. After activation, oocytes were cultured in mSOF medium containing 0.8% BSA at 38.9�C in 5% CO2, 5% O2 in humidified air for 16-18 h. H2O2 content was measured and rate of development was monitored. For detection of H2O2, individual oocytes were stained with 22,72-dichlorofluorescein diacetate (DCFDA) and observed under fluorescence microscope (Hashimoto et al. 2000 Mol. Reprod. Dev. 56, 520-526). Levels of H2O2 were analyzed by counting the number of black-white pixels of TIFF images (ImageJ ver. 1.32; http://rsb.info.nih.gov/ij) after conversion of fluorescence image. Data were analyzed using Student's t-test and chi-square test. The H2O2 contents were significantly higher (P < 0.05) in the activation group without VitE (218 pixels, n = 49) than IVF without VitE (210 pixels, n = 50). Levels of H2O2 in oocytes activated with VitE were also higher (P < 0.05) than in the IVF group with VitE (216 pixels, n = 50 vs. 210 pixels, n = 51). Between groups with or without VitE, the H2O2 contents were not different in both activation and IVF. The cleavage rate to the 2-cell stage and development rate to blastocysts after activation with VitE (77.8% and 15.1%, n = 72) were significantly higher (P < 0.05) than in those without VitE (63.3% and 10.2%, n = 60). The results of the present study demonstrate that the chemical activation increased accumulation of H2O2 in oocytes compared to the IVF condition. Although ROS level did not affect chemical activation, vitamin E added in the activation medium promoted further development of activated embryos. This work was supported by a grant from the BioGreen 21 Program (20050301-034-443-026-03-00), Rural Development Administration, Korea.

scholarly journals The Promotion of Erythropoiesis via the Regulation of Reactive Oxygen Species by Lactic Acid

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Shun-Tao Luo ◽  
Dong-Mei Zhang ◽  
Qing Qin ◽  
Lian Lu ◽  
Min Luo ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Lactic Acid ◽  
Reactive Oxygen ◽  
Oxygen Species

Mitochondria as a source of reactive oxygen species

2009 ◽  
pp. c3 ◽  
Author(s):  
Helena M. Cochemé ◽  
Michael P. Murphy
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species
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