Prevalence of Arcobacter Species in Drinking Water, Spring Water, and Raw Milk as Determined by Multiplex PCR

2010 ◽  
Vol 73 (11) ◽  
pp. 2099-2102 ◽  
Author(s):  
NURHAN ERTAS ◽  
YUSUF DOGRUER ◽  
ZAFER GONULALAN ◽  
AHMET GUNER ◽  
ISMAIL ULGER
Keyword(s):  
Drinking Water ◽  
Membrane Filtration ◽  
Raw Milk ◽  
Spring Water ◽  
Potential Hazard ◽  
Environmental Waters ◽  
Milk Samples ◽  
Arcobacter Butzleri ◽  
Drinking Water Samples ◽  
Filtration Technique

The aim of this study was to investigate the incidence of Arcobacter species in water sources and raw milk from healthy animals in Kayseri, Turkey. A total of 175 samples of drinking water (n = 100), spring water (n = 25), and raw milk (n = 50) were examined. Arcobacter species were isolated using the membrane filtration technique. Overall, 7 (4%) of the 175 samples yielded Arcobacter spp.: 3 (3%) drinking water samples, 1 (4%) spring water sample, and 3 (6%) raw milk samples. Two species of Arcobacter were recovered from the seven positive samples: Arcobacter butzleri, Arcobacter skirrowii, and A. butzleri plus A. skirrowii found in 3 (1.7%), 2 (1.1%), and 2 (1.1%) samples, respectively. Our study is the first to report the isolation of both A. butzleri and A. skirrowii together from drinking water and is the first report of Arcobacter in milk from healthy cows in Turkey. Based on these findings, the presence of Arcobacter species in environmental waters and raw milk may pose a potential hazard for human health.

Arcobacter Species and Their Pulsed-Field Gel Electrophoresis Genotypes in Finnish Raw Milk during Summer 2011

2013 ◽  
Vol 76 (9) ◽  
pp. 1630-1632 ◽  
Author(s):  
JOANA REVEZ ◽  
MARIANNE HUUSKONEN ◽  
MARJO RUUSUNEN ◽  
MIIA LINDSTRÖM ◽  
MARJA-LIISA HÄNNINEN
Keyword(s):  
Gel Electrophoresis ◽  
Raw Milk ◽  
Positive Sample ◽  
Pulsed Field Gel Electrophoresis ◽  
Selective Detection ◽  
Potential Hazard ◽  
Pulsed Field ◽  
Milk Samples ◽  
Unpasteurized Milk ◽  
Arcobacter Butzleri

The aim of this study was to investigate the occurrence of Arcobacter species in raw milk in Finland. A total of 177 raw milk samples, each from a separate farm, were examined from June to August 2011. Arcobacter species were isolated using an enrichment and selective detection procedure. Overall, 26 (15%) of the 177 samples yielded Arcobacter spp. Samples from 25 farms were positive for Arcobacter butzleri and from 1 farm for Arcobacter cryaerophilus. Moreover, both Arcobacter butzleri and A. cryaerophilus were recovered from 1 positive sample. To evaluate a possible genetic variability, one strain of A. butzleri from each farm and the A. cryaerophilus sample were analyzed by pulsed-field gel electrophoresis. Genotyping revealed that Arcobacter spp. populations are heterogeneous, and no dominant clone has spread in the investigated samples. Our study is the first report on the isolation of both A. butzleri and A. cryaerophilus in raw milk in Finland. Based on our findings, the presence of Arcobacter species in raw milk may pose a potential hazard for human health, in particular for consumers who prefer drinking unpasteurized milk.

scholarly journals Aeromonas detection and their toxins from drinking water from reservoirs and drinking fountains

2007 ◽  
Vol 6 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Maria Tereza Pepe Razzolini ◽  
Marisa Di Bari ◽  
Petra Sanchez Sanchez ◽  
Maria Inês Zanoli Sato
Keyword(s):  
Drinking Water ◽  
Aquatic Ecosystems ◽  
Membrane Filtration ◽  
Biochemical Characterization ◽  
Toxin Production ◽  
Health Concern ◽  
Public Health Concern ◽  
Emerging Pathogen ◽  
Pathogenic Potential ◽  
Drinking Water Samples

Aeromonads are inhabitants of aquatic ecosystems and are described as being involved in intestinal disturbances and other infections. A total of 200 drinking water samples from domestic and public reservoirs and drinking fountains located in São Paulo (Brazil), were analyzed for the presence of Aeromonas. Samples were concentrated by membrane filtration and enriched in APW. ADA medium was used for Aeromonas isolation and colonies were confirmed by biochemical characterization. Strains isolated were tested for hemolysin and toxin production. Aeromonas was detected in 12 samples (6.0%). Aeromonas strains (96) were isolated and identified as: A. caviae (41.7%), A.hydrophila (15.7%), A.allosacharophila (10.4%), A. schubertii (1.0%) and Aeromonas spp. (31.2%).The results revealed that 70% of A. caviae, 66.7% of A. hydrophila, 80% of A. allosacharophila and 46.6% of Aeromonas spp. were hemolytic. The assay for checking production of toxins showed that 17.5% of A. caviae, 73.3% of A. hydrophila, 60% of A. allosacharophila, 100% of A. schubertii, and 33.3% of Aeromonas spp. were able to produce toxins. The results demonstrated the pathogenic potential of Aeromonas, indicating that the presence of this emerging pathogen in water systems is a public health concern.

Comparison of three different media for the detection of E. coli and coliforms in water

2006 ◽  
Vol 54 (3) ◽  
pp. 141-145 ◽  
Author(s):  
C. Bernasconi ◽  
G. Volponi ◽  
L. Bonadonna
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Membrane Filtration ◽  
Simultaneous Detection ◽  
Alternative Methods ◽  
Phenotypic Characteristic ◽  
Iso Standard ◽  
E Coli ◽  
Microbiological Parameters ◽  
Filtration Technique

The European Drinking Water Directive defines reference methods for the enumeration of microbiological parameters in drinking water. The method to be used for Escherichia coli and coliforms is the membrane filtration technique on Lactose TTC agar with Tergitol 7. Many technical drawbacks of the procedure, as well as its limitations regarding the recent taxonomy of coliforms, make it necessary to evaluate alternative methods. Two alternative assays, a chromogenic media (m-ColiBlu24®) and a defined substrate technology-DST test (Colilert 18/Quanty Tray™) were compared with the ISO standard with attention to the phenotypic characteristic of the isolates. Results showed that the ISO method failed to detect an important percentage of coliforms and E. coli while m-ColiBlu24® and Colilert 18 provided results in a shorter time allowing the simultaneous detection of E. coli and coliforms with no further confirmation steps.

scholarly journals Presence of Helicobacter pylori in drinking water of Karachi, Pakistan

2011 ◽  
Vol 6 (03) ◽  
pp. 251-255 ◽  
Author(s):  
Adnan Khan ◽  
Amber Farooqui ◽  
Shahana Urooj Kazmi
Keyword(s):  
Developing Countries ◽  
Helicobacter Pylori ◽  
Drinking Water ◽  
Environmental Factors ◽  
Metropolitan Area ◽  
Membrane Filtration ◽  
Filtration Method ◽  
Contaminated Drinking Water ◽  
H Pylori ◽  
Drinking Water Samples

Background: Prevalence of Helicobacter pylori (H. pylori) infection is an increasing problem in developing countries. Several environmental factors such as overcrowding, poverty, contaminated drinking water and food are considered to contribute toward transmission of infection; however, little is known about their definitive roles. The aim of this study is to investigate the presence of H. pylori in drinking water samples of Karachi, Pakistan. Methodology: Samples of drinking water were collected from 18 different towns located in the metropolitan area of Karachi. Samples were concentrated by membrane filtration method and subjected to PCR for the detection of H. pylori. Results: Two out of 50 (4%) samples collected from two different densely populated town areas were found to be positive for H. pylori. Conclusion: The study provides evidence for the presence of H. pylori in municipal drinking water of Karachi.

Prevalence of Arcobacter spp. in Raw Milk and Retail Raw Meats in Northern Ireland

2006 ◽  
Vol 69 (8) ◽  
pp. 1986-1990 ◽  
Author(s):  
ROISIN SCULLION ◽  
CLARE S. HARRINGTON ◽  
ROBERT H. MADDEN
Keyword(s):  
Northern Ireland ◽  
Raw Milk ◽  
Poultry Meat ◽  
Animal Origin ◽  
Diverse Range ◽  
Milk Samples ◽  
Specific Pcr ◽  
Arcobacter Butzleri ◽  
Species Specific ◽  
Raw Meats

A 1-year study was undertaken to determine the prevalence of Arcobacter spp. in raw milk and retail raw meats on sale in Northern Ireland. Retail raw poultry samples (n = 94), pork samples (n = 101), and beef samples (n = 108) were obtained from supermarkets in Northern Ireland, and raw milk samples (n = 101) were kindly provided by the Milk Research Laboratory, Department of Agriculture and Rural Development, Belfast, Northern Ireland. Presumptive arcobacters were identified by previously described genus-specific and species-specific PCR assays. Arcobacter spp. were found to be common contaminants of retail raw meats and raw milk in Northern Ireland. Poultry meat (62%) had the highest prevalence, but frequent isolations were made from pork (35%), beef (34%), and raw milk (46%). Arcobacter butzleri was the predominant species isolated from retail raw meats and was the only species isolated from raw milk samples. Arcobacter cryaerophilus was detected less frequently, and Arcobacter skirrowii was detected only as a cocontaminant. To our knowledge, this is the first report of Arcobacter spp. prevalence in a diverse range of products of animal origin in Northern Ireland.

scholarly journals Comparison of Membrane Filtration and Multiple-Tube Fermentation by the Colilert and Enterolert Methods for Detection of Waterborne Coliform Bacteria, Escherichia coli, and Enterococci Used in Drinking and Bathing Water Quality Monitoring in Southern Sweden

1998 ◽  
Vol 64 (8) ◽  
pp. 3079-3083 ◽  
Author(s):  
Karl F. Eckner
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Membrane Filtration ◽  
Water Quality Monitoring ◽  
Coliform Bacteria ◽  
Southern Sweden ◽  
Standard Methods ◽  
Bathing Water ◽  
Drinking Water Samples

ABSTRACT A total of 338 water samples, 261 drinking water samples and 77 bathing water samples, obtained for routine testing were analyzed in duplicate by Swedish standard methods using multiple-tube fermentation or membrane filtration and by the Colilert and/or Enterolert methods. Water samples came from a wide variety of sources in southern Sweden (Skåne). The Colilert method was found to be more sensitive than Swedish standard methods for detecting coliform bacteria and of equal sensitivity for detecting Escherichia coli when all drinking water samples were grouped together. Based on these results, Swedac, the Swedish laboratory accreditation body, approved for the first time in Sweden use of the Colilert method at this laboratory for the analysis of all water sources not falling under public water regulations (A-krav). The coliform detection study of bathing water yielded anomalous results due to confirmation difficulties. E. coli detection in bathing water was similar by both the Colilert and Swedish standard methods as was fecal streptococcus and enterococcus detection by both the Enterolert and Swedish standard methods.

A sensitive, semi-quantitative direct PCR-RFLP assay for simultaneous detection of five Cryptosporidium species in treated drinking waters and mineral waters

2002 ◽  
Vol 2 (3) ◽  
pp. 9-15 ◽  
Author(s):  
R.A.B. Nichols ◽  
C.A. Paton ◽  
B.M. Campbell ◽  
J. Wastling ◽  
H.V. Smith
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Membrane Filtration ◽  
Simultaneous Detection ◽  
Mineral Waters ◽  
Epifluorescence Microscopy ◽  
Direct Pcr ◽  
Species Identity ◽  
Pcr Rflp ◽  
Drinking Water Samples

We describe a semi-quantitative PCR-RFLP method for detecting low densities of Cryptosporidium spp. oocysts present in final drinking water samples and natural mineral waters. UK Standard Operating Protocols were used to concentrate oocysts from drinking water samples. Oocysts were concentrated from mineral waters by membrane filtration. Cryptosporidium oocysts identified by epifluorescence microscopy on slides or filters were subjected to DNA extraction and PCR-RFLP analysis. Oocysts were disrupted by freeze-thawing in lysis buffer. Amplicons were readily detected from 2 to 5 intact oocysts on ethidium bromide stained gels following 1 round of PCR. DNA extracted from C. parvum, C. muris, C. baileyi, human-derived C. meleagridis, and C. felis were used to confirm species identity by PCR-RFLP following simultaneous digestion with DraI and VspI.

Prevalence and Distribution of Arcobacter spp. in Raw Milk and Retail Raw Beef

2012 ◽  
Vol 75 (8) ◽  
pp. 1474-1478 ◽  
Author(s):  
A. H. SHAH ◽  
A. A. SALEHA ◽  
M. MURUGAIYAH ◽  
Z. ZUNITA ◽  
A. A. MEMON
Keyword(s):  
Dominant Species ◽  
Raw Milk ◽  
Cow’S Milk ◽  
First Report ◽  
Cow's Milk ◽  
Milk Samples ◽  
Goat’S Milk ◽  
Arcobacter Butzleri ◽  
Goat's Milk

A total of 106 beef samples which consisted of local (n = 59) and imported (n = 47) beef and 180 milk samples from cows (n = 86) and goats (n = 94) were collected from Selangor, Malaysia. Overall, 30.2% (32 of 106) of beef samples were found positive for Arcobacter species. Imported beef was significantly more contaminated (46.80%) than local beef (16.9%). Arcobacter butzleri was the species isolated most frequently from imported (81.8%) and local (60%) beef, followed by Arcobacter cryaerophilus in local (33.3%) and imported (18.2%) beef samples. Only one local beef sample (10%) yielded Arcobacter skirrowii. Arcobacter species were detected from cow's milk (5.8%), with A. butzleri as the dominant species (60%), followed by A. cryaerophilus (40%), whereas none of the goat's milk samples were found positive for Arcobacter. This is the first report of the detection of Arcobacter in milk and beef in Malaysia.

Sign in / Sign up

Export Citation Format

Share Document