scholarly journals Comparison of Membrane Filtration and Multiple-Tube Fermentation by the Colilert and Enterolert Methods for Detection of Waterborne Coliform Bacteria, Escherichia coli, and Enterococci Used in Drinking and Bathing Water Quality Monitoring in Southern Sweden

1998 ◽  
Vol 64 (8) ◽  
pp. 3079-3083 ◽  
Author(s):  
Karl F. Eckner
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Membrane Filtration ◽  
Water Quality Monitoring ◽  
Coliform Bacteria ◽  
Southern Sweden ◽  
Standard Methods ◽  
Bathing Water ◽  
Drinking Water Samples

ABSTRACT A total of 338 water samples, 261 drinking water samples and 77 bathing water samples, obtained for routine testing were analyzed in duplicate by Swedish standard methods using multiple-tube fermentation or membrane filtration and by the Colilert and/or Enterolert methods. Water samples came from a wide variety of sources in southern Sweden (Skåne). The Colilert method was found to be more sensitive than Swedish standard methods for detecting coliform bacteria and of equal sensitivity for detecting Escherichia coli when all drinking water samples were grouped together. Based on these results, Swedac, the Swedish laboratory accreditation body, approved for the first time in Sweden use of the Colilert method at this laboratory for the analysis of all water sources not falling under public water regulations (A-krav). The coliform detection study of bathing water yielded anomalous results due to confirmation difficulties. E. coli detection in bathing water was similar by both the Colilert and Swedish standard methods as was fecal streptococcus and enterococcus detection by both the Enterolert and Swedish standard methods.

scholarly journals DETEKSI BAKTERI Escherichia coli DALAM AIR MINUM ISI ULANG YANG DISTERILISASI ULTRAVIOLET DI WILAYAH KECAMATAN JAGAKARSA

Jurnal BIOMA ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 73
Author(s):  
Rezki Rachmawati ◽  
Muzajjanah Muzajjanah ◽  
Yoswita Rustam
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Coliform Bacterium ◽  
Probable Number ◽  
E Coli ◽  
Biological Contamination ◽  
Drinking Water Samples

Refill Water Depot is currently more widely circulated and used as an alternative drinking water supply by the public. However the still unclear about the quality of the drinking water refill generated primarily of biological content. Parameters of biological contamination in drinking water caused by the Escherichia coli and coliform bacterium. This study aims to identify E. coli and coliforms in drinking water refill. Refill drinking water samples obtained from 16 drinking water refill from Jagakarsa subdsitrict. The method used is descriptive. Refill drinking water samples was taken and tested in the MPN (Most Probable Number) method and then to be tested in identification of E. coli. The results of testing the drinking water refill obtained 15 samples positive for coliform bacteria. Samples were positive for E. coli bacteria that sample B.1 and F.2.

scholarly journals Identifikasi Bakteri Escherichia coli dalam Air Minum Galon pada Kantin yang ada di Universitas Andalas Padang

2021 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Muhammad Rayhan Braja Gitawama ◽  
Netti Suharti ◽  
Nora Harminarti
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Data Analysis ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Drug Agency ◽  
Drinking Water Samples

The National Food and Drug Agency has controlled the refill drinking water in drinking water depots and also controlled food at school canteens ranging from elementary, junior high, to high school, but the National Food and Drug Agency never do food quality controlling in canteens at universities. Objectives: To identified the contamination by coliform and E. coli bacteria in drinking water at Andalas University canteen. Methods: This research was descriptive to identify coliform bacteria on  15 drinking water at the Andalas University faculty canteens.  Samples were taken directly using a sterile bottle, while data analysis using Most Probable Number  (MPN)  tables 5-1-1 and the presence of E. coli bacteria colonies from drinking water samples. Results: 9 of 15 water samples were contaminated by coliform bacteria with the highest MPN index of 240/100 ml that was found in 2 samples. From 9 samples containing coliform bacteria, all of them were found to contain E. coli bacteria. Conclusion: Most of the samples were contaminated by coliform and E. coli bacteria. Drinking water served using a kettle was more contaminated than drinking water served using gallons.Keywords: Coliform, Escherichia coli, MPN

scholarly journals DETEKSI BAKTERI Escherichia coli DALAM AIR MINUM ISI ULANG YANG DISTERILISASI ULTRAVIOLET DI WILAYAH KECAMATAN JAGAKARSA

Jurnal BIOMA ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 73
Author(s):  
Rezki Rachmawati ◽  
Muzajjanah Muzajjanah ◽  
Yoswita Rustam
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Coliform Bacterium ◽  
Probable Number ◽  
E Coli ◽  
Biological Contamination ◽  
Drinking Water Samples

Refill Water Depot is currently more widely circulated and used as an alternative drinking water supply by the public. However the still unclear about the quality of the drinking water refill generated primarily of biological content. Parameters of biological contamination in drinking water caused by the Escherichia coli and coliform bacterium. This study aims to identify E. coli and coliforms in drinking water refill. Refill drinking water samples obtained from 16 drinking water refill from Jagakarsa subdsitrict. The method used is descriptive. Refill drinking water samples was taken and tested in the MPN (Most Probable Number) method and then to be tested in identification of E. coli. The results of testing the drinking water refill obtained 15 samples positive for coliform bacteria. Samples were positive for E. coli bacteria that sample B.1 and F.2.

scholarly journals Microbiological Analysis of Drinking Water of Kathmandu Valley

1970 ◽  
Vol 5 (5) ◽  
pp. 112-114 ◽  
Author(s):  
Tista Prasai ◽  
Binod Lekhak ◽  
Dev Raj Joshi ◽  
Madhav Prasad Baral
Keyword(s):  
Public Health ◽  
Drinking Water ◽  
Water Samples ◽  
Enteric Bacteria ◽  
Coliform Bacteria ◽  
Kathmandu Valley ◽  
Microbiological Analysis ◽  
Public Health Importance ◽  
Guideline Value ◽  
Drinking Water Samples

Drinking water quality assessment in Kathmandu valley has always been crucial with reference to public health importance. A study was conducted to evaluate the quality of drinking water of the valley. A total of 132 drinking water samples were randomly collected from 49 tube wells, 57 wells, 17 taps and 9 stone spouts in different places of Kathmandu valley. The samples were analyzed for microbiological parameters. Total plate and coliform count revealed that 82.6% and 92.4% of drinking water samples found to cross the WHO guideline value for drinking water. During the study, 238 isolates of enteric bacteria were identified, of which 26.4% were Escherichia coli, 25.6% were Enterobacter spp, 23% were Citrobacter spp, 6.3% were Pseudomonas aeruginosa, 5.4% were Klebsiella spp, 4.0% were Shigella spp, 3.0% were Salmonella typhi, 3.0% were Proteus vulgaris, 3.0% were Serratia spp and 1.0% were Vibrio cholerae . Key words: Drinking water; Public health; Coliform; Bacteria. DOI: 10.3126/sw.v5i5.2667 Scientific World, Vol. 5, No. 5, July 2007 112-114

scholarly journals Comparison of Colilert-18 with miniaturised most probable number method for monitoring of Escherichia coli in bathing water

2015 ◽  
Vol 14 (1) ◽  
pp. 121-131 ◽  
Author(s):  
Ananda Tiwari ◽  
Seppo I. Niemelä ◽  
Asko Vepsäläinen ◽  
Jarkko Rapala ◽  
Seija Kalso ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
False Negative ◽  
Reference Method ◽  
Water Quality Monitoring ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Negative Results ◽  
Bathing Water

The purpose of this equivalence study was to compare an alternative method, Colilert-18 Quanti-Tray (ISO 9308-2) with the European bathing water directive (2006/7/EC) reference method, the miniaturised most probable number (MMPN) method (ISO 9308-3), for the analysis of Escherichia coli. Six laboratories analysed a total of 263 bathing water samples in Finland. The comparison was carried out according to ISO 17994:2004. The recovery of E. coli using the Colilert-18 method was 7.0% and 8.6% lower than that of the MMPN method after 48 hours and 72 hours of incubation, respectively. The confirmation rate of presumptive E. coli-positive wells in the Colilert-18 and MMPN methods was high (97.8% and 98.0%, respectively). However, the testing of presumptive E. coli-negative but coliform bacteria-positive (yellow but not fluorescent) Colilert-18 wells revealed 7.3% false negative results. There were more false negatives in the naturally contaminated waters than in the samples spiked with waste water. The difference between the recovery of Colilert-18 and the MMPN method was considered not significant, and subsequently the methods are considered as equivalent for bathing water quality monitoring in Finland. Future bathing water method equivalence verification studies may use the data reported herein. The laboratories should make sure that any wells showing even minor fluorescence will be determined as positive for E. coli.

scholarly journals Identifikasi Bakteri Escherichia coli pada Air Minum Isi Ulang yang Diproduksi DAMIU di Kelurahan Lubuk Buaya Kota Padang

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Rani Afrisetiawati ◽  
Erly Erly ◽  
Endrinaldi Endrinaldi
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Drinking Water ◽  
Laboratory Test ◽  
Human Body ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Bacteriological Test ◽  
Identification Test

AbstrakAir merupakan  komponen penting bagi tubuh.  Menurut Permenkes No.492 Tahun 2010 tentang syarat mikrobiologi air minum adalah tidak ditemukannya bakteri Coliform dan Escherichia coli dalam 100 ml air. Tujuan penelitian ini adalah mengetahui kualitas air minum yang diproduksi depot air minum isi ulang di Kelurahan Lubuk Buaya. Penelitian bersifat deskriptif dengan melakukan pemeriksaan bakteriologi sampel air di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas dari Februari  sampai Oktober 2014. Sampel adalah air dari masing-masing depot air minum isi ulang di Kelurahan Lubuk Buaya yang sudah dimasukkan ke dalam galon. Penelitian ini terdiri dari empat tes, yaitu tes presumtif, konfirmatif, lengkap dan identifikasi. Hasil pemeriksaan laboratorium menunjukkan empat dari dua belas sampel (33,3%) mengandung bakteri Coliform dan satu dari empat sampel tersebut mengandung Escherichia coli. Pengawasan terhadap depot air minum isi ulang di Kelurahan Lubuk Buaya harus lebih ditingkatkan. Kader Kesehatan setempat diharapkan dapat memberikan penyuluhan kepada pemilik atau operator depot agar memperhatikan kebersihan depot serta peralatan yang digunakan.Kata kunci: air minum isi ulang, Coliform, Escherichia  coli AbstractWater is an essential component for the human body. According to Permenkes No. 492 Tahun 2010 about microbiological standards for drinking water is the absence of coliform and Escherichia coli in 100 ml of water. The objective of this study was to find the quality of drinking water produced by drinking water refill depots at Lubuk Buaya Padang based on microbiological standards of drinking water.The research was conducted using by bacteriological test on 12 water samples at the Laboratory of Microbiology, Facultyof Medicine, University of Andalas from February until Oktober 2014. This research consisted  four tests: the presumptive test, confirmative test, completetest and identification test. Based on the result of laboratory test, there were four samples (33,3%) from twelve samples contain Coliform bacteria and one of them contain Escherichia coli. Controlling of drinking water refill depots at Lubuk Buaya should have been improved. The officers of Public Health at Lubuk Buaya must give an advice to the owners or depot operators about hygiene of depot and tools that they used.Keywords: drinking water refill, Coliform, Escherichia coli

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