scholarly journals Histological evaluation of bone repair using beta-Tricalcium Phosphate

2011 ◽  
pp. e190-e194 ◽  
Author(s):  
R. Frota ◽  
VA. Silva-Junior ◽  
M. Teixeira ◽  
AP. Veras-Sobral ◽  
E. Silva ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Bone Repair ◽  
Histological Evaluation ◽  
Beta Tricalcium Phosphate

scholarly journals Histological Evaluation of a New Beta-Tricalcium Phosphate/Hydroxyapatite/Poly (1-Lactide-Co-Caprolactone) Composite Biomaterial in the Inflammatory Process and Repair of Critical Bone Defects

Symmetry ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1356
Author(s):  
Elizabeth Ferreira Martinez ◽  
Ana Elisa Amaro Rodrigues ◽  
Lucas Novaes Teixeira ◽  
Andrea Rodrigues Esposito ◽  
Walter Israel Rojas Cabrera ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Tricalcium Phosphate ◽  
Bone Repair ◽  
Inflammatory Process ◽  
Histological Evaluation ◽  
Alloplastic Material ◽  
Beta Tricalcium Phosphate ◽  
Evaluation Time ◽  
Synthetic Biomaterial ◽  
Critical Bone Defects

Background: The use of biomaterials is commonplace in dentistry for bone regeneration. The aim of this study was to evaluate the performance of a new alloplastic material for bone repair in critical defects and to evaluate the extent of the inflammatory process. Methods: Forty-five New Zealand rabbits were divided into five groups according to evaluation time (7, 14, 30, 60, 120 days), totaling 180 sites with six-millimeter diameter defects in their tibiae. The defects were filled with alloplastic material consisting of poly (lactide-co-caprolactone), beta-tricalcium phosphate, hydroxyapatite and nano-hydroxyapatite (BTPHP) in three different presentations: paste, block, and membrane. Comparisons were established with reference materials, such as Bio-ossTM, Bio-oss CollagenTM, and Bio-gideTM, respectively. The samples were HE-stained and evaluated for inflammatory infiltrate (scored for intensity from 0 to 3) and the presence of newly formed bone at the periphery of the defects. Results: Greater bone formation was observed for the alloplastic material and equivalent inflammatory intensity for both materials, regardless of evaluation time. At 30 days, part of the synthetic biomaterial, regardless of the presentation, was resorbed. Conclusions: We concluded that this novel alloplastic material showed osteoconductive potential, biocompatibility, low inflammatory response, and gradual resorption, thus an alternative strategy for guided bone regeneration.

Fabrication and biological evaluation of porous β-TCP bioceramics produced using digital light processing

2021 ◽  
pp. 095441192110411
Author(s):  
SongFeng Xu ◽  
Hang Zhang ◽  
Xiang Li ◽  
XinXin Zhang ◽  
HuanMei Liu ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Bone Repair ◽  
Femoral Condyle ◽  
Biological Evaluation ◽  
Mechanical Tests ◽  
Digital Light Processing ◽  
Beta Tricalcium Phosphate ◽  
Ectopic Bone ◽  
Good Biocompatibility ◽  
Mouse Osteoblast

Beta-tricalcium phosphate ( β-TCP) refers to one ideal bone repair substance with good biocompatibility and osteogenicity. A digital light processing (DLP)-system used in this study creates bioceramic green part by stacking up layers of photocurable tricalcium phosphate-filled slurry with various β-TCP weight fractions. Results show that the sintering shrinkage is anisotropic and the shrinkage vertically reaches over that horizontally. The obtained porous β-TCP parts have both macroporous outer structure and microporous inner structure, the macropore size is 400–600 μm and the micropore size is 500–1500 nm. The mechanical tests show that the porous β-TCP bioceramic’s compressive strength reaches 16.53 MPa. The cell culture confirmed that the porous β-TCP bioceramic is capable of achieving the effective attaching, growing, and proliferating pertained to mouse osteoblast cells. This study identified considerable blood vessels and significant ectopic bone forming obviously based on the histologically-related assessment when implanting to rabbit femoral condyle deficiency for 3 months. Thus, under high bioactive property and osteoinductivity, and large precision and mechanical strength that can be adjusted, the DLP printed porous β-TCP ceramics is capable of being promising for special uses of bones repairing.

Repairing of Osteochondral Defects in Joint Using Beta-TCP/ Carboxymethyl Chitin Composite

2005 ◽  
Vol 284-286 ◽  
pp. 791-794 ◽  
Author(s):  
Shingo Masuda ◽  
Yusuke Yoshihara ◽  
Kazuaki Muramatsu ◽  
Izumi Wakebe
Keyword(s):  
Mechanical Properties ◽  
Articular Cartilage ◽  
Tricalcium Phosphate ◽  
Bone Repair ◽  
Bone Defects ◽  
Promising Material ◽  
Osteochondral Defects ◽  
Repair Rate ◽  
Beta Tricalcium Phosphate

Beta-tricalcium phosphate/carboxymethyl chitin composites [TCP/CMCh] of various ratios of TCP granules and CMCh were made and their mechanical properties, handling properties and repair performance for bone defects and for osteochondral defects were investigated. Water pooling ratio of CMCh was approximately 40 times the weight itself. TCP/CMCh of a higher TCP ratio had higher stress at 50%-strain. The stress at 50%-strain of TCP/CMCh with 0, 2.5, 5.0, 7.5, 10 TCP ratios was 0.12, 0.51, 1.08, 1.46, 1.67 (MPa, n=5), respectively. The TCP/CMCh with 5.0 TCP ratios had the best total scores in handling tests. The bone repair rate of TCP/CMCh was TCP ratio 2.5< Blank= TCP ratio 7.5< TCP ratio 5.0. In the implantation test for osteochondral defects, TCP/CMCh was completely absorbed at four weeks after surgery. Regeneration of the articular cartilage was seen with TCP/CMCh and HA/CMCh but not with TCP granules, which remained eight weeks after implantation. The regenerated articular cartilage had remained 32 weeks after implantation. In conclusion, it was demonstrated that this TCP/CMCh composite was a promising material for repairing osteochondral defects.

scholarly journals Evaluation of Bone Repair in the Mandible of Rabbits Using Biphasic Calcium Phosphate Micro-Macroporous Hydroxyapatite Bioceramics and Beta-Tricalcium Phosphate

2019 ◽  
Vol 19 (1) ◽  
pp. 1-12
Author(s):  
Francisco Franceschini Neto ◽  
Rudyard dos Santos Oliveira ◽  
Ana Paula Altheman Lopes ◽  
Carlos Eduardo Xavier dos Santos Ribeiro da Silva
Keyword(s):  
Calcium Phosphate ◽  
Tricalcium Phosphate ◽  
Biphasic Calcium Phosphate ◽  
Bone Repair ◽  
Beta Tricalcium Phosphate

Preparation and Properties of Porous β–Tricalcium Phosphate Bone Graft

2012 ◽  
Vol 624 ◽  
pp. 226-230 ◽  
Author(s):  
Li Sheng Zhao ◽  
Zheng Wang ◽  
Ke Ya Mao ◽  
Bin Deng ◽  
Yuan Fu Yi ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Porous Structure ◽  
Bone Graft ◽  
Cancellous Bone ◽  
Tricalcium Phosphate ◽  
Bone Repair ◽  
X Ray Diffraction ◽  
X Ray ◽  
Beta Tricalcium Phosphate ◽  
Similar Chemical

The need for bone repair has increased as the population ages. However, currently, the bone grafts still have some disadvantages, such as low compressive strength and porosity, which limit their use. In order to solve these disadvantages, in this study, the porous beta-tricalcium phosphate (β-TCP) anorganic bone graft were prepared from healthy bovine cancellous bone by cell-free, defat and twice calcinations. X-ray diffraction (XRD) was used to investigate the chemical composition of the bone graft. And the morphology, porosity and mechanical strength of the bone graft were also evaluated. The results showed that most constituent of the bone graft was β-TCP. In addition, the bone graft scaffold exhibited the macro and micro porous structure and the porosity was 57.63%, just as the nature cancellous bone. The compressive strength was 4.47±0.63MPa. Above all, the porous β-TCP anorganic bone graft not only has similar chemical composites as the nature cancellous bone, but also it can effectively retain the porous structure of natural cancellous bone and provides optimal channels for the ingrowth of new bone and blood vessels.Therefore, the porous β-TCP anorganic bone graft is a potential biomaterial in bone tissue engineering.

Study of Bone Repair in Rat Dental Socket after Implantation of Porous Granules of Beta-Tricalcium Phosphate (β-TCP) and Magnesium-Substituted Beta-Tricalcium Phosphate (β-TCMP)

2011 ◽  
Vol 493-494 ◽  
pp. 263-268 ◽  
Author(s):  
Debora Helena Yassuda-Mattos ◽  
Neusa Motta de Freitas Costa ◽  
Débora dos Santos Tavares ◽  
G.V.O. Fernandes ◽  
A. Alves ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Sodium Alginate ◽  
Tricalcium Phosphate ◽  
Bone Repair ◽  
Neutrophil Granulocytes ◽  
Group Iii ◽  
Multinucleated Cells ◽  
Fourier Transformed Infrared Spectroscopy ◽  
Beta Tricalcium Phosphate ◽  
Group I

Porous granules were obtained through a route using a mixture of calcium phosphate powder with sodium alginate solution. The sintered β-TCP and β-TCMP granules with diameters ranging from 250 µm to 500 µm were implanted into dental alveoli of 30 rats (Rattus norvegicus). The animals were divided in three groups: group I (control, no treatment), group II (β-TCP) and group III (β-TCMP); the sacrifices occurred at 7 and 21 days (n=5/group/period). Histological and histomorphometric analysis were performed to observe and measure connective tissue, bone neoformation and biomaterial areas. Most animals showed acute inflammatory response with many neutrophil granulocytes and foreign body giant multinucleated cells associated to both biomaterials, at 7 and 21 days. The utilization of sodium alginate as additive in the porous granules might explain these results. Fourier-transformed infrared spectroscopy (FTIR) pointed out residue in the granules surface that could exacerbate the inflammatory response. Additional studies are in course to confirm such hypothesis.

scholarly journals Current Application of Beta-Tricalcium Phosphate in Bone Repair and Its Mechanism to Regulate Osteogenesis

2021 ◽  
Vol 8 ◽  
Author(s):  
Haiping Lu ◽  
Yinghong Zhou ◽  
Yaping Ma ◽  
Lan Xiao ◽  
Wenjun Ji ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Loss ◽  
Tricalcium Phosphate ◽  
Bone Repair ◽  
Blood Clot ◽  
Current Evidence ◽  
Angiogenic Growth Factors ◽  
Current Application ◽  
Beta Tricalcium Phosphate

Large segmental bone loss and bone resection due to trauma and/or the presence of tumors and cysts often results in a delay in healing or non-union. Currently, the bone autograft is the most frequently used strategy to manage large bone loss. Nevertheless, autograft harvesting has limitations, namely sourcing of autograft material, the requirement of an invasive procedure, and susceptibility to infection. These disadvantages can result in complications and the development of a bone substitute materials offers a potential alternative to overcome these shortcomings. Among the biomaterials under consideration to date, beta-tricalcium phosphate (β-TCP) has emerged as a promising material for bone regeneration applications due to its osteoconductivity and osteoinductivity properties as well as its superior degradation in vivo. However, current evidence suggests the use β-TCP can in fact delay bone healing and mechanisms for this observation are yet to be comprehensively investigated. In this review, we introduce the broad application of β-TCP in tissue engineering and discuss the different approaches that β-TCP scaffolds are customized, including physical modification (e.g., pore size, porosity and roughness) and the incorporation of metal ions, other materials (e.g., bioactive glass) and stem cells (e.g., mesenchymal stem cells). 3D and 4D printed β-TCP-based scaffolds have also been reviewed. We subsequently discuss how β-TCP can regulate osteogenic processes to aid bone repair/healing, namely osteogenic differentiation of mesenchymal stem cells, formation of blood vessels, release of angiogenic growth factors, and blood clot formation. By way of this review, a deeper understanding of the basic mechanisms of β-TCP for bone repair will be achieved which will aid in the optimization of strategies to promote bone repair and regeneration.

scholarly journals Liposomal clodronate inhibition of osteoclastogenesis and osteoinduction by submicrostructured beta-tricalcium phosphate

Biomaterials ◽  
2014 ◽  
Vol 35 (19) ◽  
pp. 5088-5097 ◽  
Author(s):  
Noel L. Davison ◽  
Anne-Laure Gamblin ◽  
Pierre Layrolle ◽  
Huipin Yuan ◽  
Joost D. de Bruijn ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Beta Tricalcium Phosphate ◽  
Liposomal Clodronate
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