scholarly journals Protective role of caffeine versus N-acetylcysteine in hyperoxic acute lung injury in neonatal rats

2017 ◽  
Vol 34 (02) ◽  
pp. 058-067
Author(s):  
A. Sadek ◽  
R. Khattab ◽  
A. Amer ◽  
A. Youssef
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Immune Reaction ◽  
Interleukin 1 ◽  
Protective Role ◽  
High Oxygen ◽  
Control Group ◽  
Albino Rats ◽  
Neonatal Rats

Abstract Introduction: Prolonged breathing of high oxygen concentration leads to hyperoxic acute lung injury. Neonatal Respiratory diseases usually require increased supplement of high oxygen concentrations, so neonates are more susceptible to hyperoxic acute lung injury. The aim of this work was to investigate the protective role of caffeine versus N-acetylcysteine against hyperoxic acute lung injury in neonatal rats. Materials and Methods: 32 albino rats aged seven days were used in this experiment. The pups were divided into four groups; 1) Control or normoxic group; rats placed in normoxic chamber where fraction of inspired oxygen (FiO2) was 0.21, 2) Hyperoxic group; rats were placed in hyperoxic chamber (FiO2>0.8) using an oxygen flow of 1.5 Litre/min, 3) Hyperoxia-CAF group; rats exposed to hyperoxia and received a single intra-peritoneal injection of 20 mg/kg caffeine just prior to exposure, and 4) Hyperoxia-NAC group; rats exposed to hyperoxia and received a single intra-peritoneal injection of 150 mg/kg N-acetylcysteine just prior to exposure. 48 hours after exposure, lung specimens were processed for histological and immunohistochemical study using caspase-3, cluster of differentiation-68-antibody (CD68) and interleukin-1-beta (IL-1β). Results: Neonatal hyperoxia led to severe impairment in lung architecture, with a highly significant increase in alveolar macrophages. Also, caspase and IL-1β immune-reaction were increased significantly as compared to control group. Caffeine could improve the histolopathological picture of hyperoxic acute lung injury, and also could decrease alveolar macrophage count and IL-1β immune-reaction better than N-acetylcysteine. Conclusion: Caffeine is more effective than N-acetylcysteine in prophylaxis against hyperoxic acute lung injury in neonates.

Effects of erdosteine on cyclosporin-A-induced nephrotoxicity

2011 ◽  
Vol 31 (6) ◽  
pp. 565-573 ◽  
Author(s):  
M Tutanc ◽  
V Arica ◽  
N Yılmaz ◽  
A Nacar ◽  
I Zararsiz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cyclosporin A ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Protective Mechanism ◽  
Antioxidant Parameters ◽  
Group 2 ◽  
Wistar Albino Rats

Aim: In cyclosporin-A (CsA)-induced toxicity, oxidative stress has been implicated as a potential responsible mechanism. Therefore, we aimed to investigate the protective role of erdosteine against CsA-induced nephrotoxicity in terms of tissue oxidant/antioxidant parameters and light microscopy in rats. Materials and methods: Wistar albino rats were randomly separated into four groups. Group 1 rats treated with sodium chloride served as the control, group 2 rats were treated with CsA, group 3 with CsA plus erdosteine, and group 4 with erdosteine alone. Animals were killed and blood samples were analyzed for blood urea nitrogen (BUN), serum creatinine (Cr), uric acid (UA), total protein (TP), and albumin (ALB) levels. Kidney sections were analyzed for malondialdehyde (MDA) and nitric oxide (NO) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities, as well as for histopathological changes. Results: In the CsA group, MDA, GSH-Px, BUN, and Cr levels were increased. The TP and ALB levels were decreased. These changes had been improved by erdosteine administration. Other biochemical parameters did not show any significant change. Conclusion: These results indicate that erdosteine produces a protective mechanism against CsA-induced nephrotoxicity and suggest a role of oxidative stress in pathogenesis.

scholarly journals Protective role of Nigella sativa in chemotherapy-induced alopecia

2017 ◽  
Vol 12 (4) ◽  
pp. 455
Author(s):  
Uzma Saleem ◽  
Shakila Sabir ◽  
Bashir Ahmad
Keyword(s):  
Nigella Sativa ◽  
Video Clip ◽  
Protective Role ◽  
Hair Follicles ◽  
Control Group ◽  
Albino Rats ◽  
Adult Rat ◽  
Anagen Phase ◽  
Full Screen

<p>Chemotherapy-induced alopecia affects the pathological as well as the psychological aspects of the cancer patient. In the present study, the protective role of Nigella sativa was evaluated in both adult and newborn albino rats. The anagen phase was first induced. N. sativa oil, N. sativa decoction (5%, 10% and 15%) and minoxidil lotion (standard) were applied daily to the rats two days after the depilation. During the anagen VI phase of the hair follicles, alopecia was induced by giving cyclophosphamide 125 mg/kg, ip to the adult rat and 50 mg/kg to the newborn rats. Cyclophosphamide-induced the alopecia to the whole depilated area of skin in adult rat while alopecia totalis was observed in the newborn rat disease control group. N. sativa oil, N. sativa decoction (5%) showed a significant protective effect against cyclophosphamide-induced alopecia. In conclusion, it is evident that N. sativa provides significant protection against chemotherapy-induced alopecia.</p><p><strong>Video Clip of Methodology</strong>:</p><p>1 min 43 sec:   <a href="https://www.youtube.com/v/AKhk27V3juE">Full Screen</a>   <a href="https://www.youtube.com/watch?v=AKhk27V3juE">Alternate</a></p>

Respiratory Defense Mechanisms. The Protective Role of Thioredoxin/ADF in Bleomycin-induced, Acute Lung Injury.

1998 ◽  
Vol 49 (2) ◽  
pp. 109-112
Author(s):  
Shu Hashimoto ◽  
Yasuhiro Gon ◽  
Jyunji Yodoi ◽  
Takashi Horie
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Defense Mechanisms ◽  
Protective Role

Legionella-induced acute lung injury in the setting of hyperoxia: protective role of tumour necrosis factor-α

2004 ◽  
Vol 53 (8) ◽  
pp. 727-733 ◽  
Author(s):  
Chiharu Nara ◽  
Kazuhiro Tateda ◽  
Tetsuya Matsumoto ◽  
Akira Ohara ◽  
Shuichi Miyazaki ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Tumour Necrosis Factor ◽  
Tumour Necrosis ◽  
Protective Role ◽  
Tumour Necrosis Factor Α ◽  
Factor Α ◽  
Necrosis Factor

scholarly journals Protective Role of Proton-Sensing TDAG8 in Lipopolysaccharide-Induced Acute Lung Injury

2015 ◽  
Vol 16 (12) ◽  
pp. 28931-28942 ◽  
Author(s):  
Hiroaki Tsurumaki ◽  
Chihiro Mogi ◽  
Haruka Aoki-Saito ◽  
Masayuki Tobo ◽  
Yosuke Kamide ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Protective Role

scholarly journals Oxidative stress-induced FABP5 S-glutathionylation protects against acute lung injury by suppressing inflammation in macrophages

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yuxian Guo ◽  
Yaru Liu ◽  
Shihao Zhao ◽  
Wangting Xu ◽  
Yiqing Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Target Genes ◽  
Nuclear Translocation ◽  
Protein S ◽  
Protective Role ◽  
Fatty Acid Binding ◽  
Proteomics Approach

AbstractOxidative stress contributes to the pathogenesis of acute lung injury. Protein S-glutathionylation plays an important role in cellular antioxidant defense. Here we report that the expression of deglutathionylation enzyme Grx1 is decreased in the lungs of acute lung injury mice. The acute lung injury induced by hyperoxia or LPS is significantly relieved in Grx1 KO and Grx1fl/flLysMcre mice, confirming the protective role of Grx1-regulated S-glutathionylation in macrophages. Using a quantitative redox proteomics approach, we show that FABP5 is susceptible to S-glutathionylation under oxidative conditions. S-glutathionylation of Cys127 in FABP5 promotes its fatty acid binding ability and nuclear translocation. Further results indicate S-glutathionylation promotes the interaction of FABP5 and PPARβ/δ, activates PPARβ/δ target genes and suppresses the LPS-induced inflammation in macrophages. Our study reveals a molecular mechanism through which FABP5 S-glutathionylation regulates macrophage inflammation in the pathogenesis of acute lung injury.

scholarly journals The protective role of Prolyl oligopeptidase (POP) inhibition in acute lung injury induced by intestinal ischemia-reperfusion

Oncotarget ◽  
2021 ◽  
Vol 12 (17) ◽  
pp. 1663-1676
Author(s):  
Giovanna Casili ◽  
Sarah Adriana Scuderi ◽  
Marika Lanza ◽  
Alessia Filippone ◽  
Rossella Basilotta ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Intestinal Ischemia ◽  
Ischemia Reperfusion ◽  
Protective Role ◽  
Prolyl Oligopeptidase ◽  
Intestinal Ischemia Reperfusion
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