scholarly journals Effect of Pretreated Colza Straw on the Growth and Extracellular Ligninolytic Enzymes Production by Lentinula edodes and Ganoderma lucidum

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 157
Author(s):  
Galina Makarenkova ◽  
Valda Balode ◽  
Dzintra Zala ◽  
Elina Azena ◽  
Alexander Rapoport ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Laccase Activity ◽  
Lentinula Edodes ◽  
Culture Media ◽  
Agricultural Waste ◽  
Ligninolytic Enzymes ◽  
Ligninolytic Enzyme ◽  
Solid Media ◽  
Extracellular Laccase ◽  
Surface Liquid

Lentinula edodes 3565 and Ganoderma lucidum 9621 were compared for their ability to produce lignocellulolytic enzymes in submerged (SM) and surface liquid (SL) fermentation of hydrolysed colza straw lignin waste that remained after the production of furfural and bioethanol (CS lignin). Application of cultivated mushrooms to dispose of pretreated colza straw agricultural waste is an approach to decrease the quantity of residual lignin while simultaneously obtaining active substances, e.g., the ligninolytic enzyme complex from mycelium. The effect of adding CS lignin to culture media on the yield of L. edodes and G. lucidum mycelium and extracellular laccase activity was studied. It was revealed that the mycelial growth of G. lucidum on solid media was significantly improved by adding CS lignin. Laccase activity during SL cultivation of L. edodes on medium with CS lignin gradually increased over the experiment starting on day 21 and peaked at 520 U/mL on day 28. G. lucidum expressed the maximum laccase activity, 540 U/mL, during the first 14 days of mycelium SM cultivation. Extracellular laccase activity was enhanced about 35- to 40-fold at cultivation of L. edodes and about 10- to 15-fold in the case of G. lucidum by supplementing liquid culture media with CS lignin.

scholarly journals Effect of medium pH and cultivation period on mycelial biomass, polysaccharide, and ligninolytic enzyme production by Ganoderma lucidum from Montenegro

2006 ◽  
Vol 58 (3) ◽  
pp. 179-182 ◽  
Author(s):  
Jelena Vukojevic ◽  
Mirjana Stajic ◽  
Sonja Duletic-Lausevic ◽  
Jasmina Simonic
Keyword(s):  
Ganoderma Lucidum ◽  
Enzyme Production ◽  
Ligninolytic Enzymes ◽  
Extracellular Polysaccharide ◽  
Ligninolytic Enzyme ◽  
Extracellular Polysaccharides ◽  
Medium Ph ◽  
Polysaccharide Production ◽  
Intracellular Polysaccharide ◽  
Maximal Production

The effect of initial medium pH on biomass, extracellular and intracellular polysaccharide, and ligninolytic enzyme production by Ganoderma lucidum was investigated at different pH values after 7 and 14 days of cultivation. Maximal production of biomass was recorded at pH 4.5 and 5.0; maximal production of extracellular polysaccharides at pH 7.0 and 3.0; and maximal production of intracellular polysaccharides at pH 7.0 and 5.5. Ligninolytic enzymes were not produced at any pH of the medium. Maximal biomass production was obtained on the 11th day of cultivation; maximal extracellular polysaccharide production on the 7th day; and maximal intracellular polysaccharide production on the 6th and 10th day of cultivation. .

Effective Management of Agro-Industrial Residues as Composting in Mushroom Industry and Utilization of Spent Mushroom Substrate for Bioremediation

2020 ◽  
pp. 1217-1236
Author(s):  
Rajender Singh ◽  
Mamta Chauhan
Keyword(s):  
Textile Industry ◽  
Ganoderma Lucidum ◽  
Lentinula Edodes ◽  
Ligninolytic Enzymes ◽  
Textile Wastewater ◽  
Industrial Scale ◽  
Spent Mushroom Substrate ◽  
Edible Fungi ◽  
Dyeing Process ◽  
Calocybe Indica

Different types of edible mushrooms like Agaricus, bisporus, A. bitoriqus, Pleurotus spp., Volvariella volvacea, Lentinula edodes, Calocybe indica, Flamullina, Ganoderma lucidum etc. are cultivated in industrial scale. Majority of edible fungi secretes extracellular Ligninocellulolytic enzymes like Laccase, lignin peroxidase, manganese peroxidase, cellulase etc. for effective conversion of ligninocellulolytic substrate to compositing form which led to fruiting of mushrooms. Consequently, an adequate disposal method is needed for the high quantities of spent mushroom substrate (SMS) generated in this agro-food industrial activity. On the other side, textile industry among the largest water consuming industries in the world and approximately, 10,000 different dyes and pigments are used at industrial scale. It is estimated that nearly 40% of the total dyes used in the dyeing process may find their way in wastewater. So, there is an attempt to utilize the ligninolytic enzymes rich SMS of different mushroom for efficiently biodegradation of textile wastewater & polyaromatic pollutants.

Polar vineyard pruning extracts increase the activity of the main ligninolytic enzymes in Lentinula edodes cultures

2007 ◽  
Vol 53 (10) ◽  
pp. 1150-1157 ◽  
Author(s):  
Citlalli Harris-Valle ◽  
Martín Esqueda ◽  
Alfonso Sánchez ◽  
Miguel Beltrán-García ◽  
Elisa M. Valenzuela-Soto
Keyword(s):  
Enzyme Activity ◽  
Biomass Production ◽  
Lentinula Edodes ◽  
Activity Patterns ◽  
Alcohol Oxidase ◽  
Ligninolytic Enzymes ◽  
Ligninolytic Enzyme ◽  
Dry Mass ◽  
Polar Extracts ◽  
The Relationship

Lentinula edodes is considered an alternative recycling agent for agricultural wastes, and there have been several studies to understand the relationship between its growth and ligninolytic activity. We tested the effect of wood from viticulture pruning, extracted with solvents of differing polarity, on the biomass production and activity pattern of ligninolytic enzymes. The analysis was done by measuring the mycelial dry mass and enzyme activity of liquid growth medium during the culture of L. edodes, adding either single extracts or a combination of extracts. Polar extracts enhanced mycelial production, and the activity patterns of lignin peroxidase, manganese peroxidase, aryl alcohol oxidase, and laccase were comparable to their activities predicted by ligninolysis models proposed for other fungi. We conclude that the polar extracts could be useful for enhancing fungal biomass production and for modifying lignin degradation because the regulation of ligninolytic enzyme activity is differentially influenced by the polarity of the extract.

Effective Management of Agro-Industrial Residues as Composting in Mushroom Industry and Utilization of Spent Mushroom Substrate for Bioremediation

2016 ◽  
pp. 158-177
Author(s):  
Rajender Singh ◽  
Mamta Chauhan
Keyword(s):  
Textile Industry ◽  
Ganoderma Lucidum ◽  
Lentinula Edodes ◽  
Ligninolytic Enzymes ◽  
Textile Wastewater ◽  
Industrial Scale ◽  
Spent Mushroom Substrate ◽  
Edible Fungi ◽  
Dyeing Process ◽  
Calocybe Indica

Different types of edible mushrooms like Agaricus, bisporus, A. bitoriqus, Pleurotus spp., Volvariella volvacea, Lentinula edodes, Calocybe indica, Flamullina, Ganoderma lucidum etc. are cultivated in industrial scale. Majority of edible fungi secretes extracellular Ligninocellulolytic enzymes like Laccase, lignin peroxidase, manganese peroxidase, cellulase etc. for effective conversion of ligninocellulolytic substrate to compositing form which led to fruiting of mushrooms. Consequently, an adequate disposal method is needed for the high quantities of spent mushroom substrate (SMS) generated in this agro-food industrial activity. On the other side, textile industry among the largest water consuming industries in the world and approximately, 10,000 different dyes and pigments are used at industrial scale. It is estimated that nearly 40% of the total dyes used in the dyeing process may find their way in wastewater. So, there is an attempt to utilize the ligninolytic enzymes rich SMS of different mushroom for efficiently biodegradation of textile wastewater & polyaromatic pollutants.

Tannic acid induces transcription of laccase genecglcc1in the white-rot fungusCoriolopsis gallica

2002 ◽  
Vol 48 (12) ◽  
pp. 1041-1047 ◽  
Author(s):  
José M Carbajo ◽  
Howard Junca ◽  
María C Terrón ◽  
Tania González ◽  
Susana Yagüe ◽  
...  
Keyword(s):  
Tannic Acid ◽  
Laccase Activity ◽  
Culture Media ◽  
Environmental Pollutants ◽  
White Rot Fungi ◽  
Extracellular Enzyme ◽  
White Rot ◽  
Laccase Gene ◽  
Extracellular Laccase

Laccase, a phenoloxidase enzyme secreted by white-rot fungi, has a significant role in the degradation of lignin and environmental pollutants. Coriolopsis gallica is a ligninolytic basidiomycete that produces high levels of this extracellular enzyme. A laccase gene cglcc1 from this fungus has been cloned and sequenced. The capacity of C. gallica to efficiently degrade polyphenols has been successfully applied in our laboratory to the biotreatment and decolorization of several industrial wastewaters. This study focused on the effect of tannic acid, a natural compound widely distributed in plants, on the production of laccase activity by C. gallica. Our results showed an evident increase of extracellular laccase levels when C. gallica was grown in the presence of tannic acid. Concentrations of 50 and 100 μM of this compound increased laccase activity when compared with control samples grown without tannic acid. In addition, we found an increase in laccase transcript levels in C. gallica grown in culture media supplemented with tannic acid. The role of tannic acid was shown to be an inductor of laccase activity in this fungus, due to the enhancement of expression of the laccase gene at the transcriptional level.Key words: laccase, tannic acid, Coriolopsis gallica, induction, gene transcription.

Production of Basidiomata and Ligninolytic Enzymes by the Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), in Licuri (Syagrus coronata) Wastes in Brazil

2016 ◽  
Vol 18 (12) ◽  
pp. 1141-1149 ◽  
Author(s):  
Thais Almeida de Menezes ◽  
Aline Simoes da Rocha Bispo ◽  
Maria Gabriela Bello Koblitz ◽  
Luciana Porto de Souza Vandenberghe ◽  
Helio Mitoshi Kamida ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Ligninolytic Enzymes ◽  
Medicinal Mushroom

Purification and Characterization of Laccase fromChaetomium thermophilium and Its Role in Humification

1998 ◽  
Vol 64 (9) ◽  
pp. 3175-3179 ◽  
Author(s):  
Benny Chefetz ◽  
Yona Chen ◽  
Yitzhak Hadar
Keyword(s):  
Municipal Solid Waste ◽  
Solid Waste ◽  
Laccase Activity ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Water Soluble ◽  
Type I ◽  
Light Spectrum ◽  
Solid Media ◽  
Wide Range

ABSTRACT Chaetomium thermophilium was isolated from composting municipal solid waste during the thermophilic stage of the process.C. thermophilium, a cellulolytic fungus, exhibited laccase activity when it was grown at 45°C both in solid media and in liquid media. Laccase activity reached a peak after 24 h in liquid shake culture. Laccase was purified by ultrafiltration, anion-exchange chromatography, and affinity chromatography. The purified enzyme was identified as a glycoprotein with a molecular mass of 77 kDa and an isoelectric point of 5.1. The laccase was stable for 1 h at 70°C and had half-lives of 24 and 12 h at 40 and 50°C, respectively. The enzyme was stable at pH 5 to 10, and the optimum pH for enzyme activity was 6. The purified laccase efficiently catalyzed a wide range of phenolic substrates but not tyrosine. The highest levels of affinity were the levels of affinity to syringaldazine and hydroxyquinone. The UV-visible light spectrum of the purified laccase had a peak at 604 nm (i.e., Cu type I), and the activity was strongly inhibited by Cu-chelating agents. When the hydrophobic acid fraction (the humic fraction of the water-soluble organic matter obtained from municipal solid waste compost) was added to a reaction assay mixture containing laccase and guaiacol, polymerization took place and a soluble polymer was formed. C. thermophilium laccase, which is produced during the thermophilic stage of composting, can remain active for a long period of time at high temperatures and alkaline pH values, and we suggest that this enzyme is involved in the humification process during composting.

scholarly journals Effect of Lignin-Containing Media on Growth of Medicinal Mushroom Lentinula Edodes

2017 ◽  
Vol 71 (1-2) ◽  
pp. 38-42
Author(s):  
Nataļja Matjuškova ◽  
Laura Okmane ◽  
Dzintra Zaļā ◽  
Linda Rozenfelde ◽  
Māris Puķe ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Nutrient Medium ◽  
Laccase Activity ◽  
Biomass Yield ◽  
Lentinula Edodes ◽  
Reference Conditions ◽  
Kraft Lignin ◽  
Mushroom Cultivation ◽  
Cultivation Medium ◽  
Solid Nutrient Medium

Abstract The effect of lignocellulose and lignin on growth of mycelium of mushroom Lentinula edodes and laccase activity in cultivation medium was studied. It was shown that cultivation of L. edodes mycelium in liquid nutrient medium with addition of 0.25-0.5% of kraft lignin increased mycelium biomass yield approximately two times compared with reference conditions without addition of lignin. Similar results were obtained in experiments in which 0.5% lignocellulose that remained after obtaining furfural, and 0.5% lignin that remained after obtaining furfural and glucose from wheat straw, were added to the nutrient medium. This effect was greater in the conditions of cultivation with good aeration, compared with static culture. Laccase activity in medium increased after addition of wheat straw lignocellulose or lignin only in the case of mycelium cultivation with aeration. In the case of mushroom cultivation on solid nutrient medium, addition of wheat straw lignocellulose and lignin promoted growth of mycelium only during the first 7 days of cultivation.

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