scholarly journals CLONING AND EXPRESSION OF A MURE LIGASE ENZYME AS A POTENTIAL TARGET AGAINST BACTERIA XANTHOMONAS ORYZAE PV. ORYZAE

2021 ◽  
Vol 44 (02) ◽  
Author(s):  
THI-HUYEN TRAN ◽  
NGOC-TUAN NGUYEN ◽  
LIN-WOO KANG
Keyword(s):  
Bacterial Blight ◽  
Xanthomonas Oryzae ◽  
Cloning And Expression ◽  
Diaminopimelic Acid ◽  
Uridine Diphosphate ◽  
Atp Binding Site ◽  
Tev Protease ◽  
Popular Interest ◽  
Hydrolysis Of ◽  
Sequence Identities

Xanthomonas oryzae pv. oryzae (Xoo) is causal agent of bacterial blight (BB) in rice. Many genes in Xoo have been identified in recently years. One of these genes, a gene coded for uridine diphosphate (UDP)-MurNAc-tripeptide ligase (MurE), catalyses the addition of meso-diaminopimelic acid (m-DAP) into peptidoglycan by coupled to the hydrolysis of ATP has more popular interest. However, there are no experimental data to confirm hypothesis of this enzyme in Xoo. A significant overview at the ATP binding site of most the MurE ligases demonstrated much more variable with amino acid sequence identities in this part, variable percentage around 22 to 26%. Besides, a refined homology structural feature between EcMurE and XooMurE will very important for determining possible involvement of the MurE ligase activity in Xoo. Therefore, a new recombinant protein named XooMurE from Xoo was purified with the N-terminal His-tagged form through a Ni-NTA column in this study. After purification, the Histag was removed then out of the N-terminal His-tagged XooMurE by TEV protease. Purification effectiveness of XooMurE over 95% in this study could produce an essential material for e studies about mechanism of XooMurE and consequently available direction for discovering novel anti-bacterial compounds against Xanthomonas oryzae pv. oryzae (xoo).  

Identification and Molecular Mapping of Xa32(t), a Novel Resistance Gene for Bacterial Blight (Xanthomonas oryzae pv. oryzae) in Rice

2009 ◽  
Vol 35 (7) ◽  
pp. 1173-1180 ◽  
Author(s):  
Chong-Ke ZHENG ◽  
Chun-Lian WANG ◽  
Yuan-Jie YU ◽  
Yun-Tao LIANG ◽  
Kai-Jun ZHAO
Keyword(s):  
Resistance Gene ◽  
Bacterial Blight ◽  
Molecular Mapping ◽  
Xanthomonas Oryzae

STRUCTURE OF THE EXTRACELLULAR POLYSACCHARIDE PRODUCED BY XANTHOMONAS ORYZAE

1962 ◽  
Vol 40 (12) ◽  
pp. 2204-2213 ◽  
Author(s):  
A. Misaki ◽  
S. Kirkwood ◽  
J. V. Scaletti ◽  
F. Smith
Keyword(s):  
Acid Hydrolysis ◽  
Glucuronic Acid ◽  
Periodate Oxidation ◽  
Extracellular Polysaccharide ◽  
Xanthomonas Oryzae ◽  
Sodium Borohydride Reduction ◽  
Glycoside Hydrolysis ◽  
Hydrolysis Of ◽  
Structural Significance ◽  
Molecular Proportion

The extracellular polysaccharide isolated from cultures of Xanthomonas oryzae is composed of D-glucose (5 molecular proportions), D-glucuronic acid (2 molecular proportions), and D-mannose (5 molecular proportions). Acid hydrolysis of this polysaccharide, which contains 0.3% combined pyruvic acid, yields 2-O-β-D-glucopyranosyluronic acid D-mannose, which has been characterized as its crystalline fully methylated β-glycoside. Hydrolysis of the methylated polysaccharide gives 2,3,4,6-tetra-O-methyl-D-mannose (3 molecular proportions), 2,3,4-tri-O-methyl-D-glucuronic acid (1 molecular proportion), 2,3,6-tri-O-methyl-D-glucose (4 molecular proportions), 3,4,6-tri-O-methyl-D-mannose (2 molecular proportions), 2,6-di-O-methyl-D-glucose (3 molecular proportions), 2,3-di-O-methyl-D-glucose (1 molecular proportion). The polyalcohol derived from the polysaccharide by periodate oxidation followed by sodium borohydride reduction gives upon acid hydrolysis glycerol (2 molecular proportions), erythritol (1 molecular proportion), and D-glucose (1 molecular proportion). The general structural significance of these findings is discussed.

scholarly journals Multiple Adhesin-Like Functions of Xanthomonas oryzae pv. oryzae Are Involved in Promoting Leaf Attachment, Entry, and Virulence on Rice

2009 ◽  
Vol 22 (1) ◽  
pp. 73-85 ◽  
Author(s):  
Amit Das ◽  
Nandini Rangaraj ◽  
Ramesh V. Sonti
Keyword(s):  
Bacterial Adhesion ◽  
Bacterial Blight ◽  
Fluorescent Protein ◽  
Protein A ◽  
Disease Process ◽  
Xanthomonas Oryzae ◽  
Type Iv ◽  
Genes Encoding ◽  
Green Fluorescent

Xanthomonas oryzae pv. oryzae is the causal agent of bacterial blight of rice. We have used enhanced green fluorescent protein-tagged X. oryzae pv. oryzae cells in conjunction with confocal microscopy to monitor the role of several adhesin-like functions in bacterial adhesion to leaf surface and early stages of leaf entry. Mutations in genes encoding either the Xanthomonas adhesin-like protein A (XadA) or its paralog, Xanthomonas adhesin-like protein B (XadB), as well as the X. oryzae pv. oryzae homolog of Yersinia autotransporter-like protein H (YapH), exhibit deficiencies in leaf attachment or entry. A mutation in the X. oryzae pv. oryzae pilQ gene, which is predicted to encode the type IV pilus secretin, appears to have no effect on leaf attachment or entry. The xadA– mutant is deficient in the ability to cause disease following surface inoculation while the XadB, YapH, and PilQ functions are less important than XadA for this process. The xadA– and xadB– mutants have no effect on virulence following wound inoculation whereas the yapH– and pilQ– mutants are always virulence deficient following wound inoculation. Overall, these results indicate that multiple adhesin-like functions are involved in promoting virulence of X. oryzae pv. oryzae, with preferential involvement of individual functions at different stages of the disease process.

scholarly journals The Role of a Host-Induced Arginase of Xanthomonas oryzae pv. oryzae in Promoting Virulence on Rice

2019 ◽  
Vol 109 (11) ◽  
pp. 1869-1877
Author(s):  
Yuqiang Zhang ◽  
Guichun Wu ◽  
Ian Palmer ◽  
Bo Wang ◽  
Guoliang Qian ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Bacterial Blight ◽  
Wild Type Strain ◽  
Bacterial Pathogen ◽  
Extracellular Polysaccharide ◽  
Rice Cultivar ◽  
Xanthomonas Oryzae ◽  
Wild Type ◽  
Bacterial Blight Of Rice

The plant bacterial pathogen Xanthomonas oryzae pv. oryzae causes bacterial blight of rice, which is one of the most destructive rice diseases prevalent in Asia and parts of Africa. Despite many years of research, how X. oryzae pv. oryzae causes bacterial blight of rice is still not completely understood. Here, we show that the loss of the rocF gene caused a significant decrease in the virulence of X. oryzae pv. oryzae in the susceptible rice cultivar IR24. Bioinformatics analysis demonstrated that rocF encodes arginase. Quantitative real-time PCR and Western blot assays revealed that rocF expression was significantly induced by rice and arginine. The rocF deletion mutant strain showed elevated sensitivity to hydrogen peroxide, reduced extracellular polysaccharide (EPS) production, and reduced biofilm formation, all of which are important determinants for the full virulence of X. oryzae pv. oryzae, compared with the wild-type strain. Taken together, the results of this study revealed a mechanism by which a bacterial arginase is required for the full virulence of X. oryzae pv. oryzae on rice because of its contribution to tolerance to reactive oxygen species, EPS production, and biofilm formation.

scholarly journals Transcriptional Modulation of Resistance against Xanthomonas oryzae pv. oryzae Korean Race K2 in japonica Rice

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 960
Author(s):  
Marjohn C. Niño ◽  
Yong-Gu Cho
Keyword(s):  
Bacterial Blight ◽  
Cellular Reprogramming ◽  
Xanthomonas Oryzae ◽  
Common Disease ◽  
Post Inoculation ◽  
Japonica Rice ◽  
Defense Compounds ◽  
Twin Arginine Translocation ◽  
Genes Encoding ◽  
Highly Expressed Genes

Bacterial blight is a common disease found in the rice-growing regions in the Korean peninsula. Identification of the gene network involved against Xanthomonas oryzae pv. oryzae Korean race K2 in popular japonica cultivars is essential in underpinning the molecular mechanism of resistance. A microarray of two popular Korean japonica rice cultivars, a bacterial blight susceptible Dongjin and resistant Jinbaek, was performed to investigate the transcripts of inducible genes at 48 h post-inoculation. A total of 771 differentially expressed genes were identified in Jinbaek, whereas 298 were found in Dongjin. The resistance observed in Jinbaek is likely participated by genes with predicted functions in transmembrane perception, intracellular signal transduction, and transcription activity. Moreover, the remarkable involvement of numerous WRKY proteins signifies orchestration of defense signals via robust cellular reprogramming, which leads to resistance. To discover genes essential to bacterial blight resistance in Jinbaek, 13 highly expressed genes encoding different protein classes were cloned and overexpressed in rice. Although none of the overexpression plants exhibited resistance comparable to Jinbaek, four candidate genes, including one twin-arginine translocation pathway signal (LOC_Os01g45640.1), one cytochrome p450 (LOC_Os09g10340.1), and two uncharacterized expressed protein (LOC_Os08g26230.4, LOC_Os09g04310.1) conferred partial resistance. However, of these four genes, only p450s have been reported to play an important role in the synthesis of plant defense compounds. These findings revealed the complexity of key immune signaling conduits critical to mounting a full defense against Xanthomonas. oryzae pv. oryzae race K2 in japonica rice.

Sign in / Sign up

Export Citation Format

Share Document