scholarly journals Duration of immunity acquired by vaccination with the live attenuated vaccine Avishield IB H120 against infectious bronchitis virus in SPF chickens

2020 ◽  
Vol 51 (6) ◽  
pp. 621-626
Author(s):  
Lana Ljuma Skupnjak ◽  
Anto Vrdoljak ◽  
Nikol Očuršćak

The most effective means of controlling infectious bronchitis in poultry is by vaccination. Live attenuated vaccines based on the H120 strain (Mass serotype) are the most commonly used vaccines. Since vaccination is often performed in the hatchery, long duration of immunity is preferred. Avishield IB H120, a live attenuated vaccine based on the strain H120 is registered across the European Union and other countries, and has a declared immunity period of up to 5 weeks after vaccination. This study presents the results of an additional survey demonstrating protection against challenge with the IBV strain M41 up to 8 weeks after a single vaccination with Avishield IB H120. Seven weeks after vaccination, 94% of chickens vaccinated by spray were protected against the challenge. Eight weeks after vaccination, 80% of spray vaccinated chickens and 75% of orally vaccinated chickens were still protected against the challenge with virulent IBV.

2011 ◽  
Vol 51 (3) ◽  
pp. 193-201
Author(s):  
Kang-Seuk Choi ◽  
Woo-Jin Jeon ◽  
Eun-Kyoung Lee ◽  
Soo-Jeong Kye ◽  
Mi-Ja Park ◽  
...  

Author(s):  
Katrin Auel

The role and position of national parliaments in European Union (EU) affairs have undergone a long, slow, and sometimes rocky, but overall rather remarkable, development. Long regarded as the victims of the integration process, they have continuously strengthened their institutional prerogatives and have become more actively involved in EU affairs. Since the Lisbon Treaty, national parliaments even have a formal and direct role in the European legislative process, namely, as guardians of the EU’s subsidiarity principle via the so-called early warning system. To what extent institutional provisions at the national or the European level provide national parliaments with effective means of influencing EU politics is still a largely open question. On the one hand, national parliaments still differ with regard to their institutional prerogatives and actual engagement in EU politics. On the other hand, the complex decision-making system of the EU, with its multitude of actors involved, makes it difficult to trace outcomes back to the influence of specific actors. Yet it is precisely this opacity of the EU policymaking process that has led to an emphasis on the parliamentary communication function and the way national parliaments can contribute to the democratic legitimacy of the EU by making EU political decisions and processes more accessible and transparent for the citizens. This deliberative aspect is also often emphasized in approaches to the role of national parliaments in the EU that challenge the territorially defined, standard account of parliamentary representation. Taking the multilevel character of the EU as well as the high degree of political and economic interdependence between the member states into account, parliamentary representation is conceptualized as extending beyond the nation-state and as shared across the EU, with a strong emphasis on the links between parliaments through inter-parliamentary cooperation and communication as well as on the representation of other member states’ citizens interests and concerns in parliamentary debates. Empirical research is still scarce, but existing studies provide evidence for the development of an increasingly dense web of formal and informal interactions between parliaments and for changes in the way national parliamentarians represent citizens in EU affairs.


2013 ◽  
Vol 22 (1) ◽  
pp. 59-89
Author(s):  
Simone Vezzani

As recognised by the International Law Commission in the 2011 Draft Articles on the Responsibility of International Organisations, the rule of the prior exhaustion of internal remedies also applies to cases where the international responsibility of international organisations is invoked, be it in the field of diplomatic protection or human rights. This essay focuses on the application of this rule to the European Union (EU). The author maintains that the legal remedies available to individuals alleging injury as a result of an internationally wrongful act of the EU include both direct remedies before EU courts and remedies before domestic tribunals. He then scrutinises whether each remedy is capable of providing individuals with accessible and effective means of redress.


Viruses ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 652
Author(s):  
Seung-Min Hong ◽  
Se-Hee An ◽  
Chung-Young Lee ◽  
Chang-Seon Song ◽  
Kang-Seuk Choi ◽  
...  

We established a cold-adapted infectious bronchitis virus (BP-caKII) by passaging a field virus through specific pathogen-free embryonated eggs 20 times at 32 °C. We characterized its growth kinetics and pathogenicity in embryonated eggs, and its tropism and persistence in different tissues from chickens; then, we evaluated pathogenicity by using a new premature reproductive tract pathogenicity model. Furthermore, we determined the complete genomic sequence of BP-caKII to understand the genetic changes related to cold adaptation. According to our results, BP-caKII clustered with the KII genotype viruses K2 and KM91, and showed less pathogenicity than K2, a live attenuated vaccine strain. BP-caKII showed delayed viremia, resulting in its delayed dissemination to the kidneys and cecal tonsils compared to K2 and KM91, the latter of which is a pathogenic field strain. A comparative genomics study revealed similar nucleotide sequences between BP-caKII, K2 and KM91 but clearly showed different mutations among them. BP-caKII shared several mutations with K2 (nsp13, 14, 15 and 16) following embryo adaptation but acquired multiple additional mutations in nonstructural proteins (nsp3, 4 and 12), spike proteins and nucleocapsid proteins following cold adaptation. Thus, the establishment of BP-caKII and the identified mutations in this study may provide insight into the genetic background of embryo and cold adaptations, and the attenuation of coronaviruses.


2020 ◽  
Vol 30 (2) ◽  
pp. 349-364
Author(s):  
Francisco Luque Janodet

The second decade of the 21st century has seen the emergence and establishment of a series of movements that have led to the rise of Eurosceptic parties in France and Germany and the departure of the United Kingdom from the European Union. Considering the current political context and given the lack of studies addressing this object of study, this paper analyses the role of metaphor and metonymy in Eurosceptic discourse in French and Spanish. To this end, once the theoretical fundamentals of this research has been submitted, the compiled textual corpus is presented. This corpus consists of documents, political programmes and manifestos drafted by a number of openly Eurosceptic political parties in France, Switzerland and Spain. From this corpus, the underlying conceptual metaphors have been analyzed, as well as the orientational metaphors and the documented metonymies from the metaphorical expressions used. This study concludes that metaphor is, in political discourse, an effective means of transmitting a certain ideology. Thus, the most used conceptual metaphors have been THE EXIT FROM THE EUROPEAN UNION IS A WAY, THE EXIT FROM THE EUROPEAN UNION IS A FIGHT and POLITICS ARE A FIGHT. It is also concluded that the use of metaphor is capable of changing the citizen’s perception and conception of European Community policies and the European Union.


2020 ◽  
Author(s):  
Jing Zhao ◽  
Lu Sun ◽  
Ye Zhao ◽  
Delan Feng ◽  
Jinlong Cheng ◽  
...  

Coronavirus (CoV) nsp15 is an endoribonuclease conserved throughout the CoV family. The enzymatic activity and crystal structure of infectious bronchitis virus (IBV) nsp15 are undefined, and the protein’s role in replication remains unclear. We verified the uridylate-specific endoribonuclease (EndoU) activity of IBV and found that the EndoU active sites were located in the C-terminus of nsp15 and included His223, His238, Lys278 and Tyr334. We further constructed an infectious clone of the IBV-rSD strain (rSD-wild-type [WT]) and EndoU-deficient IBVs by changing the codon for the EndoU catalytic residues to alanine. Both the rSD-WT and EndoU-deficient viruses propagated efficiently in embryonated chicken eggs. Conversely, EndoU-deficient viral propagation was severely impaired in chicken embryonic kidney cells, which was reflected in the lower viral mRNA accumulation and protein synthesis. After infecting chickens with the parental rSD-WT strain and EndoU-deficient viruses, the EndoU-deficient-virus-infected chickens presented reduced mortality, tissue injury and viral shedding. IMPORTANCE Coronaviruses can emerge from animal reservoirs into naive host species to cause pandemic respiratory and gastrointestinal diseases with significant mortality in humans and domestic animals. Infectious bronchitis virus (IBV), a γ-coronavirus, infects respiratory, renal and reproductive systems, causing millions of dollars in lost revenue worldwide annually. Mutating the viral endoribonuclease resulted in an attenuated virus and prevented protein kinase R activation. Therefore, EndoU activity is a virulence factor in IBV infections, thus providing an approach for generating live-attenuated vaccine candidates for emerging coronaviruses.


2007 ◽  
Vol 28 (6) ◽  
pp. 702-707 ◽  
Author(s):  
Mini Kamboj ◽  
Kent A. Sepkowitz

Objective.Persons who receive live attenuated vaccine may occasionally transmit the vaccine strain to others. The risk of such transmission is a concern, especially for persons who provide care to immunocompromised patients (ie, family and healthcare workers [HCWs]). Since the Advisory Committee on Immunization Practices released guidelines 10 years ago, several new live attenuated products have been introduced, and additional information on older vaccines has become available. To better define the risk of transmission associated with live vaccines, we reviewed the currently available literature.Results.A review of the medical literature revealed no major risk of transmission associated with any live attenuated vaccine. A theoretical risk continues to exist for the live attenuated intranasal influenza vaccine and the smallpox vaccine.Conclusions.The available data support routine vaccination with live attenuated vaccines for all household contacts of immunocompromised patients and for HCWs caring for such Patients. The benefit for immunocompromised patients of providing herd immunity against this group of potentially devastating pathogens outweighs the risk, if any, of secondary transmission.


2013 ◽  
Vol 162 (1) ◽  
pp. 53-61 ◽  
Author(s):  
Ying Shun Zhou ◽  
Yi Zhang ◽  
Hong Ning Wang ◽  
Wen Qiao Fan ◽  
Xin Yang ◽  
...  

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