Pathogenic variability of Pseudocercospora griseola isolates from two provinces of Ecuador

Common bean (Phaseolus vulgaris L.) is the most important edible grain legume in Ecuador. The Ministry of Agriculture, Cattle Raising, Aquaculture and Fisheries of this country estimated that 71,600 hectares were cultivated in 2012; of the sown area, 53% was dedicated to dry grain and the remaining 47% to fresh grain. Beans are an important source of income for farmers and food for thousands of Ecuadorian families. Crop performance and yield are affected by diseases such as angular leaf spot caused by Pseudocercospora griseola (Sacc.) Ferraris. The best alternative for disease control is to use resistant varieties. However, durability of field resistance will depend on the knowledge of angular leaf spot variability. In Ecuador, detailed and systematic studies on the variability of the angular leaf spot pathogen have not been conducted. For this reason, the pathogenic variability of 21 isolates of angular leaf spot, collected from the provinces of Carchi and Imbabura in the Ecuadorian Andes, was studied. Thirteen different pathotypes were identified, five of them showed compatibility with only the group of Andean differentials and the remaining eight showed compatibility with two groups of differentials (Andean and Mesoamerican). Race 63:0 was the most frequent and race 31:63 was the most virulent. None of the isolates were compatible with differential Cornell 49-242. Therefore, bean breeding lines in Ecuador would benefit by incorporating the Phg-2 resistance gene present in differential Cornell 49-242 .

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Pathotypes Characterization and Virulence Diversity of Pseudocercospora griseola the Causal Agent of Angular Leaf Spot Disease Collected from Major Common Bean (Phaseolus vulgaris L.) Growing Areas of Ethiopia

Journal of Plant Pathology & Microbiology ◽

10.4172/2157-7471.1000445 ◽

2018 ◽

Vol 09 (08) ◽

Cited By ~ 1

Author(s):

Yayis Rezene ◽

Kassahun Tesfaye ◽

Mukankusi Clare ◽

Paul Gepts

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Leaf Spot ◽

Causal Agent ◽

Angular Leaf Spot ◽

Leaf Spot Disease ◽

Phaseolus Vulgaris L ◽

Spot Disease ◽

Pseudocercospora Griseola ◽

Virulence Diversity

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Evaluation of Selected French Bean (Phaseolus vulgaris L.) Genotypes for Resistance to Angular Leaf Spot (Pseudocercospora griseola) in Western Kenya

American Journal of Experimental Agriculture ◽

10.9734/ajea/2016/26874 ◽

2016 ◽

Vol 13 (4) ◽

pp. 1-6

Author(s):

S Kimno ◽

O Kiplagat ◽

E Arunga ◽

E Chepkoech

Keyword(s):

Phaseolus Vulgaris ◽

Leaf Spot ◽

French Bean ◽

Angular Leaf Spot ◽

Phaseolus Vulgaris L ◽

Western Kenya ◽

Pseudocercospora Griseola

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Identification of resistance sources of common bean (Phaseolus vulgaris L.) to angular leaf spot (Pseudocercospora griseola)

Revista Facultad Nacional de Agronomía Medellín ◽

10.15446/rfnam.v72n2.70238 ◽

2019 ◽

Vol 72 (2) ◽

pp. 8785-8791

Author(s):

Diego Rodríguez ◽

James Beaver ◽

Consuelo Estévez de Jensen ◽

Tim Porch

Keyword(s):

Puerto Rico ◽

Phaseolus Vulgaris ◽

Common Bean ◽

Resistance Genes ◽

Leaf Spot ◽

Angular Leaf Spot ◽

Gene Pools ◽

Phaseolus Vulgaris L ◽

Breeding Programs ◽

Pseudocercospora Griseola

Common bean (Phaseolus vulgaris L.) is the most important edible legume in the world and is an important source of income for farmers and food for millions of families. Angular Leaf Spot (ALS), caused by the fungus Pseudocercospora griseola (Sacc.) Ferraris, is one of the most important diseases in the production of common bean with the potential to cause significant seed yield losses. An effective and environmentally friendly alternative to control this disease is the use of improved varieties that combine resistance genes of Andean and Mesoamerican origin. In this study, the response of 181 bean genotypes (coming from different breeding programs of several countries including the USA, Puerto Rico, Honduras, Ecuador, Colombia, Tanzania, Malawi, and Angola) to two angular spot isolates (races 61:11 and 63:51, prevalent in Isabela and Juana Díaz, Puerto Rico) were evaluated. Many of these genotypes contained resistant genes to different biotic and to abiotic stress. A total of 16 lines were identified to have resistance to both races. The resistant lines include the Andean breeding lines CAL 143, 277 and the Mesoamerican cultivar ‘Ouro Negro’ that possess the resistance genes Phg-5, Phg-1, Phg-3, respectively. This information can help common bean breeding programs to pyramid genes from the Andean and Mesoamerican gene pools to generate varieties with long-lasting resistance to this disease.

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Response of Ugandan common bean varieties to Pseudocercospora griseola and Angular leaf spot disease development in varietal mixtures

International Journal of Pest Management ◽

10.1080/09670874.2016.1234656 ◽

2016 ◽

Vol 63 (2) ◽

pp. 119-127 ◽

Cited By ~ 3

Author(s):

N. Olango ◽

G. Tusiime ◽

J.W. Mulumba ◽

R. Nankya ◽

C. Fadda ◽

...

Keyword(s):

Common Bean ◽

Leaf Spot ◽

Angular Leaf Spot ◽

Leaf Spot Disease ◽

Disease Development ◽

Spot Disease ◽

Pseudocercospora Griseola

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Characterization of the common bean host and Pseudocercospora griseola, the causative agent of angular leaf spot disease in Tanzania

African Journal of Plant Science ◽

10.5897/ajps2016.1427 ◽

2016 ◽

Vol 10 (11) ◽

pp. 238-245 ◽

Cited By ~ 1

Author(s):

Amos Chilagane Luseko ◽

Nchimbi-Msolla Susan ◽

Mbogo Kusolwa Paul ◽

Gabriel Porch Timothy ◽

Miryam Serrato Diaz Luz ◽

...

Keyword(s):

Common Bean ◽

Causative Agent ◽

Leaf Spot ◽

Angular Leaf Spot ◽

Leaf Spot Disease ◽

Spot Disease ◽

The Common ◽

Pseudocercospora Griseola

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Identification of Phaeoisariopsis griseola pathotypes from five States in Brazil

Fitopatologia Brasileira ◽

10.1590/s0100-41582002000100012 ◽

2002 ◽

Vol 27 (1) ◽

pp. 78-81 ◽

Cited By ~ 19

Author(s):

ALOISIO SARTORATO

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Resistance Genes ◽

Leaf Spot ◽

Angular Leaf Spot ◽

Phaeoisariopsis Griseola ◽

Moist Chamber ◽

Differential Cultivars ◽

Pathogenic Variability ◽

Resistant Genotypes

Due to the increased importance of angular leaf spot of common bean (Phaseolus vulgaris) in Brazil, monitoring the pathogenic variability of its causal agent (Phaeoisariopsis griseola) is the best strategy for a breeding program aimed at developing resistant genotypes. Fifty one isolates of P. griseola collected in five Brazilian States were tested on a set of 12 international differential cultivars in the greenhouse. When inoculated plants showed symptoms but no sporulation was observed, they were transferred to a moist chamber for approximately 20-24 h. After this period of time, if no sporulation was observed, the plants were considered resistant; otherwise, they were considered susceptible. From the fifty-one tested isolates, seven different pathotypes were identified. No Andean pathotypes were identified; consequently, all isolates were classified as Middle American pathotypes. Pathotype 63-31 was the most widespread. Pathotype 63-63 overcame resistance genes present in all differential cultivars and also the resistance gene(s) present in the cultivar AND 277. This fact has important implications for breeding angular leaf spot resistance in beans, and suggests that searching for new resistance genes to angular leaf spot must be pursued.

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