scholarly journals EFFECT OF SACCHAROMYCES CEREVISIAE FEED SUPPLEMENTATION ON HAEMATOLOGY AND REPRODUCTIVE PARAMETERS FOR ALGERIAN RABBITS

2021 ◽  
Vol 186 (2) ◽  
pp. 111-122
Author(s):  
Omar BESSEBOUA ◽  
Abdelhanine AYAD
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sperm Concentration ◽  
Feed Additives ◽  
Semen Parameters ◽  
Control Group ◽  
Head Group ◽  
Corpuscular Haemoglobin ◽  
Sperm Analysis ◽  
Positive Effects ◽  
Mean Corpuscular Haemoglobin

This study aims at investigating the effect of Saccharomyces cerevisiae (SC) supplementation on reproductive performance, haematological parameters and fertility of rabbits under Algerian conditions. The animals were divided into three groups and received the same feed ration during the experimental period. The control group received a basal diet without feed additives (Group#0) and the two yeast SC groups received 0.3 and 0.6 g/day per head (Group#1 and Group#2, respectively). Semen and blood samples were collected for determination of semen parameters and haematology. The weights of rabbits treated with SC 0.3 g/day were statistically significantly different (P< 0.05) from the control groups and group treated with SC 0.6 g/day. There were significant differences between the treatment groups for (RBCs), haemoglobin (HGB), haematocrit (HCT) and mean corpuscular haemoglobin (MCH) values, with higher values in rabbits supplemented with SC 0.3 g/day and 0.6 g/day, compared to those in the control group. The scrotal diameter did not differ between the dietary treatments. When compared with the control group, feeding rabbits graded levels of SC resulted in an increase in the average semen volume, mass motility and individual motility at day 51 of the experiment. On the other hand, the sperm concentration was significantly lower (P< 0.05) in rabbits supplemented with SC 0.3 g/day and 0.6 g/day during the two months compared to that in the control group. The spermatozoa mortality rate was lower for the rabbits supplemented with SC 0.3 g/day and 0.6 g/day (15.7% and 11.4%, respectively), compared to that in the control group (24%). In conclusion, this study has shown that inclusion of SC 0.3 g/day and 0.6 g/day in the diets of rabbit has positive effects on body weight and sperm analysis. Moreover, it increases the level (RBCs), haemoglobin (HGB), haematocrit (HCT) and mean corpuscular haemoglobin (MCH).

scholarly journals Curcumin Along With Fe3O4 Nanoparticles Improved Sperm Parameters In Rats With Testicular Ischemia

2021 ◽  
Vol 10 ◽  
pp. e2034
Author(s):  
Shabnam Zarei Moradi ◽  
Seyed Abdolhamid Angaji ◽  
Mitra Salehi ◽  
Mehrdad Hashemi
Keyword(s):  
Fe3o4 Nanoparticles ◽  
Sperm Concentration ◽  
Concomitant Administration ◽  
Testicular Tissue ◽  
Sperm Parameters ◽  
Male Rats ◽  
Semen Parameters ◽  
Control Group ◽  
Fe3o4 Nps ◽  
Testicular Ischemia

Background: Ischemic/reperfusion (I/R) in testicular tissue is one reason for the worldwide increase in male infertility. In the present study, we assessed the effects of curcumin and Fe3O4 nanoparticles (NPs) on sperm parameters in rats with I/R damage. Materials and Methods: Forty-eight adult male rats were divided into two groups (n=24 per group): control and torsion/detorsion. The control and torsion/detorsion groups were divided into four subgroups include sham, Fe3O4 NPs, curcumin, and Fe3O4 NPs+curcumin. After the rats were sacrificed, semen was collected from their epididymal tissues to assess sperm viability, motility, concentration, and morphology. Results: Curcumin significantly improved viability, motility, and normal sperm morphology in rats with I/R damage compared to the control group; however, it did not have a significant effect on sperm concentration (P<0.001). Fe3O4 NPs alone decreased all sperm parameters in the control and I/R rats (P<0.001). However, concomitant administration of Fe3O4 nanoparticles with curcumin significantly improved sperm parameters in rats with I/R damage (P<0.001). Conclusion: The increase in all semen parameters in the experimental groups with concomitant use of Fe3O4 NPs plus curcumin indicated that green synthesis of NPs could be recommended for future clinical studies.

scholarly journals Association between semen quality among men with different occupational exposures and risk of recurrent spontaneous abortion in island residents

2019 ◽  
Vol 02 (02) ◽  
pp. 49-57
Author(s):  
Wenli Cao ◽  
Xinyao Pan ◽  
Feijun Ye ◽  
Jing Zhou ◽  
Zengshu Huang ◽  
...  
Keyword(s):  
Spontaneous Abortion ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Occupational Exposures ◽  
Recurrent Spontaneous Abortion ◽  
Semen Parameters ◽  
Control Group ◽  
Effective Prevention ◽  
Mechanistic Investigation ◽  
Significant Difference

Objective: This study aimed to evaluate the semen quality of male adults whose spouses suffer from recurrent spontaneous abortion (RSA) in the island area, and to explore the association between RSA and occupational exposures. Methods: A total of 131 male patients were recruited and divided into two groups: spouse with recurrent spontaneous abortion group (RSA group, [Formula: see text]) and the normal fertility group (control group, [Formula: see text]). Information such as height, weight and occupational exposure history of 131 men were obtained. Semen samples were collected and analyzed. Differences in semen parameters and DNA fragmentation index (DFI) between the two groups were compared. Odds ratios (ORs) and their corresponding 95% confidence intervals were calculated to evaluate the association between occupational exposures and RSA status. Results: Overall, no significant difference was found in sperm concentration, progressive motility and normal morphology rate between RSA and control groups. Only DFI was observed to be significantly higher in the RSA group. Evaluation of the receiver operating characteristic (ROC) curve showed DFI (AUC: 0.623, [Formula: see text]) could discriminate between males from the couples with RSA and without RSA. Conclusion: The analysis of conventional semen parameters could not directly reflect their influence on embryonic development, and test of integrity of the sperm DNA is of paramount importance to fully understand male fertility. It is crucial to conduct studies regarding occupational exposures and pregnancy loss and/or RSA risk, since it will provide population-level data to aid in the identification of important risk factors that warrant further mechanistic investigation, and eventually lead to effective prevention and treatment strategies.

scholarly journals Effects of Astaxanthin on Miniature Pig Sperm Cryopreservation

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Eunjoo Lee ◽  
Daeyoung Kim
Keyword(s):  
Sperm Motility ◽  
Semen Parameters ◽  
Control Group ◽  
Computer Assisted ◽  
Miniature Pig ◽  
Oxidation Stress ◽  
Sperm Analysis ◽  
Semen Parameter ◽  
Boar Sperm ◽  
Sperm Plasma Membrane

The purpose of this study is to evaluate the effects of astaxanthin added to freezing buffer on semen parameters, total sperm oxidation stress after postthawing of boar sperm, and lipid peroxidation (LPO) which is caused by reactive oxygen species (ROS) in sperm membrane. Varying concentrations of astaxanthin (0, 10, 50, 100, and 500 μM) were used in the freezing buffer during cryopreservation to protect the DNA of thawed miniature pig sperm. Semen parameter was measured using computer-assisted sperm analysis (CASA) for sperm motility, and then ROS rate and oxidative stress of boar sperm were determined using fluorescence-activated cell sorting (FACS). Sperm motility was higher (p<0.05) in the astaxanthin group than in the control group. Sperm motility and the number of progressive motile sperm were higher (p<0.05) in the astaxanthin 500 μM group than in the control group. In ROS evaluation, the astaxanthin group had lower intracellular O2 and H2O2 in viable sperm. Yo-Pro-I/HE and PI/H2DCFDA staining as revealed using flow cytometry was lower in astaxanthin groups than in the other groups. As a result, we found that astaxanthin could protect the sperm plasma membrane from free radicals and LPO during boar sperm postthawing.

scholarly journals Performance, blood chemistry, carcass and economic evaluation of sheep on dietary supplements of cabbage and carrot wastes

2021 ◽  
Vol 12 (2) ◽  
pp. 574-586
Author(s):  
Michael Igbaekemen Okoruwa ◽  
Emmanuel Eromosele Ikheloa ◽  
Debola Olusegun Oshibanjo
Keyword(s):  
Economic Evaluation ◽  
Blood Cell ◽  
Blood Chemistry ◽  
Daily Weight Gain ◽  
Control Group ◽  
Corpuscular Haemoglobin ◽  
Significant Difference ◽  
Net Profit ◽  
Guinea Grass ◽  
Mean Corpuscular Haemoglobin

The study was conducted to determine the effect of cabbage and carrot wastes supplementation on performance, blood chemistry, carcass and economic evaluation of sheep. Twenty four West African dwarf male sheep of about 8 – 9 months old with average initial weight of 9.00 ± 0.94kg were randomly allotted to four dietary treatments with six sheep per treatment, in a completely randomized design. The sheep received 60% guinea grass and 40% concentrate diet for TD1 (control group), while 50% guinea grass and 40% concentrate diet with either 10% cabbage waste, 5% cabbage plus 5% carrot wastes or 10% carrot waste were for diets TD2, TD3 and TD4 respectively. The results showed that sheep on TD1 were significantly (p<0.05) higher in feed conversion ratio, creatinine, urea, rumen with intestine and total fixed cost. Diet TD2 was significantly (p<0.05) improved in daily weight gain, digestibility of dry matter, crude protein with fiber and nitrogen free extract, haemoglobin, packed cell volume, mean corpuscular haemoglobin, white blood cell, lymphocyte, neutrophil, total protein, albumin, glucose, dressing percentage, sale of sheep, total revenue and net profit. Red blood cell, cholesterol and triglyceride were increased significantly (p<0.05) in TD3, while daily feed intake, digestibility of ash, feed cost and total variable cost were better in TD4. Meanwhile, no significant difference (p>0.05) was found in initial body weight, ether extract digestibility, mean corpuscular volume, mean corpuscular haemoglobin concentration, monocyte, eosinophil, basophil, globulin and some relative organs weight among sheep on treatments. It can be concluded that inclusion level of 10% cabbage waste as supplement to 50% guinea grass and 40% concentrate diet (TD2) improved performance, health status and carcass indices of sheep with reduced cost that resulted in higher net profit.

scholarly journals Effect of Ivermectin on semen parameters and levels of Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) enzymes in seminal plasma of Iraqi Awassi rams

2014 ◽  
Vol 13 (1) ◽  
pp. 115
Author(s):  
Ali. J. Ali
Keyword(s):  
Seminal Plasma ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Semen Quality ◽  
Harmful Effect ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Control Group ◽  
Volume Percentage ◽  
Semen Collection

The objective of this study was to evaluate the effect of Ivermectin on semen quality and estimation of alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in seminal plasma. Six mature Awassi rams aged 2-3 years and weighing 38-42 Kg were used in this study. Semen collection was done weekly for five weeks from the same ram. The first two collection considered as training for the animal and discarded, the second two collection were considers for the control group. After administration of the drug (Ivermectin), semen samples were collected from the Awassi ram 1, 7,14 days. The parameter studies were included semen volume, percentage motility of sperm, sperm concentration, live sperm, and morphology percentage. Samples of seminal plasma were analyzed for the estimation of alanine aminotransferase (ALT), aspartate aminotransferase (AST). Results of this study indicate that Ivermectin leads to significant (P<0.05) decreased in semen parameters after the first semen collection. Reaction time significantly higher (P<0.05) in the treated group after 1 day of injection. Alanine aminotransferase ALT, Aspartate aminotransferase AST show no significant differences in activities in seminal plasma during the study. In conclusion: it is preferable to use the animals for the purpose of reproduction only after at least 7 days after Ivermectin injection due to it harmful effect on semen quality.

scholarly journals Haematological responses of the Indian major carp Labeo rohita to Saprolegniasis

2017 ◽  
Vol 64 (2) ◽  
Author(s):  
Chandan Debnath ◽  
Basanta Kumar Das ◽  
Lopamudra Sahoo
Keyword(s):  
Labeo Rohita ◽  
Haemoglobin Concentration ◽  
Infected Fish ◽  
Control Group ◽  
Total Leucocyte Count ◽  
Blood Profile ◽  
Corpuscular Haemoglobin ◽  
Indian Major Carps ◽  
Management Measures ◽  
Mean Corpuscular Haemoglobin

Saprolegniasis (cotton wool disease), due to infestation by Saprolegnia parasitica, is a major fungal disease in fish. Present study was conducted in the Indian major carps, rohu, Labeo rohita (Hamilton, 1822). Fingerlings of rohu (10.5±2.2 g) were artificially infected with the fungal parasite S. parasitica via injection (T1), cohabitation (T2) as well as immersion (T3) and compared with a control group (T0) with no infection. Blood samples were collected at 15 days interval over a period of 45 days and analysed for haematological parameters. Haemoglobin level in infected fish was lower (p≤0.05) than non-infected fish on 15, 30 and 45 days post-infection (dpi) except in cohabited fish on 45 dpi. Total erythrocyte count (TEC) in infected fish was lower (p≤0.05) than non-infected fish on 15, 30 and 45 dpi. Total leucocyte count (TLC) was higher (p≤0.05) in infected fish on 15 dpi; however no difference (p≥0.05) was noticed on 30 dpi. TLC in injected group was higher (p≤0.05) than control group on 45 dpi. Haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and leucocrit (Lct) values in infected group were lower (p≤0.05) than control group. There was no difference (p≥0.05) in mortality among the groups on 15 dpi; however, on 30 and 45 dpi, the group subjected to immersion exposure recorded highest mortality followed by injected and cohabitated groups. Varying degrees of histological alterations were observed in the tissues due to the fungal infestation. The results indicated that S. parasitica significantly altererd the blood profile of infected rohu leading to 45-50% mortality. Therefore appropriate management measures need to be adopted for preventing infestation by S. parasitica in carp culture systems for realising sustainable fish production.

scholarly journals Effect of feeding yeast culture on ruminal fermentation and blood indicators of Holstein dairy cows

2011 ◽  
Vol 80 (2) ◽  
pp. 139-145 ◽  
Author(s):  
Petr Doležal ◽  
Jan Dvořáček ◽  
Jan Doležal ◽  
Jana Čermáková ◽  
Ladislav Zeman ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dairy Cows ◽  
Volatile Fatty Acids ◽  
Control Group ◽  
Yeast Culture ◽  
Ruminal Fermentation ◽  
Positive Effects ◽  
Concentrate Mixture ◽  
Experimental Group ◽  
Holstein Dairy Cows

The experiment was conducted to evaluate the effect of yeast culture (Saccharomyces cerevisiae) supplementation on ruminal fermentation in 20 Holstein dairy cows divided into control and experimental groups, each group of 10 cows. The animals received a diet based on maize silage (19 kg), alfalfa silage (15 kg), meadow hay (1.5 kg), extracted rapeseed meal (1 kg) and concentrate mixture (9.5 kg). The diets were fed as a total mixed ration. The supplement of yeast culture Levucell® SC 20 (Saccharomyces cerevisiae – CNCM I-1077; min. content 2 × 1010 CFU·g-1) was added to the concentrate mixture in the ration fed to the experimental group of animals. The addition of yeast culture significantly (P < 0.01) increased ruminal pH but had no positive effects on the increased production of volatile fatty acids. The supplementation of yeast culture significantly (P < 0.01) increased numbers of protozoa in the rumen of dairy cows of the experimental group (361.3 ± 18.315) compared to the control group (308.3 ± 37.505). The addition of yeast culture significantly (P < 0.01) increased concentration of serum glucose, calcium, phosphorus, copper, zinc, magnesium and AST (P < 0.05). As compared to the control group (4.948 ± 0.0384 mmol·l-1), the level of urea in the blood serum was significantly decreased (P < 0.01) in the experimental group of cows. Our results show that the supplementation of Saccharomyces cerevisiae culture at recommended doses enhances ruminal fermentation which may have a positive effect on milk production and health status of Holstein dairy cows.

scholarly journals Resveratrol-Based Multivitamin Supplement Increases Sperm Concentration and Motility in Idiopathic Male Infertility: A Pilot Clinical Study

2020 ◽  
Vol 9 (12) ◽  
pp. 4017
Author(s):  
Ester Illiano ◽  
Francesco Trama ◽  
Alessandro Zucchi ◽  
Rossana Giulietta Iannitti ◽  
Bernard Fioretti ◽  
...  
Keyword(s):  
Clinical Study ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Mitochondrial Activity ◽  
Male Factor ◽  
Oral Tablet ◽  
Positive Effects ◽  
Idiopathic Infertility ◽  
Multivitamin Supplement

Background. It is known that a multitude of factors may lead to male factor infertility, but still, in the majority of cases, the cause remains largely idiopathic, reflecting poor understanding of the basic process of spermatogenesis and the mechanisms involved. Resveratrol is a polyphenol compound that displays several cellular aspects mainly associated with SIRT1-pathway activation and promotion of mitochondrial enhancer activities. In several animal models, resveratrol has shown positive effects on mitochondria and membrane potential. This could explain effects on sperm concentration and motility. The aim of this study is to evaluate the effects on the semen parameters of GENANTE®, a multivitamin supplement containing 150 mg of resveratrol/day, in patients with idiopathic infertility. Methods. This was a prospective single center clinical study. Twenty patients took a multivitamin supplement based on 150 mg of resveratrol (GENANTE®), in the form of an oral tablet every 12 h, and were followed up at 1, 3, and 6 months after treatment. Pre- and post-treatment evaluation included history, clinical examination, semen analysis, hormonal determinations, and scrotal and prostatic ultrasound. Results. Our preliminary pilot study demonstrated that the multivitamin supplement based on resveratrol improves sperm motility (48.3% ± 13.8 vs. 59.0% ± 12.8, p = 0.0001) and concentration (22.6 × 106/mL ± 9.5 vs. 25.7 × 106/mL ± 8.1, p = 0.0001) after 3 and 6 months of treatment in men with idiopathic infertility. Conclusion. Our data suggest that targeting the metabolic and energetic pathways involved in spermatogenesis and mitochondrial activity could lead to potential effects and counteract subfertility/infertility in men through a mitochondria dynamics mechanism. Trial registration number: ClinicalTrials.gov registration identifier: NCT03864198, registered on 1 January 2019.

scholarly journals Response to a Salmonella Typhimurium challenge in piglets supplemented with protected sodium butyrate or Bacillus licheniformis: effects on performance, intestinal health and behavior1,2

2017 ◽  
Vol 1 (2) ◽  
pp. 186-200 ◽  
Author(s):  
E. Barba-Vidal ◽  
V. F. B. Roll ◽  
L. Castillejos ◽  
A. A. Guerra-Ordaz ◽  
X. Manteca ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Behavior Analysis ◽  
Bacillus Licheniformis ◽  
Sodium Butyrate ◽  
Acute Phase Proteins ◽  
Feed Additives ◽  
Control Group ◽  
Post Inoculation ◽  
Salmonella Spp ◽  
Positive Effects

Abstract Salmonella spp. is one of the worldwide leading causes of food-borne illnesses for which the inclusion of probiotics or organic acids in animal feeds can be useful control methods. Experimental models are utilized to test the efficacy of strategies against pathogens, but they exhibit limitations which may preclude finding sensible evaluation parameters. The objective of this work is to evaluate the efficacy of 2 different feed additives; a Bacillus licheniformis based probiotic and a protected sodium butyrate (SB) salt, using an experimental model of salmonellosis and, second, to explore if behavior analysis can be used as a sensible evaluation tool for additives evaluation. A total of 78 piglets weaned at 24 d, 8.3 kg BW, were used. Seventy-two were placed in 3 rooms of 8 pens (3 animals/pen) with evenly distributed treatments (n = 8): CON, control group with plain diet; PRO, plain diet with 1 kg/t of Proporc (109 cfu of B. licheniformis/kg of feed), and BUT, plain diet with 3 kg/t of Gustor BP70 (2.1 g of partially protected SB salt/kg of feed). Remaining piglets (n = 6) were separated and used as a challenge negative control. The experiment lasted 16 d. After 1 wk of adaptation, animals were challenged with 5 × 108 cfu of Salmonella Typhimurium. One pig per pen was euthanized and sampled at d 4 and 8 post-inoculation (PI). There were no significant differences among treatments for ADFI, ADG, G:F, rectal temperature, fecal consistency, pH, ammonia, short-chain fatty acids and lactic acid concentrations, cytokine TNF-α, Pig-MAP acute-phase proteins and histological parameters. However, both products were equally able to reduce colonization and shedding of Salmonella (P = 0.016 for PRO and BUT vs. CON). In addition, PRO treatment had a positive effect on behavioral displays, particularly exploring (P &lt; 0.05 vs. CON), feeding (P &lt; 0.05 vs. CON and BUT) and other active behaviors (P &lt; 0.05 vs. CON and BUT) in the morning period (0830 to 1030 h). In the afternoon (1400 to 1600 h), the challenge effect was most significant. Pigs were less active after the challenge (P &lt; 0.001), with a decrease in positive contacts (P = 0.004), exploration (P &lt; 0.001) and feeding behaviors (P &lt; 0.001) on d 3 PI, in comparison with before the challenge. Accordingly, many lying conducts increased at d 3 PI (P &lt; 0.05). In conclusion, both treatments had positive effects against Salmonella, and behavior analysis appears to be a sensible tool to be considered.

scholarly journals Lifestyle, environmental exposures and male fertility in healthy young men in North Italy

2019 ◽  
Vol 29 (Supplement_4) ◽  
Author(s):  
C Zani ◽  
D Zani ◽  
F Donato ◽  
M Marullo ◽  
G C V Viola ◽  
...  
Keyword(s):  
Physical Activity ◽  
Semen Quality ◽  
Controlled Trial ◽  
Intervention Group ◽  
Young Men ◽  
Lifestyle Changes ◽  
Abdominal Circumference ◽  
Semen Parameters ◽  
Control Group ◽  
Sperm Analysis

Abstract Background Human semen quality is affected by metabolic, lifestyle and environmental factors. We planned a randomized controlled trial to evaluate the effects of lifestyle changes on semen quality in healthy young men living in Italian highly polluted areas. We present here some preliminary baseline data on the association between lifestyle and semen quality. Methods 150 non-smokers, non-alcohol drinkers and non-obese young healthy men, aged 18-22 years, have been assigned to an intervention or control group according to a 1:1 randomization plan. The intervention group follows a 4-month nutritional pathway based on a Mediterranean dietary pattern and receives suggestions on practice of physical activity. All subjects undergo urologic examination, measurement of weight, height and abdominal circumference, an interview on lifestyle variables, and provide blood and semen samples in fasting conditions, at the enrollment and at the end of the intervention and follow-up (after 8 months). Adherence to Mediterranean diet is assessed using the “PREvención con DIeta MEDiterránea” (PREDIMED) tool, and physical activity using the “International Physical Activity Questionnaire” (IPAQ). Automated sperm analysis for all semen parameters is performed on the SQA-V GOLD. Results The data of 101 subjects living in a highly industrialized area in North Italy (mean±SD: age: 20.3±1.2 years, BMI: 22.3±2.2) were analyzed. The sperm cell concentration was higher in subjects with IPAQ score &gt; median than those with lower values (68.3±48.6 vs 63.7±47.0 106/mL). Total percent motility was higher in subjects with abdominal circumference &lt; median (50.7±13.6 vs 41.2±18.8, p = 0.004), in subjects with IPAQ score &gt; median (48.2±16.8 vs 44.2±16.8), and in subjects with PREDIMED score &gt; 7 (47.6±18.8 vs 45.0±15.0). Conclusions These preliminary data suggest that semen quality is associated with abdominal fat and physical activity in healthy young men. Key messages The semen quality is a sensitive marker of the health status and is predictive of disease occurrence. Our study suggests that semen quality is associated with lifestyle aspects in healthy young men.

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