Resveratrol-Based Multivitamin Supplement Increases Sperm Concentration and Motility in Idiopathic Male Infertility: A Pilot Clinical Study

Background. It is known that a multitude of factors may lead to male factor infertility, but still, in the majority of cases, the cause remains largely idiopathic, reflecting poor understanding of the basic process of spermatogenesis and the mechanisms involved. Resveratrol is a polyphenol compound that displays several cellular aspects mainly associated with SIRT1-pathway activation and promotion of mitochondrial enhancer activities. In several animal models, resveratrol has shown positive effects on mitochondria and membrane potential. This could explain effects on sperm concentration and motility. The aim of this study is to evaluate the effects on the semen parameters of GENANTE®, a multivitamin supplement containing 150 mg of resveratrol/day, in patients with idiopathic infertility. Methods. This was a prospective single center clinical study. Twenty patients took a multivitamin supplement based on 150 mg of resveratrol (GENANTE®), in the form of an oral tablet every 12 h, and were followed up at 1, 3, and 6 months after treatment. Pre- and post-treatment evaluation included history, clinical examination, semen analysis, hormonal determinations, and scrotal and prostatic ultrasound. Results. Our preliminary pilot study demonstrated that the multivitamin supplement based on resveratrol improves sperm motility (48.3% ± 13.8 vs. 59.0% ± 12.8, p = 0.0001) and concentration (22.6 × 106/mL ± 9.5 vs. 25.7 × 106/mL ± 8.1, p = 0.0001) after 3 and 6 months of treatment in men with idiopathic infertility. Conclusion. Our data suggest that targeting the metabolic and energetic pathways involved in spermatogenesis and mitochondrial activity could lead to potential effects and counteract subfertility/infertility in men through a mitochondria dynamics mechanism. Trial registration number: ClinicalTrials.gov registration identifier: NCT03864198, registered on 1 January 2019.

Download Full-text

Abnormal semen parameters among males in infertile couples: a cross sectional study from a tertiary care centre

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20203330 ◽

2020 ◽

Vol 9 (8) ◽

pp. 3398

Author(s):

Jyoti Garg ◽

Rachana Meena ◽

Shailaja Shukla ◽

Sunita Sharma ◽

Riva Choudhury

Keyword(s):

Semen Quality ◽

Semen Analysis ◽

Tertiary Care ◽

Sperm Concentration ◽

Semen Parameters ◽

Medical Attention ◽

Male Factor Infertility ◽

Male Factor ◽

Cross Sectional ◽

Infertile Couples

Background: In India, the prevalence of primary infertility ranges from 3.9% to 16.8%. Male factor contributes 40-50% of this. Male factor infertility is indicated by decreased sperm concentration, reduced motility, vitality or abnormal sperm morphology. Semen analysis is the single most important investigation to detect male factor infertility. The aim of this study was to analyse the prevalence of abnormal semen parameters among males in infertile couples and their association with contributing factors.Methods: This cross-sectional hospital-based study was carried out in the Department of Pathology at Lady Hardinge Medical College and Smt. Sucheta Kriplani Hospital. A total of 400 cases were analyzed during a period of 6 months. Detailed history of the couple was taken. Semen analysis was done using automated semen analyzer (SQA-vision) after 3 days of abstinence according to the WHO 5th edition 2010 guidelines. The results were analysed using excel sheet and SPSS software.Results: In the present study, 122 cases (30.5%) out of 400 cases had abnormal semen parameters. Most common abnormality detected was asthenozoospermia (14.3%) followed by oligozoospermia (13.8%), azoospermia (10.5%) and teratozoospermia (10.5%). There was significant association of alcohol intake, obesity and trauma with abnormal semen parameters.Conclusions: Asthenozoospermia was the most common abnormality noted in this study. Lifestyle modifications along with timely medical attention in male partners of infertile couples can improve the semen quality.

Download Full-text

Pattern of semen analysis at andrology lab of Bangabandhu Sheikh Mujib Medical University: Findings and the shortcomings to overcome

Bangabandhu Sheikh Mujib Medical University Journal ◽

10.3329/bsmmuj.v7i2.29444 ◽

2016 ◽

Vol 7 (2) ◽

pp. 103

Author(s):

Shakeela Lshrat ◽

Parveen Fatima ◽

Nurjahan Begum ◽

Afruna Rahman Diti

Keyword(s):

Primary Care ◽

Semen Analysis ◽

Sperm Concentration ◽

Descriptive Study ◽

Semen Parameters ◽

Who Guidelines ◽

Male Factor ◽

Infertile Men ◽

Infertile Couples ◽

Medical University

Background: The Infertility wing dept. of gyne & obst at BSMMU provides services to the infertile couples who are referred from primary care levels and who cannot afford the expensive private facilities. The semen analysis is performed for the male partners of infertile couples at the Andrology Lab of BSMMU for detecting male factor abnormalities. Objectives: The objective of the study is to find out the pattern of semen parameters in our population and to find out the frequency and type of abnormal semen parameters. Methods: This is a retrospective descriptive study of the semen analysis performed at the Andrology Lab of BSMMU during the year 2011. A total of 200 consecutive samples were analysed. The procedure and reference values were according to the WHO guidelines 1999. Results: Semen parameters were abnormal in 38.5% of semen analysis. Severe male factor abnormality (azospermia and severe oligospermia combined) was in 28%. Sperm concentration had the highest variability followed by motility and morphology respectively in the n01moozospermic males. Conclusion: Severe oligospennia and azospermia are the most common abnormalities among the infertile men presenting at the Infertility unit ofBSMMU. It is recommended that the service at the Infertility wing of BSMMU should be more focused on these male factor abnormalities.

Download Full-text

A study of abnormal semen parameters in infertile couples in Assam, India

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20190870 ◽

2019 ◽

Vol 8 (3) ◽

pp. 997

Author(s):

Parija Juneja ◽

Pranay K. Phukan ◽

Debojit Changmai

Keyword(s):

Semen Quality ◽

Semen Analysis ◽

Care Center ◽

Tertiary Care ◽

Sperm Concentration ◽

Semen Parameters ◽

Male Partners ◽

Male Factor ◽

Volume Viscosity ◽

Infertile Couples

Background: The importance of male factor contribution to infertility is well recognised. Semen analysis is an indispensable diagnostic tool in the evaluation of the male partners of infertile couples. Aim and objective of the study was conducted to determine the abnormalities in semen among male partners in infertile couples.Methods: Detailed history, examination and semen analysis was done. Semen samples were analysed for volume, viscosity, sperm concentration, motility, and morphology, according to WHO guidelines.Results: This study, done at a tertiary care center in north eastern India has demonstrated that abnormal semen quality is a major factor contributing to infertility in couples. Total number of participants were 94. Most of the male partners i.e. 65.96%, who came for infertility evaluation were between 30-39 years of age with 56.38% participants having abnormal semen parameters. The most common abnormality found was asthenozoospermia seen in 35.11% of cases followed by oligozoospermia seen in 25.53% of cases. Teratozoospermia and azoospermia were seen in 7.45% and 4.26% of cases respectively.Conclusions: Males contribute towards infertility in couples significantly. Abnormal semen pattern is a common cause. More research is needed to render more valuable and significant results regarding male infertility.

Download Full-text

A STUDY OF ABNORMAL SEMEN PARAMETERS IN INFERTILE COUPLES IN BHAGALPUR, BIHAR

10.36106/2012124 ◽

2021 ◽

pp. 5-6

Author(s):

Anshu Anshu ◽

Usha Kumari ◽

Debarshi Jana

Keyword(s):

Semen Quality ◽

Semen Analysis ◽

Sperm Concentration ◽

Semen Parameters ◽

Who Guidelines ◽

Male Partners ◽

Male Factor ◽

Obstetrics And Gynaecology ◽

Volume Viscosity ◽

Infertile Couples

Background: The importance of male factor contribution to infertility is well recognised. Semen analysis is an indispensable diagnostic tool in the evaluation of the male partners of infertile couples. Aim and objective of the study was conducted to determine the abnormalities in semen among male partners in infertile couples. Detailed history, examination and Methods: semen analysis was done. Semen samples were analysed for volume, viscosity, sperm concentration, motility, and morphology, according to WHO guidelines. This study, done at Obstetrics and Gynaecology Department of Results: JLNMCH, Bhagalpur, Bihar has demonstrated that abnormal semen quality is a major factor contributing to infertility in couples. Total number of participants were 94. Most of the male partners i.e. 65.96%, who came for infertility evaluation were between 30-39 years of age with 56.38% participants having abnormal semen parameters. The most common abnormality found was asthenozoospermia seen in 35.11% of cases followed by oligozoospermia seen in 25.53% of cases. Teratozoospermia and azoospermia were seen in 7.45% and 4.26% of cases respectively. Males contribute towards infertility in Conclusions: couples signicantly. Abnormal semen pattern is a common cause. More research is needed to render more valuable and signicant results regarding male infertility

Download Full-text

Paper-Based Quantification of Male Fertility Potential

Clinical Chemistry ◽

10.1373/clinchem.2015.250282 ◽

2016 ◽

Vol 62 (3) ◽

pp. 458-465 ◽

Cited By ~ 34

Author(s):

Reza Nosrati ◽

Max M Gong ◽

Maria C San Gabriel ◽

Claudio E Pedraza ◽

Armand Zini ◽

...

Keyword(s):

Sperm Motility ◽

Male Fertility ◽

Semen Analysis ◽

Sperm Concentration ◽

Absolute Humidity ◽

Semen Parameters ◽

Motile Sperm ◽

Clinical Practices ◽

Male Factor ◽

Fertility Potential

Abstract BACKGROUND More than 70 million couples worldwide are affected by infertility, with male-factor infertility accounting for about half of the cases. Semen analysis is critical for determining male fertility potential, but conventional testing is costly and complex. Here, we demonstrate a paper-based microfluidic approach to quantify male fertility potential, simultaneously measuring 3 critical semen parameters in 10 min: live and motile sperm concentrations and sperm motility. METHODS The device measures the colorimetric change of yellow tetrazolium dye to purple formazan by the diaphorase flavoprotein enzyme present in metabolically active human sperm to quantify live and motile sperm concentration. Sperm motility was determined as the ratio of motile to live sperm. We assessed the performance of the device by use of clinical semen samples, in parallel with standard clinical approaches. RESULTS Detection limits of 8.46 and 15.18 million/mL were achieved for live and motile sperm concentrations, respectively. The live and motile sperm concentrations and motility values from our device correlated with those of the standard clinical approaches (R2 ≥ 0.84). In all cases, our device provided 100% agreement in terms of clinical outcome. The device was also robust and could tolerate conditions of high absolute humidity (22.8 g/m3) up to 16 weeks when packaged with desiccant. CONCLUSIONS Our device outperforms existing commercial paper-based assays by quantitatively measuring live and motile sperm concentrations and motility, in only 10 min. This approach is applicable to current clinical practices as well as self-diagnostic applications.

Download Full-text

Comparison of 3- and 6-Month Outcomes of Combined Oral L-Carnitine Fumarate and Acetyl-L-Carnitine Therapy, Included in an Antioxidant Formulation, in Patients with Idiopathic Infertility

American Journal of Men s Health ◽

10.1177/15579883211036790 ◽

2021 ◽

Vol 15 (5) ◽

pp. 155798832110367

Author(s):

Marek Szymański ◽

Tomasz Wandtke ◽

Karolina Wasilow ◽

Marek Andryszczyk ◽

Radosław Janicki ◽

...

Keyword(s):

Sperm Count ◽

Coenzyme Q ◽

Sperm Concentration ◽

World Health Organisation ◽

Human Reproduction ◽

World Health ◽

Semen Parameters ◽

Male Factor ◽

Idiopathic Infertility ◽

Male Gametes

The male factor is responsible for infertility in about 35–40% of all cases. Idiopathic oligo- and/or astheno- and/or therato-zoospermia is one of the most common male fertility disorders and remains a significant therapeutic challenge. The primary cause of idiopathic male infertility remains unknown but seems to be associated with oxidative stress. Objective: The use of antioxidative formulation to improve qualitative and quantitative deficiencies in the male gametes. In total, 78 subjects were treated with a combination of 1,725 mg L-carnitine fumarate, 500 mg acetyl-L-carnitine, 90 mg vitamin C, 20 mg coenzyme Q10, 10 mg zinc, 200 µg folic acid, 50 µg selenium, and 1.5 µg vitamin B12 (Proxeed® Plus, Sigma-Tau, Italy) for 6 months; the preparation was taken twice daily from the time idiopathic infertility was diagnosed. Basic seminal parameters were evaluated by a European Society of Human Reproduction and Embryology (ESHRE) -certified embryologist following the fifth edition of the World Health Organisation (2010) guidelines at three time points: at baseline and 3 and 6 months of treatment. Improvements in semen parameters (differing in terms of dynamics) were evident at 3 months and gradually improved over the 6 months of treatment. Each parameter: sperm concentration, total sperm count, sperm total and progressive motility improved significantly after treatment except for the percentage of sperm of abnormal morphology and ejaculate volume. Proxeed Plus was effective for patients with idiopathic infertility; however, a long treatment period is needed to achieve optimal results.

Download Full-text

Impact of percutaneous embolization versus subinguinal microsurgical ligation on semen parameters in primary varicocele patients: comparative study

Egyptian Journal of Radiology and Nuclear Medicine ◽

10.1186/s43055-020-00370-4 ◽

2020 ◽

Vol 51 (1) ◽

Author(s):

Haytham M. Nasser ◽

Ahmed Hussein ◽

Gad M. Behairy ◽

Mostafa Abdo

Keyword(s):

Sperm Count ◽

Semen Analysis ◽

Statistical Significance ◽

Semen Parameters ◽

Male Factor ◽

Percutaneous Embolization ◽

Significant Difference ◽

Group 2 ◽

Group 1

Abstract Background Varicocele is an abnormally dilated pampiniform plexus of the veins within the spermatic cord and is considered the most common correctable cause of male factor infertility. Many approaches are described for treatment either surgical (tradition inguinal, subinguinal, and laparoscopic) or non-surgical percutaneous embolization. During the period from August 2017 to December 2018, we prospectively analyzed the preoperative and post-operative alteration of semen parameters (at 3 and 9 months) of the data collected from 63 patients with clinically evident varicocele referred to our tertiary hospital. Patients were divided into two groups: group 1, thirty-three patients who underwent subinguinal microsurgical ligation, and group 2, thirty patients who underwent percutaneous embolization. Results Sixty-three patients enrolled in this study were divided in two groups: group 1, patients who underwent surgery, and group 2, patients who underwent embolization; the mean age is 24.6 ± 1.27 years in group 1 and 23.7 ± 2 years in group 2; there was no statistically significant difference between the two groups as regards BMI, diabetes, hypertension, and smoking. Bilaterality was present in 15.2% of group 1 patients and 10% in group 2 patients (P value 0.06). Most of the patients were classified as grades 2 and 3 with no statistical significance regarding severity of the disease. Preoperative semen parameters for patients including sperm count, motility, and abnormal forms showed no statistically significant difference between the two groups. Post-intervention semen analysis was done twice during follow-up after 3 months and 9 months from the date of intervention. After 3 months, the semen parameters were improved in both groups in spite of the higher sperm count in group 2 but with no statistical significance. After 9 months follow-up, semen analysis showed persistent increase in sperm mobility in group 1 patients in comparison to group 2 patients. Both groups had better improvement in count of normal form with no statistical significant change. Conclusion Improvement of semen parameters while treating primary varicocele by either subinguinal microsurgery approach or percutaneous embolization shows equivalent outcomes.

Download Full-text

Randomized Clinical Trial: Effect of Walnuts on Semen Parameters and Male Fertility (P18-042-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz039.p18-042-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Author(s):

Wendie Robbins ◽

Howard Kim ◽

Justin Houman ◽

Geng-Wei Lee

Keyword(s):

Clinical Trial ◽

Los Angeles ◽

Randomized Clinical Trial ◽

Sperm Motility ◽

Clinical Care ◽

Sperm Concentration ◽

Nutritional Supplement ◽

Semen Parameters ◽

Male Factor Infertility ◽

Male Factor

Abstract Objectives Infertility affects ∼8–12% of couples worldwide with ∼40% attributed to male factors. Recent studies suggest a role for paternal diet in fertility. Walnuts contain a variety of nutrients essential in the development of spermatozoa. We conducted a randomized clinical trial (RCT) to determine if consumption of walnuts improves semen parameters and fertility in men seeking clinical care for male factor infertility. Methods This was a two arm, single blind, RCT. The comparison groups both received usual care for male factor infertility. One group added 42 gm/d walnuts to their diet, and the other group added a daily nutritional supplement recommended for male reproductive health. Participants (n = 75) were enrolled at an infertility clinic located in a large metropolitan medical center. Eligibility was determined by history, physical exam, and lab tests collected as part of clinical care. Research measures included semen analysis and blood sample at baseline and 3 months; ASA24 dietary recall at baseline, 2 and 3 months; and fertility report at 3 months and 1 year. Results Age range was 27 to 61 years (39.7 ± 7.0); BMI range 19.6 to 46.9 (26.8 ± 4.5); participant race was Asian 26.2%, White 44.3%, Hispanic White 16.4%, Black 8.2%, other 4.9%. Baseline sperm concentration was 39.4 ± 30 million per ml; sperm motility 31.1 ± 23.4%; and progressive motility 21.2 ± 15.8%. At 3 months, the walnut group demonstrated increased sperm motility and concentration, P = .04 and P = .07, respectively, whereas no significant changes from baseline were found in the nutritional supplement group. Both groups showed improved sperm morphology, P < .03. Preliminary data from the subset of men with 1-year follow-up data shows higher frequency of pregnancy in the walnut group compared to nutritional supplement, although not statistically significant, P = .09. We continue to follow the remaining participants until their 1-year fertility report. Conclusions This RCT demonstrated a beneficial effect of adding walnuts to the diet on sperm motility and morphology in men seeking care for infertility. Preliminary fertility data suggests walnuts may enhance the probability of pregnancy for men with male factor infertility. Funding Sources Center for Occupational and Environmental Health, University of California, Los Angeles; California Walnut Commission.

Download Full-text

ASSOCIATION OF VARICOCELE WITH OLIGOSPERMIA AND AZOSPERMIA

Journal of Saidu Medical College, Swat ◽

10.52206/jsmc.2013.3.2.303-307 ◽

1969 ◽

Vol 3 (2) ◽

pp. 303-307

Author(s):

NAIK ZADA ◽

SHAFI ULLAH KHAN ◽

RIAZ AHMAD KHAN

Keyword(s):

Sperm Count ◽

Human Subjects ◽

Semen Analysis ◽

Cross Sectional Study ◽

Semen Parameters ◽

Male Factor ◽

Cross Sectional ◽

Seminal Parameters ◽

The Mean ◽

Age Range

OBJECTIVE: To determine the frequency of abnormal semen parameters among patients presenting withvaricocele1METHODS:It was a descriptive cross-sectional study conducted at the Department of Urology Institute ofkidney diseases Hayat Abad Medical Complex Peshawar and Cenna hospital Saidu Sharif Swat. The studywas carried out on 139 human subjects with clinical evidence of varicocele between age range of 15-45years.The diagnosis of varicocele was based on palpable and/or visible scrotal lump of testicular veins(pampiniform plexus) and was diagnosed on the basis of clinical examination. Semen analysis was carriedout in all these patients and information wascollected on pre designed proforma.RESULTS:The study included a total of 139 patients with varicocele. The mean age of patient was 30 years(15-45) among the patients having symptoms of varicocele. The Mean ±SD for duration of varicocelesymptoms was 9.32 ± 9.70 months. 6.5% (n=9) patients were having azoospermia and 20.1% (n=28)patients had oligozoospermia.CONCLUSION: Patients with varicocele have poor seminal parameters in terms of sperm count i.e.oligozoospermia and azoospermia responsible for male factor infertility in majority ofcases.KEYWORDS:varicocele;seminal parameters;sperm count, infertility

Download Full-text

The Effects of Combined Oral Antioxidants on Male Infertility

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v9ispl1.1290 ◽

2018 ◽

Vol 9 (SPL1) ◽

Author(s):

Ali Hadi Sabhan

Keyword(s):

Male Infertility ◽

Semen Analysis ◽

Single Agent ◽

Sperm Concentration ◽

Membrane Integrity ◽

Semen Parameters ◽

Duration Of Treatment ◽

Male Patients ◽

Initiation Of Therapy

High reactive oxygen species (ROS) levels was considered as an important factor of male infertility that can result in DNA damage, decrease motility and damage membrane integrity. The role of antioxidants is to maintain the balance between ROS production and their clearance which improve sperm parameters and enhance male fertility. Sixty six male patients with median age of 29 years with a mean period of infertility of 1.5 year that attend the infertility clinic in Al-Diwaniya Maternity and Pediatric Hospital were selected randomly in this study which extend from January 2013 to October 2014 (22 months).The patients were selected to have idiopathic oligo-astheno-teratozoospermia,they underwent a detailed history,general and genital physical examination,Two semen analyses,baseline hormone profile and scrotal Doppler ultrasound examination. All patients were given a combination of oral antioxidants including vitamin E (400 IU. once daily),coenzyme Q10 (75 mg. tablet twice a day),zinc sulphate (15 mg. tablet three time a day) and L-carnitine (1000 mg. twice a day) for three months duration of treatment. Semen analysis was repeated after three months from initiation of therapy and was evaluated for changes in volume,concentration,motility and morphology in comparison with baseline semen analyses and any pregnancy was also documented. Thirteen patients (19%) get pregnancy after a period of 2 to 3 months,other patients show variable changes in their seminal analysis after 3 months. Thirty patients (45%) show increasing in seminal volume of 0.5 to 2 ml., while the sperm concentration was increased in 28 patients (42%). Twenty two patients (33%) show increasing in motility, morphology was increased in 34 patients (51%).From all 66 patients; 6 patients (9 %) get improvement in all four parameters,while 9 patients (14 %) get improvement in only 3 parameters,20 patients (30 %) get improvement in only 2 parameters,23 patients (35 %) get improvement in only one parameter and 8 patients (12 %) had no improvement in any parameter. This study demonstrates a possible role of combined oral antioxidants in the improvement of semen parameters and pregnancy rate for selected infertile men with superiority for the use of combination of antioxidants rather than single agent treatment.

Download Full-text