scholarly journals Effects of Umbilical Cord Blood Stem Cells on Coronary Artery Damage in Mice with Kawasaki Disease By High-Frequency Ultrasound

2021 ◽  
Vol 1 (1) ◽  
pp. 1-5
Author(s):  
Na Zhao ◽  
◽  
Xue-mei Zhang ◽  
Hua Guo ◽  
◽  
...  
Keyword(s):  
Stem Cells ◽  
Coronary Artery ◽  
High Frequency ◽  
Control Group ◽  
Model Group ◽  
High Frequency Ultrasound ◽  
Coronary Artery Diameter ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells ◽  
Frequency Ultrasound

Objective: to use lactobacillus casein cell wall extract (LCWE) to induce Kawasaki disease (KD) mouse model, and analyse the process of coronary artery damage and effects of umbilical cord blood stem cells by high-frequency ultrasound in small animals. Methods: LCWE was prepared and 18 BALB/c pups were randomly divided into two groups: 15 in KD model group and 3 in the normal control group. KD model was induced by a single intraperitoneal injection of 0.5ml LCWE in the model group, and changes of the coronary artery were observed at 2d, 21d and 30d after the injection, respectively. From the 16th day of modeling, 300 μL PBS was injected intraperitoneally daily in the control group and model group. The hUC-MSCs 300 μL (105/mL) were intraperitoneally injected daily for 10 consecutive days in the stem cell group. The mice were sacrificed in batches on day 2, 15, 21 and 30, and the morphological changes of coronary arteries were observed by echocardiography and histopathology. Results: the change of coronary artery diameter could be accurately measured by high-frequency small animal ultrasound. At 21d, the coronary arteries of the model group were widened compared with those of the control group. At 30d, there was no significant difference between the model group and the previous model group. Histopathology showed slight swelling of the epicardium of aortic valve, mitral valve, right ventricle and atrium, scattered infiltration of a few neutrophils, dilatation of the coronary artery lumen, necrosis and disintegration of a small number of myocardial cells, and significant hyperplasia of local fibrous connective tissue accompanied by solid calcium salt deposits. After hUC-MSCs intervention treatment, B-ultrasound showed a decrease in the main coronary artery diameter, histopathology showed multiple lymphocytes, eosinophilic granulocytes, and mononuclear cell infiltration in the left atrial appendage of mice, and no obvious vascular inflammatory reaction or other obvious abnormalities were observed. Conclusion: high-frequency ultrasound can be used to clearly obtain the coronary artery image of KD mice and dynamically observe the evolution process of coronary artery diameter, which provides more diagnostic basis for the treatment of clinical KD. And hUC-MSCs intervention reduced the pathological lesion of coronary artery inflammation in mice compared with the model group.

Efficiency Control Cellulite Treatment Using High-Frequency Ultrasound Visualization of High Resolution

10.12737/5809 ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 1-2
Author(s):  
Белков ◽  
P. Belkov ◽  
Безуглый ◽  
A. Bezuglyy ◽  
Круглова ◽  
...  
Keyword(s):  
High Resolution ◽  
Human Skin ◽  
High Frequency ◽  
Subcutaneous Tissue ◽  
Control Group ◽  
Objective Data ◽  
High Frequency Ultrasound ◽  
Functional Parameters ◽  
Efficiency Control ◽  
Frequency Ultrasound

To describe ultrasonographic image of human skin with cellulite, the authors used high-frequency ultrasound visualization of the skin with a frequency of 22 MHz using a system DUB (tpm GmbH Germany). The skin on the thigh in 15 patients with cellulite and in 10 patients in the control group was examined. The differences in thickness and acoustic density of dermis and subcutaneous tissue between the group of patients with cellulite and in control were described. Objective data of high frequency ultrasound allow to quantify morphological and functional parameters of the skin in the dynamics and results of cellulite correction.

scholarly journals Changes in the microcirculation of the oral mucosa in post-COVID-19 patients not receiving anti-coagulant therapy

2022 ◽  
Vol 20 (4) ◽  
pp. 95-100
Author(s):  
M. V. Chubarnova ◽  
A. B. Davydov ◽  
V. A. Esin ◽  
O. B. Davydova ◽  
I. O. Kostin
Keyword(s):  
Blood Flow ◽  
Oral Mucosa ◽  
High Frequency ◽  
Control Group ◽  
High Frequency Ultrasound ◽  
Flow Rates ◽  
Mean Values ◽  
Coronavirus Infection ◽  
The Mean ◽  
Frequency Ultrasound

Introduction. The outbreak of a new coronavirus infection has become a challenge for the global health system. The COVID-19 infection is directly related to various disorders of the cardiovascular system, including the microcirculatory bed, caused by thrombotic events and deteriorations of blood rheology. Aims. The paper reports on the results of a study of Doppler sonographic parameters changes in patients with a novel coronavirus infection over the past 6 months. Materials and methods. We assessed the oral mucosa microcirculation in three segments using the high-frequency ultrasound dopple-rography. Results. We recorded the linear and volumetric blood flow rates and the Gosling and Purselo indexes in the course of our work. When comparing the obtained average statistical parameters of blood flow velocity, the linear and volumetric blood flow rates in patients of both groups were found to be lain in the same range and the mean values of Vas, Vam, Qas were equal. The mean values of the Purselo resistance index were closer to 1,0 in patients with COVID-19, and the values of the Gosling pulsation index (PI) were on average 53.3 % higher than in the control group. Conclusion. We evaluated the screening capabilities and potential of high-frequency ultrasound dopplerography for use in patients of different age groups and different somatic status.

scholarly journals Study of the influence of different methods of application of Metrogyl Denta gel on the microbiota of periodontal pockets in periodontitis

2021 ◽  
Vol 25 (1) ◽  
pp. 42-47
Author(s):  
Dmitry I. Morozov ◽  
Alexander G. Volkov ◽  
Egor E. Olesov ◽  
Nataliya Zh. Dikopova ◽  
Yulia A. Trefilova
Keyword(s):  
High Frequency ◽  
Periodontal Diseases ◽  
Practical Interest ◽  
Control Group ◽  
Periodontal Tissue ◽  
High Frequency Ultrasound ◽  
Periodontal Tissues ◽  
Group Data ◽  
And Control ◽  
Frequency Ultrasound

BACKGROUND: One of the most popular antibacterial drugs used in periodontology is Metrogil Denta gel. In recent years, it is most often prescribed as a means of local antibacterial therapy. However, there is practically no information about the use of Metrogil Denta gel ultraphonophoresis in the treatment of periodontal tissue diseases. Therefore, the development of the Metrogil Denta gel ultraphonophoresis technique and the study of its effectiveness in inflammatory periodontal diseases has significant theoretical and practical interest. AIM: This study aimed to explore the influence of various methods of using Metrogyl Denta gel on the microbiota of the periodontal pockets in periodontitis. MATERIALS AND METHODS: In the first group (main), composed of 20 patients with a diagnosis of moderate periodontitis, the treatment procedures included high-frequency ultrasound and application of antibacterial preparation of Metrogyl Denta gel for the periodontal tissue (ultraphonophoresis of Metrogyl Denta gel). In the second group (control), composed of 20 patients with a diagnosis of moderate periodontitis, phonophoresis was not prescribed, and Metrogyl Denta gel was applied into the surface of the gums of the upper and lower jaw two times a day for 10 days. RESULTS: Contents of the pathological periodontal pockets were examined twice: first after dental plaque removal in patients with periodontitis of the main and control groups and then after 10 ultraphonophoresis procedures using Metrogyl Denta gel as a contact medium in the study group or after 10 days of Metrogyl Denta gel applications in the control group. Data analysis revealed that ultraphonophoresis of Metrogyl Denta gel has a more pronounced antibacterial effect in comparison with the injection of this drug. CONCLUSIONS: The combined effect of high-frequency ultrasound and Metrogyl Denta gel makes it possible to create a drug reserve in the periodontal tissues.

Abstract 443: Absence of Pro-Protein Convertase Subtilisin/Kexin 6 Increases Flow-Mediated Outward Remodeling in the Mouse Common Carotid Artery

2017 ◽  
Vol 37 (suppl_1) ◽  
Author(s):  
Samuel Röhl ◽  
Anders Arner ◽  
Kenneth Caidahl ◽  
Ulf Hedin ◽  
Ljubica Perisic Matic ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Common Carotid Artery ◽  
High Frequency ◽  
Matrix Metalloproteases ◽  
Histological Evaluation ◽  
Control Group ◽  
High Frequency Ultrasound ◽  
Post Surgery ◽  
The Right ◽  
Frequency Ultrasound

Objective: Proprotein convertases (PCSKs) process matrix metalloproteases and cytokines but their function in vasculature is largely unknown. Previously, we demonstrated upregulation of PCSK6 in atherosclerotic plaques, localization to smooth muscle cells in the fibrous cap and positive correlation to inflammation, extracellular matrix remodeling and cytokines. Here, our aim was to evaluate the effects of PCSK6 on flow-mediated vascular remodeling in mice using high-frequency ultrasound and myography. Materials and Methods: PCSK6 -/- and C57Bl/6J mice were compared in this study divided in baseline control and increased flow groups. Increased flow was created in the right common carotid artery (CCA) by ligation of the left CCA. All animals were subjected to high-frequency ultrasound examination prior to surgery, at 3 and 5 weeks after surgery. Upon euthanization 6 weeks post-surgery the right CCA was harvested for myography evaluation, subsequently fixed at optimal stretch and prepared for histological evaluation. Results: The vascular circumference at optimal stretch in myography was strongly correlated (Pearson r=0.74, p<0.001) and in agreement with the diastolic circumference measured by high-frequency ultrasound in examined animals. A significant increase in diastolic circumference was seen at 3 and 5 weeks after surgery in PCSK6 -/- mice with increased flow compared to PCSK6 -/- control group (1.6±0.15 mm vs 1.4±0.08 mm, p<0.05 and 1.7±0.09 mm vs 1.4±0.12 mm, p<0.01). Myography revealed a significant increase in circumference at optimal stretch (1.7±0.21 mm vs 1.4±0.08 mm, p<0.05) in PCSK6 -/- mice subjected to increased flow compared to PCSK6 -/- control group. A significant flow-mediated increase in medial contractility was identified (0.68±0.14 mN/mm vs 0.45±0.11 mN/mm, p<0.05) in C57Bl/6J mice compared to C57Bl/6J control where as an absence of flow-mediated increase in medial contractility was seen in PCSK6 -/- mice. Conclusion: Absence of PCSK6 increases outward remodeling and reduces medial contractility in response to increased blood flow.

scholarly journals Coronary artery imaging with intravascular high-frequency ultrasound.

Circulation ◽  
1990 ◽  
Vol 81 (5) ◽  
pp. 1575-1585 ◽  
Author(s):  
B N Potkin ◽  
A L Bartorelli ◽  
J M Gessert ◽  
R F Neville ◽  
Y Almagor ◽  
...  
Keyword(s):  
Coronary Artery ◽  
High Frequency ◽  
Coronary Artery Imaging ◽  
High Frequency Ultrasound ◽  
Frequency Ultrasound

Arterial Thrombus Dissolution in Vivo Using a Transducer-Tipped, High-Frequency Ultrasound Catheter and Local Low-Dose Urokinase Delivery

2001 ◽  
Vol 8 (3) ◽  
pp. 282-290 ◽  
Author(s):  
Shaul Atar ◽  
Huai Luo ◽  
Tomoo Nagai ◽  
Roger A. Sahm ◽  
Michael C. Fishbein ◽  
...  
Keyword(s):  
Drug Delivery ◽  
High Frequency ◽  
Low Dose ◽  
Group Versus ◽  
Local Drug Delivery ◽  
Control Group ◽  
High Frequency Ultrasound ◽  
Delivery Catheter ◽  
Frequency Ultrasound

Purpose: To examine the hypothesis that a transducer-tipped high-frequency ultrasound drug-delivery catheter may augment the thrombolytic effects of locally delivered low-dose urokinase and result in improved recanalization rates and reduced residual thrombotic burden. Methods: Thrombi were induced in situ bilaterally in 5- to 6-cm-long segments of the superficial femoral arteries in 9 dogs by intraluminal thermal damage and injection of thrombin. A transducer-tipped high-frequency local drug-delivery catheter was applied at 1.1 MHz and 0.6 W for 60 minutes to one superficial femoral artery segment, and an identical catheter with an inactivated ultrasound transducer was used to treat the contralateral control segment. Urokinase (5000 IU/kg) was delivered bilaterally into the thrombi during the treatment interval. Results: Angiography documented TIMI grade 2 or 3 flow in 9 (100%) segments in the ultrasound-treated group versus 6 (67%) of the controls (no ultrasound) (p = 0.058). Angiographically detected distal embolization was found in 2 ultrasound-treated segments compared with 5 controls (p = 0.02). Protruding or occlusive thrombi were seen angioscopically in 8 (89%) control segments but in only 1 (11%) of the ultrasound-treated arteries (p < 0.001). By histopathology, 7 (78%) segments in the control group had occlusive thrombi, whereas only 3 nonocclusive thrombi were found in the ultrasound-treatment group (p < 0.001). Conclusions: Catheter-delivered high-frequency ultrasound and local low-dose urokinase infusion is efficacious for the treatment of acute thrombotic occlusions as evaluated by angiography, angioscopy, and histopathology.

Sign in / Sign up

Export Citation Format

Share Document