scholarly journals The Effect of Nanozeolite Concentration in a Delivery System of HaNPV1 to the Lethal Time against Crocidolomia pavonana

2020 ◽  
Vol 43 (4) ◽  
Author(s):  
Ikhsan Gatot Aji Prasetio ◽  
Wawan Hermawan ◽  
Mia Miranti ◽  
Camellia Panatarani ◽  
I Made Joni ◽  
...  
Keyword(s):  
Delivery System ◽  
Nuclear Polyhedrosis Virus ◽  
Block Design ◽  
Nanosized Particles ◽  
Entire Surface ◽  
Randomized Block Design ◽  
Milling Method ◽  
Lethal Time ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

The constraints on the effectiveness of nuclear polyhedrosis virus (NPV) as biocontrol are usually due to environmental factors such as temperature and ultraviolet (UV) exposure. Zeolite has been commonly used as a carrier or delivery system for nuclear polyhedrosis viruses. In this study, zeolite powder was reduced into nanosized particles by beads milling method and was investigated for the effect of its concentration in the delivery system of Helicoverpa armigera nuclear polyhedrosis virus (HaNPV1) on the lethal time against the larvae Crocidolomia pavonana. The formulation used three concentrations of nanozeolite suspension, 0.5, 1, 1.5, and 2 wt.% applied for each 4 × 107 of HaNPV1. A randomized block design (RBD) method was applied with 3 replications. The results showed that the scanning electron microscope (SEM) from nanozeolite was seen coating the entire surface of the HaNPV1 polyhedra and an increase of zeolite concentration caused acceleration of the lethal time of C. pavonana instar III. Thus, the fastest lethal time was 1.2 days receiving a concentration of 2 wt.%, which was significantly higher compared to without delivery (2.9 days). The increase of the zeolite concentration up to 2 wt.% in the delivery system for HaNPV1 improved their performance on lethal time and mortality against C. pavonana. It was concluded that nanozeolite as a delivery system enhanced and created a synergy in infecting C. pavonana.

scholarly journals Pengujian Bahan Formulasi MsNPV ( Mythimna separata Nuclear Polyhedrosis Virus) terhadap Ulat Grayak Padi, Mythimna separata Walker di Lapangan

2016 ◽  
Vol 6 (1) ◽  
pp. 15
Author(s):  
Trisnaningsih Trisnaningsih ◽  
Arifin Kartohardjono
Keyword(s):  
Sea Level ◽  
Nuclear Polyhedrosis Virus ◽  
Block Design ◽  
Mythimna Separata ◽  
Life And Death ◽  
Randomized Block Design ◽  
Nuclear Polyhedrosis ◽  
And Control ◽  
Rice Plantation ◽  
Army Worm

The objective of these studies were to observe efficiency material formulations of MsNPV and the influence to rice plantation on different locations (Indramayu (3 m above sea level), Sukabumi (400m above sea level) and Bogor (250 m above sea level) to larvae of rice army worm. This study used randomized block design with 4 treatments consisted of 3 formulation materials (talc, kaolin and gypsum) and control in five replications. Observations were done to life and death larvae on 5, 10, 15 days after inoculation and leaf damaged. Data were analysed with DMRT. Results from this study indicated that material talc formulation was more effective because all the material was dissolve as compare to material kaolin and gypsum formulations while the material were precipitated. Utilize these three material formulations on three different locations above sea level gave the same impact to the mortality army worm larvae and also to the plant damage.

scholarly journals Biological Test of Formulation of Subculture Helicovepa armigera Nuclear Polihedrosis Virus (HaNPV) on Mortality of Spodoptera litura Larvae infested to cabbage (Brassica oleracea Var. capitata Linn.) Plantation

2015 ◽  
Vol 2 (1) ◽  
pp. 646
Author(s):  
Desy Efriyani Anggraeny Nasution ◽  
Mia Miranti ◽  
Melanie .
Keyword(s):  
Brassica Oleracea ◽  
Helicoverpa Armigera ◽  
Spodoptera Litura ◽  
Nuclear Polyhedrosis Virus ◽  
Insect Pests ◽  
Block Design ◽  
Economic Value ◽  
Helicoverpa Armígera ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

<p>Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV) is one of the biological agents that could be used for the development of organic farming in Indonesia. One of the organic agricultural food products which have high economic value is cabbage, HaNPV effectif to control the population of several species of insect pests, can be used instead of synthetic insecticides in agricultural development efforts of organic cabbage. HaNPV formulation can be used as a way to maintain the virulence properties of the virus and facilitate the storage and application. The preparation of subculture HaNPV formulations was in liquid, powders, cornstarch and talk carrier material. The design experimental was used randomized block design consists single factor (type HaNPV formulation consisting of powder formulations, liquid formulation, formulation in cornstarch, and talc formulation including water formulation without virus as a control), with eight replications. The results showed that all of the formulations of subculture HaNPV causing larvae mortality at range 97.4% -100%. The highest mortality of larvae occurred in cabbage plants are protected by HaNPV carrier liquid and talk preparations by 100% each. <br /><strong></strong></p><p><strong>Keywords</strong>: Formulations Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV), subculture, Spodoptera litura, Brassica oleracea Var. capitata Linn.</p>

scholarly journals EFEK PENYIMPANAN TERHADAP VIRULENSI NUCLEAR POLYHEDROSIS VIRUS YANG DIAPLIKASIKAN PADA TANAMAN KEDELAI TERSERANG ULAT GRAYAK (Spodoptera litura Fabr. )

2001 ◽  
Vol 1 (1) ◽  
pp. 29-32
Author(s):  
Sudi Pramono
Keyword(s):  
Spodoptera Litura ◽  
Field Experiments ◽  
Nuclear Polyhedrosis Virus ◽  
Block Design ◽  
Control Techniques ◽  
Polyhedrosis Virus ◽  
One Year ◽  
Nuclear Polyhedrosis ◽  
And Control ◽  
Army Worm

One of the problem increased soybean production was Spodoptera litura (army worm), because army worm has been known to be resistant to many insec¬ticides.  Alternatif control techniques should be explored  to reduce population of the pest.  Nuclear Polyhedrosis Virus (NPV) could storage one or more year,  but must used technique cold storage so that not virulence decreased.  This experiment was conducted to study (1) NPV patho¬genicity to army worm in the field, and (2) the effect of storage on the pathogenicity of NPV to S.  litura.  The research consisted of two experiments i.e.  laboratory and field experiments.  Each experiment had  seven treatments arranged in a randomized completely block design.  Each treatment was replicated four times.  The results show  the highest mortality of S.  litura occurred in plot treated  with 1,0 x 107 PIBs /ml, the mortality was 48 to 49,33 spec/plot after ten days aplicated .  If comparative patogenicity the new and the old NPV was not significant.  Population of army worm decreased significantly by NPV and control.  So that patogenecity NPV storage  one  year as well as new NPV  against army worm.     

scholarly journals THE BIOLOGICALTEST OF FORMULATION OF SUBCULTURE Helicoverpa armigera Nuclear PolyhedrosisVirus(HaNPV) against Crocidolomia pavonana Fab. LARVAE POPULATIONTHAT EXPOSED TO CABBAGE (Brassica oleracea var. capitata L.)

2015 ◽  
Vol 2 (1) ◽  
pp. 649
Author(s):  
Anasthasia Vehuliza Surbakti ◽  
Mia Miranti ◽  
Melanie .
Keyword(s):  
Brassica Oleracea ◽  
Helicoverpa Armigera ◽  
Nuclear Polyhedrosis Virus ◽  
Block Design ◽  
Pilot Project ◽  
Water Control ◽  
Randomized Block Design ◽  
Carrier Materials ◽  
Helicoverpa Armígera ◽  
Nuclear Polyhedrosis

<p>The biologicaltest of formulation of subculture Helicoverpa armigera Nuclear Polyhedrosis Virus(HaNPV) against Crocidolomia pavonana Fab.larvae population that exposed to cabbage (Brassica oleracea var. capitata L.) has been done. The subculture HaNPV was formulated in liquid preparations, powders, cornstarch and talc carrier materials, sprayed to C. pavonana larvae population that exposed at cabbage as a pilot project. The method of research used an experimental method, with randomized block design consists of single factor HaNPV and five level formulation there were (p) (liquid dosage forms (p1), powder (p2), and mixed with a carrier such as cornstarch (p3) and talc powder (p4) as well as the provision of water control without virus (p0)) with 8 replications. The density of virus 4x107 polyhedral/ml. The results was statistically analyzed with ANOVA (p&lt;0,05), showed that all formulation HaNPV subculture have equal ability to caused high mortality of C. pavonana larvae population. <br /><strong></strong></p><p><strong>Keywords</strong>: Cabbage, Crocidolomia pavonana, Formulation,HaNPV, Mortality</p>

scholarly journals Enhanced activity of Anticarsia gemmatalis Hüb. (Lepidoptera: Noctuidae) nuclear polyhedrosis virus by boric acid in the laboratory

1997 ◽  
Vol 26 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Lauro Morales ◽  
Flávio Moscardi ◽  
Daniel R. Sosa-Gómez ◽  
Fábio E. Paro ◽  
Ivanilda L. Soldorio
Keyword(s):  
Boric Acid ◽  
Nuclear Polyhedrosis Virus ◽  
Larval Mortality ◽  
Lethal Concentration ◽  
Anticarsia Gemmatalis ◽  
Median Lethal Concentration ◽  
Lethal Time ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Lepidoptera Noctuidae

Boric acid concentrations (0.02,0.03,0.045,0.067 and 0.101 g/100 ml of diet) were evaluated in combination with the Anticarsia gemmatalis Hüb. nuclear polyhedrosis virus (AgNPV) for enhanced virali activity against the insect. Seven days after inoculation, the median lethal concentration (LC50) was 1.52 x 10(5) for the AgNPV alone and 7.95 x 10² for the NPV mixed with 0.045g of boric acid/100 ml of diet. At subsequent evaluation dates (9,11 and 14 days after inoculation) LC50's for NPV+boric acid were ca. 4x lower than those observed for the NPV alone. The median lethal time (LT50) was 13.6 days when the NPV was used alone, while, when in mixture with increasing concentrations of boric acid, LT50 values ranged from 13.7 days (boric acid at 0.02g/100 ml of diet) to 7.4 days (boric acid at 0.101 g/ml of diet). Therefore, boric acid added to the AgNPV significantly increased A. gemmatalis larval mortality and shortened mortality time by the pathogen.

Field Efficiency of Certain Biopesticides and Neem Products Against Helicoverpa armigera (Hubner) on Chickpea (Cicer arietinumL.)

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Nihad H. Mutlag ◽  
Ameer S. A. Al-Haddad
Keyword(s):  
Helicoverpa Armigera ◽  
Block Design ◽  
Individual Treatment ◽  
Neem Oil ◽  
Larval Population ◽  
Neem Cake ◽  
Combination Treatments ◽  
Randomized Block Design ◽  
Helicoverpa Armígera ◽  
Nuclear Polyhedrosis

A field study was conducted to evaluate the efficiency of four microbial insecticides viz. Beauveria bassiana; HaNPV (Helicoverpa armigera Nuclear Polyhedrosis virus); (Bacillus thuringiensisvar.kurstaki 2 gm/L); HaNPV+Bt; neem oil; neem cake and D.D.V.P EC 76% @0.05% at Research Farm SHIATS,Allahabad during rabi season of 2011-2012. The experiment was laid out in randomized block design with seven treatment and replicated thrice. The observation larval populations of H. armigera were recorded one day before treatment was recorded at 3,7, and 10 days after treatments. The larva population of H. armigera appeared in the third week of February (8 the Standard week) and reached its peak of 14.65 larvae in first week of April and decline rapidly with maturation of crop. There was only one peak in the larval population observation in the 1st week. Bacillus thuringiensis was the most effective chemical by D.D.V.P.76%@0.05% . Among the microbial insecticides. HaNPV ,was the most effective followed by HaNPV+Bt and neem cake . The combination treatments were less effective than the individual treatment neem oil and B. bassiana were the least effective treatment in reducing the larval population of Heliverpa armigera.

scholarly journals Bombyx mori Pupae Efficiently Produce Recombinant AAV2/HBoV1 Vectors with a Bombyx mori Nuclear Polyhedrosis Virus Expression System

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 704
Author(s):  
Qian Yu ◽  
Pengfei Chang ◽  
Xiaoxuan Liu ◽  
Peng Lü ◽  
Qi Tang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Bombyx Mori ◽  
Nuclear Polyhedrosis Virus ◽  
Expression System ◽  
Efficient Production ◽  
Human Bocavirus ◽  
Adeno Associated Virus ◽  
Silkworm Pupae ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

Recombinant adeno-associated virus (AAV) vectors have broad application prospects in the field of gene therapy. The establishment of low-cost and large-scale manufacturing is now the general agenda for industry. The baculovirus-insect cell/larva expression system has great potential for these applications due to its scalability and predictable biosafety. To establish a more efficient production system, Bombyx mori pupae were used as a new platform and infected with recombinant Bombyx mori nuclear polyhedrosis virus (BmNPV). The production of a chimeric recombinant adeno-associated virus (rAAV) serotype 2/human bocavirus type-1 (HBoV1) vector was used to evaluate the efficiency of this new baculovirus expression vector (BEV)–insect expression system. For this purpose, we constructed two recombinant BmNPVs, which were named rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP. The yields of rAAV2/HBoV1 derived from the rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP co-infected BmN cells exceeded 2 × 104 vector genomes (VG) per cell. The rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP can express stably for at least five passages. Significantly, rAAV2/HBoV1 could be efficiently generated from BmNPV-infected silkworm larvae and pupae at average yields of 2.52 × 1012 VG/larva and 4.6 × 1012 VG/pupa, respectively. However, the vectors produced from the larvae and pupae had a high percentage of empty particles, which suggests that further optimization is required for this platform in the future. Our work shows that silkworm pupae, as an efficient bioreactor, have great potential for application in the production of gene therapy vectors.

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