scholarly journals Inhibición de Streptococcus mutans aislado de cavidad oral de niños sin caries mediante sustancia antagónica producida por Lactobacillus spp.

2021 ◽  
Vol 10 (1) ◽  
pp. 11
Author(s):  
Willy Bustillos Torrez ◽  
Zulema Susy Bueno Bravo

La caries es considerada todavía una de las enfermedades más prevalentes del mundo. Por este motivo, diversas estrategias de prevención se han desarrollado en los últimos años con el propósito de controlar esta enfermedad. Diferentes especies bacterianas de la microbiota normal de la cavidad oral producen sustancias antagónicas antibacterianas. Objetivo: El objetivo de este trabajo fue identificar alguna especie del género Lactobacillus productor de sustancias antagónicas contra Streptococcus mutans a partir de saliva de niños con y sin caries. Materiales y métodos: Se aislaron desde saliva de 60 niños con caries, sin caries activa (rehabilitados) y libres de caries, diferentes cepas de Lactobacillus a las cuales se les estudió su capacidad antagónica contra cepas de Streptococcus mutans , mediante ensayos en doble capa, test del pocillo y sobre crecimiento bacteriano. Resultados: Las cepas que elaboran sustancias con mayor capacidad antagónica fueron identificadas como Lactobacillus fermentum mediante Api test 50 CH. Conclusión: Se demostró que Lactobacillus fermentum está presente en mayor porcentaje en el grupo de niños sin caries, lo cual podría sugerir un efecto natural de control biológico en la cavidad oral de este grupo de niños.

Jurnal Jeumpa ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 614-624
Author(s):  
Milfa Aini ◽  
Sri Rahayuni ◽  
Vivi Mardina ◽  
Quranayati Quranayati ◽  
Nur Asiah

Kelompok BAL (Bakteri Asam Laktat) yaitu berasal dari genus Lactobacillus  terdiri dari hampir 80 spesies berbeda. Jenis Lactobacillus terdiri dari dua kelompok yang bersifat heterofermentatif dan homofermentatif. Spesies dari bakteri Lactobacillus yang tergolong homofermentatif adalah Lactobacillus bulgaricus, Lactobacillus thermophiles, Lactobacillus lactis, dan Lactobacillus acidophilu. Spesies bakteri yang bersifat heterofermentatif adalah Lactobacillus Fermentum. Bakteri Asam Laktat (BAL) adalah bakteri yang digunakan sebagai probiotik. Genus Lactobacillus terdiri atas banyak spesies yang digunakan untuk fermentasi dan pengawet makanan. Ada 106 spesies Lactobacillus, namun hanya 56 spesies berpotensi sebagai probiotik. Penelitian ini menggunakan metode adalah deskriptif restrospektif. Sampel penelitian berupa hasil literature dari jurnal dan penelitian yang telah diterbitkan. Tujuan penelitian ini adalah mereview berbagai jenis bakteri Lactobacillus spp dan perananya untuk kehidupan.


2019 ◽  
Vol 43 (4) ◽  
pp. 239-243 ◽  
Author(s):  
Aline dos Santos Letieri ◽  
Liana Bastos Freitas-Fernandes ◽  
Ana Paula Canedo Valente ◽  
Tatiana Kelly da Silva Fidalgo ◽  
Ivete Pomarico Ribeiro de Souza

Background: Our aim was to compare salivary levels of secretory immunoglobulin A (s-IgA) in children with early childhood caries (ECCG) and those who are caries-free (CFG) and verify these levels in a follow-up period after restorative treatment. Materials and methods: We selected 46 systemically healthy children in the complete primary dentition period, who were allocated into two groups: CFG (n = 23) and ECCG (dmf-s > 0; n = 23). Unstimulated whole saliva was obtained at baseline from both groups and during the follow-up period (7 days, 1, 2 and 3 months) in the ECCG group. The s-IgA was measured using an ELISA assay, and total protein was assessed using the Bradford method. We also evaluated the flow rate (mL/min), Streptococcus mutans and Lactobacillus spp. counting using selective media plaques. The data were submitted to statistical analysis using the software SPSS 20.0 (SPSS Inc, IL, USA) with a confidence interval set at 95%. Results: Salivary s-IgA levels were higher in baseline of ECCG than in CFG (p<0.05). No statistically significant differences were observed between s-IgA salivary levels at baseline and the evaluations after dental treatment in ECCG (p>0.05). However, we observed two different changes in s-IgA levels among participants: one group presented s-IgA reduction, and the other group demonstrated its maintenance. It was shown that patients from the ECCG group who presented a reduction in s-IgA levels during follow-up also showed a decrease in Streptococcus mutans and Lactobacillus spp. count (p<0.05), in contrast to patients who did not present this reduction. The flow rate and total protein were similar between groups (p>0.05). Conclusions: The present data support the idea that children with early childhood caries present higher levels of s-IgA in saliva than caries-free children. The restorative dental treatment does not have a significant influence on salivary levels of this immunoglobulin during the follow-up period.


1983 ◽  
Vol 62 (6) ◽  
pp. 746-751 ◽  
Author(s):  
L.R. Brown ◽  
J.O. White ◽  
I.M. Horton ◽  
S. Dreizen ◽  
J.L. Streckfuss

Twelve consecutive wk of daily five-minute topical applications of 1% NaF gel by non-cancer control subjects did not significantly affect plaque concentrations of Streptococcus mutans or Lactobacillus spp. Plaque F- levels increased 150% (P <.001), while production of acetate and lactate decreased 40% (P < .007) and 66% (P < .001), respectively. Long-term (12 wk to more than five yr) fluoride gel use by post-irradiation xerostomic cancer patients was associated with increases in plaque F- and decreases in acidogenesis similar to those observed in the control subjects. Plaque concentrations of cariogenic organisms increased during the first yr of radiation-induced xerostomia and fluoride gel use, before starting to decline. Although sustained fluoride treatment increased (P <.001) the ratio of fluoride-resistant to fluoride-sensitive strains, the number of patients harboring detectable S. mutans was diminished (P < .001).


2010 ◽  
Vol 5 (3) ◽  
pp. 144-149 ◽  
Author(s):  
Dilsah Cogulu ◽  
Asli Topaloglu-Ak ◽  
Esber Caglar ◽  
Nuket Sandalli ◽  
Cem Karagozlu ◽  
...  

2020 ◽  
Vol 148 (5-6) ◽  
pp. 270-274
Author(s):  
Tanja Ivanovic ◽  
Ljiljana Stojanovic ◽  
Dragan Ivanovic ◽  
Predrag Nikolic ◽  
Zeljko Milosavljevic ◽  
...  

Introduction/Objective. Malocclusions are one of the most frequent disorders in dentistry, and pose a risk for the onset of caries and periodontal diseases. Fixed orthodontic treatment solves the problem of malocclusions; however, it requires simultaneous cooperation of the patients, parents, and dentists involved. The objective of this study is to examine the effects of fixed orthodontic therapy on the Streptococcus mutans and Lactobacillus spp. bacteria in saliva, the pH value, and buffering capacity of saliva. Methods. The research was carried out at the Faculty of Medicine in Foca, Department of Dentistry. The study included 100 respondents, aged 13 to 17 years. The respondents were divided into two groups: the study group (respondents wearing fixed braces) and the control group (respondents not subjected to fixed braces therapy). Saliva samples were taken from the respondents four, 12, and 18 weeks after the start of the orthodontic therapy. The study used the bacteria caries risk test (CRT) and CRT buffer (Ivoclar Vivadent). Results. The study showed an increased number of bacteria in saliva of the respondents during all three follow-up periods (?2 test, p = 0.001). The largest numbers of the Streptococcus mutans and Lactobacillus spp. bacteria were found in week 12 of the therapy. Saliva pH value and buffering capacity of saliva increased statistically significantly in week 12 of the therapy (?2 test, p = 0.001). Conclusion. Oral conditions in patients changed during the fixed orthodontic therapy: the number of bacteria increased, the pH value and buffering capacity of saliva changed. It was necessary to use preventive measures in order to avoid complications during the fixed orthodontic appliances therapy.


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