Solved problems of reproductive medicine in the Czech Republic 2020

2021 ◽  
Vol 86 (2) ◽  
pp. 140-147
Author(s):  
Pavel Ventruba ◽  
◽  
Jana Žáková ◽  
Karel Řežábek ◽  
Kateřina Veselá ◽  
...  
Keyword(s):  
Czech Republic ◽  
Genome Editing ◽  
Assisted Reproduction ◽  
Reproductive Medicine ◽  
In Vitro Fertilisation ◽  
The Czech Republic ◽  
Non Invasive ◽  
Oncogenic Mutations ◽  
Patients At Risk

Overview Introduction: During the 30th symposium of assisted reproduction held on November 11, 2020 in Brno, the solved problems in reproductive medicine in the Czech Republic in 2020 were presented. The selected topics have concerned not only current issues in the field of clinical embryology and genetics as well as gynecology, but also legislation and ethics. Discussed topics: 1. How much time does the doctor have in the CAR (centrum of assisted reproduction) outpatient clinic per patient and how does the embryologist communicate with clients? 2. Reproduction and PGT-M in oncology patients and patients at risk with hereditary oncogenic mutations. 3. Non-invasive genetic testing of embryos from culture medium. 4. Genome editing. 5. What is the need to monitor hormonal levels in stimulation protocols? 6. Monitoring and embryo selection for transfer/kryo. 7. Is it time to change the law on donor remuneration? Methods: The topics were prepared in advance by authorized members of our company with the task of elaborating theses, which they presented in a separate conference block. The presentation and the discussion were broadcast directly from the broadcast studio at Hotel International via an online connection. After the conference, all discussion topics and comments were incorporated. Conclusion: The work presents the state of the solved problems of reproductive medicine in the Czech Republic. Keywords: current topics – assisted reproduction – in vitro fertilisation – reproductive medicine – PGT – genome editing – embryo monitoring

Qualitative research in reproductive medicine: From description to action

Human Affairs ◽  
2012 ◽  
Vol 22 (4) ◽  
Author(s):  
Hana Konečná ◽  
Tonko Mardešić ◽  
Taťána Rumpíková ◽  
Tomáš Kučera
Keyword(s):  
Qualitative Research ◽  
Czech Republic ◽  
Assisted Reproduction ◽  
Reproductive Medicine ◽  
Structural Changes ◽  
International Communication ◽  
Research Project ◽  
The Czech Republic

AbstractAssisted reproduction (ART), particularly that performed using donated gametes, increases the prospect of healthy babies being delivered to increasing numbers of people striving for parenthood. The psychosocial, ethical and legislative issues related both to the donation and receipt of gametes are perceived as extraordinarily complicated. In 2009, a research project aimed at mapping the issues was drawn up and implemented in the Czech Republic. The project should have provided material for consultation purposes, for the work of ethical and legislative bodies, and for better interdisciplinary and international communication in reproductive medicine. Work on the project was affected by several unforeseen events, particularly by the drafting and adoption of a new law on ART (to which the project was initially to have contributed material once concluded). The article describes the dynamic and structural changes occurring within the project due to drafting of the bill as well as the changes and consequences resulting from other circumstances related to the topic researched.

scholarly journals Menstrual flow as a non-invasive source of endometrial organoids

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Tereza Cindrova-Davies ◽  
Xiaohui Zhao ◽  
Kay Elder ◽  
Carolyn J. P. Jones ◽  
Ashley Moffett ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Recurrent Miscarriage ◽  
Milk Proteins ◽  
Receptor Expression ◽  
In Vitro Fertilisation ◽  
Non Invasive ◽  
Menstrual Flow ◽  
Pregnancy Hormones

AbstractAssessment of the endometrium often necessitates a biopsy, which currently involves an invasive, transcervical procedure. Here, we present an alternative technique based on deriving organoids from menstrual flow. We demonstrate that organoids can be derived from gland fragments recovered from menstrual flow. To confirm they faithfully reflect the in vivo state we compared organoids derived from paired scratch biopsies and ensuing menstrual flow from patients undergoing in vitro fertilisation (IVF). We demonstrate that the two sets of organoids share the same transcriptome signature, derivation efficiency and proliferation rate. Furthermore, they respond similarly to sex steroids and early-pregnancy hormones, with changes in morphology, receptor expression, and production of ‘uterine milk’ proteins that mimic those during the late-secretory phase and early pregnancy. This technique has wide-ranging impact for non-invasive investigation and personalised approaches to treatment of common gynaecological conditions, such as endometriosis, and reproductive disorders, including failed implantation after IVF and recurrent miscarriage.

Acupuncture Prior to and at Embryo Transfer in An Assisted Conception Unit – a Case Series

2006 ◽  
Vol 24 (1) ◽  
pp. 23-28 ◽  
Author(s):  
David Johnson
Keyword(s):  
Success Rate ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Assisted Reproduction ◽  
Case Series ◽  
In Vitro Fertilisation ◽  
Assisted Conception ◽  
Before And After ◽  
In Pregnancy

Over a period of three years, acupuncture was offered to patients entering assisted reproduction therapy. Acupuncture sessions were given at varying, but usually weekly, intervals during the in vitro fertilisation (IVF) cycle, and immediately before and after embryo transfer. Twenty two patients (average age 36.2 years) were treated over a total of 26 IVF cycles and 15 pregnancies were achieved, as determined by presence of foetal heartbeat on ultrasound at four weeks post embryo transfer. This was a success rate of 57.7% compared with 45.3% for patients in the IVF unit not treated with acupuncture (P>0.05). Relaxing effects were noted following acupuncture and it is speculated that this may have contributed to the increase in pregnancy rate for the acupuncture group.

scholarly journals The Evolution of Legislation in the Field of Medically Assisted Reproduction and Embryo Stem Cell Research in European Union Members

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Francesco Paolo Busardò ◽  
Matteo Gulino ◽  
Simona Napoletano ◽  
Simona Zaami ◽  
Paola Frati
Keyword(s):  
Assisted Reproduction ◽  
Embryo Research ◽  
In Vitro Fertilisation ◽  
Medical Field ◽  
Medically Assisted Reproduction ◽  
Eu Countries ◽  
Eu Member States ◽  
Regulatory Approach ◽  
Different Origins

Medically Assisted Reproduction (MAR), involving in vitro fertilisation (IVF), and research on embryos have created expectation to many people affected by infertility; at the same time it has generated a surplus of laws and ethical and social debates. Undoubtedly, MAR represents a rather new medical field and constant developments in medicine and new opportunities continue to defy the attempt to respond to those questions. In this paper, the authors reviewed the current legislation in the 28 EU member states trying to evaluate the different legislation paths adopted over the last 15 years and highlighting those EU countries with no specific legislation in place and MAR is covered by a general health Law and those countries in which there are no laws in this field but only “guidelines.” The second aim of this work has been to compare MAR legislation and embryo research in EU countries, which derive from different origins ranging from an extremely prohibitive approach versus a liberal one, going through a cautious regulatory approach.

scholarly journals Resistance behaviour of Septoria tritici to some fungicides in the territory of the Czech Republic

2012 ◽  
Vol 58 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Ludvik Tvaružek ◽  
P. Horáková ◽  
L. Ji
Keyword(s):  
Czech Republic ◽  
Septoria Tritici ◽  
Resistance Development ◽  
The Czech Republic ◽  
Colony Diameter ◽  
Dmi Fungicides ◽  
The Mean ◽  
Different Parts ◽  
Pathogen Strains

Reduction of colony diameter using two DMI-fungicides and one strobilurine fungicide was evaluated in the <i>Septoria tritici</i> population. In an <i>in vitro</i> assay, 57 strains of <i>S. tritici</i> originating from different parts of the Czech Republic were grown on agar plates containing different concentrations of fungicides. The mean EC<sub>50</sub> values of strains were analysed for flusilazole (Capitain), metconazole (Caramba) and one strobilurine fungicide: azoxystrobin (Amistar). ED 50 values for metconazole were low. There were highly significant correlation in reaction of pathogen strains to metconazole and flusilazole. Some isolates showed significant higher level of resistance to azoxystrobin. The data will be used in a continuing survey of resistance development in the <i>S. tritici</i> population originating from the territory of the Czech Republic.

scholarly journals Catalogue of alien animal species in the Czech Republic

2005 ◽  
Vol 53 (4) ◽  
pp. 151-170 ◽  
Author(s):  
Hana Šefrová ◽  
Zdeněk Laštůvka
Keyword(s):  
Czech Republic ◽  
North America ◽  
Animal Species ◽  
Stored Products ◽  
Local Fauna ◽  
Forest Pests ◽  
The Czech Republic ◽  
Non Invasive ◽  
Stored Product Pest ◽  
Invasive Status

The catalogue of alien animal species registered in the Czech Republic, with data on their origin, date on the first observation, way of introduction (accidental, deliberate, spontaneous), invasive status (casual, non-invasive, post-invasive, invasive), habitat (eusynanthropic, urban, agricultural, natural), trophic requirements and possible influences (plant or stored-product pest, biodiversity influence). In total 595 species are listed, i.e. 1.8% of the fauna of this country; of these, 22 species of molluscs (8.8% of the local fauna), 451 spp. of arthropods (1.5%), 383 spp. of insects (1.4%), and 55 spp. of vertebrates (9.2%). Among the registered species, 248 spp. (41.8%) are confined to closed and heated spaces by their occurrence, and 287 spp. have become naturalized (48.2%). Of these 113 spp. are considered invasive (19% of alien spp.). 65 spp. (10.9% of aliens) are pests of stored products, 84 spp. (14.1%) are parasites of important animals, 53 spp. (8.9%) are pests of plants grown in heated rooms (above all, glasshouses), 28 ssp. (4.7%) are agricultural or forest pests, and 39 spp. (6.6%) may influence local biodiversity. The origin of the naturalized alien species is mostly in North America (70; 24.4%), the Mediterranean (61; 21.3%), E Asia (44; 15.4%), Central and SW Asia (43; 15%), and S or SE Asia (30; 10.5%).

scholarly journals Introduced and invasive insect species in the Czech Republic and their economic and ecological impact (Insecta)

2005 ◽  
Vol 53 (5) ◽  
pp. 151-158 ◽  
Author(s):  
Hana Šefrová
Keyword(s):  
Invasive Species ◽  
Czech Republic ◽  
Ecological Impact ◽  
Outdoor Environment ◽  
Insect Species ◽  
Local Fauna ◽  
The Czech Republic ◽  
Non Invasive ◽  
Alien Insect ◽  
Taxonomic Groups

A total of 383 alien insect species were registered in the Czech Republic, which represents 1.4% of local fauna. The most numerous taxonomic groups are Homoptera (116 species, 30.3%), Coleoptera (110; 28.7%) and Lepidoptera (37; 9.7%). The occurrence of 200 species (52.2%) are limited to closed heated spaces, casual aliens (28; 7.3%) infiltrate the outdoor environment for a short term only, 36 (9.4%) naturalized non-invasive species do not spread from the location of introduction, 50 (13.1%) species are post-invasive and 69 (18.0%) invasive. From the species registered, 61 (15.9%) are stored product pests (especially Coleoptera 36 species, Psocoptera 11, and Lepidoptera 9), 50 (13.1%) are plant pests indoors (especially Coccinea 33 species, Aphidinea 7, and Thysanoptera 6), 25 (i.e. 6.5% of aliens) are pests in agriculture, forestry, and in ornamental cultures, 15 species (3.9%) are important animal parasites, and 5 species (1.3%) can affect biodiversity. Of the remaining 227 species (59.3%), no economic or ecological effects were found. The origin of most of the species living eusynanthropically is in the tropics and subtropics; of the 155 naturalized (non-invasive, post-invasive, and invasive) species, 42 (27.1%) originate from the Mediterranean, 36 (23.2%) from North America, 28 (18.1%) from Central to Southwest Asia, 14 (9.0%) from East Asia, 13 (8.4%) from South and Southeast Asia, with the remaining 22 species (14.2%) coming from other areas.

Cohabitation and Assisted Reproduction in the Czech Republic and in the European Context

2021 ◽  
Author(s):  
Denisa Kotroušová
Keyword(s):  
Czech Republic ◽  
Assisted Reproduction ◽  
Legal Regulation ◽  
European Countries ◽  
The Czech Republic ◽  
European Context

The contribution deals with a rather narrow topic related to cohabitation – namely with the access of cohabitants to assisted reproduction (ART). The focus is being put primarily on the Czech legal regulation of this issue. Then there is a broader European context added in the contribution. Some European countries are analysed in greater detail, especially if their approach to ART is specific or interesting. In the end, there is reflection of the Czech approach, taking into consideration the differences between the Czech Republic and other European countries.

Abstract #1003231: Genetic Hypertriglyceridemia [HTG], Assisted Reproduction – In-Vitro Fertilisation [IVF] and Acute Pancreatitis [AP]

2021 ◽  
Vol 27 (6) ◽  
pp. S136-S137
Author(s):  
Mandyam D. Chitra ◽  
Jayalakshmi Thimmaiah ◽  
Apoorva Govind ◽  
siddhartha dinesha ◽  
Prateek Hongal ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Assisted Reproduction ◽  
In Vitro Fertilisation

scholarly journals Protocol for efficient micropropagation of spring gentian and sand jurinea

2014 ◽  
Vol 60 (No. 1) ◽  
pp. 1-5
Author(s):  
H. Cvrčková ◽  
P. Máchová ◽  
J. Dostál ◽  
J. Malá
Keyword(s):  
Czech Republic ◽  
Plant Species ◽  
In Vitro Propagation ◽  
Casein Hydrolysate ◽  
Endangered Plant ◽  
Ms Medium ◽  
The Czech Republic ◽  
Endangered Plant Species ◽  
Primary Explants

Protocols for the in vitro propagation of two endangered plant species in the Czech Republic, Gentiana verna L. and Jurinea cyanoides (L.) Rchb., were established. In G. verna, the induction of organogenesis on vegetative shoots was successful on the basal 6% agar WPM medium with 200 mg&middot;l<sup>&ndash;1</sup>of glutamine, 200 mg&middot;l<sup>&ndash;1 </sup>of casein hydrolysate, 30 g&middot;l<sup>&ndash;1 </sup>of sucrose, 0.2 mg&middot;l<sup>&ndash;1 </sup>of BAP, and 0.1 mg&middot;l<sup>&ndash;1 </sup>of IBA. The multiplication of primary explants was achieved on 6% agar basal MS medium with the same concentrations of substances mentioned above. In J. cyanoides, the induction of organogenesis and multiplication of its vegetative shoots were successful on MS medium with the same concentration of added substances used in G. verna. On the basal MS medium of 1/3 concentration with 3 mg&middot;l<sup>&ndash;1 </sup>of IBA 70&ndash;75% rooting efficiency of G. verna microcuttings and 40% rooting efficiency of J. cyanoides microcuttings were reached. The mortality during acclimatization did not exceed 20% for G. verna and 25% for J. cyanoides. &nbsp; &nbsp;

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