Microbial Leakage of Cavit, IRM, and Temp Bond in Post-prepared Root Canals Using Two Methods of Gutta-percha Removal: An In Vitro Study

2005 ◽  
Vol 6 (3) ◽  
pp. 53-61 ◽  
Author(s):  
Hanan Balto ◽  
Saad Al-Nazhan ◽  
Khulood Al-Mansour ◽  
Moneera Al-Otaibi ◽  
Yunus Siddiqu
Keyword(s):  
Root Canal ◽  
Bacterial Suspension ◽  
Control Group ◽  
Endodontically Treated Teeth ◽  
Root Canals ◽  
Gutta Percha ◽  
Working Length ◽  
Significant Difference ◽  
Post Space

Abstract The aim of this study was to evaluate the integrity of the coronal seal of Temp-Bond and compare it to Cavit and IRM after post space preparation using S. faecalis as a microbial tracer. In addition, the affect of two methods of gutta percha removal on the apical seal of root canal fillings was also evaluated. Forty extracted human single rooted teeth were prepared chemomechanically and obturated with gutta percha and AH26 sealer cement using the lateral cold condensation technique to a standardized working length of 15 mm. About 10 mm of the coronal gutta-percha was removed with either Peeso-reamer or a hot plugger. The roots were divided into three experimental groups of 10 roots and a control group. Each experimental group was subdivided equally into two groups of 15 each according to the method of post space preparation. Cavit, IRM, and Temp-Bond were used to seal the access opening. Each root was fixed in a cuvette containing Tryptic Soya Broth which, covered 2 mm of the root apex. Bacterial suspension was introduced through pipette. Fresh bacterial suspension was added every week, and the system was monitored daily for the growth of microorganisms for a period of one month. The results showed there was no significant difference in terms of coronal leakage between the three coronal materials used (P=0.478), but the methods of gutta-percha removal did have an impact on the apical leakage (P=0.047). The mean value showed the Peeso-reamer provided less leakage compared to using a hot plugger during the 30-day experimental time period. It was concluded the temporary type of coronal seal of endodontically treated teeth will not prevent coronal leakage if left for a long period of time. In addition, permanent cementation of the post with the coronal restoration should be carried out as soon as possible to prevent recontamination of the root canal. Citation Balto H, Al-Nazhan S, Al-Mansour K, Al-Otaibi M, Siddiqu Y. Microbial Leakage of Cavit, IRM, and Temp Bond in Post-prepared Root Canals Using Two Methods of Gutta-percha Removal: An In Vitro Study. J Contemp Dent Pract 2005 August;(6)3:053-061.

In vitro Comparative Evaluation of the Fracture Resistance of Simulated Immature Teeth reinforced with Different Apical Barriers and Obturation Combination

2016 ◽  
Vol 7 (3) ◽  
pp. 113-118
Author(s):  
Neveen A Shaheen ◽  
Nahla G El-Din El-Helbawy
Keyword(s):  
Fracture Resistance ◽  
Comparative Evaluation ◽  
Negative Control ◽  
Control Group ◽  
Root Canals ◽  
Immature Teeth ◽  
Gutta Percha ◽  
Group I ◽  
Significant Difference

ABSTRACT Aim This study aimed to assess and compare the fracture resistance of simulated immature teeth reinforced with Biodentine (BD) and mineral trioxide aggregate (MTA) as apical barriers and two root canal backfilling combination (gutta-percha/AH26, MetaSeal). Materials and methods A total of 70 extracted human maxillary incisors were randomly divided into seven groups (n = 10). The positive control group was not instrumented. For the other groups, coronal access was made and root canals were instrumented using the ProTaper, up to F5 followed by six Peeso reamers which were allowed to pass 1 mm beyond the apex to size 6 (1.7 mm) to simulate immature teeth. The apical 4 mm of their root canals was filled with either MTA or BD apical barrier, then backfilled with gutta-percha/AH26 or MetaSeal obturation combination. The negative control group was left unfilled. Composite resin was used to restore the coronal access cavities. The maximum load for fracture of each tooth was recorded utilizing a universal testing machine. Data were analyzed using two-way analysis of variance. Results The noninstrumented group I had the highest fracture resistance and differed significantly (p < 0.05) from the negative control groups. On the contrary, no significant difference was found between BD and MTA groups, regardless of the backfilling combination (p > 0.05). Conclusion There was no difference between MTA and BD apical barriers and the backfilling combination regarding their resistance to root fracture. How to cite this article Shaheen NA, El-Din El-Helbawy NG. In vitro Comparative Evaluation of the Fracture Resistance of Simulated Immature Teeth reinforced with Different Apical Barriers and Obturation Combination. World J Dent 2016;7(3):113-118.

scholarly journals Evaluation of the apical microleakage of MTA Fillapex, AH26, and Endofill sealers

2020 ◽  
Vol 23 (3) ◽  
Author(s):  
Saeedeh Galledar ◽  
Robab Farhang ◽  
Malek Abazari ◽  
Parisa Negahdar
Keyword(s):  
Root Canal ◽  
Test Group ◽  
In Vitro Study ◽  
Negative Control ◽  
Vitro Study ◽  
Control Group ◽  
Dye Penetration ◽  
Gutta Percha ◽  
Significant Difference

Objective: Proper apical seal plays an important role in the success of root canal treatment. The most common cause of failure of root canal therapy is known as the lack of adequate apical seal. The aim of this in vitro study was to compare the apical microleakage of MTA Fillapex, , and Endofill sealers using dye penetration method. Material and Methods: In this in vitro study, 72 single-rooted extracted human teeth were selected. The teeth were randomly divided into three experimental groups of 20 and two positive and negative control groups of 6. The canals were prepared by step-back technique and then filled with gutta-percha and one of the sealers mentioned. In the positive control group, the canals were filled with gutta-percha without sealer, and in the negative control group, the canals were prepared but not filled. The teeth were immersed in 2% methylene blue dye for 72 hours. The teeth were then cut longitudinally and the level of dye penetration was measured under a stereomicroscope. Data were analyzed by SPSS ver. 19 software, ANOVA and Bonferroni post-hoc tests. Results: The mean level of dye penetration in the Endofill test group was significantly higher than that in the  and MTA Fillapex test groups. While, the observed difference between  and MTA Fillapex groups was not statistically significant (p<0.05). Conclusion: The results of this study showed that  and MTA Fillapex sealers did not show any significant difference in apical seal properties. However, their sealing strength was significantly greater than Endofill sealer.KeywordsAH26 sealer; Endofill; MTA Fillapex; Microleakag

scholarly journals The influence of working length on the reduction of biofilm and planktonic bacteria in oval canals with reciprocating instrumentation

2021 ◽  
Vol 10 (5) ◽  
pp. e9810514765
Author(s):  
Arieth Cristina Sacomani ◽  
Fernanda Tessaro Cintra ◽  
Adriana de Jesus Soares ◽  
Marcos Frozoni
Keyword(s):  
Bacterial Growth ◽  
Root Canal ◽  
Negative Control ◽  
Bacterial Biofilm ◽  
Control Group ◽  
Root Canal Preparation ◽  
Root Canals ◽  
Planktonic Bacteria ◽  
Working Length ◽  
Significant Difference

To evaluate the influence of reciprocating single-file instrumentation with different working lengths (WL) on the reduction of planktonic bacteria and bacterial biofilm in Enterococcus faecalis-contaminated oval root canals. Methodology: Fifty-five human single-rooted canines were used. Fifty were inoculated with E. faecalis for 21 days for biofilm formation. To confirm the formation of biofilm adhered to the root canal wall, 5 contaminated samples from positive control group were analyzed by SEM. Samples were assigned into 3 groups (n = 15) according to working length determined, G+1 root canal preparation 1 mm beyond the apical foramen, G0 root canal preparation at the major foramen, and G-1 root canal preparation 1 mm short of the major foramen. Five roots were not inoculated to serve as a negative control. Bacteriological samples were collected prior to preparation, initial collection (S1), and after reciprocating instrumentation (S2) by disaggregating biofilm to quantify the reduction of planktonic bacteria and intracanal biofilm at different WL. Bacterial quantitation was performed using colony-forming units per milliliter (CFU / mL) count. Statistical analysis was performed at the significance level of 0.05. Results: No bacterial growth was observed in the negative control. All positive controls demonstrated bacterial growth; S1 from all teeth were positive for bacteria with no significant difference. The post-hoc analysis showed G+1 promoting a significantly higher disinfection than G-1 (p<0,05) and G-1 similar disinfection to G0 (P=962). Conclusion: Instrumentation as close as possible to major foramen or beyond it improves decontamination in oval root canals with reciprocating instrumentation.

scholarly journals Comparative study of coronal sealing ability using root cross sections method

2007 ◽  
Vol 54 (4) ◽  
pp. 224-230
Author(s):  
Dejan Markovic ◽  
Vesna Zivojinovic ◽  
Slavoljub Zivkovic
Keyword(s):  
Cross Sections ◽  
Root Canal ◽  
Smear Layer ◽  
Blue Dye ◽  
Human Teeth ◽  
Root Canals ◽  
Dye Penetration ◽  
Gutta Percha ◽  
Significant Difference

Objective: An in vitro comparison of coronal microleakage of three root canal sealers was performed using the dye leakage method. Methodology: Ninety-eight single-rooted human teeth were instrumented and randomly allocated to two groups. In the first group, root canals were treated with EDTA to remove the smear layer. The roots in both groups were obturated using laterally condensed gutta-percha and Sealapex, AH-26 or Ketac-Endo as the sealer. The teeth were suspended in 1% methylene blue dye for 6 days. The teeth were then rinsed, sectioned perpendicular to the long axis and evaluated for coronal dye penetration. Results: In general, Ketac-Endo root canal sealer showed greater dye penetration than Sealapex and AH-26. A statistically significant difference in the coronal penetration of the dye was found in the group with intact smear layer. Conclusion: Highly significant differences between AH-26 and Ketac-Endo (p < 0.001) and Sealapex and Ketac Endo (p < 0.001) were found.

scholarly journals Comparison of Antibacterial Effects of Photodynamic Therapy, Modified Triple Antibiotic Paste and Calcium Hydroxide on Root Canals Infected With Enterococcus faecalis: An In Vitro Study

2019 ◽  
Vol 10 (5) ◽  
pp. S23-S29 ◽  
Author(s):  
Mohammad Asnaashari ◽  
Mohammad Jafar Eghbal ◽  
Amirali Sahba Yaghmayi ◽  
Mehdi Shokri ◽  
Saranaz Azari-Marhabi
Keyword(s):  
Photodynamic Therapy ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Control Group ◽  
Root Canals ◽  
Anterior Teeth ◽  
Triple Antibiotic Paste ◽  
Working Length

Introduction: A pivotal issue to achieve success in the treatment of the root canal is root canal disinfection. One of the most important bacteria that infect the root canal is Enterococcus faecalis. This study seeks to examine the effectiveness of 3 methods for disinfecting the root canal: photodynamic therapy, modified triple antibiotic paste (MTAP), and calcium hydroxide. Methods: Sixty-two single-rooted extracted anterior teeth were collected. After cleaning and disinfecting the teeth, their crowns were cut at the CEJ point. The root canals were shaped to the working length up to file F3 ProTaper (F1, F2, F3). EDTA 17% and sodium hypochlorite 2.5% were used for 5 minutes to wash and remove the smear layer, and then the apical foramen was sealed using composite. After that, the teeth were sterilized in an autoclave at 121°C for 15 minutes. Then 10 samples were taken randomly as the negative control. The remaining samples were immersed and cultivated in a suspension containing E. faecalis for 21 days. Then the samples were divided into 5 groups: 2 positive control groups, 1 group treated with the antibiotic paste with the concentration of 1 mg/mL, 1 group treated with calcium hydroxide, and 1 group treated with photodynamic therapy. Then, to collect the biofilm, the ProTaper file F4 was used. After that, the microbial suspension was provided and counting the colonies was carried out to compare the groups. Results: The findings indicated that the amount of CFU/mg of MTAP samples, including clindamycin, metronidazole, ciprofloxacin in the concentration of 1 mg/mL and photodynamic therapy and calcium hydroxide was lower than that in the control group. Antibiotic paste cleansed the root canal up to 99.9%. Photodynamic therapy reduced the amount of CFU/mg to 98.8%, and calcium hydroxide reduced the amount of CFU/mg to 94.13%. Conclusion: Using photodynamic therapy causes a reduction in the biofilm and inhibits the growth of the E. faecalis bacterium. In addition, in this study, MTAP with a concentration of 1 mg/mL was used, which expunged the bacteria completely. Meanwhile, calcium hydroxide had the weakest effect of all on the E. faecalis bacterium.

scholarly journals Efficacy of different instruments for the mechanical removal of the smear layer in immediate post preparations: a comparative study

2021 ◽  
Vol 34 (2) ◽  
pp. 166-172
Author(s):  
Marcela Roitman ◽  
Laura Pinasco ◽  
Romina Loiacono ◽  
Valeria Panetta ◽  
Carolina Anaise ◽  
...  
Keyword(s):  
Root Canal ◽  
Ex Vivo ◽  
Close Contact ◽  
Smear Layer ◽  
Control Group ◽  
Endodontically Treated Teeth ◽  
Single Root ◽  
Mechanical Removal ◽  
Significant Difference ◽  
Post Space

Adhesively cemented fiber-reinforced composite posts are commonly used to reconstruct endodontically treated teeth. Adhesion to intraradicular dentin is complex, and close contact between the resin cements and the dentin is essential. The removal of the smear layer following post space preparation (secondary smear layer) can improve this integration. Different mechanical systems have been used to activate the irrigant inside the root canal. The purpose of this study was to compare, ex-vivo, the removal of the smear layer from the walls of the immediate post space preparation by the action of three mechanized instruments. Forty premolar specimens with a single root canal were selected, endodontically treated, and shaped for glass fiber post cementation with Peeso reamer #1 (Dentsply Sirona, Switzerland) and Macrolock finishing drill #3 (RTD, France). The specimens were randomly divided into 4 groups (n=10): Group C (control); Group PUI (passive ultrasonic irrigation); Group CEP (Rotoprox brush, Hager Werken, Germany) and Group XP (XP-endo Finisher, FKG Dentaire, Switzerland). Post space surfaces were cleaned with 3mL of distilled water; each specimen root split longitudinally to expose the root canals, and prepared for examination in a scanning electron microscope at magnification 350X. The results were analyzed using the Kruskal-Wallis and Friedman tests (p>0.05). RESULTS: Statistically significant differences (p <0.05) were found between Groups C and XP in all three root regions, but not between Groups C, CEP and PIU. Of these, Group CEP showed a better trend in the results of the coronary and middle thirds, without significant difference with Group XP. Although it was difficult to achieve a clean dentin surface after preparation for the fiberglass post, the XP-endo Finisher was the most efficient in removing secondary smear layer, followed by the Rotoprox conical brush.

Comparison of the Accuracy of Apex Locators with Conventional Techniques in determining the Root Canal Length in Primary Teeth

2015 ◽  
Vol 5 (2) ◽  
pp. 61-64
Author(s):  
Naveen Kumar Kommineni ◽  
P Prathyusha Simha ◽  
Vinay Chand Reddy ◽  
C Lalitha Samyuktha ◽  
Prashanth M Battepati
Keyword(s):  
Digital Radiography ◽  
Root Canal ◽  
Primary Teeth ◽  
Physiological Saline ◽  
Mean Value ◽  
Root Canals ◽  
Working Length ◽  
Significant Difference ◽  
Electronic Apex Locator

ABSTRACT Aim To compare the accuracy of apex locators with conventional techniques in determining the root canal length in primary teeth. Materials and Methods This in vitro study was conducted on twenty extracted primary incisors. After endodontic access preparation, root canals were irrigated with physiological saline solution. The access cavities were dried with cotton pellets and, the roots were dried with paper points before performing the electronic measurement. One operator determined the actual working length (AWL) of the canal and another operator measured working length using conventional F-speed radiographs, digital radiography (Dr suni plus digital radiography system, model no 900-0540) and electronic apex locator- Raypex 5 (VDW GmbH Postfach) 5th generation apex locator. The mean value of differences was obtained and statistical analysis was performed using analysis of variance (ANOVA) and the paired t-test. Results There was no statistically significant difference between tooth lengths estimate on conventional, digital and apex locators. 0.5 to 1 mm of clinically acceptable discrepancy was found between three techniques. Conclusion Although there was no significant statistical difference, electronic apex locator (EAL) has more advantage over other techniques, it is considered accurate in determining the working length in primary teeth. How to cite this article Reddy VK, Kommineni NK, Simha PP, Reddy VC, Samyuktha CL, Battepati PM. Comparison of the Accuracy of Apex Locators with Conventional Techniques in determining the Root Canal Length in Primary Teeth. J Contemp Dent 2015;5(2):61-64.

scholarly journals Comparative Analysis of Efficacy and Cleaning Ability of Hand and Rotary Devices for Gutta-Percha Removal in Root Canal Retreatment: An in vitro Study

2013 ◽  
Vol 14 (4) ◽  
pp. 635-643 ◽  
Author(s):  
Srikanth Pasari ◽  
Narender Reddy ◽  
Shilpa Reddy Admala ◽  
Sainath Dinapadu ◽  
Manoranjan P Reddy ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Root Canal ◽  
Group Iv ◽  
Frictional Heat ◽  
Root Canals ◽  
Gutta Percha ◽  
Group I ◽  
Working Length ◽  
Root Canal Retreatment

ABSTRACT Aim of the study To evaluate the efficacy and cleaning ability of Hedstrom files, and ProTaper retreatment instruments in removing gutta-percha from root canals with and without xylene as solvent. Materials and methods Sixty extracted single rooted human teeth were selected and decoronated, straight access established working length determined 1 mm short of canal, chemomechanical preparation done and obturated with guttapercha and AH plus sealer. Samples were stored for 1 week in humidifier divided into four groups of 15 teeth each. • Group I Hedstrom files without xylene. • Group II Hedstrom files with xylene. • Group III ProTaper retreatment instruments without xylene. • Group IV ProTaper retreatment instruments with xylene. and the following criteria were assessed – Time taken for initial plunge of instrument into guttapercha. – Time taken for complete removal of gutta-percha to reach working length – Ability of H files and ProTaper retreatment files with/ without xylene to remove gutta-percha in coronal, middle and apical 1/3 of canal. The teeth were grooved in labiolingual cross section, observed under a steromicroscope and scored according to gutta-percha debris left in the canal. Results were evaluated using ANOVA test and multiple comparisons done using Scheffe test. Results The least time to reach working length was found with group IV followed by groups III, II and group I respectively. Also the fastest way to remove maximum gutta-percha was group IV followed by groups III, II, and I respectively with a statistically significant difference among all groups. Apical 1/3 has more amount of remaining gutta-percha debris than middle and coronal 1/3 in all groups. The amount of gutta-percha debris in apical 1/3 was least in group IV followed by groups III, II and I respectively. Discussion The better performance of ProTaper rotary instruments has been attributed to their special flute design which tends to pull gutta-percha coronally directing it toward orifice. Also the movements of engine driven instruments produce frictional heat which plasticises gutta-percha and aids in easy removal. Apical third of root canals showed more gutta percha debris compared to coronal and middle 1/3 and has been attributed to the greater anatomic variability and difficulty of instrumentation in the apical area. The existence of deep groves and depressions on dentine walls in this apical 1/3 make them less instrumented areas as it did be difficult to direct the file against the extreme root canal wall. Conclusion The fastest technique to remove gutta-percha and the shortest time to reach working length was observed with ProTaper retreatment instruments with xylene followed by ProTaper retreatment files without xylene and Hedstrom files without xylene. After instrumentation for removal of gutta-percha, apical third was found to have more debris compared to coronal and middle 1/3 of the root canal. How to cite this article Reddy N, Admala SR, Dinapadu S, Pasari S, Reddy PM, Rao MSR. Comparative Analysis of Efficacy and Cleaning Ability of Hand and Rotary Devices for gutta-percha Removal in Root Canal Retreatment: An in vitro Study. J Contemp Dent Pract 2013;14(4):635-643.

scholarly journals To Evaluate the Effect of Presence and Absence of Smear Layer with Different Instruments and Obturation Methods on Microleakage of Root Canal-Filled Teeth: An in vitro Study

2013 ◽  
Vol 4 (2) ◽  
pp. 103-107
Author(s):  
Dinesh Shetty ◽  
Veerendra Uppin ◽  
Priyadarshini Shetty
Keyword(s):  
Root Canal ◽  
In Vitro Study ◽  
Smear Layer ◽  
Vitro Study ◽  
Electrochemical Technique ◽  
Control Group ◽  
Root Canals ◽  
Gutta Percha ◽  
Presence And Absence

ABSTRACT Aim To evaluate the effects of presence and absence of smear layer with different instruments and obturation methods on microleakage of root canal-filled teeth. Materials and methods A total of 100 extracted human maxillary central incisors with closed apices and single roots were taken for the study. The teeth were divided into six groups A to F consisting of 15 teeth each. Control group consisted of 10 teeth with five positive and five negative teeth each. Groups A, B, C and D were instrumented with rotary files and groups E and F were instumented with conventional stainless steel files. Groups A, B, C and D were flushed with 3 ml of EDTA to remove the smear layer prior to obturation. All teeth were flushed with 5% NaOCl, then obturated with AH-26 sealer with lateral condensation technique on groups C, D, E, F and injectable thermoplasticized gutta-percha obturation technique on groups A and B. Using electrochemical technique the current flow in the circuit was observed for 45 days. The magnitude of the current for each tooth was directly proportional to the extent of leakage. At the intervals of 10, 20, 30 and 45 days the groups A-F were compared to identify statistically significant differences using students t-test. Results Significantly less microleakage occurred when the smear layer was removed and when the canals were obturated with thermoplasticized gutta-percha. Canals instrumented with engine driven NiTi files exhibited less leakage than hand instrumented canals. Conclusion Rotary instrumentation of the root canals provides a superior preparation in comparison to hand instrumentation. Removal of smear layer increases the resistance of microleakage. Thermoplasticized gutta-percha obturation appears to provide a superior seal as compared to lateral condensation. How to cite this article Shetty P, Uppin V, Shetty D. To Evaluate the Effect of Presence and Absence of Smear Layer with Different Instruments and Obturation Methods on Microleakage of Root Canal-Filled Teeth: An in vitro Study. World J Dent 2013;4(2):103-107.

scholarly journals Root canal curvature as a prognostic factor influencing the diagnostic accuracy of radiographic working length determination and postoperative canal axis modification: an in vitro comparative study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Bestoon Mohammed Faraj
Keyword(s):  
Root Canal ◽  
Radiographic Images ◽  
The Real ◽  
Root Canals ◽  
Length Estimation ◽  
Working Length ◽  
Length Determination ◽  
Case Difficulty Assessment ◽  
The Impact

Abstract Background Radiographic analysis of tooth morphology is mandatory for accurate calibration of the degree of canal curvature angle and radiographic working length to its real dimensions in case difficulty assessment protocols. This study aimed to determine the impact of the degree of root canal curvature angle on maintaining the real working length and the original canal axis of prepared root canals using a reciprocating rotary instrumentation technique. Methods Radiographic image analysis was performed on 60 extracted single-rooted human premolar teeth with a moderate canal curvature (10°–25°) and severe canal curvature (26°–70°). Working length and longitudinal canal axis were determined using cone-beam computed tomography (CBCT) and digital periapical radiography. The real canal length was determined by subtracting 0.5 mm from the actual canal length. Root canals were prepared using the WaveOne Gold reciprocating file (Dentsply Maillefer, Ballaigues, Switzerland). Results There was no significant relation of the degree of canal curvature angle to the accuracy of radiographic working length estimated on CBCT and digital periapical radiographic techniques (P > 0.05). Postinstrumentation changes in the original canal axis between moderate and severe canal curvature angles, assessed on CBCT and periapical digital radiographic images were statistically non-significant (P > 0.05). Conclusions A standardized digital periapical radiographic method performed similarly to the CBCT technique near to its true working length. No significant interaction exists between the diagnostic working length estimation, postoperative root canal axis modification, and the degree of canal curvature angle, using reciprocating rotary instrumentation technique.

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