An in vitro Comparison of Antimicrobial Activity of Three Endodontic Sealers with Different Composition

2016 ◽  
Vol 17 (7) ◽  
pp. 553-556 ◽  
Author(s):  
Iti Gupta ◽  
Gurpreet Singh ◽  
Faheim MM Elshamy ◽  
Husham E Homeida ◽  
Nezar Boreak
Keyword(s):  
Antimicrobial Activity ◽  
Pearson Correlation ◽  
Antimicrobial Property ◽  
Antimicrobial Properties ◽  
Agar Diffusion Test ◽  
Intergroup Comparison ◽  
Significant Difference ◽  
Zones Of Inhibition ◽  
Endodontic Sealers

ABSTRACT Aims The aim of the study was to compare the antimicrobial property of newly introduced EndoSequence BC sealer with commonly used zinc oxide-eugenol-based sealer (Zical) and epoxy resin-based sealer (MM-Seal) against Candida albicans, Lactobacillus, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Materials and methods The agar diffusion test was done to measure the antimicrobial activity of sealers. The sealers were put in the 4 mm wells prepared in the inoculated agar plates. The agar plates were incubated at 37°C for 24 hours and the zones of inhibition that appeared was measured. Chi-square test was done to evaluate intraobserver bias for all study samples. Intergroup comparison was done for all five parameters using Pearson correlation statistical analysis. Results EndoSequence BC sealer showed maximum mean of diameter of zones of inhibition against all the microorganisms but with no statistically significant difference with other sealers. Zical did not show any zone of inhibition against the P. aeruginosa. MM-Seal did not show any inhibitory activity against the P. aeruginosa and C. albicans. Conclusion EndoSequence BC sealer showed antimicrobial activity against all the microorganisms and proved to be a better choice when compared with other two sealers. Clinical significance Antimicrobial properties of endodontic sealers are important to prevent the persistent infection of the complex root canals. EndoSequence BC sealer has been proved to be a better sealer in this aspect. How to cite this article Singh G, Elshamy FMM, Homeida HE, Boreak N, Gupta I. An in vitro Comparison of Antimicrobial Activity of Three Endodontic Sealers with Different Composition. J Contemp Dent Pract 2016;17(7):553-556.

scholarly journals In vitro evaluation of the antimicrobial activity of five root canal sealers

2004 ◽  
Vol 15 (1) ◽  
pp. 30-35 ◽  
Author(s):  
Brenda Paula Figueiredo de Almeida Gomes ◽  
José Assis Pedroso ◽  
Rogério Castilho Jacinto ◽  
Morgana Eli Vianna ◽  
Caio Cezar Randi Ferraz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Contact Method ◽  
Streptococcus Sanguis ◽  
Actinomyces Naeslundii ◽  
Agar Diffusion Test ◽  
Significant Difference ◽  
Ah Plus ◽  
Endodontic Sealers

The aim of the present study was to analyze the antimicrobial properties of five endodontic sealers: Endo Fill, Endomethasone, Endomethasone N, Sealer 26 and AH-Plus, against the following microorganisms: Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus sanguis and Actinomyces naeslundii. The sealers were tested immediately, 24 h, 48 h and 7 days after manipulation.The direct contact method through the observation of the microbial growth in liquid medium and the agar diffusion test were used to evaluate the antimicrobial properties of the sealers. The results, in both methodologies used, showed that immediately after manipulation, Endo-Fill and Endomethasone demonstrated the highest antimicrobial activity, with no statistically significant difference between them. Sealer 26 demonstrated the lowest antimicrobial activity. At all other times after manipulation, there were no statistically significant differences among all the sealers tested. In conclusion, none of the sealers totally inhibited the growth of the microorganisms. Furthermore, the antimicrobial activity of each sealer decreased with time and was dependent upon the microbial susceptibility to them.

In Vitro Antimicrobial Properties of Propolis against Caries-Associated Microorganisms

2015 ◽  
Vol 752-753 ◽  
pp. 387-390
Author(s):  
Boonyanit Thaweboon ◽  
Sroisiri Thaweboon ◽  
Rattiporn Kaypetch ◽  
Thaniya Muadcheingka
Keyword(s):  
Antimicrobial Activity ◽  
Molecular Mechanisms ◽  
Biological Activities ◽  
Oral Care ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Scientific Basis ◽  
Agar Disc ◽  
Zones Of Inhibition

Natural products have attracted much attention in recent years as a useful substance used in medicine and dental products. Propolis is a lipophilic resinous mixture collected from various plant sources by honeybees. It has been known for many biological activities including antimicrobial properties. Objective: To evaluate thein vitroantimicrobial activity of propolis against caries-associated microorganisms,Streptococcusmutans,LactobacilluscaseiandCandidaalbicans. Materials and methods: The antimicrobial activity was initially screened using agar disc diffusion and then the minimum lethal concentrations (MLCs) were determined using broth dilution technique. Chlorhexidine (0.2%) and dimethyl sulfoxide (5%) were used as positive and negative controls, respectively. Results: Propolis exhibits the zones of inhibition ranged from 15 to 18 mm forS.mutans, and from 10 to 12 mm forL.casei, but presented no activity againstC.albicans. The MLC againstS.mutanswas 2.86 mg/mL and those againstL.caseiwere 11.44 mg/mL and 12.87 mg/mL. Conclusion: Propolis shows a potent antimicrobial effect againstS.mutansandL.casei.This could provide a scientific basis to its application of usage in oral care products for the prevention of dental caries. However, further studies are necessary to evaluate other biological activities and molecular mechanisms of action as well as its cytotoxic effect.

scholarly journals Antimicrobial Activity of Two Root Canal Filling Pastes in Deciduous Teeth: an in Vitro Study

2015 ◽  
Vol 17 (1) ◽  
pp. 5
Author(s):  
Andréia Bolzan de Paula ◽  
Janaina De Cássia Orlandi Sardi ◽  
Mariana Minatel ◽  
Regina Maria Puppin-Rontani
Keyword(s):  
Antimicrobial Activity ◽  
Root Canal ◽  
Antimicrobial Property ◽  
Brain Heart Infusion ◽  
In Vitro Study ◽  
Biological Properties ◽  
Deciduous Teeth ◽  
Root Canal Filling ◽  
Agar Diffusion Test

<p>Endodontic treatment in deciduous teeth is very important so that we can preserve the primary dentition and thus promote appropriate craniofacial development. However, the use of filling pastes that have some important biological properties for the preservation of the tooth is required. The objective this study was to evaluate the in vitro antimicrobial activity of two root canal filling pastes in deciduous teeth, Vitapex® and Calcipex®, on microbial species commonly found in endodontic infections (Candida albicans, Enterococcus faecalis, Streptococcus mutans, Streptococcus sanguinis, Escherichia coli and Staphylococcus aureus), using the agar diffusion test. The experiment was carried out on BHI (Brain Heart Infusion) plates in four equidistant points, which were immediately filled with the sealers Vitapex® and Calcipex®. The<br />chlorhexidine gluconate 1% (CHX) and distilled water were used as positive and negative controls, respectively. After incubation of plates at 37 °C for 24 h, the diameter of the growth inhibition zones around the wells was measured (in millimeters) with a digital caliper with reflected light. The test was performed in triplicate and data were submitted to ANOVA and Tukey’s test (α = 0.05). Both pastes had antimicrobial activity, but Calcipex® was more efficient for most strains. The halo formed in the experiments with S. mutans and S. sanguinis was similar for both pastes. This study suggests that Calcipex® had the highest antimicrobial property with respect to the microorganisms studied.</p>

scholarly journals Comparative evaluation of antimicrobial activity of biodentine and MTA against e. faecalis an in vitro study

2016 ◽  
Vol 4 (1) ◽  
pp. 16
Author(s):  
Jerin Jose ◽  
Shoba K ◽  
Nithya Tomy ◽  
Shibu Aman
Keyword(s):  
Antimicrobial Activity ◽  
In Vitro Study ◽  
Filling Material ◽  
Repair Material ◽  
Inhibition Of Growth ◽  
Root Repair ◽  
Agar Diffusion Test ◽  
Significant Difference ◽  
Surgical Options

<p><strong>Background:</strong> Surgical endodontic therapy is the prescribed treatment of choice whenever the non-surgical options fail which includes the use of a root end filling material. E. faecalis is the most prevalent organism associated with the treatment failed cases</p><p><strong>Aim:</strong> The purpose of the present study was to evaluate and compare the antimicrobial efficacy of Biodentine and MTA as root end filling material and root repair material against E. faecalis.</p><p><strong>Settings and Design:</strong> Comparative invitro study was conducted at Tropical Institute of Ecological Sciences, Kottayam under Mahatma Gandhi University, Kottayam, Kerala.</p><p><strong>Materials and Methods:</strong> The antimicrobial activity was determined using agar diffusion test. Freshly mixed MTA and Biodentine was added to respective wells and incubated. The zone of inhibition of growth was recorded.</p><p><strong>Statistical analysis:</strong> The data analysis was performed using Students ‘t’ test</p><p><strong>Results:</strong> Statistically significant difference was found between Biodentine and MTA in against E. faecalis growth.</p><p><strong>Conclusions:</strong> Biodentine was found to have superior antibacterial activity against E. faecalis than MTA. Hence it can be a better alternative to MTA as root end filling and root repair material.</p>

An Analysis of the Antimicrobial Activity of Ten Root Canal Sealers - A Duration Based in vitro Evaluation

2008 ◽  
Vol 33 (2) ◽  
pp. 117-122 ◽  
Author(s):  
Prasanna Neelakantan ◽  
Subbarao CV
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antimicrobial Action ◽  
Agar Diffusion Test ◽  
Significant Difference ◽  
Ah Plus ◽  
Root Canal Sealers

Aim and Design: Sealers with prolonged antimicrobial activity are needed by the fact that microorganisms are commonly recovered from teeth with failed endodontic therapy. The aim of this study was to evaluate the duration of antimicrobial activity of ten root canal sealers (Apexit plus, Tubli Seal Xpress, Endoflas FS,Endomethasone, Endomethasone N, AH Plus, Epiphany, EndoRez, Ketac Endo, Roeko Seal) against Enterococcus faecalis and Candida albicans by the agar diffusion test. The zones of inhibition were examined immediately and after 24, 48, 72 hours, 5 and 7 days. Statistical analysis was done using Kruskal - Wallis test and Mann Whitney U test. Results and conclusions: Against Enterococcus faecalis, Endoflas FS showed the largest inhibitory zones immediately and 24 hours after manipulation, whereas, there was no significant difference between Endoflas FS and Endomethasone after 48 hours. Against Candida albicans, Endoflas FS performed better than the other sealers. All the sealers (except AH Plus, Epiphany and Roeko Seal) demonstrated higher antimicrobial action in the first 24 hours after manipulation The antimicrobial action of all the sealers (except AH Plus and Roeko Seal which showed no antimicrobial activity in any studied time and Epiphany which ceased to show any antimicrobial action after 24 hours) decreased significantly with time.

Evaluation of antimicrobial properties of acacia catechu (Khadir): an in-vitro studyEvaluation of antimicrobial properties of acacia catechu (Khadir): an in-vitro study

2020 ◽  
Vol 12 (2) ◽  
pp. 25-30
Author(s):  
Dr. Jyotsna R Kusugal ◽  
Dr. Swati Setty ◽  
Dr. Pradeep Agnihotri ◽  
Dr.Srinath Thakur
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Property ◽  
Antimicrobial Property ◽  
Antimicrobial Properties ◽  
Subgingival Plaque ◽  
Periodontal Pathogens ◽  
Bacteriostatic Effect ◽  
Acacia Catechu ◽  
Time Kill

Context: Acacia catechu has got antimicrobial property. The evaluation of this property against periodontal pathogens may open new doors to treatment modalities of gingivitis and periodontitis. Aims: To evaluate the antimicrobial property of Acacia catechu (Khadir) against periodontal pathogens, Porphyromonas gingivalis (Pg) and Aggregatibacter actinomycetemcomitans (Aa) in subgingival plaque samples of generalized periodontitis patients. Settings and Design: Prospective, observational, microbiological, in-vitro. Methods and Material: Subgingival plaque samples of generalized periodontitis patients were collected and the two pathogens were isolated and cultured. Herbal extract was prepared in accordance with principles of Ayurveda. Antimicrobial activity was determined by minimal inhibitory concentration (MIC) and time kill assay. Statistical analysis used: None. Results: The water extract of Acacia catechu showed antibacterial property at 100μg/ml dilution in four samples for Pg and at 0.2μg/ml dilution in one sample. Antibacterial property was noted at 0.4μg/ml dilution in four samples for Aa by MIC. Bacteriostatic effect against Pg and Aa was observed by time kill assay. Conclusions: Acacia catechu bark extract exhibited antimicrobial activity against Pg and Aa. The extract also exhibited bacteriostatic effect against Pg and Aa till two hour.

scholarly journals Antimicrobial Effects of Platelet-rich Plasma against Selected Oral and Periodontal Pathogens

2017 ◽  
Vol 66 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Reham L. Aggour ◽  
Lina Gamil
Keyword(s):  
Platelet Rich Plasma ◽  
Antimicrobial Properties ◽  
Diffusion Method ◽  
Periodontal Pathogens ◽  
Antimicrobial Effects ◽  
Significant Difference ◽  
Zones Of Inhibition ◽  
Microbial Strains ◽  
Conventional Antibiotics

Antimicrobial properties of platelet rich plasma (PRP) against various microorganisms have been recently pointed out. PRP could be an alternative to conventional antibiotics in preventing oral and periodontal infections. We examined whether PRP has in vitro antimicrobial properties against Aggregatibacter actinomycetemcomitans, Prophyromonas gingivalis, Staphylococcus aureus and Candida albicans. PRP and platelet-poor plasma (PPP) were obtained from whole blood of 10 healthy volunteers and 10 periodontitis patients. In vitro laboratory susceptibility was carried out using the modified agar diffusion method by measuring the diameters of inhibition zones on agar plates coated with selected microbial strains. Both calcium chloride (CaCl2) activated and non activated samples were tested. Both activated PRP and PPP, of both patients and controls, effectively inhibited the growth of A. actinomycetemcomitans, P. gingivalis and C. albicans. However, a statistically significant difference in favor of PRP was found indicating more susceptibility to PRP than PPP (p < 0.05). Non activated PRP and PPP exhibited negative zones of inhibition against the studied microorganisms. There was no activity against S. aureus. No statistically significant difference was found between the antimicrobial effects of PRP and/or PPP obtained from patients and controls (p > 0.05). We conclude that PRP is a potentially useful substance against oral and periodontal pathogens. Activated PRP was found to be more active than activated PPP and the activation of coagulation is a fundamental step. Additionally, the antimicrobial activity of PRP and/or PPP seems not to be affected by periodontitis.

scholarly journals An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4383
Author(s):  
Barbara Lapinska ◽  
Aleksandra Szram ◽  
Beata Zarzycka ◽  
Janina Grzegorczyk ◽  
Louis Hardan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Resin Composite ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Diffusion Method ◽  
Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

scholarly journals In vitro antimicrobial activity of endodontic sealers, MTA-based cements and Portland cement

2007 ◽  
Vol 49 (1) ◽  
pp. 41-45 ◽  
Author(s):  
Mário Tanomaru-Filho ◽  
Juliane M. G. Tanomaru ◽  
Danilo B. Barros ◽  
Evandro Watanabe ◽  
Izabel Y. Ito
Keyword(s):  
Antimicrobial Activity ◽  
Portland Cement ◽  
In Vitro Antimicrobial Activity ◽  
Endodontic Sealers

scholarly journals In VitroEvaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials againstEnterococcus faecalis,Candida albicans, andStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Duddi Narendra Nirupama ◽  
Mohan Thomas Nainan ◽  
Rajendran Ramaswamy ◽  
Sethumadhavan Muralidharan ◽  
Hulimangala Hosakote Lingareddy Usha ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Direct Contact ◽  
Microbial Growth ◽  
Antimicrobial Property ◽  
Recurrent Infection ◽  
Antimicrobial Effect ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
Root Canal Sealers ◽  
Direct Contact Test

Root canal sealers that possess good antimicrobial property can prevent residual and recurrent infection and contribute to successful endodontic therapy. This study evaluated the antimicrobial activity of four endodontic sealers, AH Plus, Tubliseal EWT, EndoRez, and iRoot SP, against three different microorganisms,E. faecalis, C. albicans, andS. aureus, by direct contact test. 10 μL microbial suspensions were allowed to directly contact the four endodontic sealers for 1 hr at 37°C. Subsequently microbial growth was measured spectrophotometrically every 30 min for 18 hours. The microbial suspensions were simultaneously tested to determine the antimicrobial effect of components which are capable of diffusing into the medium. The results revealed that AH Plus and iRootSP had significantly higher antimicrobial activity againstE. faecalis. AH Plus and Tubliseal EWT showed significantly higher antimicrobial activity againstC. albicansandS. aureuscompared to iRoot SP and EndoRez. EndoRez showed the least antimicrobial activity against all the three microorganisms. Inhibition of microbial growth is related to the direct contact of microorganisms with the sealers. In conclusion AH Plus had significantly higher antimicrobial activity againstE. faecalis, C. albicans, andS. aureus.

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