scholarly journals Comparative Analysis of the Water Sorption and Cytotoxicity of Two different Denture Base Systems: An in vitro Study

2017 ◽  
Vol 18 (9) ◽  
pp. 771-774 ◽  
Author(s):  
Prashanth Shetty ◽  
Lokesh Chhapdia ◽  
Pranav Verma ◽  
Anshul Sahu ◽  
Narendra S Kushwaha ◽  
...  

ABSTRACT Aim Different biomaterials and techniques have been introduced in the field of prosthetic dentistry with the purpose of replacement and rehabilitation of the edentulous areas. Due to their shorter setting time, the light-activated restorative and prosthetic materials have the capability of releasing few amount of cytotoxic materials in the oral cavity. Polymer materials [urethane dimethacrylate (UDMA) and bis-acryl] are assumed to have high mechanical properties. Polymethyl methacrylate (PMMA) offers numerous advantages of being highly esthetic in nature and at the same time being cost-effective. Hence, this study aimed to assess and compare the water sorption and cytotoxicity of light-activated UDMA denture base resin and conventional heatactivated PMMA resin. Materials and methods This study included assessment and comparison of water sorption and cytotoxicity of heat-activated PMMA resin and light-activated UDMA denture base system. Fabrication of heat-activated PMMA resin and UDMA specimens was done by investing the wax patterns in stone molds using manufacturer's instructions. Contraction of the specimens was done for assessment of cytotoxicity and water resorption of the UDMA and PMMA resin samples. All the results were analyzed by Statistical Package for the Social Sciences software version 18.0. Chi-square test and one-way analysis of variance tests were used for the assessment of the level of significance; p < 0.05 was taken as significant. Results Mean lysis score observed in the PMMA and UDMA groups was 0.4 and 0.3 respectively. While observing at the 3 months time, the mean water resorption in the PMMA and UDMA groups was found to be 37.9 and 40.2 Significant difference in relation to water resorption was observed between the two study groups only at 3 months time. Conclusion Both materials used in this study are nontoxic. Furthermore, UDMA resin materials exhibited lower water resorption after more than 1 month of time of storage. Clinical significance Water resorption is similar for different denture base resin systems till 1 months time. How to cite this article Shetty P, Chhapdia L, Verma P, Sahu A, Kushwaha NS, Chaturvedi R, Manna S. Comparative Analysis of the Water Sorption and Cytotoxicity of Two different Denture Base Systems: An in vitro Study. J Contemp Dent Pract 2017;18(9):771-774.

2011 ◽  
Vol 22 (5) ◽  
pp. 388-391 ◽  
Author(s):  
Newton Sesma ◽  
Carlos Gil ◽  
William Antunes Kolikauskas ◽  
Rafael Andrade Silva ◽  
Claudio Mendes Pannuti

This in vitro study evaluated the temperature of dentures after different microwave irradiation protocols. Two complete dentures (one maxillary and one mandibular denture) were irradiated separately 4 times for each of the following 5 protocols: dentures immersed in water (G1- 6 min, G2- 3 min); dentures kept dry (G3- 6 min); dentures placed in the steam sterilizer (G4- 6 min, G5- 3 min). The final temperature of the dentures was gauged in a thin and in a thick area of each denture with an infrared thermometer. All groups presented an increase in the resin base temperature. The thin areas of the dentures underwent greater heating than the thick areas. There was no significant difference (p>0.05) between the final mean temperatures of dentures immersed in water for 6 (G1) and 3 min (G2). However, the final mean temperatures recorded in G1 and G2 exceeded 71°C and were significantly higher (<0.001) than the final mean temperatures recorded in the other groups. It may be concluded that denture base resins subjected to microwave irradiation immersed in water may be exposed to deleterious temperatures.


2017 ◽  
Vol 17 (4) ◽  
pp. 401 ◽  
Author(s):  
Rizwana Anjum ◽  
SunilV Dhaded ◽  
Shalini Joshi ◽  
ChandraShekhar Sajjan ◽  
Priyanka Konin ◽  
...  

2019 ◽  
Vol 801 ◽  
pp. 3-8 ◽  
Author(s):  
Sroisiri Thaweboon ◽  
Boonyanit Thaweboon ◽  
Futoshi Nakazawa

The adherence of microorganisms to denture base materials and the consequent formation of biofilms on these surfaces are contributing factors to biofilm-related oral and systemic diseases. Aspiration pneumonia is a potentially life-threatening respiratory infection associated with the entry of foreign materials into the bronchi. Vanillin-incorporated polymethyl methacrylate (PMMA) resin has been developed for the use in dentistry and demonstrated to have antimicrobial activity. Objective: To evaluate antimicrobial property of vanillin-incorporated PMMA denture base resin on biofilm formation of respiratory pathogens. Materials and methods: The heat polymerized PMMA denture base resin samples (Siam Cement Group, Thailand) were prepared according to the percentage of vanillin incorporation (0%, 0.1% and 0.5% vanillin). Another group of commercial resin samples without vanillin (Triplex®, Ivoclar Vivadent, USA) was prepared in the same manner. All samples were coated with sterile unstimulated saliva collected from three healthy adult volunteers at 37 °C for 60 min. The respiratory pathogenic bacteria used in this study were Staphylococcus aureus ATCC 5638, Streptococcus pneumoniae ATCC 49619, and Pseudomonas aeruginosa ATCC 27853. They were prepared to a concentration of approximately 107 colony forming unit (CFU)/mL. The bacterial biofilm formation was done in 96-well plate and incubated at 37°C for 24-48 h. The amount of biofilm was quantified by Cell Counting Kit WST-8 (Dojindo Molecular Technologies, USA) at 420 nm. All tests were performed in triplicate on three separate occasions. One-way ANOVA and Turkey’s test were used for the statistical analysis. Results: The vanillin-incorporated resin groups (0.1% and 0.5% vanillin) had a significant reduction of S. aureus and P. aeruginosa biofilm mass compared with resins without vanillin (0% vanillin and commercial resin groups). No significant difference was observed in the S. pneumonia biofilm formation. Up to 80% and 33% reductions of biofilm mass were demonstrated on P. aeruginosa and S. aureus, respectively. Conclusion: The incorporation of vanillin to denture base PMMA resin could significantly inhibit biofilm formation of respiratory pathogens. Using this PMMA resin, denture base materials with antimicrobial property can be applied to reduce a risk of respiratory infection in denture wearing patients.


2008 ◽  
Vol 62 (3) ◽  
pp. 217-222
Author(s):  
Milena Kostic ◽  
Stevo Najman ◽  
Jelena Kocic ◽  
Nebojsa Krunic ◽  
Zorica Ajdukovic ◽  
...  

Growth of HeLa cell culture in vitro was examined, in different concentrations of four resin materials extracts which are used for denture base making. Cell growth was evaluated through density, invert microscope counting, after 48 hours of incubation and through metabolic MTT test after 3 days. Extract was taken by incubation of material sample on 37 ?C in physiological solution, for 72 hours. It is given weaker growth, reduction of adherent cells count and phenotypic changes of cells grown in presence of extracts from all examined materials. Extracts of examined materials increase number of phyllopodic extensions on dose dependent manner.


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