scholarly journals Preparation, characterization and investigation of the anti-‎human ovarian cancer of silver nanoparticles green-formulated by Salvia officinalis leaf ‎aqueous extract ‎

2022 ◽  
Author(s):  
Danxia Luo ‎ ◽  
Arunachalam Chinnathambi ◽  
Tahani Awad Alahmadi ◽  
Prabakaran D.S. ◽  
Gaofeng Zhang
Keyword(s):  
Ovarian Cancer ◽  
Silver Nanoparticles ◽  
Cell Line ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Salvia Officinalis ◽  
Butylated Hydroxytoluene ◽  
Human Ovarian Cancer ◽  
Leaf Aqueous Extract ◽  
Ft Ir

IntroductionIn the present study, we decided to prepare and formulate a new chemotherapeutic drug (silver nanoparticles in ‎aqueous medium using Salvia officinalis leaf aqueous extract) for the treatment of human ovarian cancer in the in ‎vitro condition.Material and methodsThe organometallic chemistry tests such as Scanning Electron Microscopy (SEM), UV–Visible Spectroscopy ‎‎(UV-Vis), and Fourier Transformed Infrared Spectroscopy (FT‐IR) were used for characterizing of silver ‎nanoparticles. For investigating the antioxidant potentials of AgNO3, Salvia officinalis aqueous extract, and ‎silver nanoparticles, the DPPH test was used in the presence of butylated hydroxytoluene as the positive ‎control. To survey the cytotoxicity and anti-human ovarian cancer activities of AgNO3, Salvia officinalis ‎aqueous extract, and silver nanoparticles, MTT assay was used on the human ovarian cancer cell lines i.e., Caov-‎‎3‎, SK-OV-3, and PA-1‎. ‎ResultsIn UV-Vis, the clear peak in the wavelength of 421 nm indicated the formation of silver nanoparticles. In FT-IR ‎test, the presence of many antioxidant compounds with related bonds caused the excellent condition for ‎reducing of silver in the silver nanoparticles. The silver nanoparticles inhibited half of the DPPH molecules in ‎the concentration of 251 µg/mL. The best result of anti-human ovarian cancer effects of silver nanoparticles ‎against the above cell lines was observed in the case of the SK-OV-3 cell line. ‎ConclusionsSilver nanoparticles had very low cell viability and anti-human ovarian cancer properties dose-dependently ‎against Caov-3‎, SK-OV-3, and PA-1 cell lines without any cytotoxicity on the normal cell line (HUVECs). ‎

scholarly journals Anti-human ovarian cancer, cytotoxicity, and antioxidant effects of Nigella Sativa green-formulated Au nanoparticles: Describing a new chemotherapeutic supplement

2021 ◽  
Author(s):  
Haibo Ruan ◽  
Li Wang ◽  
Minyuan Wang ◽  
Weiwei Mo ◽  
Jichao Jin ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Ovarian Cancer ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Nigella Sativa ◽  
Antioxidant Properties ◽  
Butylated Hydroxytoluene ◽  
Human Ovarian Cancer ◽  
Chemotherapy Drugs

IntroductionThe recent research showed that the Gold nanoparticles (GNPs) formulated with Nigella Sativa aqueous extract having potent antioxidant and anti-human ovarian cancer activities in vitro condition.Material and methodsFor determinate the properties of the GNPs that were produced from the reaction between gold chloride solution with aqueous Nigella Sativa extract, we used UV–Visible Spectroscopy (UV-Vis), Field Emission Scanning Electron Microscopy (FE‐SEM), Fourier Transformed Infrared Spectroscopy (FT‐IR), and Transmission Electron Microscopy (TEM). For evaluating anti-ovarian cancer and cytotoxicity effects of GNPs, Au chloride, and Nigella Sativa aqueous extract, we used MTT assay.ResultsThe result of this test showed that GNPs have no cytotoxicity on normal cell line (HUVEC) and have potent anti-ovarian cancer features dose-dependently against PA-1, SK-OV-3, and SW-626 cell lines. The IC50 of GNPs were 249, 361, and 433 µg/mL against PA-1, SW-626, and SK-OV-3 cell lines, respectively. For evaluating the antioxidant features of GNPs, Au chloride, and Nigella Sativa aqueous extract, we used the DPPH test, in this test butylated hydroxytoluene was a positive control, the results of this test showed that the GNPs have an effective antioxidant feature. In the antioxidant test, the IC50 of GNPs and BHT were 144 and 201 µg/mL, respectively.ConclusionsProbably, potent anti-human ovarian cancer activities of GNPs formulated with Nigella Sativa aqueous seed extract because of antioxidant properties. After evaluating the effectiveness of this formulation in clinical trial researches, it can be a good alternative to chemotherapy drugs.

scholarly journals A comparative experiment on the anti-chronic myeloid leukemia capacities of AgNO3, ‎Scrophularia striata leaf aqueous extract, and silver nanoparticles containing natural ‎compounds ‎

2022 ◽  
Author(s):  
Yanmei Ma ◽  
Maryam Behtash ◽  
Hadis Yari ◽  
Mohammad Karimian ◽  
Naser Abbasi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Chronic Myeloid Leukemia ◽  
Cell Line ◽  
Cell Lines ◽  
Myeloid Leukemia ◽  
Blast Crisis ◽  
Butylated Hydroxytoluene ◽  
Ft Ir ◽  
Scrophularia Striata

IntroductionIn the current study, silver nanoparticles were prepared and synthesized in aqueous medium using Scrophularia ‎striata leaf extract as stabilizing and reducing agents. Also, we investigated the anti-chronic myeloid leukemia ‎potentials of silver nanoparticles against BV173 (chronic myeloid leukemia in blast crisis), CML-T1 (chronic ‎myeloid leukemia in lymphoid blast crisis), EM-2 (chronic myeloid leukemia in blast crisis; relapse after bone ‎marrow transplantation), and JOSK-M (chronic myeloid leukemia in myelomonocytic) cell lines. ‎Material and methodsSilver nanoparticles were characterized and analyzed using common nanotechnology techniques including UV-‎Vis.‎‏ ‏and FT-IR Spectroscopy, Field Emission-Scanning Electron Microscopy (FE-SEM), and Transmission ‎Electron Microscopy (TEM), and Energy Dispersive X‐ray Spectrometry (EDS). ‎ResultsFT-IR analysis offered antioxidant compounds in the nanoparticles were the sources of reducing power, ‎reducing silver ions to silver nanoparticles. FE-SEM and TEM images revealed a uniform spherical morphology ‎in size of 19.72 nm for the green synthesized nanoparticles. DPPH test revealed similar antioxidant potentials ‎for silver nanoparticles and butylated hydroxytoluene. Silver nanoparticles had very low cell viability and anti-‎chronic myeloid leukemia properties dose-dependently against JOSK-M, EM-2, CML-T1, and BV173 cell lines ‎without any cytotoxicity on the HUVEC cell line. The best result of cytotoxicity properties of silver ‎nanoparticles against the above cell lines was observed in the case of CML-T1 cell line. ‎ConclusionsAfter confirming in the in vivo and clinical trial studies, these nanoparticles can be administrated in humans for ‎the treatment of chronic myeloid leukemia.‎

scholarly journals Formulation of a novel anti-human bone tumor supplement by silver nanoparticles green-synthesized using Nigella sativa leaf aqueous extract

2021 ◽  
Author(s):  
Yijiang Huang ◽  
Ruimin Xu ◽  
Pei Fan ◽  
Liang Chen ◽  
Daosen Chen ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Cell Line ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Bone Tumor ◽  
Nigella Sativa ◽  
Human Bone ◽  
Butylated Hydroxytoluene ◽  
Dpph Test

IntroductionIn the present research, we formulated a modern chemotherapeutic drug by silver nanoparticles (AgNPs) containing Nigella sativa aqueous extract for the treatment of bone tumor.Material and methodsCharacterization of AgNPs was done by UV–Visible Spectroscopy (UV-Vis), Fourier Transformed Infrared Spectroscopy (FT‐IR), Transmission Electron Microscopy (TEM), and Field Emission Scanning Electron Microscopy (FE‐SEM). For investigating the antioxidant properties of AgNO3, N. sativa, and AgNPs, the DPPH test was used in the presence of butylated hydroxytoluene as the positive control. To survey the cytotoxicity and anti-bone tumor effects of AgNO3, N. sativa, and AgNPs, MTT assay was used on the human bone Ewing’s sarcoma (CADO-ES1 and MHH-ES1), human bone osteosarcoma (HOS and MG-63), and human bone chondrosarcoma (SW-1353 and CH-3573) cell lines.ResultsDPPH test revealed similar antioxidant potentials for N. sativa, AgNPs, and butylated hydroxytoluene. Silver nanoparticles had very low cell viability and anti-bone tumor properties dose-dependently against CADO-ES1, MHH-ES1, HOS, MG-63, SW-1353, and CH-3573 cell lines without any cytotoxicity on the normal cell line. The best result of anti-bone tumor properties of AgNPs against the above cell lines was seen in the case of the MG-63 cell line.ConclusionsAccording to the above findings, the silver nanoparticles containing N. sativa aqueous extract can be administrated in humans for the treatment of several types of bone tumors.

scholarly journals GREEN SYNTHESIS OF SILVER NANOPARTICLES USING GRAVIOLA LEAF AQUEOUS EXTRACT AT ROOM TEMPERATURE

2015 ◽  
Vol 2 (2) ◽  
pp. 6-10
Author(s):  
Ranjithkumar R ◽  
Chandar Shekar B ◽  
Senthil Kumaran C.K ◽  
Sharmila C ◽  
Simi V
Keyword(s):  
Silver Nanoparticles ◽  
Infrared Spectroscopy ◽  
Aqueous Extract ◽  
Sem Analysis ◽  
X Ray Diffraction ◽  
Electron Microscopic ◽  
Green Route ◽  
Leaf Aqueous Extract ◽  
Fourier Transformation Infrared Spectroscopy ◽  
Ft Ir

Improvement of green route for the synthesis of silver nanoparticles with plant extracts plays a very important role in nanotechnology without any harmful chemicals. The present investigation demonstrates the synthesis of silver nanoparticles by treating silver nitrate with Graviola leaf extract at room temperature.The effect of the extract on the formation of silver nanoparticles was characterized by UV-Vis spectrum, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and Scanning electron microscopic (SEM) analysis. The UV-Vis spectra results show a strong resonance centered on the surface of silvernanoparticles (AgNPs) at 400-450 nm. The Fourier transformation infrared spectroscopy spectral study demonstrates Graviola leaf aqueous extract acted as the reducing and stabilizing agent during the synthesis of silver nanoparticles. XRD and SEM studies revealed that the synthesized silver nanoparticles shows spherical in shape with average particles size around 30-70 nm.

scholarly journals Novel Curcumae Kwangsiensis mediated synthesis of silver nanoparticles for anti-lung cancer activity: a preclinical trial study

2021 ◽  
Author(s):  
Zhihong Liu ◽  
Zhuohong Zhang ◽  
Xiaomei Du ◽  
Ying Liu ◽  
Abdullah Alarfaj ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Silver Nanoparticles ◽  
Cell Lines ◽  
Cancer Cell ◽  
Aqueous Extract ◽  
Ag Nanoparticles ◽  
Human Lung ◽  
Human Lung Cancer ◽  
Lung Cancer Cell ◽  
Leaf Aqueous Extract

IntroductionThe present work indicated the green synthesis and characterization and cytotoxicity, antioxidant, and anti-human lung cancer activities of silver nanoparticles containing Curcumae Kwangsiensis Folium leaf aqueous extract.Material and methodsAg nanoparticles have been produced by mixing the AgNO3 solution with aqueous Curcumae Kwangsiensis Folium leaf extract. Characterization of Ag nanoparticles was done by FE‐SEM, FT‐IR, TEM, and UV-Vis. FE-SEM and TEM images revealed an average diameter of 15-21 nm for the nanoparticles. MTT assay was used on common human lung cancer cell lines i.e., lung well-differentiated bronchogenic adenocarcinoma (HLC-1), lung moderately differentiated adenocarcinoma (LC-2/ad), and lung poorly differentiated adenocarcinoma (PC-14) cell lines to survey the cytotoxicity and anti-human lung cancer effects of Ag nanoparticles.ResultsThey had very low cell viability and high anti-human lung cancer activities dose-dependently against HLC-1, LC-2/ad, and PC-14 cell lines without any cytotoxicity on the normal cell line (HUVEC). The IC50 of Ag nanoparticles were 249, 187, and 152 µg/mL against HLC-1, LC-2/ad, and PC-14 cell lines, respectively. The best results of cytotoxicity and anti-human lung cancer properties were seen in the concentration of 1000 µg/mL. Ag nanoparticles inhibited half of the DPPH molecules in the concentration of 135 µg/mL.ConclusionsMaybe significant anti-human lung cancer potentials of Ag nanoparticles synthesized by Curcumae Kwangsiensis Folium leaf aqueous extract against common human lung cancer cell lines are linked to their antioxidant activities. After confirming the above results in the clinical trial researches, this formulation can be administrated to treat human lung cancers in humans.

A New Clerodane Diterpene and Other Constituents from Ajuga chamaepitys ssp. laevigata

2004 ◽  
Vol 59 (5) ◽  
pp. 584-588 ◽  
Author(s):  
Gülaçtı Topçu ◽  
Gamze Kökdil ◽  
Zeynep Türkmen ◽  
Wolfgang Voelter ◽  
Eba Adou ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Ovarian Cancer Cell ◽  
Ovarian Cancer Cell Line ◽  
Cancer Cell Line ◽  
2D Nmr ◽  
Human Ovarian Cancer ◽  
Human Ovarian Cancer Cell ◽  
Spectroscopic Analyses

Abstract From Ajuga chamepitys ssp. laevigata, a new clerodane diterpene, ajugalaevigatic acid, has been isolated besides five known compounds, a diterpene, (13S)-15-hydroxylabd-8(17)-en-19-oic acid, a steroidal glucoside, 3-O-β -D-glucopyranosyl-stigmasta-5,25-diene, and triterpenes,α- and β -amyrin and ursolic acid. Their structural elucidation is based on NMR and MS spectroscopic analyses. For the new compound 2D NMR experiments were carried out. Ajugalaevigatic acid was tested against a panel of cytotoxic cell lines, and only found to be active against the A2780 human ovarian cancer cell line.

scholarly journals Gold nanoparticles formulated with Nigella aqueous extract having potent antioxidant ‎and ‎anti-human ovarian cancer activities in vitro condition

2021 ◽  
Author(s):  
Ju Li ◽  
Khodabakhsh Rashidi ◽  
Behnam Mahdavi ◽  
Samaneh Goorani ◽  
Mohammad Karimian ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gold Nanoparticles ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Treatment Strategies ◽  
Human Ovarian Cancer ◽  
Dna And Rna ◽  
Ovarian Cancer Cell Lines ◽  
Nigella Damascena

IntroductionRecently, various nanoparticles containing medicinal plants have been specifically designed to deliver anticancer drugs and nucleic acids such as DNA and RNA to cancer cells and as a result, they open up new avenues in cancer treatment strategies. In this study, gold nanoparticles were synthesized in aqueous medium using Nigella damascena extract as ‎stabilizing and reducing agents. ‎Material and methodsThe synthesized nanoparticles (AuNPs) were characterized using different techniques including UV-Vis. and ‎FT-IR spectroscopy, and Transmission electron microscopy (TEM). TEM images exhibited a uniform spherical ‎morphology in size of 21.64 nm for the biosynthesized nanoparticles. In the cellular and molecular part of the ‎recent study, the treated cells with AuNPs were assessed by MTT assay for 48h about the cytotoxicity and anti-‎human ovarian cancer ‎ properties on normal (HUVEC) and ovarian cancer cell lines i.e. PA-1, Caov-3, SW 626, ‎and SK-OV-3. ‎ResultsThe viability of malignant ovarian‎ cell line reduced dose-dependently in the presence of AuNPs. The IC50 of ‎AuNPs were 232‎, ‎204‎, ‎193‎, and ‎288 µg/mL against PA-1, Caov-3, SW 626, and SK-OV-3 cell lines, ‎respectively. In the antioxidant test, the IC50 of AuNPs and BHT against DPPH free radicals were 151 and 142 ‎‎µg/mL, respectively. ‎ConclusionsAfter clinical study, gold nanoparticles containing Nigella damascena leaf aqueous extract may be used to ‎formulate a new chemotherapeutic drug or supplement to treat the several types of human ovarian cancer.‎

scholarly journals Application of a novel lung protective drug formulated by silver nanoparticles containing Curcuma longa L leaf aqueous extract on α-Guttiferin-induced DNA fragmentation and apoptosis in lung HEL 299, MRC-5, IMR-90, CCD-19Lu, WI-38, and BEAS-2B cell lines

2020 ◽  
Author(s):  
Yi Han ◽  
Yuan Gao ◽  
Xiaoqing Cao ◽  
Akram Zangeneh ◽  
Mohammad Mahdi Zangeneh ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Dna Fragmentation ◽  
Cell Lines ◽  
Inflammatory Cytokines ◽  
Aqueous Extract ◽  
Mitochondrial Membrane ◽  
Caspase 3 ◽  
Curcuma Longa ◽  
Antioxidant Properties ◽  
Leaf Aqueous Extract

Abstract Recently, scientists have understood that metallic nanoparticles green-synthesized by medicinal plants have significant anti-cancer effects in the in vitro, in vivo, and clinical trial conditions. Also, the anti-lung cancer properties of metallic nanoparticles containing natural compounds have been indicated in many studies. In the recent research, we tried to investigate the application of a novel lung protective drug formulated by silver nanoparticles containing Curcuma longa L leaf aqueous extract on α-Guttiferin-induced DNA fragmentation and apoptosis in HEL 299, MRC-5, IMR-90, CCD-19Lu, WI-38, and BEAS-2B cell lines. Also, we assessed the concentrations of inflammatory cytokines, activity of caspase-3, and potential of mitochondrial membrane in the in vitro condition. Silver nanoparticles were characterized and analyzed by common physicochemical techniques including Transmission Electron Microscopy, Ultraviolet–Visible Spectroscopy, Fourier-Transform Infrared Spectroscopy, and Field Emission-Scanning Electron Microscopy. In the biological part of the present research, the cell viability of HEL 299, MRC-5, IMR-90, CCD-19Lu, WI-38, and BEAS-2B cell lines was measured by trypan blue assay. Caspase-3 activity was assessed by the caspase activity colorimetric assay kit and mitochondrial membrane potential was studied by Rhodamine123 fluorescence dye. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) test was used to show DNA fragmentation and apoptosis. Also, the Rat inflammatory cytokine assay kit was used to measure the concentrations of inflammatory cytokines. Silver nanoparticles-treated cell cutlers significantly (p ≤ 0.01) reduced the DNA fragmentation, caspase-3 activity, and inflammatory cytokines concentrations, and raised the mitochondrial membrane potential and cell viability in the high concentration of α-Guttiferin-treated HEL 299, MRC-5, IMR-90, CCD-19Lu, WI-38, and BEAS-2B cells. The best result of lung protective and antioxidant properties of silver nanoparticles containing Curcuma longa L leaf aqueous extract was seen in the high dose of silver nanoparticles i.e., 4 µg. Assessment of the antioxidant properties of silver nanoparticles was done with the common free radical scavenging test i.e., DPPH in the presence of butylated hydroxytoluene as the positive control. The nanoparticles inhibited half of the DPPH molecules in the concentration of 149 µg/mL. According to the above results, silver nanoparticles containing Curcuma longa L leaf aqueous extract can be administrated as a lung protective drug for the treatment of lung diseases after approving in the clinical trial studies in humans.

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