Synovial tissue morphology of the cricoarytenoid joint in the elderly: a histological comparison with the cricothyroid joint

Abstract Osteoarthritis is one of the most common chronic diseases in the elderly, which has caused a heavy burden to the family and society. At present, the clinical treatment can only be total joint replacement, but is hard to prevent the degeneration of cartilage tissue. The molecular mechanism of OA, especially the role of inflammation in disease progression, has been reported, but it is still unclear.Here, we first collected synovial tissues of 8 OA patients and 2 RA patients as controls, and detected the expression profiles of lncRNA, miRNA and mRNA in tissues by RNA-seq technology, and tried to find out the differentially expressed genes and key pathways in OA patients.Compared with the control group, 343 mRNAs, 270 lncRNAs and 247 miRNAs were significantly up-regulated, 232 mRNAs, 109 lncRNAs and 157 miRNAs were significantly down regulated. Then we predicted the targeted mRNAs of dysregulated lncRNAs. Through pathway enrichment and functional annotation of these target genes, we identified 12 differentially expressed transcripts related to inflammation, including CHST11, ALDH1A2, TREM1, IL-1β, IL-8, CCL5, LIF and miR-146a-5p, miR-335-5p, as well as lncRNA GAS5, LINC02288 and LOC101928134.In this study, we identified the differential expression of inflammation related genes and ncRNA in synovial tissue. These differentially expressed transcripts may indicate the network of ceRNA net in synovial tissue and its effect on OA progression. Our research will help to explore the pathogenesis and key therapeutic targets of OA.

Download Full-text

Mitochondria microcrystals deposition in some inherited diseases of lipid metabolism.

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100082145 ◽

1978 ◽

Vol 36 (2) ◽

pp. 256-257

Author(s):

S. Laoussadi ◽

A. Kahan ◽

G. Aubouy ◽

F. Delbarre

Keyword(s):

Synovial Tissue ◽

Storage Disease ◽

Metabolic Diseases ◽

Uranyl Acetate ◽

List Type ◽

Type Ii ◽

Type Number ◽

Lipid Storage Disease ◽

Thin Sections ◽

Mean Size

Several patients with Fabry's, Gaucher's diseases and hyperlipoproteinemia type II and with arthropatic manifestations were observed.As no histological explanation for these symptoms was available,an ultrastructural study of synovial tissue was done to establish an anatomoclinical relation.Material and Methods :synovial membrane samples were obtained by needle biopsies of the knee from three patients with arthropatic manifestations of each disease.They were fixed in 5% glutaraldehyde, postfixed in 1% osmium tetraoxyde and embedded in Epon 812. Thin sections coloured by uranyl acetate and lead citrate were observed with an Elmiskop I Siemens electron microscope.Two important phenomena were observed in synovial tissue:Specific patterns of each lipid storage disease,which are now well known.In all the three metabolic diseases, hydroxyapatite-like crystals were found. They are characterized by their intramitochondrial localization, without any relation with cristae,an anarchic disposition and a mean size of 550 A.Crystals may be found also free in the cytoplasm of synoviocytes Some micrographs suggest an evolution in four steps :a. mitochondria with only a few microcrystalsb. mitochondria stuffed with these structuresc. disruption of mitochondria membranesd. microcrystals appear free in the cytoplasm

Download Full-text

Cell surface changes during aging of human bladder epithelium examined by lectin probes and SEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162429 ◽

1990 ◽

Vol 48 (3) ◽

pp. 966-967

Author(s):

J. Jacob ◽

M.F.M. Ismail

Keyword(s):

The Elderly ◽

Ultrastructural Changes ◽

Pokeweed Mitogen ◽

Asymmetric Structure ◽

Luminal Surface ◽

Bladder Epithelium ◽

Aldehyde Groups ◽

Cell Surface Changes ◽

Phosphate Buffered Saline ◽

Surface Changes

Ultrastructural changes have been shown to occur in the urinary bladder epithelium (urothelium) during the life span of humans. With increasing age, the luminal surface becomes more flexible and develops simple microvilli-like processes. Furthermore, the specialised asymmetric structure of the luminal plasma membrane is relatively more prominent in the young than in the elderly. The nature of the changes at the luminal surface is now explored by lectin-mediated adsorption visualised by scanning electron microscopy (SEM).Samples of young adult (21-31 y old) and elderly (58-82 y old) urothelia were fixed in buffered 2% glutaraldehyde for 10 m and washed with phosphate buffered saline (PBS) containing Ca++ and Mg++ at room temperature. They were incubated overnight at 4°C in 0.1 M ammonium chloride in PBS to block any remaining aldehyde groups. The samples were then allowed to stand in PBS at 37°C for 2 h before incubation at 37°C for 30 m with lectins. The lectins used were concanavalin A (Con A), wheat germ agglutinin (WGA), phytohaemagglutinin (PHA) and pokeweed mitogen (PWM) at a concentration of 500 mg/ml in PBS at pH 7.A.

Download Full-text

The effect of dental geriatric curricula on attitudes toward the elderly

Journal of Dental Education ◽

10.1002/j.0022-0337.1988.52.9.tb02232.x ◽

1988 ◽

Vol 52 (9) ◽

pp. 516-518 ◽

Cited By ~ 2

Author(s):

J Mann ◽

TJ Bomberg ◽

JM Holtzman ◽

DB Berkey

Keyword(s):

The Elderly

Download Full-text

Speech-Language Pathologists' Attitudes Toward Aging and the Elderly

Contemporary Issues in Communication Science and Disorders ◽

10.1044/cicsd_30_f_146 ◽

2003 ◽

Vol 30 (Fall) ◽

pp. 146-155 ◽

Cited By ~ 5

Author(s):

Jeff Searl ◽

Rodney M. Gabel

Keyword(s):

The Elderly ◽

Speech Language Pathologists ◽

Attitudes Toward Aging

Download Full-text

Pharmacology Issues Related to Dementia Syndrome of Depression in Elderly Residents of Long-Term Care Settings

Perspectives on Neurophysiology and Neurogenic Speech and Language Disorders ◽

10.1044/nnsld25.2.61 ◽

2015 ◽

Vol 25 (2) ◽

pp. 61-69

Author(s):

Angel L. Ball ◽

Adina S. Gray

Keyword(s):

Depressive Symptoms ◽

The Elderly ◽

Environmental Communication ◽

Pharmacological Intervention ◽

Team Approach ◽

Discussion Paper ◽

Term Care ◽

Anticholinergic Medications ◽

Inappropriate Treatment ◽

Dementia Syndrome

Pharmacological intervention for depressive symptoms in institutionalized elderly is higher than the population average. Among the patients on such medications are those with a puzzling mix of symptoms, diagnosed as “dementia syndrome of depression,” formerly termed “pseudodementia”. Cognitive-communicative changes, potentially due to medications, complicate the diagnosis even further. This discussion paper reviews the history of the terminology of “pseudodementia,” and examines the pharmacology given as treatment for depressive symptoms in the elderly population that can affect cognition and communication. Clinicians can reduce the risk of misdiagnosis or inappropriate treatment by having an awareness of potential side effects, including decreased attention, memory, and reasoning capacities, particularly due to some anticholinergic medications. A team approach to care should include a cohesive effort directed at caution against over-medication, informed management of polypharmacology, enhancement of environmental/communication supports and quality of life, and recognizing the typical nature of some depressive signs in elderly institutionalized individuals.

Download Full-text