Mitochondria microcrystals deposition in some inherited diseases of lipid metabolism.

1978 ◽  
Vol 36 (2) ◽  
pp. 256-257
Author(s):  
S. Laoussadi ◽  
A. Kahan ◽  
G. Aubouy ◽  
F. Delbarre
Keyword(s):  
Synovial Tissue ◽  
Storage Disease ◽  
Metabolic Diseases ◽  
Uranyl Acetate ◽  
List Type ◽  
Type Ii ◽  
Type Number ◽  
Lipid Storage Disease ◽  
Thin Sections ◽  
Mean Size

Several patients with Fabry's, Gaucher's diseases and hyperlipoproteinemia type II and with arthropatic manifestations were observed.As no histological explanation for these symptoms was available,an ultrastructural study of synovial tissue was done to establish an anatomoclinical relation.Material and Methods :synovial membrane samples were obtained by needle biopsies of the knee from three patients with arthropatic manifestations of each disease.They were fixed in 5% glutaraldehyde, postfixed in 1% osmium tetraoxyde and embedded in Epon 812. Thin sections coloured by uranyl acetate and lead citrate were observed with an Elmiskop I Siemens electron microscope.Two important phenomena were observed in synovial tissue:Specific patterns of each lipid storage disease,which are now well known.In all the three metabolic diseases, hydroxyapatite-like crystals were found. They are characterized by their intramitochondrial localization, without any relation with cristae,an anarchic disposition and a mean size of 550 A.Crystals may be found also free in the cytoplasm of synoviocytes Some micrographs suggest an evolution in four steps :a. mitochondria with only a few microcrystalsb. mitochondria stuffed with these structuresc. disruption of mitochondria membranesd. microcrystals appear free in the cytoplasm

scholarly journals Acceleration, Containment and Emission of High Energy Flare Particles: Radio Evidence

1974 ◽  
Vol 57 ◽  
pp. 423-435 ◽  
Author(s):  
A. Boischot
Keyword(s):  
Shock Wave ◽  
Shock Waves ◽  
Solar Atmosphere ◽  
Impulsive Phase ◽  
High Energy ◽  
Continuous Phase ◽  
List Type ◽  
Radio Bursts ◽  
Type Ii ◽  
Type Number

The existence of non thermal radio bursts provide evidences for the acceleration of electrons in the solar atmosphere.It is shown, from the characteristics of the bursts, that the electrons are accelerated in at least four different phases: (1)An impulsive phase which gives μib and III bursts.(2)A gradual phase which gives μIV and S1IV bursts.(3)A quasi-continuous phase which gives S2IV bursts and noise storms.(4)An acceleration by shock waves gives type II bursts.(5)Eventually, another shock-wave acceleration giving the moIV burst.

scholarly journals Getting Epoxy Semi-Thin Sections to Stick to Glass Slides

2007 ◽  
Vol 15 (4) ◽  
pp. 52-52
Author(s):  
Gilbert (Gib) Ahlstrand
Keyword(s):  
Toluidine Blue ◽  
Room Temperature ◽  
Water Drop ◽  
List Type ◽  
Distilled Water ◽  
Type Number ◽  
Blow Down ◽  
Simple Method ◽  
Thin Sections ◽  
Glass Slides

Semi-thin sections don't always want to stick to glass slides, but subbing slides is usually not needed. For sections about 2 μm thick and no larger than 4 mm on a slide, this simple method works well for me:1)Clean 1x3 inch glass slides with an ethanol rinse, then air dry at room temperature or blow down with a hair dryer.2)Collect sections on a drop or two of distilled water on the slide, transferred there from the microtome with a clean fine tipped artists brush. Collect about 8-12 sections per drop.3)Warm the slide beneath the water drop from below using an alcohol lamp, fairly hot, but not to boil, of course. After drying by heating the sections stick quite well.4)Stain, usually with 0.2 μm filtered toluidine blue, again heating but gently this time, for about a minute, until stain “develops” the section.5)Rinse that stain off with distilled water from a squirt bottle, even directing the spray right onto the sections to get rid of any precipitate. Dry again gently with flame.The heating is the trick. There should not be any need for subbing or otherwise treating slides other than cleaning them.

A new method for searching intracellular stereo structures in plant tissues

1990 ◽  
Vol 48 (3) ◽  
pp. 720-721
Author(s):  
Yaqin Yao ◽  
Xuanli Jiang ◽  
Dongwei Hu
Keyword(s):  
Room Temperature ◽  
Plant Tissues ◽  
Specimen Preparation ◽  
List Type ◽  
Type Number ◽  
Cell Organelles ◽  
Mesophyll Cells ◽  
Thin Sections ◽  
Wheat Leaves ◽  
Ethanol Series

The procedure for specimen preparation consist of the following. 1.fixing the plant tissues routinely;2.washing the blocks thoroughly in PBS buffer;3.immersing the blocks in a PEG (polyethylene glycol 4000) series (50, 70, 90, 100%) and embedding in pure PEG at 50°C, and cooling slowly to room temperature;4.cutting into thin sections 5-10 nm thick by LKB-V microtome;5.washing the sections a few times in water, dehydrating in a graded ethanol series, and drying in a critical-point dryer using liquid CO2;6.sputter-coating with gold palladium; and7.investigating in a Hitachi S-450 scanning electron microscope.By this method, the distinct, uninjured intracellular stereo structures can be conveniently found without Joss of cell organelles. Moreover, there are advantages to studying their stereo relationships between various components of cells. Using wheat leaves, one can find chloroplasts, mitochondria, nuclei, nucleoli, and parts of the cytoskeleton system in the mesophyll cells and their sizes and distributions directly.

scholarly journals A young woman with multiple acyl-CoA dehydrogenase deficiency (MADD)

2021 ◽  
Vol 48 (s1) ◽  
pp. S4-S4
Author(s):  
HH Goebel ◽  
D Pehl ◽  
W Stenzel ◽  
U Schneider ◽  
M Schuelke
Keyword(s):  
Storage Disease ◽  
Childhood And Adolescence ◽  
Homozygous Mutation ◽  
List Type ◽  
Lipid Storage Disease ◽  
The Past ◽  
Minimal Contrast ◽  
Muscle Biopsies ◽  
Progressive Weakness ◽  
Myopathic Changes

A 31-year-old female hairdresser whose parents were first degree cousins complained of episodic attacks of headache, vomiting, and dizziness for the past eight years after an uneventful childhood and adolescence. Four years ago, she developed progressive weakness, muscle pain and difficulties walking and lifting her arms that she could not work in her profession anymore. She lost hair, weight and became amenorrhoic. Finally, her muscle weakness required intensive care. Early on her CK was mildly elevated to 237 U/l (normal < 167), but later to 900 and 1800. By MRI, skeletal muscles showed minimal contrast enhancement.The clinically suspected diagnosis of myositis prompted repeated muscle biopsies, which disclosed non-specific myopathic changes, scattered necrotic muscle fibers without inflammation, protein aggregation, or vacuolation by light microscopy, but abnormally structured mitochondria with inclusions by electron microscopy, and treatment with steroids without any clinical improvement.A panel of 1131 mitochondrial genes revealed a homozygous mutation in the ETFDH gene.LEARNING OBJECTIVESThis presentation will enable the learner to: 1.Discuss MADD as a mitochondrial and lipid storage disease2.Recognize the myopathology of MADD

Buffered Formalin for Primary Fixation and Preserving Tissue for a Long Time

1967 ◽  
Vol 25 ◽  
pp. 24-25
Author(s):  
I. Yamamoto ◽  
B. Rosario
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Phosphate Buffer ◽  
Standard Technique ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
List Type ◽  
Thin Sections ◽  
Long Time ◽  
Resin Mixture

It is known that suitably buffered formaldehyde can be used as a killing agent and primary fixative for electron microscopy(Pease).We were interested in how long buffered formalin preserves tissue. Thus, in this study, an attempt was made to observe ultrastructure of surgical material fixed and stored in buffered formalin for a long time.Pieces of fresh tissues were, fixed in buffered formalin at 4°C for one hour, and then placed at room temparature for weeks and months without changing solution.Buffered formalin used was prepared as follows:A. “ reagent grade ” formalinB. 0.05 M Sorensen's phosphate buffer, pH 7.2-7.4 mix 1 part of A and 9 parts of B to make 10 % solution.Pieces of tissues were then minced and postfixed in 1 % osmic acid buffered with veronal acetate for two hours or more. Postfixed tissues were then embedded in an epoxy resin mixture through standard technique. Thin sections were stained with uranyl acetate followed by lead, and examined in a Hitachi 11C electron microscope.

Solar Corona Investigation by the Coronal Line Photometer at Lomnický Peak

1994 ◽  
Vol 144 ◽  
pp. 431-434
Author(s):  
M. Minarovjech ◽  
M. Rybanský
Keyword(s):  
Solar Corona ◽  
Time Resolution ◽  
Active Regions ◽  
High Time ◽  
High Time Resolution ◽  
List Type ◽  
Type Number ◽  
Coronal Line ◽  
Global Cycle

AbstractThis paper deals with a possibility to use the ground-based method of observation in order to solve basic problems connected with the solar corona research. Namely:1.heating of the solar corona2.course of the global cycle in the corona3.rotation of the solar corona and development of active regions.There is stressed a possibility of high-time resolution of the coronal line photometer at Lomnický Peak coronal station, and use of the latter to obtain crucial observations.

The LDE-Type Flare Occurrence (1969-1993)

1994 ◽  
Vol 144 ◽  
pp. 279-282
Author(s):  
A. Antalová
Keyword(s):  
Time Series ◽  
Fourier Analysis ◽  
Sunspot Cycle ◽  
List Type ◽  
Type Number ◽  
Solar Cycles ◽  
Type Iv ◽  
Long Duration ◽  
Cycle Maximum ◽  
Flare Index

AbstractThe occurrence of LDE-type flares in the last three cycles has been investigated. The Fourier analysis spectrum was calculated for the time series of the LDE-type flare occurrence during the 20-th, the 21-st and the rising part of the 22-nd cycle. LDE-type flares (Long Duration Events in SXR) are associated with the interplanetary protons (SEP and STIP as well), energized coronal archs and radio type IV emission. Generally, in all the cycles considered, LDE-type flares mainly originated during a 6-year interval of the respective cycle (2 years before and 4 years after the sunspot cycle maximum). The following significant periodicities were found:• in the 20-th cycle: 1.4, 2.1, 2.9, 4.0, 10.7 and 54.2 of month,• in the 21-st cycle: 1.2, 1.6, 2.8, 4.9, 7.8 and 44.5 of month,• in the 22-nd cycle, till March 1992: 1.4, 1.8, 2.4, 7.2, 8.7, 11.8 and 29.1 of month,• in all interval (1969-1992):a)the longer periodicities: 232.1, 121.1 (the dominant at 10.1 of year), 80.7, 61.9 and 25.6 of month,b)the shorter periodicities: 4.7, 5.0, 6.8, 7.9, 9.1, 15.8 and 20.4 of month.Fourier analysis of the LDE-type flare index (FI) yields significant peaks at 2.3 - 2.9 months and 4.2 - 4.9 months. These short periodicities correspond remarkably in the all three last solar cycles. The larger periodicities are different in respective cycles.

Discussion following the presentation by F. Deubner

1977 ◽  
Vol 36 ◽  
pp. 69-74
Keyword(s):  
List Type ◽  
The Sun ◽  
Type Number ◽  
Harmonic Motion ◽  
Locally Excited ◽  
Long Period ◽  
Coherent Wave ◽  
Short Period ◽  
Global Modes

The discussion was separated into 3 different topics according to the separation made by the reviewer between the different periods of waves observed in the sun :1) global modes (long period oscillations) with predominantly radial harmonic motion.2) modes with large coherent - wave systems but not necessarily global excitation (300 s oscillation).3) locally excited - short period waves.

Prominences and Solar Activity

1979 ◽  
Vol 44 ◽  
pp. 357-372
Author(s):  
Z. Švestka
Keyword(s):  
Solar Activity ◽  
Solar Cycle ◽  
List Type ◽  
Type Number ◽  
Flare Loops ◽  
Filament Activation

The following subjects were discussed:(1)Filament activation(2)Post-flare loops.(3)Surges and sprays.(4)Coronal transients.(5)Disk vs. limb observations.(6)Solar cycle variations of prominence occurrence.(7)Active prominences patrol service.Of all these items, (1) and (2) were discussed in most detail and we also pay most attention to them in this report. Items (3) and (4) did not bring anything new when compared with the earlier invited presentations given by RUST and ZIRIN and therefore, we omit them.

A New System For Optomanual Semiautomatic Quantitative Image Analysis

1977 ◽  
Vol 35 ◽  
pp. 210-211
Author(s):  
H.P. Rohr
Keyword(s):  
Image Analysis ◽  
Volume Density ◽  
List Type ◽  
Distribution Analysis ◽  
Type Number ◽  
Point Counting ◽  
Human Eye ◽  
Quantitative Image ◽  
Point Density ◽  
Electronic Counting

Today, in image analysis the broadest possible rationalization and economization have become desirable. Basically, there are two approaches for image analysis: The image analysis through the so-called scanning methods which are usually performed without the human eye and the systems of optical semiautomatic analysis completely relying on the human eye.The new MOP AM 01 opto-manual system (fig.) represents one of the very promising approaches in this field. The instrument consists of an electronic counting and storing unit, which incorporates a microprocessor and a keyboard for choice of measuring parameters, well designed for easy use.Using the MOP AM 01 there are three possibilities of image analysis:the manual point counting,the opto-manual point counting andthe measurement of absolute areas and/or length (size distribution analysis included).To determine a point density for the calculation of the corresponding volume density the intercepts lying within the structure are scanned with the light pen.

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