scholarly journals Evaluation of chemical profile and antioxidant potential of Trichodesma indicum (L.) R.Br.

2017 ◽  
Vol 9 (3) ◽  
pp. 416
Author(s):  
Mahendra S Khyade ◽  
Subhash N Varpe ◽  
Anup D Padwal
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Pharmaceutical Preparations ◽  
Reducing Power ◽  
Total Phenolic ◽  
Good Source ◽  
Maximum Inhibition ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

<p>The present study was conducted to evaluate the antioxidant activity and chemical composition of aqueous methanolic extract of <em>Trichodesma indicum </em>(L.) R.Br. The extract was tested for antioxidant activity using various <em>in vitro</em> models viz., DPPH, ABTS, NO, FRAP, total antioxidant activity and reducing power. The total phenolic and flavonoid contents were found to be equivalents to 97.83 ± 0.08 µg of gallic acid and 45.45 ± 0.19 µg of rutin /mg of dried aqueous methanolic extract, respectively. Among various antioxidant assays performed, maximum inhibition was observed in the ABTS (IC<sub>50</sub> 17.13 ±  0.23 μg) followed by DPPH (IC<sub>50 </sub>80.64  ±  0.29 μg) and NO (IC<sub>50 </sub>4.12 ± 0.02  μg) assay. GC-MS analysis revealed the presence of over 38 compounds, the prevailing compounds were hexadecanoic acid, β-sitosterol, ethyl iso-allocholate and 9,12,15,-Octadecatrienoicacid.  The HPLC analysis further confirmed the presence of rutin, salicylic acid and benzoic acid. This confirms that <em>T. indicum</em> could be the good source of natural antioxidant for industrial and pharmaceutical preparations.</p>

scholarly journals Evaluation of Free Radical Scavenging and Polyphenolic Contents of Bark of Pterygota alata Roxb

2014 ◽  
Vol 6 (3) ◽  
pp. 543-552 ◽  
Author(s):  
N. Jahan ◽  
M. S. Parvin ◽  
A. Khan ◽  
N. Das ◽  
M. S. Islam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Soluble Fraction ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Deciduous Tree ◽  
Total Phenolic ◽  
Phenolic And Flavonoid

Pterygota alata is a large deciduous tree of Malvaceae family. The present study was designed to evaluate in vitro antioxidant activity of stem bark of the plant. Ferric reducing power (FRP) test, 1, 1- diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging test and total antioxidant capacity (TAC) assay were used to detect the antioxidant activity. Total phenolic and flavonoid contents were also determined as they are well known phytochemicals with antioxidant property. The bark of the plant showed efficient reducing power as well as free radical scavenging property (IC50 values 52.25 - 172.05). The bark also found rich in total phenolic and flavonoid content. The highest amount of total phenolic content was found in chloroform soluble fraction (29.898 µg/mL) followed by ethyl acetate soluble fraction (15.88 µg/mL). The highest content of total flavonoid also detected in chloroform soluble fraction (107.56 µg/mL) but followed by crude ethanol extract (98.66 µg/mL). Overall, the bark of the plant possesses significant antioxidant activity, therefore can be used as a good natural source of antioxidant. © 2014 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: http://dx.doi.org/10.3329/jsr.v6i3.17586 J. Sci. Res. 6 (3), 543-552 (2014)

scholarly journals Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of FolkloricLannea coromandelicaUsingIn VitroandIn VivoAssays

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Tekeshwar Kumar ◽  
Vishal Jain
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Intragastric Administration ◽  
Total Antioxidant ◽  
Liver And Kidney ◽  
Mda Content ◽  
Phenolic And Flavonoid

The aim of this study was to determine the impending antioxidant properties of different extracts of crude methanolic extract (CME) of leaves ofLannea coromandelica(L. coromandelica) and its two ethyl acetate (EAF) and aqueous (AqF) subfractions by employing various establishedin vitrosystems and estimation of total phenolic and flavonoid content. The results showed that extract and fractions possessed strong antioxidant activityin vitroand among them, EAF had the strongest antioxidant activity. EAF was confirmed for its highest phenolic content, total flavonoid contents, and total antioxidant capacity. The EAF was found to show remarkable scavenging activity on 2,2-diphenylpicrylhydrazyl (DPPH) (EC5063.9 ± 0.64 µg/mL), superoxide radical (EC508.2 ± 0.12 mg/mL), and Fe2+chelating activity (EC506.2 ± 0.09 mg/mL). Based on ourin vitroresults, EAF was investigated forin vivoantioxidant assay. Intragastric administration of the EAF can significantly increase levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels, and decrease malondialdehyde (MDA) content in the liver and kidney of CCl4-intoxicated rats. These new evidences show thatL. coromandelicabared antioxidant activity.

scholarly journals Studies on In Vitro Antioxidant Activity of Methanolic Extract and Fractions of Ficus Hispida Lin. Fruits

2016 ◽  
Vol 8 (3) ◽  
pp. 371-380 ◽  
Author(s):  
M. S. Hossain ◽  
S. Parvin ◽  
S. Dutta ◽  
M. S. I. Mahbub ◽  
M. E. Islam
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Traditional Use ◽  
Total Antioxidant ◽  
Ficus Hispida

The present study was designed to confirm the traditional use of the fruits of Ficus hispida Linn. (Moraceae) as an antioxidant agent. Fruits of the plant extracted with methanol and crude methanol extract (CME) were further fractionated with n-hexane, chloroform, and ethyl acetate. All the fractions, n-hexane (NHF), chloroform (CHF), ethyl acetate (EAF), aqueous (AQF) and CME were preliminary screened for in vitro antioxidant activity and total phenolic and total flavonoid content. In DPPH radical scavenging assay, CME exhibited highest scavenging activity (IC50 = 11.20 µg/mL) as compared to other fractions. In this assay, IC50 of reference standard BHT was 5.10 µg/mL. The reducing power of the samples was in the order as AQF > CME > CHF > EAF > NHF. The results for hydrogen peroxide scavenging activity indicated that CME, EAF and AQF had almost the same scavenging activity except NHF. Total antioxidant capacity of CME and other fractions were ranked as CHF > AQF > CME > EAF > NHF.  In the assay of antioxidant constituents (total phenol and total flavonoids content), the CME had highest phenolic and flavonoids content. The results indicate that Ficus hispida fruits could be considered as a potential source of natural antioxidant.

scholarly journals Antioxidant activity of Trachystemon orientalis (L.) G. Don (Borage) grown and eaten as food in Ordu, Turkey

2015 ◽  
Vol 61 (4) ◽  
pp. 40-51 ◽  
Author(s):  
Melek Col Ayvaz
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Total Antioxidant Capacity ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Synthetic Antioxidants ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

SummaryIntroductıon: Instead of synthetic antioxidants, using of natural products with antioxidant activity is demanded.Objectıve: The aim of this study is to evaluate the antioxidant activity and total phenolic and flavonoid contents of theTrachystemon orientalis(borage) obtained from Ordu.Methods: The water and ethanol extracts of the borage plant were investigated by using severalin vitroantioxidant activity tests such as total antioxidant capacity, O2•−,•OH, DPPH and ABTS radicals scavenging activities, hydrogen peroxide scavenging activity, FRAP, Fe2+chelating and reducing power assays in order to make effective comparisons.Results: Water extract of theT. orientalishad the more phenolic (90 mg GA/g extract) and flavonoid (56.88 mg CT/g extract) contents than ethanol extract. Furthermore, both of the extracts showed strong antioxidant and radical scavenging activities determined by differentin vitromodels. The highest total antioxidant capacity as ascorbic acid equivalents of 244.50 mg/g extract was also calculated for water extract. Generally, a relationship between total phenolic content and antioxidant activity was established. It is also an important finding for an edible food source that the phenolic and flavonoid content and antioxidative activities for water extract was higher than the ethanol extract.Conclusıon:T. orientaliscould be used as a food ingredient instead of synthetic antioxidants and all results will contribute to the recent increase in investigations on using natural products in many areas such as food, pharmacy, alternative medicine and natural therapy.

scholarly journals Evaluation of cholinesterase inhibitory and antioxidant potential of Wedelia chinensis: possible implications in alleviating Alzheimer’s disease

2021 ◽  
Author(s):  
Md. Aminul Islam ◽  
Shahed Zaman ◽  
Kushal Biswas ◽  
Md. Yusuf Al-Amin ◽  
Md. Kamrul Hasan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Active Compound ◽  
Inhibitory Activity ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Brain Lipid ◽  
Total Antioxidant

Abstract Background Wedelia chinensis has been reported as a folk medicine for the treatment of different diseases including neurodegenerative disease. Although the plant has been studied well for diverse biological activities, the effect of this plant in neurological disorder is largely unknown. The present study was undertaken to evaluate the cholinesterase inhibitory and antioxidant potential of W. chinensis. Methods The extract and fractions of the plant were evaluated for acetylcholinesterase and butyrylcholinesterase inhibitory activity by modified Ellman method. The antioxidant activity was assessed in several in vitro models/assays such as reducing power, total antioxidant capacity, total phenolic and flavonoid content, scavenging of 2,2’-diphenyl-1-picrylhydrazyl (DPPH) free radical and hydroxyl radical, and inhibition of brain lipid peroxidation. Chromatographic and spectroscopic methods were used to isolate and identify the active compound from the extract. Results Among the extract and functions, aqueous fraction (AQF) and ethylacetate fraction (EAF) exhibited high inhibition against acetylcholinesterase (IC50: 40.02 ± 0.164 µg/ml and 57.76 ± 0.370 µg/ml) and butyrylcholinesterase (IC50: 31.79 ± 0.182 µg/ml and 48.41 ± 0.053 µg/ml). Similarly, the EAF and AQF had high content of phenolics and flavonoids and possess strong antioxidant activity in several antioxidant assays including DPPH and hydroxyl radical scavenging, reducing power and total antioxidant activity. They effectively inhibited the peroxidation of brain lipid in vitro with IC50 values of 45.20 ± 0.995 µg/ml and 25.53 ± 0.042 µg/ml, respectively. A significant correlation was observed between total flavonoids and antioxidant and cholinesterase inhibitory activity. Activity guided chromatographic separation led to the isolation of a major active compound from the EAF and its structure was elucidated as apigenin by spectral analysis. Conclusions The potential ability of W. chinensis to inhibit the cholinesterase activity and peroxidation of lipids suggest that the plant might be useful for the management of AD.

scholarly journals Comparative Studies of Phytochemicals Analysis and in vitro Antioxidant Properties of Various Solvent Extracts of Sphaeranthus indicus Collected from three Different Climatic Zones of Central India

2021 ◽  
Vol 33 (9) ◽  
pp. 2237-2243
Author(s):  
Pooja N. Akhand ◽  
Veena Sharma ◽  
Anupam K. Pathak
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Central India ◽  
Total Phenolic ◽  
Climatic Zones ◽  
Phenolic Contents ◽  
Total Antioxidant ◽  
Sphaeranthus Indicus

In present study, the total phenolic, flavonoid, tannin contents and the antioxidant activity of various solvent extracts of Sphaeranthus indicus collected from three different regions of central India were assessed. Plants extracts were prepared using Soxhlation method, while the total phenols, flavonoids and tannins were measured by the spectrophotometric method. DPPH, metal chelating, nitric oxide, superoxide oxide scavenging activity and FRAP, reducing power, total antioxidant assays were also evaluated. The highest phenolic contents 268.22 GAE equivalent, flavonoids 441.33 QE equivalents and tannin content was 120.32 tannic acid equivalents (mg/g) obtained from ethanolic extracts of sample SIEE-1 compared to other two plants extract. Similarly, the highest flavonoid contents was observed in SIAE-1 aqueous extract and lowest in SIAE-2 and SIAE-3. Extract of SIEE-1 possessed maximum antioxidant potentiality and SIAE-2 shown the least antioxidant activity in all assays. It could be concluded that different agroclimatic conditions have effects on the total phenolics, flavonoids, tannin contents and antioxidant potentiality of S. indicus plant.

scholarly journals Evaluation of Antioxidant Activity and Polyphenols Content of the Hydro-methanolic Extract from Saccocalyx satureioides Coss and Dur

2020 ◽  
Vol 10 (4) ◽  
pp. 188-190
Author(s):  
Soulaf MEHLOUS ◽  
Fatima BENCHIKH ◽  
Hassiba BENABDALLAH ◽  
Karima LOUCIF ◽  
Chahrazed KAOUDOUNE ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
In Vitro Models ◽  
Total Phenolic ◽  
Dry Extract ◽  
Good Resource ◽  
Gallic Acid Equivalent ◽  
Phenolic And Flavonoid

This study aimed to estimate the total phenolic and flavonoid contents and to evaluate the antioxidant activity of the Hydro-methanolic from Saccocalyx satureioides Coss and Dur aerial part. Total polyphenols contents were determined using Folin-Ciocalteu’s reagent. The flavonoids were estimated using the method of Aluminum chloride (AlCl3). The antioxidant capacity was evaluated using two in vitro models (the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and reducing power). Total phenolic and flavonoid content in the extract were 171.34 ±1.43 mg Gallic acid equivalent/g of dry extract (GAE/g) and 18.6 ± 0.46 mg Quercetin equivalent / g of dry extract (QE/g), respectively. The methanol extract has an important capacity to scavenge the free radical DPPH with an IC50 of 0.03 ± 0. 0024 mg/ml. In addition, the plant extract exhibited high reducing power with an IC50 of 0.54 ± 0.00625 mg/ml. The results of the present study may prove that the medicinal plants are a good resource of natural antioxidants. Keywords: Antioxidant activity, Saccocalyx satureioides, polyphenols, flavonoids.

scholarly journals Antioxidant Activity of Milling Fractions of Selected Cereals

2012 ◽  
Vol 11 (1) ◽  
pp. 45-56 ◽  
Author(s):  
Eva Ivanišová ◽  
Miroslav Ondrejovič ◽  
Stanislav Šilhár
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Frap Assay ◽  
Total Antioxidant ◽  
Total Antioxidant Potential ◽  
Phenolic And Flavonoid

Antioxidant Activity of Milling Fractions of Selected CerealsThe aim of this study was to evaluate antioxidant potential of four milling fractions of selected cereals grew in the year 2009 and 2010. Free radical scavenging activity of samples was measured using DPPH assay and reducing power was determined using FRAP assay. Secondary, total phenolic and flavonoid content of cereal extracts was evaluated. We found that flour fractions (break flour and reduction flour) showed the lower proportion of the total antioxidant potential than bran fractions (fine bran and coarse bran), which was balanced in observed years. Extract from barley had the highest values of antioxidant activity and polyphenol content.

scholarly journals In vitro antioxidant, anti-inflammatory and thrombolytic properties of leaf extract and fractions of Pterocarpus mildbraedii harms

2021 ◽  
Vol 22 (3) ◽  
pp. 65-74
Author(s):  
O.A. Fajobi ◽  
B.O. Emma-Okon ◽  
O.O. Oyedapo
Keyword(s):  
Methanolic Extract ◽  
Reducing Power ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Beneficial Effects ◽  
Total Antioxidant ◽  
Anti Inflammatory ◽  
Ig G ◽  
Gallic Acid Equivalent

The study was undertaken to evaluate the in vitro antioxidant, anti-inflammatory and thrombolytic properties of methanolic extract (PMME) and fractions of the leaf of Pterocarpus mildbraedii with a view to exploring the therapeutic potentials of the plant especially as far as treatment and management of oxidative, inflammatory and heart related disorders are concerned. The study involved collection, identification, extraction with 80%methanol, fractionation of hydromethanolic extract of P. mildbraedii leaves with n-hexane, ethyl acetate and nbutanol; evaluation of the contents for total phenolics, flavonoids as well as vitamins C and E. Evaluation of the antioxidant and anti-inflammatory potentials of the plant using 2, 2'-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing power, hydrogen peroxide reduction, total antioxidant capacity (TAC), and stabilization of erythrocyte membrane and inhibition of albumin denaturation were also carried out. The total phenolic content ranged between 13.31 ± 0.12 and 234.99 ± 2.64 GAE (gallic acid equivalent)/g, flavonoid content ranged between 44.30 ± 0.74 and 228.74 ± 7.44 QE (quercetin equivalent)/g, vitamin C content was 11.98 ± 0.04 mg/g while vitamin E content was 8.62 ± 0.29 ìg/g. The fractions exhibited potent and significant antioxidant and antiinflammatory activities which are concentration-dependent, as well as appreciable thrombolytic activities. Moreover, the extract and fractions compared favourably with various reference drugs used in the study. It was observed that the extract and its fractions possessed bioactive components with beneficial effects in the management of oxidative, inflammatory and heart-related conditions. Keywords: Pterocarpus mildbraedii methanolic extract, Antioxidant, Anti-inflammatory, Thrombolytic, Membrane stability, Haemoglobin, Denaturation.

scholarly journals Evaluation of cholinesterase inhibitory and antioxidant activity of Wedelia chinensis and isolation of apigenin as an active compound

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Md. Aminul Islam ◽  
Shahed Zaman ◽  
Kushal Biswas ◽  
Md. Yusuf Al-Amin ◽  
Md. Kamrul Hasan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Active Compound ◽  
Inhibitory Activity ◽  
Reducing Power ◽  
Total Phenolic ◽  
Brain Lipid ◽  
Aqueous Fraction ◽  
Total Antioxidant

Abstract Background Wedelia chinensis has been reported as a folk medicine for the treatment of different diseases including neurodegenerative disease. Although the plant has been studied well for diverse biological activities, the effect of this plant in neurological disorder is largely unknown. The present study was undertaken to evaluate the cholinesterase inhibitory and antioxidant potential of W. chinensis. Methods The extract and fractions of the plant were evaluated for acetylcholinesterase and butyrylcholinesterase inhibitory activity by modified Ellman method. The antioxidant activity was assessed in several in vitro models/assays such as reducing power, total antioxidant capacity, total phenolic and flavonoid content, scavenging of 2,2′-diphenyl-1-picrylhydrazyl (DPPH) free radical and hydroxyl radical, and inhibition of brain lipid peroxidation. Chromatographic and spectroscopic methods were used to isolate and identify the active compound from the extract. Results Among the fractions, aqueous fraction (AQF) and ethylacetate fraction (EAF) exhibited high inhibition against acetylcholinesterase (IC50: 40.02 ± 0.16 μg/ml and 57.76 ± 0.37 μg/ml) and butyrylcholinesterase (IC50: 31.79 ± 0.18 μg/ml and 48.41 ± 0.05 μg/ml). Similarly, the EAF and AQF had high content of phenolics and flavonoids and possess strong antioxidant activity in several antioxidant assays including DPPH and hydroxyl radical scavenging, reducing power and total antioxidant activity. They effectively inhibited the peroxidation of brain lipid in vitro with IC50 values of 45.20 ± 0.10 μg/ml and 25.53 ± 0.04 μg/ml, respectively. A significant correlation was observed between total flavonoids and antioxidant and cholinesterase inhibitory activity. Activity guided chromatographic separation led to the isolation of a major active compound from the EAF and its structure was elucidated as apigenin by spectral analysis. Conclusions The potential ability of W. chinensis to inhibit the cholinesterase activity and peroxidation of lipids suggest that the plant might be useful for the management of AD.

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