scholarly journals Evaluation of cholinesterase inhibitory and antioxidant potential of Wedelia chinensis: possible implications in alleviating Alzheimer’s disease

2021 ◽  
Author(s):  
Md. Aminul Islam ◽  
Shahed Zaman ◽  
Kushal Biswas ◽  
Md. Yusuf Al-Amin ◽  
Md. Kamrul Hasan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Active Compound ◽  
Inhibitory Activity ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Brain Lipid ◽  
Total Antioxidant

Abstract Background Wedelia chinensis has been reported as a folk medicine for the treatment of different diseases including neurodegenerative disease. Although the plant has been studied well for diverse biological activities, the effect of this plant in neurological disorder is largely unknown. The present study was undertaken to evaluate the cholinesterase inhibitory and antioxidant potential of W. chinensis. Methods The extract and fractions of the plant were evaluated for acetylcholinesterase and butyrylcholinesterase inhibitory activity by modified Ellman method. The antioxidant activity was assessed in several in vitro models/assays such as reducing power, total antioxidant capacity, total phenolic and flavonoid content, scavenging of 2,2’-diphenyl-1-picrylhydrazyl (DPPH) free radical and hydroxyl radical, and inhibition of brain lipid peroxidation. Chromatographic and spectroscopic methods were used to isolate and identify the active compound from the extract. Results Among the extract and functions, aqueous fraction (AQF) and ethylacetate fraction (EAF) exhibited high inhibition against acetylcholinesterase (IC50: 40.02 ± 0.164 µg/ml and 57.76 ± 0.370 µg/ml) and butyrylcholinesterase (IC50: 31.79 ± 0.182 µg/ml and 48.41 ± 0.053 µg/ml). Similarly, the EAF and AQF had high content of phenolics and flavonoids and possess strong antioxidant activity in several antioxidant assays including DPPH and hydroxyl radical scavenging, reducing power and total antioxidant activity. They effectively inhibited the peroxidation of brain lipid in vitro with IC50 values of 45.20 ± 0.995 µg/ml and 25.53 ± 0.042 µg/ml, respectively. A significant correlation was observed between total flavonoids and antioxidant and cholinesterase inhibitory activity. Activity guided chromatographic separation led to the isolation of a major active compound from the EAF and its structure was elucidated as apigenin by spectral analysis. Conclusions The potential ability of W. chinensis to inhibit the cholinesterase activity and peroxidation of lipids suggest that the plant might be useful for the management of AD.

scholarly journals Evaluation of cholinesterase inhibitory and antioxidant activity of Wedelia chinensis and isolation of apigenin as an active compound

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Md. Aminul Islam ◽  
Shahed Zaman ◽  
Kushal Biswas ◽  
Md. Yusuf Al-Amin ◽  
Md. Kamrul Hasan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Active Compound ◽  
Inhibitory Activity ◽  
Reducing Power ◽  
Total Phenolic ◽  
Brain Lipid ◽  
Aqueous Fraction ◽  
Total Antioxidant

Abstract Background Wedelia chinensis has been reported as a folk medicine for the treatment of different diseases including neurodegenerative disease. Although the plant has been studied well for diverse biological activities, the effect of this plant in neurological disorder is largely unknown. The present study was undertaken to evaluate the cholinesterase inhibitory and antioxidant potential of W. chinensis. Methods The extract and fractions of the plant were evaluated for acetylcholinesterase and butyrylcholinesterase inhibitory activity by modified Ellman method. The antioxidant activity was assessed in several in vitro models/assays such as reducing power, total antioxidant capacity, total phenolic and flavonoid content, scavenging of 2,2′-diphenyl-1-picrylhydrazyl (DPPH) free radical and hydroxyl radical, and inhibition of brain lipid peroxidation. Chromatographic and spectroscopic methods were used to isolate and identify the active compound from the extract. Results Among the fractions, aqueous fraction (AQF) and ethylacetate fraction (EAF) exhibited high inhibition against acetylcholinesterase (IC50: 40.02 ± 0.16 μg/ml and 57.76 ± 0.37 μg/ml) and butyrylcholinesterase (IC50: 31.79 ± 0.18 μg/ml and 48.41 ± 0.05 μg/ml). Similarly, the EAF and AQF had high content of phenolics and flavonoids and possess strong antioxidant activity in several antioxidant assays including DPPH and hydroxyl radical scavenging, reducing power and total antioxidant activity. They effectively inhibited the peroxidation of brain lipid in vitro with IC50 values of 45.20 ± 0.10 μg/ml and 25.53 ± 0.04 μg/ml, respectively. A significant correlation was observed between total flavonoids and antioxidant and cholinesterase inhibitory activity. Activity guided chromatographic separation led to the isolation of a major active compound from the EAF and its structure was elucidated as apigenin by spectral analysis. Conclusions The potential ability of W. chinensis to inhibit the cholinesterase activity and peroxidation of lipids suggest that the plant might be useful for the management of AD.

scholarly journals Studies on In Vitro Antioxidant Activity of Methanolic Extract and Fractions of Ficus Hispida Lin. Fruits

2016 ◽  
Vol 8 (3) ◽  
pp. 371-380 ◽  
Author(s):  
M. S. Hossain ◽  
S. Parvin ◽  
S. Dutta ◽  
M. S. I. Mahbub ◽  
M. E. Islam
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Traditional Use ◽  
Total Antioxidant ◽  
Ficus Hispida

The present study was designed to confirm the traditional use of the fruits of Ficus hispida Linn. (Moraceae) as an antioxidant agent. Fruits of the plant extracted with methanol and crude methanol extract (CME) were further fractionated with n-hexane, chloroform, and ethyl acetate. All the fractions, n-hexane (NHF), chloroform (CHF), ethyl acetate (EAF), aqueous (AQF) and CME were preliminary screened for in vitro antioxidant activity and total phenolic and total flavonoid content. In DPPH radical scavenging assay, CME exhibited highest scavenging activity (IC50 = 11.20 µg/mL) as compared to other fractions. In this assay, IC50 of reference standard BHT was 5.10 µg/mL. The reducing power of the samples was in the order as AQF > CME > CHF > EAF > NHF. The results for hydrogen peroxide scavenging activity indicated that CME, EAF and AQF had almost the same scavenging activity except NHF. Total antioxidant capacity of CME and other fractions were ranked as CHF > AQF > CME > EAF > NHF.  In the assay of antioxidant constituents (total phenol and total flavonoids content), the CME had highest phenolic and flavonoids content. The results indicate that Ficus hispida fruits could be considered as a potential source of natural antioxidant.

scholarly journals Increased Phenolic Content and Enhanced Antioxidant Activity in Fermented Glutinous Rice Supplemented with Fu Brick Tea

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 671 ◽  
Author(s):  
Xiao Xu ◽  
Wenxiu Hu ◽  
Siduo Zhou ◽  
Chuanhai Tu ◽  
Xiudong Xia ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Phenolic Content ◽  
Reducing Power ◽  
Total Phenolic ◽  
Food Ingredient ◽  
Antioxidant Power ◽  
Functional Activities ◽  
Glutinous Rice

Glutinous rice-based foods have a long history are consumed worldwide. They are also in great demand for the pursuit of novel sensory and natural health benefits. In this study, we developed a novel fermented glutinous rice product with the supplementation of Fu brick tea. Using in vitro antioxidant evaluation and phenolic compounds analysis, fermentation with Fu brick tea increased the total phenolic content and enhanced the antioxidant activity of glutinous rice, including scavenging of 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical, 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical, and hydroxyl radical, ferric-reducing antioxidant power, and ferric ion reducing power and iron chelating capability. Besides, compared with traditional fermented glutinous rice, this novel functional food exhibited a stronger activity for protecting DNA against hydroxyl radical-induced oxidation damage. Quantitative analysis by HPLC identified 14 compounds covering catechins and phenolic acids, which were considered to be positively related to the enhanced antioxidant capability. Furthermore, we found that 80% ethanol was a suitable extract solvent compared with water, because of its higher extraction efficiency and stronger functional activities. Our results suggested that this novel fermented glutinous rice could serve as a nutraceutical food/ingredient with special sensory and functional activities.

scholarly journals Comparative Studies of Phytochemicals Analysis and in vitro Antioxidant Properties of Various Solvent Extracts of Sphaeranthus indicus Collected from three Different Climatic Zones of Central India

2021 ◽  
Vol 33 (9) ◽  
pp. 2237-2243
Author(s):  
Pooja N. Akhand ◽  
Veena Sharma ◽  
Anupam K. Pathak
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Central India ◽  
Total Phenolic ◽  
Climatic Zones ◽  
Phenolic Contents ◽  
Total Antioxidant ◽  
Sphaeranthus Indicus

In present study, the total phenolic, flavonoid, tannin contents and the antioxidant activity of various solvent extracts of Sphaeranthus indicus collected from three different regions of central India were assessed. Plants extracts were prepared using Soxhlation method, while the total phenols, flavonoids and tannins were measured by the spectrophotometric method. DPPH, metal chelating, nitric oxide, superoxide oxide scavenging activity and FRAP, reducing power, total antioxidant assays were also evaluated. The highest phenolic contents 268.22 GAE equivalent, flavonoids 441.33 QE equivalents and tannin content was 120.32 tannic acid equivalents (mg/g) obtained from ethanolic extracts of sample SIEE-1 compared to other two plants extract. Similarly, the highest flavonoid contents was observed in SIAE-1 aqueous extract and lowest in SIAE-2 and SIAE-3. Extract of SIEE-1 possessed maximum antioxidant potentiality and SIAE-2 shown the least antioxidant activity in all assays. It could be concluded that different agroclimatic conditions have effects on the total phenolics, flavonoids, tannin contents and antioxidant potentiality of S. indicus plant.

scholarly journals Estimation of flavonoids and screening of in vitro antioxidant activity of various extracts of aerial parts of Blepharis maderaspatensis by hydroxyl radical and total antioxidant activity

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1204-1209
Author(s):  
Jambula Dinesh Babu ◽  
Bhuvaneswari Santharam ◽  
Kottai Muthu Arumugam
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Bioactive Compound ◽  
Antioxidant Potential ◽  
Total Flavonoids ◽  
Total Antioxidant Activity ◽  
Aerial Parts ◽  
Total Antioxidant ◽  
Total Flavonoids Content

Antioxidants are substances that hamper or inhibit the oxidation of oxidizable substrates in the cells. The aerial parts of Blepharis maderaspatensis dry powder was extracted with various solvents (PE, EA and methanol) through Soxhlet extractor. The aerial parts of different concentrates (Pet.ether, ethyl acetate and methanol) of Blepharis maderaspatensis was evaluated for its in-vitro antioxidant potential by hydroxyl radical, total antioxidant activity taking ascorbate used as standard for the both methods and total flavonoids content was estimated as equivalent to rutin. The methanolic concentrates of Blepharis maderaspatensis & standard exhibited antioxidant potential possessing IC50 210µg/ml & 62µg/ml (hydroxyl radical) 200µg/ml & 57µg/ml (Total antioxidant activity) respectively. An IC50 value was originate that methanolic concentrates of Blepharis maderaspatensis more efficient in hydroxyl radical, total antioxidant activity compared EA & PE concentrates. The methanolic and EA concentrates of Blepharis maderaspatensis showed the total flavonoids content (11.230±0.22 and 2.858±0.56 mg of rutin equivalent/g of powder) respectively. The difference in scavenging potential of the extracts can be due to variation in the percentage of bioactive compound flavonoids present in methanolic extracts. Invitro antioxidant studies obviously show methanolic concentrates of Blepharis maderaspatensis have better antioxidant activity due to the presence of total flavonoids content. The findings from the study reveal that the antioxidative potentials of methanolic concentrates Blepharis maderaspatensis could serve as free radical inhibitors, possibly acting as natural antioxidants.

scholarly journals Antioxidant Activity of Milling Fractions of Selected Cereals

2012 ◽  
Vol 11 (1) ◽  
pp. 45-56 ◽  
Author(s):  
Eva Ivanišová ◽  
Miroslav Ondrejovič ◽  
Stanislav Šilhár
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Frap Assay ◽  
Total Antioxidant ◽  
Total Antioxidant Potential ◽  
Phenolic And Flavonoid

Antioxidant Activity of Milling Fractions of Selected CerealsThe aim of this study was to evaluate antioxidant potential of four milling fractions of selected cereals grew in the year 2009 and 2010. Free radical scavenging activity of samples was measured using DPPH assay and reducing power was determined using FRAP assay. Secondary, total phenolic and flavonoid content of cereal extracts was evaluated. We found that flour fractions (break flour and reduction flour) showed the lower proportion of the total antioxidant potential than bran fractions (fine bran and coarse bran), which was balanced in observed years. Extract from barley had the highest values of antioxidant activity and polyphenol content.

scholarly journals Evaluation of chemical profile and antioxidant potential of Trichodesma indicum (L.) R.Br.

2017 ◽  
Vol 9 (3) ◽  
pp. 416
Author(s):  
Mahendra S Khyade ◽  
Subhash N Varpe ◽  
Anup D Padwal
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Pharmaceutical Preparations ◽  
Reducing Power ◽  
Total Phenolic ◽  
Good Source ◽  
Maximum Inhibition ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

<p>The present study was conducted to evaluate the antioxidant activity and chemical composition of aqueous methanolic extract of <em>Trichodesma indicum </em>(L.) R.Br. The extract was tested for antioxidant activity using various <em>in vitro</em> models viz., DPPH, ABTS, NO, FRAP, total antioxidant activity and reducing power. The total phenolic and flavonoid contents were found to be equivalents to 97.83 ± 0.08 µg of gallic acid and 45.45 ± 0.19 µg of rutin /mg of dried aqueous methanolic extract, respectively. Among various antioxidant assays performed, maximum inhibition was observed in the ABTS (IC<sub>50</sub> 17.13 ±  0.23 μg) followed by DPPH (IC<sub>50 </sub>80.64  ±  0.29 μg) and NO (IC<sub>50 </sub>4.12 ± 0.02  μg) assay. GC-MS analysis revealed the presence of over 38 compounds, the prevailing compounds were hexadecanoic acid, β-sitosterol, ethyl iso-allocholate and 9,12,15,-Octadecatrienoicacid.  The HPLC analysis further confirmed the presence of rutin, salicylic acid and benzoic acid. This confirms that <em>T. indicum</em> could be the good source of natural antioxidant for industrial and pharmaceutical preparations.</p>

scholarly journals In Vitro Antioxidant Activity, Total Phenolic, Flavonoid and Tannin Contents in the Ajuga Bracteosa Wall. Ex Benth, Grown at Middle Hill Climatic Condition of Western Himalayas

2020 ◽  
Vol 5 (3) ◽  
pp. 198-203
Author(s):  
Anchala Guglani ◽  
H. K. Pandey ◽  
Rajeshwar K.K. Arya ◽  
Madhu Bala
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Alcoholic Extract ◽  
Western Himalayas ◽  
Plant Parts ◽  
Phytochemical Contents

The antioxidant activity of aqueous and alcoholic extracts of different plant parts viz, leaves, flower, stem and root of Ajuga bracteosa was investigated against various in-vitro antioxidant assays. The total phenolic, flavonoid and tannin contents also estimated. The results revealed the significant antioxidant potential and variation in the IC50, EC50 and phytochemical contents among all the plant parts. The aqueous extract of leaves exhibited significantly (P<0.05) highest antioxidant activity on 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acixd), 2,2-diphenyl-1-picryl hydrazyl, and potassium ferricyanide reducing power assay, with IC50 values of 0.2707±0.0008, 0.4409±0.0020, and EC50 value 0.3413±0.0030 mg/mL, respectively, followed by the other parts of the plant. The leaves extract also possess the highest total phenolics, flavonoid and tannin contents among all the parts. Similarly, the aqueous extract is better than the alcoholic extract of different parts as far as phytochemical contents and antioxidant activity concerned. The present study revealed that the aqueous extract of leaves had the highest antioxidant potential, which correlated with the high level of total phenolic fl flavonoid, and tannin contents. Therefore, higher the phytochemical contents, higher will be the antioxidant potential.

scholarly journals IN VITRO - ANTIOXIDANT AND PRELIMINARY PHYTOCHEMICAL ANALYSIS OF AEGLE MARMELOS (L.) CORREA. LEAF EXTRACT

2017 ◽  
Vol 10 (6) ◽  
pp. 367
Author(s):  
Samidha M Pawaskar ◽  
Sasangan Kc
Keyword(s):  
Hydroxyl Radical ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Aegle Marmelos ◽  
Leaf Powder ◽  
Phenolic And Flavonoid

Objective: In this study, the leaf powder of Aegle marmelos (L.) Correa. was subjected to preliminary phytochemical and in vitro antioxidant analysis. Methods: The freshly prepared plant leaf extract was subjected to preliminary phytochemical screening, which revealed the presence of alkaloids, tannins, saponins, flavonoids, glycosides, phenolic compounds, terpenoids, and steroids. Reducing power, superoxide (SO) anion radical, nitric oxide (NO) radical, and hydroxyl radical scavenging assays were carried out to evaluate the antioxidant potential of the methanolic leaf extract of this plant. The amounts of total phenolic and flavonoid compounds were also determined. Results: This study has revealed that the A. marmelos (L.) Correa. leaf extract showed considerably high amounts of most of the phytochemicals, total antioxidant capacity, total phenolic, and flavonoid content. The study also indicated that the A. marmelos (L.) Correa. showed comparatively good scavenging activity, i.e., inhibition of hydroxyl radical, NO and SO anion scavenging and reducing power activities when compared with the respective standards. Conclusion: The leaf powder of A. marmelos (L.) Correa. can be used as easily accessible source of natural antioxidant and as a possible food supplement or in pharmaceutical industry.

scholarly journals Evaluation of phytochemical, antioxidant and cytotoxic potential of Sesbania grandiflora Linn.

2018 ◽  
Vol 7 (2) ◽  
pp. 191-198
Author(s):  
Shruti Gupta ◽  
◽  
Kishori G Apte ◽  
◽  
Keyword(s):  
Brine Shrimp ◽  
Reducing Power ◽  
In Vitro Cytotoxicity ◽  
Total Phenolic ◽  
Asian Countries ◽  
Leaf Extracts ◽  
Sesbania Grandiflora ◽  
Total Antioxidant ◽  
Potent Drug

Sesbania grandiflora Linn. is perennial branching, small erect quick-growing short-lived soft-wooded tree, mostly cultivated in southeast Asian countries including India and grows primarily in hot, humid environments. This study investigated on phytochemical values- total phenolic flavonoid, tannins and saponin contents for Sesbania grandiflora leaf extracts. The total antioxidant capacity, DPPH, reducing power, superoxide scavenging and Iron chelating effects were examined by established methods. In-vitro cytotoxicity by brine shrimp bioassay. Both the extracts of Sesbania grandiflora were found to possess moderate to high amounts of phytochemical contents. The total phenolic, flavonoid and saponin contents were found significantly higher in EQSG whereas tannins were more in AQSG. The EQSG and AQSG possessed low IC50 for H2O2 Scavenging (32.9, 27.3µg/ml), TAC (35.6, 41.8µg/ml) and moderate for DPPH (114.9, 113µg/ml) and reducing power (176.3, 181.6µg/ml) respectively. The Brine shrimp lethality bioassay showed a significantly high LC50 value with EQSG (10313µg/ml) and AQSG (12773µg/ml) compared to cyclophosphamide (110µg/ml) in cytotoxic assay. The results evidenced the potential of Sesbania grandiflora Linn. as potent drug with antioxidant and cytotoxic activity and could be useful for preparation of nutraceuticals for pharmaceutical use in the treatment of various human diseases and its complications

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