Biodiversity of Parasitoids Present in Agroforestry in Brazil

The aim of this study is to report the parasitoids collected on many substrates in the agroforestry area in Brazil. The experimental study was carried out in an agroforestry area in the south of Goiás, Brazil. Traps made of metal containers. They served as baits to attract flies: fish, bovine kidneys, human feces and chicken viscera deposited inside metal containers, on a layer of sand. This sand was sieved after the pupae were extracted and subsequently placed individually in small glass containers to obtain flies and parasitoids. From 745 pupae of dipteran collected 684 parasitoids emerged from 111 pupae. The most frequent species in this study was Aphaereta sp. (Hymenoptera: Braconidae: Alysiinae) 52.6%. The total percentage of parasitism obtained at work was 14.9%. Brachymeria podagrica Fabricius (Hymenoptera: Chalcididae) was the species that presented the highest percentage of parasitism with 55.0%, parasitizing Ophyra aenescens L. (Diptera: Muscidae) on human feces substrate

A Mixture of Chicken Viscera, Housefly Larvae and Spirulina Waste as Replacement of Fishmeal in Nile Tilapia (Oreochromis niloticus) Diets

This research investigated different blends of spirulina waste (SW), chicken viscera meal (CVM) and housefly maggot meal (HMM) as alternative protein sources on growth performance, feed utilization and body composition of Oreochromis niloticus fingerlings. Triplicate groups of male fish (initial mean weight, 6.09±0.5g) were fed a commercial feed Skretting (SK), fish meal (FM) based-diet D0 (30%FM), diet D1 (FM+SW+HMM), diet D2 (FM+SW+CVM), diet D3 (FM+HMM+CVM) and diet D4 (SW+HMM+CVM) for 84 days. Diets were isonitrogenous (35% crude protein) and isoenergetic (19 KJ/g gross energy). No effects were found on survival rate and body protein content of fish fed experimental diets. Specific growth rate, weight gain, feed conversion ratio and protein efficiency ratio (PER) of fish fed D0 and SK did not differ significantly from those fed diet D3. These parameters were significantly lower in fish fed the other diets (P<0.05). Feed intake significantly decreased in fish fed diet D1 and D2 while PER obtained did not differ than those fed control diets. The higher profit index was recorded in the tested diets and the lower in the control diets. The results of this study indicate that fishmeal level for O. niloticus could be reduced to 5 % by inclusion of CVM and HMM in combination.

Effect of Cation on Efficiency of Aspegillus flavus Bioflocculant Produced from Chicken Viscera Hydrolysate

<p class="0abstract"><strong>Abstract—</strong> The cations are needed as stimulants for effective flocculation by the cation-dependent bioflocculants. Addition of metal ions (cations) can counterbalance anionic functional groups of both bioflocculant and solid particles thereby increasing the bioflocculant adsorption to suspended particles. In the present study, addition of all dose of both Ca²⁺ and Mg²⁺ stimulated the efficiency of the bioflocculant with optimum flocculation efficiency of 95% recorded with 5 mL of 1% Ca²⁺_.While lower dose (1 – 2 mL) of Al³⁺ also stimulated the bioflocculant to about 94%, Na⁺ and Fe³⁺ inhibited flocculation at all doses tested. K⁺ slightlyenhanced the flocculation at 4 - 10mL of 1%. The present cationic bioflocculant can be suggested as a substitute for chemical flocculants.</p>

A Comparison between Chicken Viscera and Housefly Maggot Cultured from this By-products for Nile Tilapia Diets: Growth Performance, Feed Utilization and Whole Body Composition

Aims: The feeding trials were conducted to compare the effects of partial fishmeal replacement by two different animal protein sources on growth performance, feed utilization efficiency and body composition of juvenile Nile tilapia. Place and Duration of Study: The study was carried out at the Aquaculture Research Center, Department of Zoology, Faculty of Sciences and Technics, University of Abomey-Calavi for a period of 84 days. Methodology: Three isonitrogenous and isoenergetic diets were formulated containing fishmeal (CD), chicken viscera meal (CVM) and housefly maggot meal (HMM), as partial fishmeal replacement. Commercial diet Skretting (SK) was used as reference diet. All male Oreochromis niloticus with an average initial body weight of 8.65 g were fed thrice a day to apparent satiation. Each treatment was randomly attributed to three replicates. Results: Fish fed all experimental diets showed no effects (P>.05) on survival rate (91.33-96.00 %), condition factor (1.85-1.9), protein efficient ratio (2.05-2.37) and feed conversion ratio (1.21-1.40), although higher values were observed with control diets. Final mean weight and daily weight gain of fish fed HMM diet (88.31 g ; 0.95 g. days-1) were not significantly different from those fed control diets C (88.54 g; 0.95 g/j) and Sk (87.59 g; 0.94 g/j) respectively. Growth performances significantly decreased (P<.05) in CVM group (75.09 g; 0.75 ± g/j). Whole-body protein contents were similar in all groups, whereas lipid content was highest in those fed CVM. Conclusion: The results indicated that 200 g fishmeal per kilo diet can be successfully replaced with 250 g.Kg-1 of HMM without adverse effect on growth and feed utilization whereas CVM inclusion did not perform also well.

Necrophagous Fly Assembly: Evaluation of Species Bait Preference in Field Experiments

The assembly of species that colonize animal organic matter, their relative abundance, and dynamics are affected by the environmental and biogeographical conditions to which these resources are exposed. Baited trap studies are essential for research on the diversity, seasonality, distribution and population dynamics of necrophagous flies. Decomposing baits provide the necessary stimulus for flies to aggregate on them. In this study, three types of bait of animal origin with different organic chemical composition were compared in terms of the diversity, richness, abundance, and species composition of saprophagous flies species that were attracted to them. Bone-meal (BM), cow liver (CL), and rotten chicken viscera (CV) were used as bait to collect flies. In total, 3,387 Calliphoridae, Sarcophagidae, and Muscidae adult flies were collected. The most abundant species were Lucilia ochricornis (Wiedemann 1830), Chrysomya albiceps (Wiedemann 1819) (Diptera: Calliphoridae), Peckia (Sarcodexia) lambens (Wiedemann 1830) (Diptera: Sarcophagidae), and Ophyra capensis (Wiedemann 1818) (Diptera: Muscidae). The type of bait had significant effects on both the total richness (F2,18 = 57.08; P &lt; 0.0001) and the effective number of species (F2,18 = 12.81; P = 0.0003) per trap. The average richness was higher in traps baited with chicken viscera, followed by cow liver and finally by bone-meal. The composition of cow liver and bone meal species constitute subsets of the species collected with chicken viscera, thus using the three baits would not increase the number of species detected. These results indicate that chicken viscera is the most efficient bait for testing or assessing necrophagous fly diversity.

Enzymatic hydrolysis of chicken viscera proteins in the presence of an ionic liquid as a strategy to improve the antioxidant properties of the hydrolysates

Aiming to explore the use of ionic liquids (ILs) not yet described in the literature, this work evaluated the hydrolysis of proteins from chicken viscera using the protease Alcalase modified and unmodified by the IL tetramethylammonium bromide. The protein hydrolysates produced in the presence of the IL presented values of antioxidant activities 40% higher than the hydrolysates obtained without IL. In addition, with the presence of the IL, it was possible to obtain protein hydrolysates from chicken viscera with similar antioxidant activities, compared to the protein hydrolysates produced without IL, using 1/3 of the amount of enzyme.