Mesenchymal Stem Cells (Mscs) in Scanning Electron Microscopy (SEM) World

Niche Journal ◽  
2014 ◽  
Vol 2 (2) ◽  
pp. 22-24 ◽  
Author(s):  
Asuman Ozen ◽  
Irem Gul Sancak ◽  
Meral Tiryaki ◽  
Ahmet Ceylan ◽  
Ferda Alparslan Pinarli ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Stem Cells ◽  
Scanning Electron Microscopy ◽  
Mesenchymal Stem Cells ◽  
Scanning Electron

Bioengineered three-dimensional physical constructs from quince seed mucilage for human adipose-derived mesenchymal stem cells

2020 ◽  
Vol 35 (3) ◽  
pp. 240-253
Author(s):  
Ekin Şimşek ◽  
Burak Karaca ◽  
Yavuz Emre Arslan
Keyword(s):  
Electron Microscopy ◽  
Stem Cells ◽  
Scanning Electron Microscopy ◽  
Mesenchymal Stem Cells ◽  
Three Dimensional ◽  
Mechanical Analysis ◽  
Extraction Yield ◽  
Freeze Dried ◽  
Seed Mucilage ◽  
Scanning Electron

In this study, we aimed at fabricating a novel porous physical construct from quince seed mucilage for translational medicine applications. To achieve this goal, quince seed mucilage was extracted, molded, and freeze-dried. After being freeze-dried, the molded constructs were chemically crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide to maintain the mechanical integrity of the structure. The fabricated scaffolds were characterized in-depth by scanning electron microscopy, Fourier transform infrared spectroscopy, Brunauer–Emmett–Teller analysis, thermogravimetric analysis, and dynamic mechanical analysis in addition to the swelling, liquid uptake, and porosity tests. The extraction yield of mucilage was calculated to be 6.28% ± 0.40% (n = 3). The swelling ratio of crosslinked quince seed mucilage–derived scaffolds was found to be 12,677.50% ± 388.82% (n = 3), whereas the porosity of crosslinked quince seed mucilage–derived scaffolds was 83.43% ± 2.84% (n = 3). The analyses confirmed the crosslinked quince seed mucilage–derived scaffolds to be possessed interconnected, highly porous structure. Afterward, human adipose-derived mesenchymal stem cells were seeded on the crosslinked quince seed mucilage–derived scaffolds, and the cell viability on the scaffolds was assessed with 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The MTT results revealed the scaffolds not to be possessed any cytotoxic effect on seeded cells. Human adipose-derived mesenchymal stem cells adhesion and migration on the crosslinked quince seed mucilage–derived scaffolds were also evaluated histologically using hematoxylin and eosin staining in addition to scanning electron microscopy analysis. In conclusion, we believe that crosslinked quince seed mucilage–derived scaffolds have the potential to be an alternative to routinely used polysaccharides in regenerative medicine applications.

scholarly journals Scanning Electron Microscopy: Bridging the Gap from Stem Cells to Hydrogels

2010 ◽  
Vol 16 (S2) ◽  
pp. 596-597 ◽  
Author(s):  
L-M Joubert
Keyword(s):  
Electron Microscopy ◽  
Stem Cells ◽  
Scanning Electron Microscopy ◽  
Scanning Electron

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.

scholarly journals Cockle Shell-Derived Aragonite CaCO3 Nanoparticles for Co-Delivery of Doxorubicin and Thymoquinone Eliminates Cancer Stem Cells

2020 ◽  
Vol 21 (5) ◽  
pp. 1900 ◽  
Author(s):  
Kehinde Muibat Ibiyeye ◽  
Abu Bakar Zakaria Zuki
Keyword(s):  
Electron Microscopy ◽  
Stem Cells ◽  
Scanning Electron Microscopy ◽  
Calcium Carbonate ◽  
Cancer Stem Cells ◽  
Cellular Migration ◽  
Aldh Activity ◽  
Single Drug ◽  
Cockle Shell ◽  
Scanning Electron

Cancer stem cells CSCs (tumour-initiating cells) are responsible for cancer metastasis and recurrence associated with resistance to conventional chemotherapy. This study generated MBA MD231 3D cancer stem cells enriched spheroids in serum-free conditions and evaluated the influence of combined doxorubicin/thymoquinone-loaded cockle-shell-derived aragonite calcium carbonate nanoparticles. Single loaded drugs and free drugs were also evaluated. WST assay, sphere forming assay, ALDH activity analysis, Surface marker of CD44 and CD24 expression, apoptosis with Annexin V-PI kit, cell cycle analysis, morphological changes using a phase contrast light microscope, scanning electron microscopy, invasion assay and migration assay were carried out; The combination therapy showed enhanced apoptosis, reduction in ALDH activity and expression of CD44 and CD24 surface maker, reduction in cellular migration and invasion, inhibition of 3D sphere formation when compared to the free drugs and the single drug-loaded nanoparticle. Scanning electron microscopy showed poor spheroid formation, cell membrane blebbing, presence of cell shrinkage, distortion in the spheroid architecture; and the results from this study showed that combined drug-loaded cockle-shell-derived aragonite calcium carbonate nanoparticles can efficiently destroy the breast CSCs compared to single drug-loaded nanoparticle and a simple mixture of doxorubicin and thymoquinone.

scholarly journals Osteogenic Differentiation Potential of Adipose-Derived Mesenchymal Stem Cells Cultured on Magnesium Oxide/Polycaprolactone Nanofibrous Scaffolds for Improving Bone Tissue Reconstruction

2020 ◽  
Author(s):  
Zahra Niknam ◽  
Ali Golchin ◽  
Mostafa Rezaei –Tavirani ◽  
Parviz Ranjbarvan ◽  
Hakimeh Zali ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Magnesium Oxide ◽  
Composite Nanofibers ◽  
Nanofibrous Scaffolds ◽  
Scanning Electron ◽  
Cells Cultured

Purpose: Recently, bone tissue engineering as a new strategy is used to repair and replace bone defects due to limitations in allograft and autograft methods. In this regard, we prepared nanofibrous scaffolds composed of polycaprolactone and magnesium oxide nanoparticles using the electrospinning technique for possible bone tissue engineering applications. Methods: The fabricated composites were characterized via scanning electron microscopy imaging of scaffolds and seeded cells, water contact angle, DAPI staining, and MTT assay. Then osteogenic differentiation of adipose-derived mesenchymal stem cells cultured on this composite scaffold was determined by standard osteogenic marker tests, including alkaline phosphatase activity, calcium deposition, and expression of osteogenic differentiation genes in the laboratory conditions. Results: The Scanning electron microscopy analysis demonstrated that the diameter of nanofibers significantly decreased from 1029.25±209.349 µm to 537.83+0.140 nm, with the increase of MgO concentration to 2% (p<0.05). Initial adhesion and proliferation of the adipose-derived mesenchymal stem cells on magnesium oxide/polycaprolactone scaffolds were significantly enhanced with the increasing of magnesium oxide concentration (p<0.05). The 2% magnesium oxide/polycaprolactone nanofibrous scaffold showed significant increase in ALP activity (p<0.05) and osteogenic-related gene expressions (Col1a1 and OPN) (p<0.05) in compared to pure polycaprolactone and (0, 0.5 and 1%) magnesium oxide/polycaprolactone scaffolds. Conclusion: According to the results, it was demonstrated that magnesium oxide/polycaprolactone composite nanofibers have considerable osteoinductive potential, and taking together adipose-derived mesenchymal stem cells-magnesium oxide/polycaprolactone composite nanofibers can be a proper bio-implant to usage for bone regenerative medicine applications. Future in vivo studies are needed to determine this composite therapeutic potential.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Spontaneous Cataract in Nude Athymic Mice

1977 ◽  
Vol 35 ◽  
pp. 512-513
Author(s):  
Ronald H. Bradley ◽  
R. S. Berk ◽  
L. D. Hazlett
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nude Mouse ◽  
Cellular Immunity ◽  
Phosphate Buffer ◽  
Osmium Tetroxide ◽  
Athymic Mice ◽  
Transmission Microscopy ◽  
Mouse Lens ◽  
Scanning Electron

The nude mouse is a hairless mutant (homozygous for the mutation nude, nu/nu), which is born lacking a thymus and possesses a severe defect in cellular immunity. Spontaneous unilateral cataractous lesions were noted (during ocular examination using a stereomicroscope at 40X) in 14 of a series of 60 animals (20%). This transmission and scanning microscopic study characterizes the morphology of this cataract and contrasts these data with normal nude mouse lens.All animals were sacrificed by an ether overdose. Eyes were enucleated and immersed in a mixed fixative (1% osmium tetroxide and 6% glutaraldehyde in Sorenson's phosphate buffer pH 7.4 at 0-4°C) for 3 hours, dehydrated in graded ethanols and embedded in Epon-Araldite for transmission microscopy. Specimens for scanning electron microscopy were fixed similarly, dehydrated in graded ethanols, then to graded changes of Freon 113 and ethanol to 100% Freon 113 and critically point dried in a Bomar critical point dryer using Freon 13 as the transition fluid.

Scanning and Transmission Microscopy of Nematocyst Batteries in Epitheliomuscular Cells of Hydra

1971 ◽  
Vol 29 ◽  
pp. 410-411 ◽  
Author(s):  
Jane A. Westfall ◽  
S. Yamataka ◽  
Paul D. Enos
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Osmium Tetroxide ◽  
External Surface ◽  
Three Dimensional ◽  
Surface Structures ◽  
Transmission Microscopy ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Scanning Electron

Scanning electron microscopy (SEM) provides three dimensional details of external surface structures and supplements ultrastructural information provided by transmission electron microscopy (TEM). Animals composed of watery jellylike tissues such as hydras and other coelenterates have not been considered suitable for SEM studies because of the difficulty in preserving such organisms in a normal state. This study demonstrates 1) the successful use of SEM on such tissue, and 2) the unique arrangement of batteries of nematocysts within large epitheliomuscular cells on tentacles of Hydra littoralis.Whole specimens of Hydra were prepared for SEM (Figs. 1 and 2) by the fix, freeze-dry, coat technique of Small and Màrszalek. The specimens were fixed in osmium tetroxide and mercuric chloride, freeze-dried in vacuo on a prechilled 1 Kg brass block, and coated with gold-palladium. Tissues for TEM (Figs. 3 and 4) were fixed in glutaraldehyde followed by osmium tetroxide. Scanning micrographs were taken on a Cambridge Stereoscan Mark II A microscope at 10 KV and transmission micrographs were taken on an RCA EMU 3G microscope (Fig. 3) or on a Hitachi HU 11B microscope (Fig. 4).

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