scholarly journals SELECTION OF ANTIBIOTIC-PRODUCING STRAINS OF Bacillus spp. ISOLATED FROM TROPICAL FRUITS

2011 ◽  
Vol XVII (4) ◽  
pp. 5-11 ◽  
Author(s):  
Juan Arturo Ragazzo-Sánchez ◽  
◽  
Alejandra Robles-Cabrera ◽  
Leopoldo Lomelí-González ◽  
Guadalupe Luna-Solano ◽  
...  
Food Control ◽  
2021 ◽  
pp. 108119
Author(s):  
Zahoor Ul Hassan ◽  
Roda Al Thani ◽  
Mohammed Alsafran ◽  
Quirico Migheli ◽  
Samir Jaoua

Author(s):  
Dung Thi Thanh Do ◽  
Binh Thanh Le ◽  
Duong Thi Dang Hoang ◽  
Quang Dinh Vo ◽  
Trang Thi Phuong Phan

The aim of this study is to select some Bacillus isolates which are capable of yielding several beneficial enzymes and antagonism to Vibrio parahaemolyticus causing the EMS shrimp disease. In this study, we isolated and screened total of 54 Bacillus isolates from 30 mud, water and shrimp samples at shrimp ponds in Soc Trang province. Among these, 19 isolates were resistant against Vibrio parahaemolyticus strains causing the EMS shrimp disease via two testing methods. Three of them including NA2B13, NA10B2, NA8B1 isolates showed strongresistance and strong one to three kinds of extracellular enzymes to produce. Result of 16S rDNA sequencing and MALDI -TOF showed that NA2B13 and NA8B1 were Bacillus subtilis and NA10B2 was B. amyloliquefaciens. These two species were regarded safe and having potential applications in the production of biological products to prevent EMS shrimp disease.


2013 ◽  
Vol 22 (3) ◽  
pp. 217
Author(s):  
Muhammad Jawal Anwarudinsyah ◽  
Sukartini Sukartini ◽  
Diah Sunarwati ◽  
Rebin Rebin ◽  
Nono Sutrisno

ABSTRAK. Arumanis 143 merupakan varietas mangga unggul nasional yang memiliki kualitas buah cukup baik dan sudah dikenal di pasar, tetapi tingkat produktivitasnya masih rendah dan kulit buah tetap berwarna hijau walaupun sudah masak. Persilangan antara mangga Arumanis dengan varietas yang lebih produktif dan berwarna merah merupakan salah satu cara untuk memperbaiki produktivitas dan mengubah warna menjadi merah. Penelitian dilakukan mulai Januari sampai dengan Desember 2006 di Laboratorium Balai Penelitian Tanaman Buah Tropika Solok dan Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian  untuk menyeleksi secara dini karakter produktif dan warna merah pada kulit buah terhadap 19 semaian hibrida F1 hasil persilangan antara induk Arumanis 143 dengan mangga-mangga Cukurgondang melalui analisis aktivitas enzim esterase dan kandungan antosianin. Hasil penelitian menunjukkan bahwa dari 19 semaian hibrida F1 ada empat semaian yang produktivitasnya lebih rendah dan 15 semaian yang produktivitasnya lebih tinggi dari tetua induk Arumanis 143. Dari 15 semaian ada lima semaian yang sangat produktif karena aktivitas enzim esterasenya lebih tinggi daripada standar (tetua paling produktif yaitu Keith), sehingga dapat dijadikan sebagai kandidat varietas mangga Arumanis yang produktif. Kelima semaian tersebut  berasal dari persilangan  nomor 25, 33, 35, 47, dan 50. Hasil analisis kandungan antosianin menunjukkan bahwa hampir seluruh semaian hibrida F1 memiliki kandungan antosianin lebih tinggi dari induk Arumanis 143, tetapi yang memiliki kandungan antosianin lebih tinggi dari standar (Apel dan Khirsapati Maldah) ada empat semaian, yaitu hibrida dari persilangan nomor 20, 25, 44, dan 48. Keempat  semaian tersebut selanjutnya dikategorikan sebagai kandidat varietas mangga Arumanis yang kulit buahnya berpotensi berwarna merah. Khusus semaian hibrida F1 dari persilangan no 25 selain lebih produktif juga kulit buahnya berpotensi berwarna merah.<br /><br />ABSTRACT. Jawal, M Anwarudin Syah, Sukartini, Sunarwati, D, Rebin, and Sutrisno, N 2012. The Early Selection of Mango Hybrids which Productive and  Red Skin Fruit by Activity of Esterase Enzymes and Anthocyanin Content. The Arumanis 143 is a national superior mango cultivar which have a good quality and marketable, but the low productivity and green of skin fruit. The hybridization of Arumanis 143 cultivar with the productive cultivars and red skin mango is a method to improved the productivity and change the skin color to red on Arumanis cultivar. Early selection on productivity character and red skin color of mango based on esterase enzyme activity and anthocyanin content on hybrid F1 seedling by crossing Arumanis 143 with the productive cultivars and red skin mango. The research was conducted at Laboratory of Indonesian Tropical Fruits Research Institute Solok and Postharvest Research and Development Institute Bogor from January to December 2006. The objective of the research was to early selection of productivity character and red skin color of mango on 19 hybrids F1 by activities of esterase enzyme and anthocyanin content. The results analysis of  esterase enzyme activity showed that there were five seedlings hybrids F1 which activity of esterase enzyme higher than standard (Keith) i.e. hybrid number 25, 33, 35, 47, and 50. Those five seedlings hybrid can be determined as productive candidate of Arumanis mango cultivars. The results analysis of anthocyanin content showed that there were four seedlings hybrids F1 which anthocyanin content higher than standard (Apel and Khirsapati Maldah) i.e. hybrid number 20, 25, 44, and 48. The four seedlings hybrid can be determined as red skin of mango candidate of Arumanis cultivars. The seedlings number 25 have high of esterase enzyme activity and anthocyanin content, thus could be determined as Arumanis cultivars which productive and red skin mango.<br /><br />


1995 ◽  
Vol 46 (5) ◽  
pp. 985 ◽  
Author(s):  
AM Stirling ◽  
LM Coates ◽  
KG Pegg ◽  
AC Hayward

Bacteria and yeasts were isolated from leaves, flowers and fruit of avocado trees that had not been sprayed with pesticides for several years. Of the 1050 microorganisms isolated, 37% inhibited mycelial growth of Colletotrichum gloeosporioides on potato dextrose agar. Many of these organisms also significantly reduced spore germination of the fungus on cellophane overlaying weak sugars agar and a greater proportion of yeasts than bacteria were more effective. Some bacteria and yeasts also reduced spore germination of the pathogen on avocado leaf disks. The predominant group of suppressive bacteria was Bacillus spp., and the antagonistic yeasts included Aureobasidium spp. and a variety of pink and white colony types. Antibiotic resistant isolates of Bacillus, carbendazim resistant isolates of two yeasts and an Aureobasidium sp. were sprayed on avocado leaves and survived for at least 2 months on the phylloplane. On the basis of performance in these tests, isolates with biocontrol and colonization potential were selected and tested for their capacity to provide disease control on fruit. In repeated tests, several bacteria and yeasts consistently reduced lesion development and lesion size on detached avocado fruit when applied prior to inoculating fruit with the pathogen.


2016 ◽  
Vol 38 (3) ◽  
pp. 233 ◽  
Author(s):  
Jéssica Lucinda Saldanha da Silva ◽  
Davi De Holanda Cavalcante ◽  
Fátima Cristiane Teles de Carvalho ◽  
Regine Helena Silva Dos Fernandes Vieira ◽  
Marcelo Vinícius do Carmo e Sá ◽  
...  

The following research isolated and identified the main bacterial groups present in the culture of juvenile Nile tilapia in the presence of bioflocs and/or periphyton. The strains were also tested for the production of exoenzymes, indicative of potential virulence factors, and ability to form biofilm. The water samples were taken from tilapia cultured in the presence of bioflocs (T1), in the presence of bioflocs and periphyton (T2), from traditional culture (T3) and from culture in the presence of periphyton (T4). In the growth and selection of the bacterial groups, pour plate method was used, along with the following media: Plate Count Agar (PCA - DIFCO), Aero Pseudo Selective Agar (GSP - Himedia) and Nutrient Agar (AN - Merck). 46 strains were isolated in the following distribution: T1 (n = 12); T2 (n = 10); T3 (n = 14) and T4 (n = 10). Among the isolates, the most frequent genera were: Pseudomonas spp., Aeromonas spp., Staphylococcus spp., Bacillus spp., Mycobacterium spp., Micrococcus spp., and Corybacterium spp. Bacterial isolates in treatments T1 and T3 tested positive for five virulence profiles each, while those isolated from T2 and T4 for two and three virulence profiles, respectively. Treatments in bioflocs and periphyton (T2) or only periphyton (T4) yielded bacteria of less pathogenic potentials. In relation to the fish growth, T1 and T4 resulted in a higher final weight.  


2021 ◽  
Vol 29 (1) ◽  
pp. 97-106
Author(s):  
Iman Rusmana ◽  
Yuni Puji Hastuti ◽  
Rizal Khoirun Alfisah ◽  
Alina Akhdiya

Certain strains of V. parahaemolyticus carry a gene that encodes a toxin that causes Acute hepatopancreatic necrosis disease (AHPND) in P. vannamei. AHPND attacks shrimp post larvae within 20-30 days after stocking causing up to 100% mortality. The expression of these virulent genes is controlled by the quorum sensing system. This system is inhibited by an anti-quorum sensing (AQS) mechanism. Several Bacillus strains have AQS mechanism by producing AHL-Lactonase enzyme. Therefore, this study aimed to obtain Bacillus spp. having AQS activity for controlling AHPND. The study was conducted from isolation and selection of Bacillus isolates, as well as determination of AQS activity. From 22 samples consisting of shrimp intestines, water and pond sediment samples, a total of 151 isolates of Bacillus spp. were isolated. The screening test for AQS activity obtained 11 isolates that showed AQS activity on Cromobacterium violaceum. Determination of violacein pigment in liquid cultures of C. violaceum showed the index value of the pigment formation was between 0.025-0.166 and 0.026-0.567 at 24-hour and between 48-hour incubations, respectively. The quantitative analysis of violacein production showed that there were six isolates of Bacillus could inhibit the pigment production more than 75%. The isolates were identified as Bacillus cereus (four isolates), Bacillus thuringiensis (one isolate), and Bacillus velezensis (one isolate), respectively. The molecular analysis had confirmed that the isolates have aiiA genes encoding AHL-lactonase enzyme. These Bacillus isolates have potential application for controlling AHNPD disease.


2019 ◽  
Vol 25 (3) ◽  
pp. 247-258
Author(s):  
Dora Ilic ◽  
Ivica Dimkic ◽  
Hadi Waisi ◽  
Panagiotis Gkorezis ◽  
Saud Hamidovic ◽  
...  

Hexavalent chromium, Cr(VI), one of the major pollutants from industrial facilities, is very toxic and harmful for human health and environmental quality. Due to the lack of conventional methods, bioremediation was recommended as an environmentally friendly and effective technique. The aim of this paper was the isolation, identification and selection of the microorganisms which are capable of Cr(VI) reduction in vitro. Heavy metal concentration, detected in four soil samples, within and around the former bicycle factory ?Rog? (Republic of Slovenia), was measured using the ICP-OES method. Bacteria were isolated and tested for chromium tolerance using LB agar supplemented with various Cr(VI) concentrations, whilst Cr(VI) reduction and bacterial growth was determined using the LB liquid medium. From 53 bacterial isolates, five of them showed a tolerance of 1000 mg/L of Cr(VI). Those five isolates showed the capability of growth under various Cr(VI) concentrations (50-1000 mg/L). Initial Cr(VI) concentrations ranging from 50 to 100 mg/L were completely reduced by four bacterial isolates, whilst 500 to 1000 mg/L by Bacillus safensis 342-9. Using 16S rDNA and tuf gene sequence analyses, isolates 270-9R and 342-9 were identified as Bacillus safensis, isolates 351-9 and 270-9C as Bacillus subtilis subsp. subtilis, and 212-9 as Bacillus thuringiensis. These results indicated that these bacteria may be promising tools for remediation of metal-polluted sites.


2012 ◽  
Vol 113 (4) ◽  
pp. 798-806 ◽  
Author(s):  
P. Wongputtisin ◽  
C. Khanongnuch ◽  
W. Khongbantad ◽  
P. Niamsup ◽  
S. Lumyong

Sign in / Sign up

Export Citation Format

Share Document