scholarly journals Aquatic microbiota diversity in the culture of Nile tilapia (Oreochromis niloticus) using bioflocs or periphyton: virulence factors and biofilm formation

2016 ◽  
Vol 38 (3) ◽  
pp. 233 ◽  
Author(s):  
Jéssica Lucinda Saldanha da Silva ◽  
Davi De Holanda Cavalcante ◽  
Fátima Cristiane Teles de Carvalho ◽  
Regine Helena Silva Dos Fernandes Vieira ◽  
Marcelo Vinícius do Carmo e Sá ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Nile Tilapia ◽  
Fish Growth ◽  
Plate Count ◽  
Plate Count Agar ◽  
Bacillus Spp ◽  
Staphylococcus Spp ◽  
Bacterial Groups ◽  
Microbiota Diversity ◽  
Selection Of

The following research isolated and identified the main bacterial groups present in the culture of juvenile Nile tilapia in the presence of bioflocs and/or periphyton. The strains were also tested for the production of exoenzymes, indicative of potential virulence factors, and ability to form biofilm. The water samples were taken from tilapia cultured in the presence of bioflocs (T1), in the presence of bioflocs and periphyton (T2), from traditional culture (T3) and from culture in the presence of periphyton (T4). In the growth and selection of the bacterial groups, pour plate method was used, along with the following media: Plate Count Agar (PCA - DIFCO), Aero Pseudo Selective Agar (GSP - Himedia) and Nutrient Agar (AN - Merck). 46 strains were isolated in the following distribution: T1 (n = 12); T2 (n = 10); T3 (n = 14) and T4 (n = 10). Among the isolates, the most frequent genera were: Pseudomonas spp., Aeromonas spp., Staphylococcus spp., Bacillus spp., Mycobacterium spp., Micrococcus spp., and Corybacterium spp. Bacterial isolates in treatments T1 and T3 tested positive for five virulence profiles each, while those isolated from T2 and T4 for two and three virulence profiles, respectively. Treatments in bioflocs and periphyton (T2) or only periphyton (T4) yielded bacteria of less pathogenic potentials. In relation to the fish growth, T1 and T4 resulted in a higher final weight.  

scholarly journals Microbial assessment of different samples of ostrich (Struthio camelus) and determination of antimicrobial susceptibility profiles of the isolated bacteria

2018 ◽  
Vol 3 (4) ◽  
pp. 437-445
Author(s):  
Israt Jahan ◽  
Nazmi Ara Rumi ◽  
Md Khaled Hossain ◽  
Md Shajedur Rahman ◽  
Md Fakhruzzaman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cloacal Swab ◽  
Plate Count ◽  
Salmonella Spp ◽  
Plate Count Agar ◽  
Antimicrobial Sensitivity ◽  
Bacillus Spp ◽  
Oropharyngeal Swab ◽  
Susceptibility Profiles ◽  
Staphylococcus Spp

The study was conducted to monitor the microbial assessment in oropharyngeal swab, cloacal swab and faecal samples of ostrich and also update knowledge on antimicrobial sensitivity of isolated organisms. The study was performed at Hajee Mohammad Danesh Science & Technology University (HSTU) ostrich farm from January to June, 2016. A total 75 samples were randomly collected from 8 ostriches at different age of which 25 oropharyngeal, 25 cloacal swabs sample and 25 were faeces samples. The organisms were isolated by using standard microbiological method. The result revealed that the average microbial load in plate count agar was highest in faeces (1.48×109±0.27 CFU) than oropharyngeal swab (1.36×109±0.38 CFU) and cloacal swab (1.28×109±0.34 CFU). Among 75 samples, 29 (38.66%) Escherichia coli, 21 (28%) Salmonella spp., 20 (26.67%) Staphylococcus spp. and 5(6.6%) Bacillus spp. were identified. In 25 oropharyngeal swab samples, Staphylococcus spp. was found highest 15 (60%) than other organisms but Escherichia coli were found highest both in cloacal 11(44%) and faecal 12(48%) samples. On antibiogram study Escherichia coli, Salmonella spp., Staphylococcus spp. and Bacillus spp. were more sensitive to Levofloxacin, Azithromycin, Nalidixic Acid and Amoxicillin respectively. While more resistant to Azithromycin, Penicillin, Chloramphenicol and Erythromycin respectively. The obtained results indicated that ostrich excreta are one of the most important sources of Escherichia coli, Staphylococcus spp., Salmonella spp., Bacillus spp. in ostrich farms. Finally, it may be concluding that the logical use of antibiotics must be adopted in ostrich farms reared in Bangladesh for prevention of the appearance of multidrug resistance bacteria. Moreover, proper measures should be taken to aware off zoonotic diseases in peoples who are involved in ostrich farming.Asian J. Med. Biol. Res. December 2017, 3(4): 437-445

scholarly journals Bacterial Assessment of Unpasteurized Fruit Juices Sold in Port Harcourt, Nigeria

2020 ◽  
pp. 1-7
Author(s):  
Constancy Prisca Aleru ◽  
Vivian Nkemkanma Agi ◽  
Kingsley Njoku
Keyword(s):  
Fruit Juices ◽  
Plate Count ◽  
Heterotrophic Plate Count ◽  
E Coli ◽  
Port Harcourt ◽  
Bacillus Spp ◽  
Tiger Nuts ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

This study assessed the bacterial quality of fresh fruit juices sold in some restaurants in Port Harcourt, Nigeria. A total of twenty (20) fruit juice samples were collected from four (4) different types of fruit juices sold at four (4) different restaurants. The fruits that were used for this study are: Orange, Watermelon, Pineapple and Tiger nuts. Samples of the fruit juices were collected for bacteriological assessment using heterotrophic plate count and most probable number techniques, while CHRO Magar for E. coli and other coliforms was used for the detection of E. coli. The results showed that E. coli, Bacillus spp, Klebsiella spp, Streptococcus spp and Staphylococcus spp were present in the fruit juices. The results of the heterotrophic plate count showed that the bacterial count ranged from 2.7 x 102 (Orange) to 7.1 x 103 (Tiger nuts), while the results of the total coliform count ranged from 11 MPN/100 ml to 28 MPN/100 ml. However, the study revealed that the total E. coli count ranged from 0 to 7. The number of E. coli in each of the fruit juices and restaurants ranged from 6 to 13, Bacillus spp 12 to 19, Klebsiella spp 4 to 10, Streptococcus spp 6 to 11 and Staphylococcus spp 4 to 13. Bacillus spp had the highest percentage (31.4%), followed by Staphylococcus spp (20.1%). The highest percentage of bacteria was recovered from Tiger nuts (29.4%), followed by Watermelon (28.4%). Regular monitoring of the quality of fresh fruit juices sold in restaurants in Port Harcourt and other parts of Nigeria should therefore be enforced.

Evaluation of Media for Enumerating Yeasts and Molds in Fresh and Frozen Fruit Purees

1985 ◽  
Vol 48 (4) ◽  
pp. 312-315 ◽  
Author(s):  
L. R. BEUCHAT ◽  
B. V. NAIL
Keyword(s):  
Yeast Extract ◽  
Rose Bengal ◽  
Potato Dextrose Agar ◽  
Plate Count ◽  
High Acid ◽  
Plate Count Agar ◽  
Fungal Populations ◽  
Yeasts And Molds ◽  
Selection Of

Five mycological media were evaluated for their suitability to enumerate yeasts and molds in 11 different fresh and frozen fruit purees. Overall, acidified (pH 3.5) potato dextrose agar (PDA) and oxytetracycline - glucose - yeast extract (OGY, pH 6.5) agar supported highest overall recovery of total fungal populations from purees; plate count agar supplemented with antibiotics and rose bengal - chlortetracycline agar (RBC) were inferior. Dichloran - rose bengal - chloramphenicol agar was superior for restricting spreading of molds, thus facilitating enumeration of colonies. RBC agar was superior to PDA for recovering molds; PDA was superior to RBC for detecting yeasts. It is concluded that selection of acidified PDA and OGY in lieu of other mycological media is appropriate when high-acid food materials such as fruit purees are analyzed for total fungal populations. These media would be exceptionally desirable when test products contain low populations of molds and high populations of yeasts.

Increased activity of soil microorganisms near sclerotia of Sclerotium rolfsii in soil

1971 ◽  
Vol 17 (4) ◽  
pp. 557-562 ◽  
Author(s):  
R. G. Gilbert ◽  
R. G. Linderman
Keyword(s):  
Microbial Activity ◽  
Soil Microorganisms ◽  
Sclerotium Rolfsii ◽  
Bacterial Activity ◽  
Potato Dextrose Agar ◽  
Plate Count ◽  
Plate Count Agar ◽  
Increased Activity ◽  
Selection Of

Microbial activity of soil near sclerotia—"mycosphere" (MS)—increased markedly within 24 h after sclerotia of Sclerotium rolfsii were pressed into the soil, but no changes occurred in the microbial activity of soil not near the sclerotia—non-mycosphere (NMS). Increased microbial activity in the MS, called the "mycosphere effect," was attributed to increased bacterial activity, since numbers of fungi and actinomycetes remained relatively unchanged. The qualitative selection of specific bacteria in the MS by sclerotia was suggested by the increase in bacteria that were tolerant to streptomycin and oxgall bile salts in the plating medium. Exposure of the soil to volatile compounds from alfalfa hay before adding the sclerotia enhanced the rate of the microbial increase in the MS. The energy substrate for the increase in MS organisms was of sclerotial origin and not from culture nutrients adhering to the sclerotia. Addition of concentrated sclerotial leachate to soil, and exposure of soil to alfalfa volatiles also induced an increase in the MS organisms.Bacteria from the MS affected S. rolfsii in vitro more than did bacteria from the NMS. Most of the MS bacteria were inhibitory to S. rolfsii on Difco plate-count agar, had no effect on potato-dextrose agar, and were stimulatory on Czapek-Dox agar. These results suggest that the nutritional status in the MS, as determined by sclerotial exudation or external nutrients, may be of primary importance in determining whether MS organisms will inhibit, will stimulate, or will have no effect on the sclerotia of S. rolfsii.

Evaluation of the antibiotic susceptibility and virulence factors production in Staphylococcus spp. strains used to obtain autologous vaccines

Infectio ro ◽  
2018 ◽  
Vol 2 (54) ◽  
pp. 27
Author(s):  
Mădălina Preda ◽  
Alina Maria Holban ◽  
Lia-Mara Diţu ◽  
Coralia Bleotu ◽  
Mădălina-Maria Muntean ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Antibiotic Susceptibility ◽  
Staphylococcus Spp

The Effectiveness of Amoxicillin and Cefalexin in Two Species from Staphylococcus and Bacillus Isolated from Bovine Mastitis in Sudan

2019 ◽  
Author(s):  
Reem Rabie Mohammed Salih
Keyword(s):  
Bacillus Cereus ◽  
Bovine Mastitis ◽  
Bacillus Coagulans ◽  
Staph Aureus ◽  
Milk Samples ◽  
Sensitivity Tests ◽  
Bacillus Spp ◽  
Staphylococcus Spp

In this study 60 milk samples collected from mastitic cows to isolates and identify bacterial agents. The percentage of isolates was as follows: Staphylococcus spp 52%, Bacillus spp 26%, Enteroccocispp 3%; microccus 3%, Nesseria 2%, Branhamella 3%, Clostridium 1%, Bordetella 2%, Enterobacteria 3%, Aeromonas 4%, Fusobacterium 1%. In sensitivity tests used two antibiotics Amoxicillin and Cephalexin against two highest isolates in this study (Staph spp and Bacillus spp and found: Staph. aureus and Staph. hyicus were resistant to Amoxicllin and cephlaxin relatively, Bacillus coagulans and Bacillus cereus were susceptible to Amoxicllin and cephlaxin.

Selection of Bacillus spp. with decontamination potential on multiple Fusarium mycotoxins

Food Control ◽  
2021 ◽  
pp. 108119
Author(s):  
Zahoor Ul Hassan ◽  
Roda Al Thani ◽  
Mohammed Alsafran ◽  
Quirico Migheli ◽  
Samir Jaoua
Keyword(s):  
Fusarium Mycotoxins ◽  
Bacillus Spp ◽  
Selection Of

scholarly journals Processed By-Products from Soy Beverage (Okara) as Sustainable Ingredients for Nile Tilapia (O. niloticus) Juveniles: Effects on Nutrient Utilization and Muscle Quality

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 590
Author(s):  
Glenise B. Voss ◽  
Vera Sousa ◽  
Paulo Rema ◽  
Manuela. E. Pintado ◽  
Luísa M. P. Valente
Keyword(s):  
Nile Tilapia ◽  
Lactobacillus Rhamnosus ◽  
Protein Digestibility ◽  
Nutrient Utilization ◽  
Nutrient Digestibility ◽  
Fish Growth ◽  
Muscle Quality ◽  
Whole Body ◽  
Hypertrophic Growth ◽  
Bifidobacterium Animalis

The apparent digestibility coefficients (ADCs) of differently processed okara meals were assessed in Nile tilapia diets: dried okara not autoclaved (FOK), dried okara autoclaved (AOK), okara hydrolyzed with Alcalase (ALOK) or Cynara cardunculus proteases (CYOK), and hydrolyzed okara fermented with lactic bacteria: Lactobacillus rhamnosus R11 (CYR11OK) or Bifidobacterium animalis ssp. lactis Bb12 (CYB12OK). Okara processing significantly affected nutrient digestibility: dry matter ADC was highest in CYR11OK (80%) and lowest in FOK (40%). The lowest protein digestibility was observed in CYR11OK (72%), and the highest in AOK (97%) and CYOK (91%), evidencing the effectiveness of the autoclave and the use of C. cardunculus proteases to increase okara protein bioavailability. The inclusion of up to 20% of AOK or CYOK did not affect fish growth, nutrient utilization, or whole body composition of Nile tilapia. The flesh quality (color, pH, water activity, cohesiveness, elasticity and resilience) was not affected by the dietary incorporation of AOK or CYOK. Fish fed with AOK diets stand out for their high density of muscle fibers, particularly in AOK20, which can explain their high muscle firmness and may result in further hypertrophic growth. Altogether, results suggest that hydrolyzed or autoclaved okara are valuable ingredients for Nile tilapia diets.

scholarly journals Exploring the Multimodal Role of Yucca schidigera Extract in Protection against Chronic Ammonia Exposure Targeting: Growth, Metabolic, Stress and Inflammatory Responses in Nile Tilapia (Oreochromis niloticus L.)

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2072
Author(s):  
Zizy I. Elbialy ◽  
Abdallah S. Salah ◽  
Ahmed Elsheshtawy ◽  
Merna Rizk ◽  
Muyassar H. Abualreesh ◽  
...  
Keyword(s):  
Nile Tilapia ◽  
Inflammatory Responses ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Metabolic Stress ◽  
Fish Growth ◽  
Control Group ◽  
Feed Conversion ◽  
Final Body Weight ◽  
Yucca Schidigera

Ammonia is a critical hazardous nitrogen metabolic product in aquaculture. Despite trials for its control, ammonia intoxication remains one of the most critical issues to overcome. In this study, we explored the modulatory effect and potential mechanism by which Yucca schidigera extract (YSE) can ameliorate ammonia intoxication-induced adverse effects on tilapia health and metabolism. A total number of 120 Nile tilapia were evenly assigned into four groups with three replicates each. The first group served as normal control group; the second group was exposed to ammonia alone from the beginning of the experiment and for four weeks. The third group was supplied with YSE in water at a dose of 8 mg/L and exposed to ammonia. The fourth group was supplied with YSE only in water at a dose of 8 mg/L. YSE supplementation succeeded in improving water quality by reducing pH and ammonia levels. Moreover, YSE supplementation markedly alleviated chronic ammonia-induced adverse impacts on fish growth by increasing the final body weight (FBW), specific growth rate (SGR), feed intake and protein efficiency ratio (PER) while reducing the feed conversion ratio (FCR) via improvements in food intake, elevation of hepatic insulin-like growth factor (ILGF-1) and suppression of myostatin (MSTN) expression levels with the restoration of lipid reserves and the activation of lipogenic potential in adipose tissue as demonstrated by changes in the circulating metabolite levels. In addition, the levels of hepato-renal injury biomarkers were restored, hepatic lipid peroxidation was inhibited and the levels of hepatic antioxidant biomarkers were enhanced. Therefore, the current study suggests that YSE supplementation exerted an ameliorative role against chronic ammonia-induced oxidative stress and toxic effects due to its free radical-scavenging potential, potent antioxidant activities and anti-inflammatory effects.

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