Selection of Bacillus spp. with decontamination potential on multiple Fusarium mycotoxins

The aim of this study is to select some Bacillus isolates which are capable of yielding several beneficial enzymes and antagonism to Vibrio parahaemolyticus causing the EMS shrimp disease. In this study, we isolated and screened total of 54 Bacillus isolates from 30 mud, water and shrimp samples at shrimp ponds in Soc Trang province. Among these, 19 isolates were resistant against Vibrio parahaemolyticus strains causing the EMS shrimp disease via two testing methods. Three of them including NA2B13, NA10B2, NA8B1 isolates showed strongresistance and strong one to three kinds of extracellular enzymes to produce. Result of 16S rDNA sequencing and MALDI -TOF showed that NA2B13 and NA8B1 were Bacillus subtilis and NA10B2 was B. amyloliquefaciens. These two species were regarded safe and having potential applications in the production of biological products to prevent EMS shrimp disease.

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Isolation and selection of Bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish

Journal of Applied Microbiology ◽

10.1111/j.1365-2672.2007.03360.x ◽

2007 ◽

Vol 103 (5) ◽

pp. 1471-1479 ◽

Cited By ~ 53

Author(s):

R. Lalloo ◽

S. Ramchuran ◽

D. Ramduth ◽

J. Görgens ◽

N. Gardiner

Keyword(s):

Water Quality ◽

Biological Agents ◽

Ornamental Fish ◽

Bacillus Spp ◽

Selection Of

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Isolation and selection of bacteria and yeasts antagonistic to preharvest infection of avocado by Colletotrichum gloeosporioides

Australian Journal of Agricultural Research ◽

10.1071/ar9950985 ◽

1995 ◽

Vol 46 (5) ◽

pp. 985 ◽

Cited By ~ 3

Author(s):

AM Stirling ◽

LM Coates ◽

KG Pegg ◽

AC Hayward

Keyword(s):

Spore Germination ◽

Colletotrichum Gloeosporioides ◽

Lesion Size ◽

Antibiotic Resistant ◽

Avocado Fruit ◽

Bacillus Spp ◽

Colonization Potential ◽

Aureobasidium Sp ◽

Leaf Disks ◽

Selection Of

Bacteria and yeasts were isolated from leaves, flowers and fruit of avocado trees that had not been sprayed with pesticides for several years. Of the 1050 microorganisms isolated, 37% inhibited mycelial growth of Colletotrichum gloeosporioides on potato dextrose agar. Many of these organisms also significantly reduced spore germination of the fungus on cellophane overlaying weak sugars agar and a greater proportion of yeasts than bacteria were more effective. Some bacteria and yeasts also reduced spore germination of the pathogen on avocado leaf disks. The predominant group of suppressive bacteria was Bacillus spp., and the antagonistic yeasts included Aureobasidium spp. and a variety of pink and white colony types. Antibiotic resistant isolates of Bacillus, carbendazim resistant isolates of two yeasts and an Aureobasidium sp. were sprayed on avocado leaves and survived for at least 2 months on the phylloplane. On the basis of performance in these tests, isolates with biocontrol and colonization potential were selected and tested for their capacity to provide disease control on fruit. In repeated tests, several bacteria and yeasts consistently reduced lesion development and lesion size on detached avocado fruit when applied prior to inoculating fruit with the pathogen.

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Aquatic microbiota diversity in the culture of Nile tilapia (Oreochromis niloticus) using bioflocs or periphyton: virulence factors and biofilm formation

Acta Scientiarum Animal Sciences ◽

10.4025/actascianimsci.v38i3.31910 ◽

2016 ◽

Vol 38 (3) ◽

pp. 233 ◽

Cited By ~ 2

Author(s):

Jéssica Lucinda Saldanha da Silva ◽

Davi De Holanda Cavalcante ◽

Fátima Cristiane Teles de Carvalho ◽

Regine Helena Silva Dos Fernandes Vieira ◽

Marcelo Vinícius do Carmo e Sá ◽

...

Keyword(s):

Virulence Factors ◽

Nile Tilapia ◽

Fish Growth ◽

Plate Count ◽

Plate Count Agar ◽

Bacillus Spp ◽

Staphylococcus Spp ◽

Bacterial Groups ◽

Microbiota Diversity ◽

Selection Of

The following research isolated and identified the main bacterial groups present in the culture of juvenile Nile tilapia in the presence of bioflocs and/or periphyton. The strains were also tested for the production of exoenzymes, indicative of potential virulence factors, and ability to form biofilm. The water samples were taken from tilapia cultured in the presence of bioflocs (T1), in the presence of bioflocs and periphyton (T2), from traditional culture (T3) and from culture in the presence of periphyton (T4). In the growth and selection of the bacterial groups, pour plate method was used, along with the following media: Plate Count Agar (PCA - DIFCO), Aero Pseudo Selective Agar (GSP - Himedia) and Nutrient Agar (AN - Merck). 46 strains were isolated in the following distribution: T1 (n = 12); T2 (n = 10); T3 (n = 14) and T4 (n = 10). Among the isolates, the most frequent genera were: Pseudomonas spp., Aeromonas spp., Staphylococcus spp., Bacillus spp., Mycobacterium spp., Micrococcus spp., and Corybacterium spp. Bacterial isolates in treatments T1 and T3 tested positive for five virulence profiles each, while those isolated from T2 and T4 for two and three virulence profiles, respectively. Treatments in bioflocs and periphyton (T2) or only periphyton (T4) yielded bacteria of less pathogenic potentials. In relation to the fish growth, T1 and T4 resulted in a higher final weight.

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Effect of Wheat Cultivar on the Concentration of Fusarium Mycotoxins in Wheat Stems

Plant Disease ◽

10.1094/pdis-12-17-2034-re ◽

2018 ◽

Vol 102 (12) ◽

pp. 2539-2544 ◽

Cited By ~ 2

Author(s):

Kaitlyn M. Bissonnette ◽

Frederic L. Kolb ◽

Keith A. Ames ◽

Carl A. Bradley

Keyword(s):

Wheat Cultivar ◽

Effective Control ◽

Wheat Cultivars ◽

Fusarium Mycotoxins ◽

Head Blight ◽

Fusarium Mycotoxin ◽

Fhb Resistance ◽

Index Value ◽

Moderately Resistant ◽

Selection Of

Effective control of Fusarium-mycotoxin accumulation in grain affected by Fusarium head blight (FHB) (caused by Fusarium graminearum) begins with selecting moderately resistant wheat cultivars; however, little is known about how this resistance affects mycotoxin levels in the stem. A study was conducted from 2011 to 2014 in a mist-irrigated FHB nursery in Urbana, IL to determine whether the FHB resistance class of a cultivar (very susceptible, susceptible, moderately susceptible, and moderately resistant) affects the concentration of Fusarium mycotoxins in the stem. FHB incidence, FHB severity, and Fusarium-damaged kernel ratings were collected and used to calculate FHB index; incidence, severity, and kernel damage (ISK) index; and deoxynivalenol (DON), incidence, severity, and kernel damage (DISK) index. Grain was assayed for levels of DON, and the bottom 25 cm of plant stems was collected from each plot and assayed for DON, 3-acetyl-deoxynivalenol (3ADON), and 15-acetyl-deoxynivalenol (15ADON). Significant differences in DON concentration in the grain were detected among cultivars (P = 0.0001) and for the concentration of all DON (P = 0.003), 3ADON (P = 0.03), and 15ADON (P < 0.0001) in the stem. Significant differences among resistance classes were observed for FHB index value (P < 0.0001), ISK index (P = 0.006), and DISK index (P = 0.004). In all years of this study, the concentration of DON in the grain and the concentrations of all mycotoxins in the stem were consistently lower in the moderately resistant cultivars. All three indices were poor indicators of mycotoxin concentrations in the stem. Overall, the selection of a moderately resistant cultivar provides effective control of DON accumulation in the grain and mycotoxin accumulation in the stem.

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Characteristics and Activity Anti Qourum Sensing Bacillus spp. Isolated from Penaeus vannamei Shrimp Ponds

HAYATI Journal of Biosciences ◽

10.4308/hjb.29.1.97-106 ◽

2021 ◽

Vol 29 (1) ◽

pp. 97-106

Author(s):

Iman Rusmana ◽

Yuni Puji Hastuti ◽

Rizal Khoirun Alfisah ◽

Alina Akhdiya

Keyword(s):

Quorum Sensing ◽

Pigment Formation ◽

Shrimp Ponds ◽

Genes Encoding ◽

Bacillus Spp ◽

Violacein Production ◽

Index Value ◽

Acute Hepatopancreatic Necrosis Disease ◽

Selection Of

Certain strains of V. parahaemolyticus carry a gene that encodes a toxin that causes Acute hepatopancreatic necrosis disease (AHPND) in P. vannamei. AHPND attacks shrimp post larvae within 20-30 days after stocking causing up to 100% mortality. The expression of these virulent genes is controlled by the quorum sensing system. This system is inhibited by an anti-quorum sensing (AQS) mechanism. Several Bacillus strains have AQS mechanism by producing AHL-Lactonase enzyme. Therefore, this study aimed to obtain Bacillus spp. having AQS activity for controlling AHPND. The study was conducted from isolation and selection of Bacillus isolates, as well as determination of AQS activity. From 22 samples consisting of shrimp intestines, water and pond sediment samples, a total of 151 isolates of Bacillus spp. were isolated. The screening test for AQS activity obtained 11 isolates that showed AQS activity on Cromobacterium violaceum. Determination of violacein pigment in liquid cultures of C. violaceum showed the index value of the pigment formation was between 0.025-0.166 and 0.026-0.567 at 24-hour and between 48-hour incubations, respectively. The quantitative analysis of violacein production showed that there were six isolates of Bacillus could inhibit the pigment production more than 75%. The isolates were identified as Bacillus cereus (four isolates), Bacillus thuringiensis (one isolate), and Bacillus velezensis (one isolate), respectively. The molecular analysis had confirmed that the isolates have aiiA genes encoding AHL-lactonase enzyme. These Bacillus isolates have potential application for controlling AHNPD disease.

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Reduction of hexavalent chromium by Bacillus spp. isolated from heavy metals-polluted soil

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq180607003i ◽

2019 ◽

Vol 25 (3) ◽

pp. 247-258

Author(s):

Dora Ilic ◽

Ivica Dimkic ◽

Hadi Waisi ◽

Panagiotis Gkorezis ◽

Saud Hamidovic ◽

...

Keyword(s):

Hexavalent Chromium ◽

Heavy Metal Concentration ◽

Bacterial Isolates ◽

Industrial Facilities ◽

Bacillus Safensis ◽

Bacillus Spp ◽

Polluted Sites ◽

Chromium Tolerance ◽

Selection Of

Hexavalent chromium, Cr(VI), one of the major pollutants from industrial facilities, is very toxic and harmful for human health and environmental quality. Due to the lack of conventional methods, bioremediation was recommended as an environmentally friendly and effective technique. The aim of this paper was the isolation, identification and selection of the microorganisms which are capable of Cr(VI) reduction in vitro. Heavy metal concentration, detected in four soil samples, within and around the former bicycle factory ?Rog? (Republic of Slovenia), was measured using the ICP-OES method. Bacteria were isolated and tested for chromium tolerance using LB agar supplemented with various Cr(VI) concentrations, whilst Cr(VI) reduction and bacterial growth was determined using the LB liquid medium. From 53 bacterial isolates, five of them showed a tolerance of 1000 mg/L of Cr(VI). Those five isolates showed the capability of growth under various Cr(VI) concentrations (50-1000 mg/L). Initial Cr(VI) concentrations ranging from 50 to 100 mg/L were completely reduced by four bacterial isolates, whilst 500 to 1000 mg/L by Bacillus safensis 342-9. Using 16S rDNA and tuf gene sequence analyses, isolates 270-9R and 342-9 were identified as Bacillus safensis, isolates 351-9 and 270-9C as Bacillus subtilis subsp. subtilis, and 212-9 as Bacillus thuringiensis. These results indicated that these bacteria may be promising tools for remediation of metal-polluted sites.

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