scholarly journals BS1.4 - Graphene Hybrids: Functionalized Nanomaterial for Single-step Immunoassay Based on Fluorescence Quenching and Size Separation

2018 ◽  
Author(s):  
A. Shirai ◽  
K. Sueyoshi ◽  
T. Endo ◽  
H. Hisamoto
Keyword(s):  
Fluorescence Quenching ◽  
Single Step ◽  
Size Separation

Development of a single-step immunoassay microdevice based on a graphene oxide-containing hydrogel possessing fluorescence quenching and size separation functions

The Analyst ◽  
2017 ◽  
Vol 142 (3) ◽  
pp. 472-477 ◽  
Author(s):  
Akihiro Shirai ◽  
Kaho Nakashima ◽  
Kenji Sueyoshi ◽  
Tatsuro Endo ◽  
Hideaki Hisamoto
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Single Step ◽  
Size Separation ◽  
Microchannel Array ◽  
Pdms Microchannel ◽  
Peg Coating ◽  
Separation Functions

An immunoassay was successfully integrated into a “single-step” by developing a microdevice composed of a GO-containing hydrogel and a PDMS microchannel array with a PEG coating containing a fluorescently-labelled antibody.

Fast and single-step immunoassay based on fluorescence quenching within a square glass capillary immobilizing graphene oxide–antibody conjugate and fluorescently labelled antibody

The Analyst ◽  
2016 ◽  
Vol 141 (11) ◽  
pp. 3389-3394 ◽  
Author(s):  
Akihiro Shirai ◽  
Terence G. Henares ◽  
Kenji Sueyoshi ◽  
Tatsuro Endo ◽  
Hideaki Hisamoto
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Single Step ◽  
Glass Capillary ◽  
Antibody Conjugate ◽  
Ultrafast Measurement

A single-step immunoassay device for ultrafast measurement of proteins within 40 seconds, fabricated by using a square glass capillary, antibody–graphene oxide conjugate and fluorescently labelled antibody, was developed.

Homogeneous DNA Detection Based on Fluorescence Quenching by Nanoparticles in Single-step Format: Target-Induced Configuration Transform

2009 ◽  
Vol 27 (3) ◽  
pp. 523-528 ◽  
Author(s):  
Songbai ZHANG ◽  
Zaisheng WU ◽  
Ming XIE ◽  
Guoli SHEN ◽  
Ruqin YU
Keyword(s):  
Fluorescence Quenching ◽  
Dna Detection ◽  
Single Step

V884: Demonstration of a Novel Single-Step Percutaneous Access Sheath for Nephrostolithotomy: A Video Presentation

2005 ◽  
Vol 173 (4S) ◽  
pp. 240-240
Author(s):  
Premal J. Desai ◽  
David A. Hadley ◽  
Lincoln J. Maynes ◽  
D. Duane Baldwin
Keyword(s):  
Single Step ◽  
Video Presentation ◽  
Percutaneous Access ◽  
Access Sheath

Effect of Lipoprotein(a) and LDL on Plasminogen Binding to Extracellular Matrix and on Matrix-dependent Plasminogen Activation by Tissue Plasminogen Activator

1996 ◽  
Vol 75 (03) ◽  
pp. 497-502 ◽  
Author(s):  
Hadewijch L M Pekelharing ◽  
Henne A Kleinveld ◽  
Pieter F C.C.M Duif ◽  
Bonno N Bouma ◽  
Herman J M van Rijn
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Plasminogen Activator ◽  
Binding Sites ◽  
Umbilical Vein ◽  
Single Step ◽  
Plasminogen Activation ◽  
Structural Homology ◽  
Tissue Plasminogen ◽  
Umbilical Vein Endothelial Cells

SummaryLp(a) is an LDL-like lipoprotein plus an additional apolipoprotein apo(a). Based on the structural homology of apo(a) with plasminogen, it is hypothesized that Lp(a) interferes with fibrinolysis. Extracellular matrix (ECM) produced by human umbilical vein endothelial cells was used to study the effect of Lp(a) and LDL on plasminogen binding and activation. Both lipoproteins were isolated from the same plasma in a single step. Plasminogen bound to ECM via its lysine binding sites. Lp(a) as well as LDL were capable of competing with plasminogen binding. The degree of inhibition was dependent on the lipoprotein donor as well as the ECM donor. When Lp(a) and LDL obtained from one donor were compared, Lp(a) was always a much more potent competitor. The effect of both lipoproteins on plasminogen binding was reflected in their effect on plasminogen activation. It is speculated that Lp(a) interacts with ECM via its LDL-like lipoprotein moiety as well as via its apo(a) moiety.

Purification of Urokinase from Complex Mixtures Using Immobilized Monoclonal Antibody Against Urokinase Light Chain

1983 ◽  
Vol 49 (01) ◽  
pp. 024-027 ◽  
Author(s):  
David Vetterlein ◽  
Gary J Calton
Keyword(s):  
Monoclonal Antibody ◽  
Light Chain ◽  
Culture Media ◽  
Biological Fluids ◽  
Single Step ◽  
High Yield ◽  
Step Method ◽  
Commercial Preparation ◽  
E Coli ◽  
Tissue Culture Media

SummaryThe preparation of a monoclonal antibody (MAB) against high molecular weight (HMW) urokinase light chain (20,000 Mr) is described. This MAB was immobilized and the resulting immunosorbent was used to isolate urokinase starting with an impure commercial preparation, fresh urine, spent tissue culture media, or E. coli broth without preliminary dialysis or concentration steps. Monospecific antibodies appear to provide a rapid single step method of purifying urokinase, in high yield, from a variety of biological fluids.

Distribution of Aedes aegypti and Aedes albopictus in different eco zones of Thiruvananthapuram city with special reference to dengue viremia in humans

ENTOMON ◽  
2018 ◽  
Vol 43 (4) ◽  
pp. 223-230
Author(s):  
S. Sunil Kumar ◽  
D.A. Evans ◽  
K. Muthulakshmi ◽  
T. DilipKumar ◽  
R. Heera Pillai ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Dengue Shock Syndrome ◽  
Single Step ◽  
Equal Distribution ◽  
Distribution Study ◽  
Urban Zone ◽  
High Prevalence ◽  
Vector Management ◽  
Efficient Vector

Mosquito index study of three ecologically different ecozones of the Thiruvananthapuram district, Kerala showed sharp difference on the proportionate distribution of Aedes aegypti and Aedes albopictus. Human dengue viremia (HDV) was very high in those ecozones where A.aegypti density was high and HDV was low where A.albopictus was high. In a coastal zone of Thiruvananthapuram city, A. aegypti was the most abundant vector and in a hilly, arid suburban zone, A.albopictus was the abundant vector. In the urban zone both species of mosquitoes showed equal distribution. Study on the circulating serotypes in the serum of HDV by Single step single tube Multiplex PCR showed all the four serotypes viz DENV1, DENV2, DENV3 and DENV4 in patients of Thiruvananthapuram city, which indicated the possibility of Dengue Shock Syndrome, unless there is efficient vector management. Among the four dengue serotypes, Type 1 was the most abundant virus. Abundance of microhabitats in Thiruvananthapuram city, which support A. aegypti may be the reason for high prevalence of dengue fever in the urban zone.

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