scholarly journals Spermatozoa Survival Rate in Stored Cock Semen Extended With Diluent Fortified With Natural Antioxidant Sources under Room Temperature

2021 ◽  
Vol 43 (2) ◽  
pp. 100-106
Author(s):  
E. O Ewuola ◽  
A. S Balogun ◽  
A. A Lawanson
Keyword(s):  
Sperm Motility ◽  
Orange Juice ◽  
Natural Antioxidants ◽  
Egg Yolk ◽  
Coconut Water ◽  
Control Treatment ◽  
Sperm Cells ◽  
Mass Activity ◽  
Live Sperm

An experiment was conducted in a completely randomized design with five treatments in triplicates. Four extenders were formulated: Lake Extender; Coconut water-Orange juice Extender (CWOE); Egg yolk-Tomato juice Extender (EYTE) and Milk based-Carrot juice Extender (MCE), were used to extend cock semen constituting treatments 2, 3, 4 and 5 respectively, while the control (Treatment 1) was unextended semen. Semen was harvested from 8 proven Isa breeder cocks aged 35-40 weeks, pooled and evaluated. The ejaculate was divided into 5 and each part was extended with the extenders at ratio 1:2 (Semen: Extender). The extended and un-extended semen samples were kept under room temperatures (26.6-27.4oC) with relative humidity of 64.0-74.0% for in vitro assessment at 2 hrs interval until percentage motility was less than 60%. The results showed that the mass activity, sperm motility and percentage livability reduced p<0.05) as the time of storage increased. Mass activities of unextended semen and the one extended with coconut water-orange juice were significantly (P<0.05) higher than that of other treatments at 0 hour period of storage. Sperm motility in treatments 1 (95.0%), 3 (93.3%) and 4 (88.3%) was not significantly (p>0.05) different from one another, but they was significantly (p<0.05) higher than treatments 2 (40.0%) and 5 (71.7%). At 2 hours of storage, the mass activity and percentage sperm motility in treatment 2 were significantly (P<0.05) lower than other treatments, while live sperm cells were significantly lower for Treatment 3 than other treatments. At 4 hours of storage, mass activity and motility were zero in Treatment 2, while Treatment 1 recorded the highest values (66.7% and 93.5% respectively). Percent motility in Treatments 3, 4 and 5 were 55.0, 50.0 and 43.3%, respectively. However, percent live sperm cells were significantly (P<0.05) higher in treatments 2, 4, 5 and 1 than In treatment 3. Both CWOE and EYTE possessed a good keeping quality for semen storage. The extenders fortified with natural antioxidants can be used for on-farm insemination in poultry within two hours of collection.

scholarly journals Spermatozoa Survival Rate in Stored Cock Semen Extended With Diluent Fortified With Natural Antioxidant Sources under Room Temperature

2021 ◽  
Vol 43 (1) ◽  
pp. 100-106
Author(s):  
E. O. Ewuola ◽  
A. S. Balogun ◽  
A. A. Lawanson
Keyword(s):  
Sperm Motility ◽  
Orange Juice ◽  
Natural Antioxidants ◽  
Egg Yolk ◽  
Coconut Water ◽  
Control Treatment ◽  
Sperm Cells ◽  
Mass Activity ◽  
Live Sperm

An experiment was conducted in a completely randomized design with five treatments in triplicates. Four extenders were formulated: Lake Extender; Coconut water-Orange juice Extender (CWOE), Egg yolk-Tomato juice Extender (EYTE) and Milk based-Carrot Extender (MCE), were used to extend cock semen constituting treatments 2, 3, 4 and 5 respectively, while the control (Treatment 1) was unextended semen. Semen was harvested from 8 proven Isa breeder cocks aged 35-40 weeks, pooled and evaluated. The ejaculate was divided into 5 and each part was extended with the extenders at ratio 1:2 (Semen: Extender). The extended and un-extended semen samples were kept under room temperatures (26.6 27.4°C) with relative humidity of 64.0-74.0% for in vitro assessment at 2 hrs interval until percentage motility was less than 60%. The results showed that the mass activity, sperm motility and percentage livability reduced p<0.05) as the time of storage increased. Mass activities of unextended semen and the one extended with coconut water-orange juice were significantly (P<0.05) higher than that of other treatments at 0 hour period of storage. Sperm motility in treatments 1 (95.0%), 3 (93.3%) and 4 (88.3%) was not significantly (p>0.05) different from one another, but they was significantly (p<0.05) higher than treatments 2 (40.0%) and 5 (71.7%). At 2 hours of storage, the mass activity and percentage sperm motility in treatment 2 were significantly (P<0.05) lower than other treatments, while live sperm cells were significantly lower for Treatment 3 than other treatments. At 4 hours of storage, mass activity and motility were zero in Treatment 2, while Treatment 1 recorded the highest values (66.7% and 93.5% respectively). Percent motility in Treatments 3, 4 and 5 were 55.0, 50.0 and 43.3%, respectively. However, percent live sperm cells were significantly (P<0.05) higher in treatments 2, 4, 5 and 1 than the in treatment 3. Both CWOE and EYTE possessed a good keeping quality for semen storage. The extenders fortified with natural antioxidants can be used for on-farm insemination in poultry within two hours of collection.

scholarly journals Effects of apple and orange juices on quality of refrigerated goat semen

2018 ◽  
Vol 63 (1) ◽  
pp. 53-65
Author(s):  
Ezekiel Adekunle ◽  
James Daramola ◽  
Olusiji Sowande ◽  
John Abiona ◽  
Monsuru Abioja
Keyword(s):  
Sperm Motility ◽  
Orange Juice ◽  
Apple Juice ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Fruit Juices ◽  
Sperm Viability ◽  
In Vitro Storage

This study investigated the effects of apple and orange juices on quality of refrigerated spermatozoa of goat bucks. Semen samples from WAD goat bucks were diluted with Tris-egg yolk extenders each supplemented with apple and orange juices at 0, 2.5, 5, 7.5 and 10/100 ml of diluents. The diluted semen samples were assessed for sperm viability and malondialdehyde (MDA) concentration after in vitro storage for 240 hours at 5oC. The ability to maintain sperm motility was higher in the extenders with 7.5% orange juice followed by 10% apple juice compared to other treatments (P<0.05). The extenders supplemented with 2.5%, 5% and 7.5% apple juice, and 5% orange juice had higher intact acrosome compared to other treatments and the control (P<0.05). The 10% orange juice had higher percentage membrane integrity compared to other treatments. Consistent and reduced (P<0.05) MDA levels were observed in the extenders supplemented with fruit juices and lower MDA was observed in the extenders supplemented with 10% apple juice compared to other treatments and the control (P<0.05). The findings reveal that additions of the fruit juices to semen extenders to maintain the viability of refrigerated spermatozoa were best at concentrations of 10 ml/100 ml of apple juice and 7.5 ml/100 ml of orange juice.

scholarly journals The impact of adding different levels of egg yolk on the motility and morphology pre and post thaw cryopreservation of goat semen

2019 ◽  
Vol 12 (1) ◽  
pp. 107-115
Author(s):  
Nawfal Mutlak
Keyword(s):  
Liquid Nitrogen ◽  
Breeding Season ◽  
Sperm Motility ◽  
Egg Yolk ◽  
Sperm Cells ◽  
Mass Activity ◽  
Semen Extender ◽  
Motility Of Sperm ◽  
The Impact ◽  
Different Levels

The aim of this study was to examine the effect of different concentrations of egg yolk EY (0%, 10%, and 20%) in the semen extender during the cryopreservation process of goat semen out of the breeding season. A total of 12 ejaculates were collected from six Anglo Nubain dairy bucks as two ejaculates for each buck aged between (1-5) years over a two week period by using Electro-ejaculation (EEJ) during the non-breeding season. Post collection, the semen samples were evaluated for motility and mass activity. Subsequently, the semen samples were initially diluted in Tris solution (without Egg yolk or Glycerol) in order to preserve the motility of sperm cells. The semen samples from each buck were evaluated for pre-freezing motility and morphology then divided into three sub-samples and diluted in Tris extender with T1 (control) 0% EY, T2 10% EY, and T3 20% EY. The semen samples were frozen in liquid nitrogen (-196 C). After thawing, the semen samples were evaluated for sperm motility and morphology. The morphology of sperm did not differ among treatments nor between pre-freezing and post-thawing evaluations. However, the motility of semen diluted with 10% EY was (P<0.05) numerically but not statistically higher than semen diluted with 0% and 20% EY. According to the obtained results of this study, it is recommended that a 10% EY level or less be included in the Tris extender during cryopreservation of goat semen for superior motility and morphology results.

scholarly journals Ram and Goat Semen Immunosexed and Diluted in Powdered Coconut Water-Based Preservation Medium (ACP101/102c).

2021 ◽  
Vol 49 ◽  
Author(s):  
Bruna Farias Brito ◽  
Bárbara Mara Bandeira Santos ◽  
Leonardo Alves Rodrigues Cabral ◽  
Luiz Carlos Pinheiro Maia ◽  
Natanael Aguiar Braga Negreiros ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Specific Protein ◽  
Coconut Water ◽  
In Vivo Studies

Background: Sperm sexing aims to separate sperm populations in carriers of the “X” or “Y” chromosome. Currently, flow cytometry is a technique that allows greater accuracy; however, it causes structural changes in sperm, reduces viability, and has a high cost. As a result, other methods have been researched, including immunosexing, which uses monoclonal antibodies to detect sex-specific surface antigens. Thus, the objective of this study was to evaluate the immunosexing technique using a monoclonal antibody against sex-specific protein (HY) in the conservation of ram and goat semen in ACP101/102c.Materials, Methods & Results: Ejaculates from 5 rams and 5 goats were collected with the aid of an artificial vagina; they were evaluated and submitted to the immunosexing protocol, according to the manufacturer's recommendations, using the Monoclonal Antibody Kit specific for mammalian sperm with “Y” chromosomes (HY; HY Biotechnology, Rio de Janeiro, RJ, Brazil). After sexing, the supernatant was resuspended in the cryopreservation diluent: ACP ram (ACP101/102c + 20% egg yolk + 7% glycerol) and ACP goat (ACP101/102c + 2.5% egg yolk + 7% glycerol), packaged in 0.25 mL straws, refrigerated at 4°C, stabilized for 30 min, frozen in liquid nitrogen vapor (-60°C) for 15 min, immersed in liquid nitrogen, and stored in cryogenic cylinders. The samples were evaluated in natura (T1), after immunosexing (T2) and after thawing (T3) for sperm motility subjectively using conventional microscopy (40x). Plasma membrane integrity (IMP) and sperm cell morphology were evaluated by the smear staining technique using eosin-nigrosine dye, and the percentages of healthy and morphologically defect spermatozoa were determined. In the evaluation of ram semen regarding sperm motility and IMP, no statistically significant differences were observed between treatments after sexing in the evaluation of absolute data (P > 0.05), with the difference being observed only between T1 and T2, and T3 (P < 0.05). Regarding the relative percentage and sperm morphology, no statistically significant differences were observed (P > 0.05). Regarding the evaluation of goat semen samples, the motility parameters were consistent with the technique submitted; however, the IMP data did not appear as expected, requiring further evaluation for a better assessment of the technique for this species.Discussion: The data obtained from ram semen submitted to the immunosexing protocol, regarding the absolute evaluation of motility and IMP, demonstrated that the non-sexed semen (T1) was superior to the sexed treatments (T2 and T3); however, it is noteworthy that freezing started with approximately 50% of the cells, since the immunosexing technique results in a loss of viability of approximately 50% of the sperm, which corresponds to the ratio of sperm carrying the X chromosome. In addition, when the data in this study were transformed into relative values, no statistical differences were observed, indicating that the immunosexing protocol, as well as the freezing protocol, did not significantly affect the quality of ram sperm cells. In relation to the immunosexing of goat semen, future studies should be conducted in vitro to define a more appropriate protocol for the species and, in addition, in vivo studies should be performed to prove the quality of the technique. It was concluded that the immunosexing process using a monoclonal antibody against sex-specific protein (HY) associated with the use of powdered coconut water diluent (ACP101/102c) in the cryopreservation of semen proved to be efficient in the in vitro evaluation of ovine species.

Kriopreservasi Semen Kambing Boer dengan Konsentrasi Pengencer Nira Aren dan Gliserol Berbeda

2019 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Muhammad Riyadhi ◽  
Anis Wahdi ◽  
Muhammad Rizal
Keyword(s):  
Sperm Motility ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Boer Goat ◽  
Palm Juice ◽  
Sperm Membrane ◽  
Live Sperm

ABSTRAK                                                                        Penelitian bertujuan untuk mengetahui efektivitas nira aren sebagai pengencer alternatif dalam proses pembekuan (kriopreservasi) semen kambing boer.Kriopreservasi semen kambing boer menggunakan pengencer tris-gliserol-kuning telur (P1 73-7-20%), nira aren-gliseol-kuning telur(masing-masing P2 74-6-20%, P3 73-7-20%, dan P4 72-8-20%) dan andromed (P5 tanpa mengandung kuning telur dan gliserol). Parameter evaluasi meliputi motilitas, viabilitas, dan membrane plasma utuh setelah pengenceran, ekuilibrasi dan thawing.  Evaluasi motilitas pasca thawing menunjukkan P5 52% berbeda nyata (P<0.05) dengan P1 42%, selanjutnya P5 dan P1 berbeda sangat nyata (P<0.05) dengan P2 8%, P3 6% dan P4 12%.  Viabilitas pasca thawing menunjukkan P5 65,4% tidak berbeda nyata (P>0,05) dengan P1 61,8%, akan tetapi P5 dan P1 berbeda sangat nyata (P<0.05) dengan P2 26,2%, P3 29,8%, dan P4 34%.  Membran plasma utuh (MPU) pasca thawing menunjukkan P5 66,2% tidak berbeda nyata (P>0,05) dengan P1 65,4%, akan tetapi keduanya berbeda sangat nyata (P<0.05) dengan P2 39%, P3 38%, dan P4 36,2%.  Disimpulkan kriopreservasi semen kambing boer dengan pengencer nira aren dan gliserol pada konsentrasi berbeda belum dapat dipergunakan sebagai sumber bibit berdasarkan standar nasional Indonesia.Kata Kunci : Kambing boer, semen, nira arenABSTRACTThe experiment was conducted to determine the effect of sugar palm juice as alternative extender for cryopreservation process of boer semen.Tris-glycerol-egg yolk (P1 73-7-20%), Sugar palm juice-glyserol-egg yolk (P2 74-6-20%, P373-7-20%, dan P4 72-8-20%), and andromed (P5) used as a extender  in the cryopreservation process of boer semen.  Sperm motility (%), live sperm (%) and sperm membrane integrity (%) were recorded after diluted, equilibration and freeze-thawing.  Result of post thawing motility showed that P5 52% was significantly different (P <0.05) with P1 42%, then P5 and P1 were significantly different (P <0.05) with P2 8%, P3 6% and P4 12%. Viability after thawing showed P5 65.4% was not significantly different (P> 0.05) with P1 61.8%, but P5 and P1 significantly different (P <0.05) with P2 26.2%, P3 29.8 %, and P4 34%. Spermmembrane integrity post-thawing showed P5 66.2% was not significantly different (P> 0.05) with P1 65.4%, but both were very significantly different (P <0.05) with P2 39%, P3 38% and P4 36.2%. Conclusions, sugar palm juice-glycerol-egg yolk with differentconcentrationsineffectively as an alternative extenderin cryopreservation of boer semen.Keywords: boer goat, semen, sugar palm juice

scholarly journals Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations

2015 ◽  
Vol 36 (6) ◽  
pp. 3699
Author(s):  
Rodrigo Arruda de Oliveira ◽  
Marco Antônio De Oliveira Viu ◽  
Maria Lúcia Gambarini
Keyword(s):  
Lipid Peroxidation ◽  
Sperm Motility ◽  
Membrane Integrity ◽  
Membrane Lipid ◽  
Sperm Cells ◽  
Sperm Viability ◽  
Membrane Lipid Peroxidation ◽  
Acrosomal Membrane ◽  
In Vitro Effects

Handling equine semen during the refrigeration process reduces sperm viability, and consequently causes membrane lipid peroxidation, among other challenges. The present study aimed to evaluate the in vitro effects of glutathione (control, 1. 0, 1. 5, and 2. 5 mM) on equine semen in a refrigeration protocol of 16ºC for 36 hours. The following variables were evaluated after 0, 12, 24, and 36 hours refrigeration: total sperm motility, vigor, viability, and plasma and acrosomal membrane integrity. Motility was higher with 2. 5mM of glutathione (57. 8 ± 7. 3) after 12 hours of refrigeration compared to the control (53. 2 ± 8. 3) (P < 0. 05). After 36 hours of refrigeration, motility was higher with 1. 5 mM (43. 4 ± 12. 7) and 2. 5mM glutathione (45. 5 ± 6. 2), than it was with 1mM glutathione (38. 2 ± 9) and the control (35. 5 ± 18. 4) (P < 0. 05), respectively. Vigor was highest with 1. 5mM glutathione (3. 7 ± 0. 3) after 36 hours compared to the control (3. 2 ± 1. 1), (P < 0. 05). Viability differed between control and 1mM treatments (79. 5 ± 1. 8) only after 24 hours (75. 5 ± 9. 7) (P < 0. 05). Throughout the investigation, no significant differences were noted in plasma and acrosomal membrane integrity (P > 0. 05). The 1. 5 and 2. 5mM glutathione levels were more efficient in protecting sperm cells and yielded higher total motility values after 36 hours of refrigeration.

284 COMPARATIVE FERTILITY OF FRESHLY-COLLECTED VERSUS FROZEN - THAWED SPERMATOZOA FOR IN VITRO FERTILIZATION IN THE FISHING CAT (PRIONAILURUS VIVERRINUS)

2006 ◽  
Vol 18 (2) ◽  
pp. 249
Author(s):  
G. Magarey ◽  
J. Herrick ◽  
K. Thiangtum ◽  
W. Tunwattana ◽  
W. Swanson
Keyword(s):  
Sperm Motility ◽  
Culture Medium ◽  
Culture Media ◽  
Egg Yolk ◽  
Ovarian Follicles ◽  
Blastocyst Stage ◽  
Motile Sperm ◽  
Vitro Fertilization ◽  
International Transport

Wild populations of fishing cats (Prionailurus viverrinus) in Southeast Asia are in decline, primarily due to habitat loss. Because the fishing cat population in North American zoos is small (n = 69) and inbred (F = 0.17) with relatively low genetic variation (86%), infusion of new founder genes from Asia is a conservation priority. Importation of cryopreserved semen for use with IVF and ET may offer one alternative to the international transport of living animals. In this study, our objectives were to (1) compare motility longevity of fresh vs. frozen-thawed fishing cat spermatozoa in two culture media, (2) evaluate ovarian responses to exogenous gonadotropins, and (3) assess development of IVF embryos produced with fresh vs. frozen-thawed spermatozoa. Raw semen was collected via electroejaculation from male fishing cats (n = 4), divided into groups, and washed. Two sperm pellets were resuspended in either Ham's F10 medium (HF10; with 5% FBS) or our feline optimized culture medium (FOCM; with 0.4% BSA); another pellet was diluted in TEST egg yolk, cooled to 5�C over 3 h, glycerated (4%), and cryopreserved in straws over LN2 vapor. Frozen sperm samples were thawed, washed, and diluted in either HF10 or FOCM. Fresh and frozen-thawed sperm motility (percent motile, rate of forward progress) in each medium (10 � 106 motile sperm/mL) was assessed (at 0, 1, 3, and 6 h) in microdrops under oil during culture (38�C; 6% CO2 in air). Female fishing cats (n = 10) were treated with exogenous gonadotropins (150 IU eCG, 100 IU hCG, 85-h interval) and ovarian follicles were aspirated laparoscopically. Recovered oocytes were inseminated with fresh (2 � 105 motile sperm/mL) or frozen-thawed (5 � 105 motile sperm/mL) spermatozoa in FOCM microdrops; resulting embryos were either cryopreserved or cultured in FOCM (with 5% FBS added at 72 h post-insemination) for 7 days. Sperm motility over time did not differ (P > 0.05) between media for either fresh or frozen-thawed samples; however, across media, frozen-thawed sperm motility was lower (P < 0.05) and declined faster (P < 0.05) compared to fresh spermatozoa. Females produced an average (�SEM) of 9.8 � 2.9 mature ovarian follicles, allowing recovery of 7.3 � 2.6 high-quality oocytes per female. Oocyte cleavage percentage at 42 h p.i. was lower (P < 0.05) with frozen-thawed spermatozoa (38%, 11/29) compared to freshly collected spermatozoa (68%, 17/25). Overall, 35% (6/17) of cultured embryos developed to blastocysts with no difference (P > 0.05) between embryos produced with frozen-thawed (4/11) vs. fresh (2/6) spermatozoa. Although fishing cat sperm motility and fertility appear compromised after cryopreservation, our results demonstrate the ability of frozen-thawed spermatozoa to produce IVF embryos that are capable of developing to blastocyst stage in vitro. This work was supported by (NIH RR015388).

scholarly journals Viabilidad in vitro e in vivo de los espermatozoides congelados/descongelados del conducto deferente de Alpacas (vicugna pacos)

2016 ◽  
Vol 18 (2) ◽  
Author(s):  
Manuel Guido Pérez-Durand ◽  
Juan Pompeyo Zevallos-Aragón ◽  
Uri Harold Pérez-Guerra
Keyword(s):  
Sperm Motility ◽  
Vas Deferens ◽  
Sperm Cells ◽  
Freezing And Thawing ◽  
Sperm Donors ◽  
Similar Percentage ◽  
Vicugna Pacos ◽  
Sperm Freezing

<p>El objetivo del presente estudio fue evaluar la viabilidad <em>in-vitro</em> e <em>in-vivo</em> de los espermatozoides procedente de los conductos deferentes de alpacas. Se utilizaron 2 reproductores como donadores de espermatozoides con desviación del conducto deferente. Los espermatozoides colectados se sometieron a la congelación y descongelación con el dilutor Triladyl®. Durante el procesamiento de los espermatozoides se evaluaron la motilidad y la prueba hipo-osmotica. En la inseminación se evaluó la proporción de gestaciones producidas. Los resultados fueron los siguientes: La motilidad de los espermatozoides para los reproductores 1 y 2 fueron: a los 37°C 65.16 y 63.37% sin diferencia (P&gt;0.05), al enfriamiento (5°C) 52.63 y 48.31% similares (P&gt;0.05) ya la descongelación 24.51 y 33.18% mostraron diferencia (P&lt;0.05). A la prueba hipo-osmótica fueron; a los 37°C 51.55 y 52.98% similares (P&gt;0.05), Al enfriamiento (5°C) 46.67 y 55.93% mostraron diferencia (P&lt;0.05) y a la descongelación 20.06 y 32.18% con diferencia (P&lt;0.05). Las gestaciones fueron: Con espermatozoides frescos diluidos 36.36% (4/11), con espermatozoides descongelados 25.00% (5/20) y con monta 54.54% (6/11), siendo similares (P&gt;0.05). En conclusión los espermatozoides procedentes de los conductos deferentes soportan el congelamiento y a la inseminación artificial producen gestaciones.</p><p><strong>Palabras claves</strong>: Alpaca, conducto deferente, espermatozoide, congelación, inseminación, gestación.</p><p align="center"><strong>ABSTRAC</strong></p><p align="center"><strong>Viability <em>in vitro</em> and <em>invivo</em> of sperm frozen/thawed from Alpaca (<em>vicugna pacos</em>) vas deferens</strong></p><p align="center"><strong><br /></strong></p><p>The aim of this study was to evaluate the <em>in-vitro</em> and <em>in-vivo</em> viability of sperm from the vas deferens alpacas. 2 males as sperm donors were used to offset the vas deferens. The collected sperm cells were subjected to freezing and thawing with dilutor Triladyl®. During processing of sperm motility and hypo-osmotic test they were evaluated. Insemination in the proportion of pregnancies produced was evaluated. The results were as follows: The sperm motility of males 1 and 2 were: at 37 ° C 65.16 and 63.37% with no difference (P&gt; 0.05), cooling (5 ° C) 52.63 and 48.31% similar (P &gt; 0.05) and thawing 24.51 and 33.18% showed no difference (P &lt;0.05). The hypo-osmotic test were; at 37 ° C 51.55 and 52.98 similar percentage (P&gt; 0.05), and cooling 55.93% 46.67 showed no difference (P &lt;0.05) and thawing 20.06 and 32.18% with difference (P &lt;0.05). Pregnancies were diluted with fresh sperm 36.36% (4/11), with defrosted sperm 25.00% (5/20) and 54.54% mounts (6/11), being similar (P&gt; 0.05). In conclusion sperm from the vas deferens withstand freezing and insemination are able to produce pregnancies.</p><p><strong>Keywords</strong>: Alpaca, vas deferens, sperm, freezing, insemination, gestation.</p>

scholarly journals Effect of three commercial extenders on sperm motility and fertility in liquid ram semen stored at 15 °C or 5 °C

2019 ◽  
Vol 67 (3) ◽  
pp. 430-444
Author(s):  
Ander Arando ◽  
Juan Vicente Delgado ◽  
José Manuel León ◽  
Sergio Nogales ◽  
Francisco Javier Navas-González ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Soybean Lecithin ◽  
Egg Yolk ◽  
Kinematic Parameters ◽  
Progressive Motility ◽  
Head Displacement ◽  
Liquid Storage ◽  
Lateral Head

The effect of different extenders on sperm motility and fertility was evaluated during liquid storage of ram semen at 5 °C and 15 °C. The semen was collected, pooled and diluted in three commercial extenders: Inra 96® (INRA) based on skimmed milk, Biladyl® A fraction (BIL) based on egg yolk, and Ovixcell® (OVIX) based on soybean lecithin. Then, sperm motility was evaluated at 0, 6, 24, 48, 72 and 96 h. In order to evaluate fertility, samples stored at 15 °C were used after dilution in INRA and OVIX. Results showed that progressive motility was significantly higher up to 72 h of storage in sperm samples maintained at 5 °C in comparison with 15 °C, similarly for each tested diluent. When samples were stored at 5 °C in OVIX, kinematic parameters such as velocity (except curvilinear velocity, VCL), trajectory [linearity (LIN), straightness (STR), wobble (WOB)], amplitude of lateral head displacement (ALH) and beat/cross frequency (BCF) were higher than in INRA and BIL. No significant differences in pregnancy rate were detected between INRA (62.6%) and OVIX (58.9%). In conclusion, liquid storage at 5 °C with OVIX extender is an interesting option since non-animal components are used, and this extender offers similar in vitro and in vivo efficacy as other extenders containing animal components.

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