Genetic characterization of Markhoz goat breed using microsatellite markers

Abstract. The use of molecular markers can support the management of endangered populations and should be combined with appropriate breeding strategies to improve productive traits avoiding the decline of the breed. The genetic variability at 10 microsatellite loci were investigated in a sample of 100 unrelated Markhoz goats (77 females and 23 males). The investigated population was reared at the Sanandaj Markhoz goat Performance Testing Station in Kurdistan, Iran. Markhoz goat, a multipurpose breed, is one of the most valuable genetic resources in Iran. All the studied loci were found to be polymorphic and a total number of 52 alleles were identified with an average number of alleles of 5.2. Moreover, some population genetic indices, such as observed and expected heterozygosity, observed and expected number of alleles, Shannon's index, Nei's expected heterozygosity, and polymorphism information content were also calculated. Despite the decreasing population size, Markhoz goat genetic diversity is still conserved. The breed seems to have a good level of genetic variability and, as a consequence, a potential margin of adaptability to environment and for future genetic improvement.

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Genetic characterization of the Moxotó goat breed using RAPD markers

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2005000300006 ◽

2005 ◽

Vol 40 (3) ◽

pp. 233-239 ◽

Cited By ~ 12

Author(s):

Rejane Rodrigues de Oliveira ◽

Andréa Alves do Egito ◽

Maria Norma Ribeiro ◽

Samuel Rezende Paiva ◽

Maria do Socorro Maués Albuquerque ◽

...

Keyword(s):

Genetic Variability ◽

Rapd Markers ◽

Genetic Characterization ◽

Random Amplified Polymorphic Dna ◽

Genetic Distances ◽

Goat Breed ◽

Breeding Programs ◽

Analysis Of Molecular Variance ◽

Polymorphic Bands

The objective of this study was to verify the genetic diversity between and within seven populations of Moxotó goat (n = 264) from the States of Pernambuco, Paraíba and Rio Grande do Norte, using RAPD (Random Amplified Polymorphic DNA). Moxotó, as well as other naturalized breeds, suffers genetic losses due to the indiscriminate miscegenation with breeds raised in the Northeast Region of Brazil. The genetic characterization of these genetic resources is essential to conservation and breeding programs. DNA was extracted from lymphocytes using a non-organic protocol. The 16 primers used were selected from 120 decamer oligonucleotide primers and generated 56 polymorphic bands. The analysis of molecular variance (AMOVA) showed that the greater part of total genetic variability (71.55%) was due to differences between individuals within populations, while 21.21% was among populations. The analysis of variance among the pairs of populations demonstrated that the populations located in Floresta, PE x Angicos, RN presented a smaller value of intrapopulational differentiation (8.9%), indicating low genetic variability among them. Nei's genetic distances varied between 0.0546 and 0.1868 in the populations. The dendrogram generated showed that the Canindé breed, used as outgroup, clustered with the populations of Moxotó, indicating a possible common origin of the naturalized goat breeds.

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Genetic Characterization of Cleveland Bay Horse Breed

Diversity ◽

10.3390/d11100174 ◽

2019 ◽

Vol 11 (10) ◽

pp. 174 ◽

Cited By ~ 2

Author(s):

Khanshour ◽

Hempsey ◽

Juras ◽

Cothran

Keyword(s):

Genetic Diversity ◽

Genetic Variability ◽

Genetic Similarity ◽

Genetic Resource ◽

Genetic Characterization ◽

Genetic Relationships ◽

Similarity Measures ◽

Horse Breed ◽

The Usa

The Cleveland Bay (CB) is the United Kingdom’s oldest established horse breed. In this study we analyzed the genetic variability in CB horses and investigated its genetic relationships with other horse breeds. We examined the genetic variability among 90 CB horses sampled in the USA compared to a total of 3447 horses from 59 other breeds. Analysis of the genetic diversity and population structure was carried out using 15 microsatellite loci. We found that genetic diversity in CB horses was less than that for the majority of other tested breeds. The genetic similarity measures showed no direct relationship between the CB and Thoroughbred but suggested the Turkman horses (likely in the lineage of ancestors of the Thoroughbred) as a possible ancestor. Our findings reveal the genetic uniqueness of the CB breed and indicate its need to be preserved as a genetic resource.

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Genetic characterization of local goats of Karnataka by microsatellite marker analysis

Indian Journal of Animal Research ◽

10.18805/ijar.b-3458 ◽

2018 ◽

Author(s):

R. Jayashree ◽

M. R. Jayashankar ◽

C. S. Nagaraja ◽

Isloor Shrikrishna ◽

K. Satyanarayana

Keyword(s):

Microsatellite Marker ◽

Genomic Dna ◽

Genetic Characterization ◽

Expected Heterozygosity ◽

Marker Analysis ◽

Goat Population ◽

Pcr Products ◽

The Mean ◽

Microsatellite Marker Analysis

The diversity status of local goats of Karnataka was studied by using microsatellite marker analysis. The genomic DNA from unrelated local goats were PCR- amplified with a panel of 23 microsatellite markers. Microsatellite PCR products were multiplexed and run on capillary based genetic analyser and the raw data obtained was analysed. Totally 158 alleles were observed and the number of alleles ranged from three (ILSTS005 and OarJMP29) to 13 (RM088). The number of effective alleles ranged from 2.25 (ILSTS005) to 8.40 (RM088) in all the 23 loci studied. The mean observed heterozygosity (Ho) was 0.4698±0.2214 [range 0 (ETH225) to 0.8462 (ILSTS034)] and the mean expected heterozygosity (He) was 0.7471± 0.1098 [range 0.5656 (ILSTS005) to 0.9138 (SRCRSP 8)] indicating the heterogenous nature of the local goat population of Karnataka.

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Genetic characterization of Gaddi goat breed of Western Himalayas using microsatellite markers

Veterinary World ◽

10.14202/vetworld.2015.527-531 ◽

2015 ◽

Vol 8 (4) ◽

pp. 527-531 ◽

Cited By ~ 5

Author(s):

Gurdeep Singh ◽

Yashpal Thakur ◽

Amitoz Kour ◽

Varun Sankhyan ◽

Sanjeet Katoch

Keyword(s):

Microsatellite Markers ◽

Genetic Characterization ◽

Western Himalayas ◽

Goat Breed

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Genetic improvement of farmed tilapia: documentation and genetic characterization of strains

Aquaculture ◽

10.1016/0044-8486(93)90055-4 ◽

1993 ◽

Vol 111 (1-4) ◽

pp. 296 ◽

Cited By ~ 3

Author(s):

J.M. Macranas ◽

A.E. Eknath ◽

L.Q. Agustin ◽

R.R. Velasco ◽

Ma.C.A. Ablan ◽

...

Keyword(s):

Genetic Improvement ◽

Genetic Characterization

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Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines

Ciência Rural ◽

10.1590/0103-8478cr20140132 ◽

2015 ◽

Vol 45 (5) ◽

pp. 877-883 ◽

Cited By ~ 2

Author(s):

Mari Helen Pagani Possamai ◽

Jaqueline Battilana ◽

Ediane Paludo ◽

Marcos Edgar Herkenhoff ◽

Fábio Pértile ◽

...

Keyword(s):

Genetic Variability ◽

Chain Reaction ◽

Blood Samples ◽

Free Range ◽

Allele Number ◽

Expected Heterozygosity ◽

Number Variation ◽

Polymerase Chain ◽

Commercial Chickens

This study aimed to investigate the genetic variability of two Brazilian free range (Caipira) chickens lines using microsatellites analysis of ten loci. It was collected a total of 99 blood samples, which 49 were from Paraíso Pedrês (PP) and 50 were from Rubro Negra (RN) lines. The amplification of the DNA fragments was performed by polymerase chain reaction (PCR) and the genotyping was conduct using ABI 3130 sequencer. The allele number variation was among 3 (LEI0254) to 32 (LEI0212) in the PP line, and 4 (LEI0254) to 31 (LEI0212) in the RN line. The allelic average per locus was 13.3 and 13.1 in the PP and RN lines, respectively. The average observed and the expected heterozygosity were 0.650 and 0.820 in the PP line, and 0.671 and 0.804 in the RN line. All of the analyzed loci were informative (PIC>0.5). These results indicate that these free-range animals have a high genetic variability, at least for the majority of the analyzed loci, and this genetic variation is higher than the commercial chickens and similar for the no-commercial birds

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Genetic Characterization of a Sheep Population in Oaxaca, Mexico: The Chocholteca Creole

Animals ◽

10.3390/ani11041172 ◽

2021 ◽

Vol 11 (4) ◽

pp. 1172

Author(s):

Teodulo Salinas-Rios ◽

Jorge Hernández-Bautista ◽

Araceli Mariscal-Méndez ◽

Magaly Aquino-Cleto ◽

Amparo Martínez-Martínez ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Distance ◽

Genetic Characterization ◽

Polymorphic Information Content ◽

Isolated Population ◽

Effective Number ◽

Sheep Population ◽

Blood Samples ◽

Expected Heterozygosity

Creole sheep in México have undergone crossbreeding, provoking the loss of genetic variability. The objective of the present study is to determine the intra-racial genetic diversity, the genetic relationship with other genotypes, and the populational substructure of the Oaxacan Creole sheep. Twenty-nine blood samples were obtained of Creole sheep of the Oaxaca Mixteca region in México. A genetic analysis was made with 41 microsatellites recommended for studies of genetic diversity in sheep. An analysis was made of genetic diversity, populational structure, and genetic distance with 27 other sheep populations. The study found 205 alleles with a range of 2 to 9 by locus and an effective number of 3.33. The intra-racial analysis showed a moderate genetic diversity with values of expected heterozygosity of 0.686 and observed of 0.756, a mean polymorphic information content of 0.609, and a mean coefficient of consanguinity of −0.002. In interracial genetic diversity for the coefficients of consanguinity, the values were FIS = 0.0774, FIT = 0.16993, and FST = 0.10028, showing an elevated genetic distance with other creole breeds, but close to Argentine Creole, to another Creole of México and the Spanish Merino. Its genetic structure showed that it does not have any populational subdivision nor mixes with the others analyzed. It is concluded that it is a distinct and isolated population and is proposed as the creole breed “Chocholteca” for its conservation.

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Genetic variability in the A microsatellite at SLC11A1 gene and possible implications with innate resistance against brucellosis in Algerian native goats

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d211219 ◽

2020 ◽

Vol 21 (12) ◽

Author(s):

Hossem SAHRAOUI ◽

TOUFIK MADANI ◽

KHALED FANTAZI ◽

ASMA CHAOUCH KHOUANE ◽

ABDELKADER AMEUR AMEUR ◽

...

Keyword(s):

Genetic Variability ◽

Genetic Improvement ◽

Genetic Parameters ◽

Polymorphic Information Content ◽

Genetic Resistance ◽

Agricultural Activities ◽

Improvement Program ◽

Innate Resistance ◽

Slc11a1 Gene ◽

Expected Heterozygosity

Abstract. Sahraoui H, Madani T, Fantazi K, Chaouch Khouane A, Ameur Ameur A, Paschino P, Vacca GM, Gaouar SBS, Dettori ML. 2020. Genetic variability in the A microsatellite at SLC11A1 gene and possible implications with innate resistance against brucellosis in Algerian native goats. Biodiversitas 21: 5630-5636. Goat rearing is among the major agricultural activities practiced in Algeria. However, brucellosis represents an important threat to the goat sector and to public health. The aim of our work was to characterize the genetic variability of the A microsatellite at the 3' untranslated region (3' UTR) of SLC11A1 gene in Algerian native goat breeds as it was associated with Brucellosis genetic resistance in goat. Genomic DNA samples of 90 goats belonging to the four Algerian native breeds; Mekatia (n = 32), Arbia (n = 30), Dwarf of Kabylia (n = 14) and Mozabit (n = 14) have been analyzed. Polymorphism of region A microsatellite was determined by capillary electrophoresis identifying 8 alleles and 20 genotypes. Genetic parameters were also estimated. Polymorphic information content was PIC = 0.60, the observed and the expected heterozygosity were Ho = 0.569 and He = 0.595, respectively, and the values of FIS, FIT and FST were 0.044, 0.064, and 0.021, respectively. The A microsatellite was highly polymorphic and alleles associated genetic resistance against brucellosis in other breeds or species have been identified. These results open a promising opportunity to implement a genetic improvement program to reduce goat brucellosis spread in Algeria.

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Primer Note: A novel set of EST-SSR markers in Tamarix: a resource to characterize this genus

Silvae Genetica ◽

10.1515/sg-2013-0013 ◽

2013 ◽

Vol 62 (1-6) ◽

pp. 104-109 ◽

Cited By ~ 3

Author(s):

S. Terzoli ◽

E. Cattan ◽

M. Sabatti ◽

R. Valentini ◽

A. Zilberstain ◽

...

Keyword(s):

Ssr Markers ◽

Expressed Sequence Tag ◽

Genetic Characterization ◽

Ecological Impact ◽

Expected Heterozygosity ◽

Desert Habitat ◽

The Mean ◽

Expressed Sequence ◽

Simple Sequence

Abstract Both the negative and positive ecological impact of Tamarix plants is controversial, and thus a more comprehensive understanding is necessary. Tamarisks are invasive in many countries but the inter-specific transferability that characterizes simple sequence repeats (SSRs) could be harnessed to track the spread of specific genotypes or to study invasive populations. Thirteen polymorphic SSR markers, derived from expressed sequence tag (EST), were identified by first screening 26 samples of T. aphylla, T. jordanis, T. nilotica, and T. tetragyna and then 33 unidentified tamarisks from Yotvata, Israel. The mean number of alleles per locus ranged from two to 14 and the mean expected heterozygosity was 0.415. These EST-SSR markers will undoubtedly be useful in the genetic characterization of the genus Tamarix due to their high cross-species transferability which enables the estimation of the genetic diversity among and within different species, that are adapted to the same desert habitat under severe environmental constraints.

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Genetic variability of Scots pine (Pinus sylvestris L.) in maternal regions of provenance

Folia Forestalia Polonica ◽

10.1515/ffp-2015-0011 ◽

2015 ◽

Vol 57 (2) ◽

pp. 112-119 ◽

Cited By ~ 1

Author(s):

Paweł Przybylski ◽

Jan Matras ◽

Małgorzata Sułkowska

Keyword(s):

Genetic Variability ◽

Pinus Sylvestris ◽

Scots Pine ◽

Genetic Parameters ◽

Fixation Index ◽

Effective Number ◽

Expected Number ◽

Expected Heterozygosity ◽

Rare Alleles ◽

Fixation Indices

Abstract The analysis of chosen selected Scots Pine (Pinus sylvestris L.), populations representing different seed regions: 107 (Międzyzdroje), 305 (Woziwoda), 206 (Strzałowo), 208 (Białowieża), 504 (Bolesławiec), 606 (Józefów) were performed using 10 isoensyme markers: Gdh (E.C.1.4.1.2), Sdh-A, Sdh-B (E.C.1.1.1.25), Pgd-B (E.C.1.1.1.44), Mdh-A, Mdh-C (E.C.1.1.1.37), Got-A, Got-B, Got-C (E.C.2.6.1.1), Dia-C (E.C.1.8.1.4). There were calculated following genetic parameters: allelic frequencies, observed and expected heterozygosities, and Wright’s fixation indexes. In populations, the results of analysis indicated presence of rare alleles. In all study populations, the average effective number of alleles per locus was 1.46 and was lower than expected number of alleles per locus of 1.93. The results for the effective number of alleles of the population per locus were as follows: Strzałowo and Białowieża 1.54, Bolesławiec 1.48, Jozefów 1.44, Świnoujście 1.42, Woziwoda 1.35. Average observed heterozygosity in the studied populations was calculated at the level of 0.26 and it was lower than the expected heterozygosity at 0.28. For populations, the level of heterozygosities were as follow: Strzałowo 0.35, Bolesławiec and Białowieża 0.25, Józefów 0.27, Świnoujście 0.23, Woziwoda 0.26. In particular loci level of heterozygosity was different, as the most heterozygous Mdh-C locus was estimated, while minimum Got-C. Significant differences in allele frequency of Hardy-Weinberg deviation equilibrium were found in 10 cases: Strzałowo (Got-C), Bolesławiec (Got-B), Białowieża (Got-B, Gdh), Józefów (Mdh-C), Międzyzdroje (Got-B, Got-C, Mdh-C), Woziwoda (Got-C, Gdh). All studied populations had a lower effective number of alleles per locus (Ne) comparing to the observed number of alleles (Na). Wright’s fixation indices were negative for populations: Strzałowo (-0.09), Józefów (-0.03), Międzyzdroje (-0.01) and positive for: Białowieża (0.12), Woziwoda and Bolesławiec (0.07). The extremely high Wright’s fixation index (0.12) was observed for Białowieża population.

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