scholarly journals Monitoring of the Epidemic Situation with Q Fever in the Regions of Ukraine

2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Olha Zarichna
Keyword(s):  
Infectious Diseases ◽  
Coxiella Burnetii ◽  
Causative Agent ◽  
Q Fever ◽  
Epidemiological Surveillance ◽  
Epidemic Process ◽  
Zoonotic Infections ◽  
Natural Foci ◽  
Serological Studies ◽  
Endemic Areas

ObjectiveTo investigate Q fever pathogen distribution among ixodic ticks, myomorphic rodents, febrile patients, residents of enzootic areas with Q fever and persons in contact with Q fever, specifically infected persons in the Southern and Western regions of Ukraine.IntroductionImprovement of the Q fever epizootic and epidemiological surveillance system remains an urgent veterinary service and healthcare problem in Ukraine. The grounds for this should be laid by the results of monitoring studies of persons with a professional infection risk (livestock farms, animal processing enterprises, veterinary specialists, etc.) and living in enzootic territories , as well as research of Q fever pathogen possible sources reservoirs.MethodsReal-time PCR - detection of specific DNA segments of Coxiella burnetii with application of commercial reagent kits. Immunofluorescence microscopy - detection of antigens/antibodies of studied rickettsia in biological substrates using luminescent immune sera labeled with fluorescein-5-isothiocyanate. Epidemiological methods - analysis of infectious diseases foci epidemiological maps. Statistical methods - data analysis using such software as Excel and Quantum GIS (1.6.0).ResultsPrimarily, Q fever endemic areas are formed because of the circulation of Coxiella burnetii in warm-blooded animal populations and their blood-sucking ectoparasites, which are the main source of the infection in humans. Based on the aggregated data received from multi-year research projects in Ukraine, Q fever enzootic territories were found in 18 administrative regions, Crimea and the city of Sevastopol. Currently we know of 257 areas where the pathogen was detected. The epidemic process in these territories is manifested by sporadic human diseases and the detection of the pathogen in natural carriers. The possibility of the natural foci epidemic potential increase in these territories is confirmed by the higher titers of Q fever pathogen specific antibodies detected in the local population.The results of the research of the infected material that was collected in Southern Ukraine during 2014-2016, showed the preservation of the Q fever causative agent in natural foci both in Danube-Dniester interfluve area of Odesa region and in Trans-Dnistrer areas, and its significantly less prevalent in the area adjacent to Odessa. In addition, the signs of natural foci formation have been revealed in other areas, which is indicative of current epidemic activity of natural foci of the infection. The results of serological studies and clinical and epidemiological surveys indicate that in the immunological structure of the population of the Danube-Dniester interfluve areas of Odessa region, Q fever is most common in rural population of working age, especially those constantly contact with farm animals. In the Ivano-Frankivsk region, serological studies in 2014 -2016, detected no Q fever seropositive people, indicating the pathogen being in the reserve stage, which corresponds to the inter-epidemic period. At the same time, the detection of C. burnetii in ticks in the enzootic territories indicates the possibility of the pre-epidemic process being formed.Since by pathogen range and transmission mechanisms Q fever in Ukraine is associated with many natural-focal zoonotic infections, it is advisable to monitor endemic areas using a modern observation algorithm using the introduction of geoinformation systems and the molecular genetic characteristics of circulating strains. This will increase the effectiveness of the detection of current natural and anthropurgic foci of such infections, will contribute to their detailed characterization and systematization, improve epidemiological surveillance and prevent the emergence of epidemic outbreaks among the population. The results of the research will contribute to the improvement of differential diagnosis of febrile states with an unclear etiologic agent.ConclusionsThe results of the Q fever pathogen detection in the material collected in Southern and Western regions of Ukraine showed that the area of prevalence of this agent has been expanded to the areas and settlements that are not included in the list of enzootic territories. Involvement in the ecological cycles of ixodic ticks and mouse-like rodents was observed. The presence of polyvectoral and polyhostal natural foci of this infection was found. The circulation of the causative agent of Q fever in the territories of Odesa and Ivano-Frankivsk regions where epidemic outbreaks and sporadic disease in people were also observed.References1. Surveillance Atlas of Infectious Diseases // http://atlas.ecdc.europa.eu/public/index.aspx.2. UCDCM Information Sheet as of 07/21/2010 No. 04.4-31/40/868 On Epidemic and Epizootic Situation with Zoonotic Infections Common for Humans and Animals and Methods of their Prevention in Ukraine.

scholarly journals Coxiella burnetii, the causative agent of Q fever in Saudi Arabia: molecular detection from camel and other domestic livestock

2014 ◽  
Vol 7 (9) ◽  
pp. 715-719 ◽  
Author(s):  
Osama B. Mohammed ◽  
Abdulrahman A. Jarelnabi ◽  
Riyadh S. Aljumaah ◽  
Mohammed A. Alshaikh ◽  
Amel O. Bakhiet ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Coxiella Burnetii ◽  
Causative Agent ◽  
Molecular Detection ◽  
Q Fever ◽  
Domestic Livestock

scholarly journals PROBLEMS IN THE ORGANIZATION OF EPIDEMIC SURVEILLANCE OF LEPTOSPIROSIS IN A SUBJECT OF THE RUSSIAN FEDERATION

2012 ◽  
Vol 17 (1) ◽  
pp. 46-48
Author(s):  
A. A. Nafeyev
Keyword(s):  
Infectious Diseases ◽  
Russian Federation ◽  
Climatic Factors ◽  
Laboratory Diagnosis ◽  
Natural Focus ◽  
Epidemic Process ◽  
Natural Foci ◽  
The Russian Federation ◽  
Active Natural

This paper characterizes the epidemic process of leptospirosis. The current new herd infections are typified by: 1) the formation of new natural foci under anthropogenic and climatic factors; 2) the change in the biocenotic structure of natural foci; 3) the formation of natural foci of mixed infectious diseases of bacterial and virus etiology; 4) the change in the epidemic potential of natural foci; 5) synathropization of infectious diseases. Few or no registered leptospirosis cases in some silent areas are frequently attributable to poor differential, including laboratory, diagnosis. The epidemic process is characterized in terms of the active natural focus of leptospirosis in a long-term period.

scholarly journals C. burnetii Shedding Study In Domestic Animals in Georgia

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Marine Nikolaishvili
Keyword(s):  
Coxiella Burnetii ◽  
Milk Sample ◽  
Q Fever ◽  
Small Ruminants ◽  
Domestic Animals ◽  
Epidemiological Surveillance ◽  
Bacterial Disease ◽  
Animal Blood ◽  
The Third ◽  
Seropositive Animal

ObjectiveQ fever is poorly understood in Georgia and its prevalence is largely underestimated in both humans and animals.One of the main goal of the project was shedding study in domestic animals – isolation of C. burnetii from suspected seropositive animal blood, milk samples.IntroductionQ fever is a zoonotic bacterial disease resulting from infection by Coxiella burnetii. Domestic ruminants (cattle, sheep, and goats) are considered the main reservoir for the pathogen, which can also infect humans. Q fever is poorly understood in Georgia and its prevalence is largely underestimated in both humans and animals.In Georgia Q fever laboratory diagnostic was started and implemented at the Laboratory of the Ministry of Georgia (LMA) within GG20 ,,Prevalence, Epidemiological Surveillance, and Laboratory Analysis of Coxiella burnetii in Georgia’’.MethodsLMA conducted Coxiella burnetii shedding evaluation in three specific farms from Kvemo Kartli (Tsalka, Dmanisi) and Mtskheta-Mtianeti (Dusheti). Seropositive cattle and small ruminants were sampled per week. Sampling lasted 7 weeks and totally 581 samples samples (blood, milk and swab) were tested. Testing were conducted in a BSL3 laboratory under BSL3 working conditions. ACCM medium was used (2XACCm-2 acidified Citrate Cysteine Medium PH-4.75G N NaOH). The samples were incubated at 37°C using CO2.ResultsAs a result of the study, one culture was bacteriologically isolated from seropositive cattle milk sample ( the sample was taken on the third week of the study in Beshtasheni farm, Tslka, Kvemo Kartli) and confirmed by Molecular biology (PCR).ConclusionsThe study confirmed Q fever existence in Georgia. Traditionally considered an obligate intracellular agent, the requirement to be grown in tissue culture cells, embryonated eggs, or animal hosts has made it difficult to isolate C. Burnetii strains. Within the study one culture was isolated from the seropositive animal milk sample that was collected in the third week of the study. shedding of Coxiella burnetii in milk by infected cows appeared to be the most frequent positive sample for the bacterium. 

scholarly journals Serological evidence of single and mixed infections of Rift Valley fever virus, Brucella spp. and Coxiella burnetii in dromedary camels in Kenya

2021 ◽  
Vol 15 (3) ◽  
pp. e0009275
Author(s):  
Mathew Muturi ◽  
James Akoko ◽  
Daniel Nthiwa ◽  
Bernard Chege ◽  
Richard Nyamota ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Q Fever ◽  
Rift Valley Fever Virus ◽  
Strong Association ◽  
Zoonotic Diseases ◽  
Fever Virus ◽  
Valley Fever ◽  
Zoonotic Infections

Camels are increasingly becoming the livestock of choice for pastoralists reeling from effects of climate change in semi-arid and arid parts of Kenya. As the population of camels rises, better understanding of their role in the epidemiology of zoonotic diseases in Kenya is a public health priority. Rift Valley fever (RVF), brucellosis and Q fever are three of the top priority diseases in the country but the involvement of camels in the transmission dynamics of these diseases is poorly understood. We analyzed 120 camel serum samples from northern Kenya to establish seropositivity rates of the three pathogens and to characterize the infecting Brucella species using molecular assays. We found seropositivity of 24.2% (95% confidence interval [CI]: 16.5–31.8%) for Brucella, 20.8% (95% CI: 13.6–28.1%) and 14.2% (95% CI: 7.9–20.4%) for Coxiella burnetii and Rift valley fever virus respectively. We found 27.5% (95% CI: 19.5–35.5%) of the animals were seropositive for at least one pathogen and 13.3% (95% CI: 7.2–19.4%) were seropositive for at least two pathogens. B. melitensis was the only Brucella spp. detected. The high sero-positivity rates are indicative of the endemicity of these pathogens among camel populations and the possible role the species has in the epidemiology of zoonotic diseases. Considering the strong association between human infection and contact with livestock for most zoonotic infections in Kenya, there is immediate need to conduct further research to determine the role of camels in transmission of these zoonoses to other livestock species and humans. This information will be useful for designing more effective surveillance systems and intervention measures.

scholarly journals DNA microarray-based detection of Coxiella burnetii, the causative agent of Q fever

2014 ◽  
Vol 56 (1) ◽  
pp. 27 ◽  
Author(s):  
Gernot Schmoock ◽  
Ralf Ehricht ◽  
Lisa D Sprague
Keyword(s):  
Dna Microarray ◽  
Coxiella Burnetii ◽  
Causative Agent ◽  
Q Fever

scholarly journals A CASE OF Q FEVER IN NON-ENDEMIC AREAS

2012 ◽  
Vol 17 (6) ◽  
pp. 38-40
Author(s):  
A. A. Nafeev ◽  
V. V. Bezik
Keyword(s):  
Coxiella Burnetii ◽  
Q Fever ◽  
Geographical Area ◽  
Clinical Manifestations ◽  
Epidemiological Data ◽  
Definite Diagnosis ◽  
Serological Tests ◽  
Acute Case ◽  
The Subject ◽  
Endemic Areas

Coxiellosis (Q-fever) is a systemic natural focal zooanthroponosis disease, which has a very wide geographical area. A variety of clinical manifestations and the absence of pathognomonic signs of illness forces to use for making a definite diagnosis complex of techniques including epizootological, epidemiological data, clinical manifestations, and certainly the results of several serological tests designed to detect antibodies to Coxiella burnetii. There is presented a description of an acute case of Q - fever in a child in the subject, characterized as trouble-free of Coxiellosis from 70-ies of the last century.

Current Status of the Natural Foci of Tick-Borne Diseases in the Stavropol Region

2021 ◽  
pp. 72-78
Author(s):  
NF Vasilenko ◽  
DA Prislegina ◽  
EA Manin ◽  
LI Shaposhnikova ◽  
UM Ashibokov ◽  
...  
Keyword(s):  
Q Fever ◽  
Hemorrhagic Fever ◽  
Mineral Waters ◽  
Epidemiological Surveillance ◽  
Current Status ◽  
Crimean Congo Hemorrhagic Fever ◽  
Tick Abundance ◽  
Wide Range ◽  
Natural Foci ◽  
Stavropol Region

Background: Tick-borne diseases are a serious threat to the epidemiological safety of the population of the Stavropol Region, making up more than 70 per cent of all natural focal infectious diseases registered in this territory annually. A wide range of hosts contributes to high tick abundance, diversity of tick-borne pathogens, and maintenance of the natural foci. Objective: To assess the activity of natural foci of tick-borne infections in the Stavropol Region in 2016–2020. Materials and methods: We used notifications submitted by the Center for Hygiene and Epidemiology in the Stavropol Region to the Scientific and Methodological Center for Monitoring the Pathogens of Infectious and Parasitic Diseases of Pathogenicity Groups II–IV for the subjects of the North Caucasian and Southern Federal Districts and the results of an epizootological survey of the Stavropol territory by specialists of the Stavropol Plague Control Research Institute. Markers of the causative agents of tick-borne diseases were detected by ELISA and PCR methods. Data processing was carried out using Microsoft Excel 2010. Results: Active natural foci of Crimean-Congo hemorrhagic fever, Q fever, a group of tick-borne spotted fevers, Lyme disease, human granulocytic anaplasmosis, and human monocytic ehrlichiosis were established in the region in 2016–2020. Human cases of the Crimean-Congo hemorrhagic fever, Lyme borreliosis and Q fever were also registered annually. The abundance of infected ticks in resort towns of the Caucasian Mineral Waters is of particular concern. Conclusion: We established up-to-date epizootic and epidemic manifestations of tick-borne infections in the Stavropol Region in the modern period, which are an important link in epidemiological surveillance and the basis for improving preventive measures

Loss of TSS1 in hypervirulent Coxiella burnetii 175, the causative agent of Q fever in French Guiana

2015 ◽  
Vol 41 ◽  
pp. 35-41 ◽  
Author(s):  
Felicetta D’Amato ◽  
Carole Eldin ◽  
Kalliopi Georgiades ◽  
Sophie Edouard ◽  
Jeremy Delerce ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Causative Agent ◽  
French Guiana ◽  
Q Fever

Proteomic comparison of virulent phase I and avirulent phase II of Coxiella burnetii, the causative agent of Q fever

2011 ◽  
Vol 74 (10) ◽  
pp. 1974-1984 ◽  
Author(s):  
Ludovit Skultety ◽  
Martin Hajduch ◽  
Gabriela Flores-Ramirez ◽  
Ján A. Miernyk ◽  
Fedor Ciampor ◽  
...  
Keyword(s):  
Phase I ◽  
Phase Ii ◽  
Coxiella Burnetii ◽  
Causative Agent ◽  
Q Fever

scholarly journals Problems of Epidemiological Surveillance of West Nile Fever in Ukraine

2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Iryna Demchyshyna ◽  
Yuryi Novohatniy ◽  
Igor Nebogatkin
Keyword(s):  
Causative Agent ◽  
Migratory Birds ◽  
State Institution ◽  
Steppe Zone ◽  
Epidemiological Surveillance ◽  
Laboratory Diagnostics ◽  
West Nile Fever ◽  
West Nile ◽  
Ministry Of Health ◽  
Natural Foci

ObjectiveTo define the problems of epidemiological surveillance of West Nile fever (WNF) in Ukraine.IntroductionFlaviviridae are one of the most widespread arboviruses in Ukraine. Mosquitoes are vectors of WNF in a majority of cases due to bites during swimming, fishing, work in suburban areas and outdoor recreation without use of individual protection from mosquitoes.A study of the species composition of bloodsucking mosquitoes is conducted in Ukraine. Existence of natural foci of WNF viruses has been well-proven all over the territory of Ukraine by testing IgG antibodies in different groups of population, including children [1]. Also, infection of mosquitoes (RNA found in Culex pipiens (including Culex pipiens f. molestus, Culiseta annulata)) was registered. Infection of I. ricinus and D. reticulates was also determined, and it acts as a factor for circulation of virus in the wild too [2].MethodsStatistical, serological and epidemiological methods were used during the study. Serological tests included reactions with IgM and IgG antibody in human serum performed using immunofluorescent and ELISA methods.ResultsIn Ukraine, the causative agent of WNF is detected in all landscapes. It is the main arboviral infection in the forest-steppe zone (53.1 % among all arboviral infections). Enzootic territories are located in 18 regions, 47 administrative districts, and 63 settlements.The majority of natural foci of WNF is located in the Dnieper left-bank steppes, and also in North-Western and Western forest-steppes. The enzootic territories are located on the East of steppe zone and on the East of forest-steppes. The smallest number of natural foci is registered in the Dnieper right-bank part of the steppes. Enzootic territories are absent in Chernivtsi, Chernihiv, Sumy, Ternopil, Luhansk, Kirovohrad Oblasts and Kyiv. Most of them are located in Zaporizhzhia with 9 administrative districts and 16 settlements; in Rivno Oblast - 7 and 9; in Kherson - 5 and 4, and in Poltava Oblasts - 2 and 4 respectively [3].During the period from 2007 to 2016, 86 cases of WNF were registered. WNF was registered in 7 oblasts (Zaporizhzhya - 40 cases, Poltava - 24, Donetsk - 16, Mykolaiv- 3, Kherson, Kharkiv, Zhytomyr Oblasts - one case in each) [4].Registration of WNF cases separately from other viral hemorrhagic fevers has been conducted in the country since 2010 (official registration of total amount of viral hemorrhagic fevers has been performed since 2005).In enzootic territories, 2 cases of the diseases were registered and were associated with ticks bites. The strains of WNV were detected in bloodsucking mosquitoes in Rivne and Zaporizhzhia Oblasts and in tick samples of Ixodes genus collected in Lviv Oblast (probably may be found in other species of tick (Argasidae and Gamazoidea) where the causative agent is kept in natural foci under unfavorable conditions).Laboratory diagnostics was conducted (mainly retrospectively) in Zaporizhzhia, Poltava, Donetsk Oblastss. All diagnoses (exception Mykolaiv Oblast in 2011, data is absent) were laboratory confirmed, including 10 cases confirmed in the State Institution Lviv Research Institute of Epidemiology and Hygiene of the Ministry of Health of Ukraine, and 3 more cases were confirmed by a private laboratory [2].In total, 129 samples of blood sera collected from patients with clinical manifestations of a fever of unknown origin were delivered to the Laboratory of Virology of Ukrainian Center for Diseases Control and Monitoring during 2016-2017. Samples were investigated using the immunofluorescent and enzyme immunoassay methods including immunoblot. West Nile virus markers such as IgM/IgG antibodies have been detected in 4 cases (Poltava oblast) [4].ConclusionsMainly, single cases were registered. It is caused by insufficient level of diagnostics in most of the regions, as a result, diseases pass under other diagnoses. Migratory birds (3 flyways of migratory birds pass through Ukraine) and local animals (crows, jackdaws, doves and other) may be the possible reservoirs of causative agent of WNF. Laboratory diagnostics need to be improved and more attention should be paid to testing of samples of blood serum from patients with suspected WNF.References[1] Rusev I.T., Zakusilo V.M., Vinnuk V.D. Bloodsucking mosquitoes of urbanized biocenosis and their role are in circulation of viruses of West Nile fever. Series are "Biology, chemistry". issue 24 (63). 2011. No. 2. p. 240-248.[2] Lozinskyi I.M., Beletska G.V., Drul O.S., Fedoruck V.I., Kozlovskyi M.M., Rogochiy E.G., Sholomey M.V., Ben I.I., Shulgan A.M./Epidemic situation of Western Nile fever in Ukraine. Magazine of infectology, issue 6, No. 2, 2014 Appendix 66-65.[3] Official data of state statistic form of the Ministry of Health.[4] Data of the State Institution Ukrainian center for Diseases Control and Monitoring of the Ministry of Health of Ukraine.

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