Influence of Spermatozoa Quality on Rate of Oocyte Fertilization under the in Vitro Conditions

2021 ◽  
pp. 62-76
Author(s):  
Vladimir Aleksandrovich Pleshkov ◽  
Oksana Vladimirovna Smolovskaya ◽  
Tat`yana Vladimirovna Zubova ◽  
Anna Vyacheslavovna Semechkova ◽  
Roman Olegovich Kas`yanov
Keyword(s):  
Oocyte Fertilization

scholarly journals Sperm modulate uterine immune parameters relevant to embryo implantation and reproductive success in mice

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
John E. Schjenken ◽  
David J. Sharkey ◽  
Ella S. Green ◽  
Hon Yeung Chan ◽  
Ricky A. Matias ◽  
...  
Keyword(s):  
Reproductive Success ◽  
Seminal Fluid ◽  
Embryo Implantation ◽  
Reproductive Investment ◽  
Global Gene Expression ◽  
Wild Type ◽  
Mouse Uterus ◽  
Immune Parameters ◽  
Oocyte Fertilization

AbstractSeminal fluid factors modulate the female immune response at conception to facilitate embryo implantation and reproductive success. Whether sperm affect this response has not been clear. We evaluated global gene expression by microarray in the mouse uterus after mating with intact or vasectomized males. Intact males induced greater changes in gene transcription, prominently affecting pro-inflammatory cytokine and immune regulatory genes, with TLR4 signaling identified as a top-ranked upstream driver. Recruitment of neutrophils and expansion of peripheral regulatory T cells were elevated by seminal fluid of intact males. In vitro, epididymal sperm induced IL6, CXCL2, and CSF3 in uterine epithelial cells of wild-type, but not Tlr4 null females. Collectively these experiments show that sperm assist in promoting female immune tolerance by eliciting uterine cytokine expression through TLR4-dependent signaling. The findings indicate a biological role for sperm beyond oocyte fertilization, in modulating immune mechanisms involved in female control of reproductive investment.

scholarly journals Oviductal response to gametes and early embryos in mammals

Reproduction ◽  
2016 ◽  
Vol 152 (4) ◽  
pp. R127-R141 ◽  
Author(s):  
Veronica Maillo ◽  
Maria Jesus Sánchez-Calabuig ◽  
Ricaurte Lopera-Vasquez ◽  
Meriem Hamdi ◽  
Alfonso Gutierrez-Adan ◽  
...  
Keyword(s):  
Embryo Development ◽  
Reproductive Tract ◽  
Early Embryo ◽  
Secretory Cells ◽  
Oocyte Fertilization ◽  
Early Embryos ◽  
Significant Research ◽  
Oviductal Fluid

The oviduct is a complex and organized thin tubular structure connecting the ovary with the uterus. It is the site of final sperm capacitation, oocyte fertilization and, in most species, the first 3–4days of early embryo development. The oviductal epithelium is made up of ciliary and secretory cells responsible for the secretion of proteins and other factors which contribute to the formation of the oviductal fluid. Despite significant research, most of the pathways and oviductal factors implicated in the crosstalk between gametes/early embryo and the oviduct remain unknown. Therefore, studying the oviductal environment is crucial to improve our understanding of the regulatory mechanisms controlling fertilization and embryo development. In vitro systems are a valuable tool to study in vivo pathways and mechanisms, particularly those in the oviducts which in livestock species are challenging to access. In studies of gamete and embryo interaction with the reproductive tract, oviductal epithelial cells, oviductal fluid and microvesicles co-cultured with gametes/embryos represent the most appropriate in vitro models to mimic the physiological conditions in vivo.

scholarly journals Follicular Proinflammatory Cytokines and Chemokines as Markers of IVF Success

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Aili Sarapik ◽  
Agne Velthut ◽  
Kadri Haller-Kikkatalo ◽  
Gilbert C. Faure ◽  
Marie-Christine Béné ◽  
...  
Keyword(s):  
Polycystic Ovary ◽  
Unexplained Infertility ◽  
Ovary Syndrome ◽  
Cytokines And Chemokines ◽  
Tubal Factor Infertility ◽  
Oocyte Fertilization ◽  
Tubal Factor ◽  
Mrna Analysis ◽  
Bender Medsystems

Cytokines are key modulators of the immune system and also contribute to regulation of the ovarian cycle. In this study, Bender MedSystems FlowCytomix technology was used to analyze follicular cytokines (proinflammatory: IL-1β, IL-6, IL-18, IFN-γ, IFN-α, TNF-α, IL-12, and IL-23;, and anti-inflammatory: G-CSF), chemokines (MIP-1α, MIP-1β, MCP-1, RANTES, and IL-8), and other biomarkers (sAPO-1/Fas, CD44(v6)) in 153 women undergoingin vitrofertilization (IVF). Cytokine origin was studied by mRNA analysis of granulosa cells. Higher follicular MIP-1αand CD44(v6) were found to correlate with polycystic ovary syndrome, IL-23, INF-γ, and TNF-αwith endometriosis, higher CD44(v6) but lower IL-βand INF-αcorrelated with tubal factor infertility, and lower levels of IL-18 and CD44(v6) characterized unexplained infertility. IL-12 positively correlated with oocyte fertilization and embryo development, while increased IL-18, IL-8, and MIP-1βwere associated with successful IVF-induced pregnancy.

Transferability and inter-laboratory variability assessment of the in vitro bovine oocyte fertilization test

2015 ◽  
Vol 51 ◽  
pp. 106-113 ◽  
Author(s):  
Irene Tessaro ◽  
Silvia C. Modina ◽  
Gabriella Crotti ◽  
Federica Franciosi ◽  
Silvia Colleoni ◽  
...  
Keyword(s):  
Bovine Oocyte ◽  
Oocyte Fertilization

Oocyte fertilization in vitro is associated with high follicular immunoreactive ß-endorphin levels

1989 ◽  
Vol 12 (10) ◽  
pp. 693-698 ◽  
Author(s):  
F. Facchinetti ◽  
P. G. Artini ◽  
M. Monaco ◽  
A. Volpe ◽  
A. R. Genazzani
Keyword(s):  
Fertilization In Vitro ◽  
Oocyte Fertilization
Sign in / Sign up

Export Citation Format

Share Document