scholarly journals Effect of Smear Layer on Penetration Depth of Methylene Blue and Curcumin into Root Dentinal Tubules

2021 ◽  
Vol 6 (4) ◽  
pp. 8-13
Author(s):  
R Ghaseminejad ◽  
A Azizi ◽  
M Fazlyab ◽  
S Toursavadkouhi ◽  
◽  
...  
Keyword(s):  
Methylene Blue ◽  
Penetration Depth ◽  
Smear Layer ◽  
Dentinal Tubules

scholarly journals Debunking the Concept of Dentinal Tubule Penetration of Endodontic Sealers: Sealer Staining with Rhodamine B Fluorescent Dye Is an Inadequate Method

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3211
Author(s):  
David Donnermeyer ◽  
Sina Schmidt ◽  
Arno Rohrbach ◽  
Johannes Berlandi ◽  
Sebastian Bürklein ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Rhodamine B ◽  
Root Canal ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
Ah Plus ◽  
Root Dentin ◽  
Rhodamine B Dye

The aim of this study was to investigate the suitability of rhodamine B dye staining of an epoxy resin sealer (AH Plus) and calcium-silicate-based sealers (Total Fill BC Sealer, BioRoot RCS) to represent the penetration depth of the sealers into dentinal tubules after root canal obturation. In a three-step process, (1) leaching of rhodamine B from sealers into a buffer solution, (2) passive penetration of leached rhodamine B into dentinal tubules, and (3) conformity of rhodamine B penetration assessed by confocal laser scanning microscopy (CLSM), and sealer penetration assessed by scanning electron microscopy (SEM), in root-canal-filled teeth, were evaluated. Rhodamine B dye massively leached out of Total Fill BC Sealer and BioRoot RCS into the phosphate-buffered saline (PBS). A pinkish coloration of AH Plus was found after contact with PBS. Leached rhodamine B dye passively penetrated dentinal tubules from all three sealers when placed on root dentin. No correlation was observed between sealer penetration in SEM and rhodamine B penetration in CLSM. Staining of sealers using rhodamine B is an inadequate method with which to evaluate sealer penetration depth into dentinal tubules, as it overestimates the penetration of sealers into root dentin tubules.

scholarly journals Development of Rotational Intraperitoneal Pressurized Aerosol Chemotherapy to Enhance Drug Delivery into the Peritoneum

2021 ◽  
Author(s):  
Soo Jin Park ◽  
Eun Ji Lee ◽  
Hee Su Lee ◽  
Junsik Kim ◽  
Sunwoo Park ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Methylene Blue ◽  
Penetration Depth ◽  
Flow Rate ◽  
Tissue Concentration ◽  
Palliative Therapy ◽  
Drug Distribution ◽  
Pharmacokinetic Properties ◽  
Blue Solution ◽  
Restricted Use

Abstract Background Pressurized intraperitoneal aerosol chemotherapy (PIPAC) has been introduced as palliative therapy for treating peritoneal metastasis (PM) of solid tumors. However, restricted use in the limited countries and the uneven distribution and penetration in various regions of the peritoneal cavity ac as disadvantages of PIPAC. Thus, the KOrean Rotational Intraperitoneal pressurized Aerosol chemotherapy (KORIA) trial group developed rotational intraperitoneal pressurized aerosol chemotherapy (RIPAC) for enhancing drug delivery into the peritoneum to treat PM, and evaluated the drug distribution, tissue concentrations, penetration depth, pharmacokinetic properties, and toxicities after RIPAC with doxorubicin in pigs. Methods For delivering doxorubicin as aerosols, we used our prototype for PIPAC, which sprayed about 30-µm sized droplets through the nozzle. The mean diameter of the sprayed region was 18.5 cm, and the penetration depth ranged from 360 to 520 µm, comparable to the microinjection pump (Capnopen®; Capnomed, Villingendorf, Germany). For RIPAC, a conical pendulum motion device was added to PIPAC for rotating the nozzle. RIPAC and PIPAC were conducted using 150 ml of 1% methylene blue to evaluate drug distribution and 3.5 mg of doxorubicin in 50 ml of 0.9% NaCl to evaluate tissue concentration and penetration depth, pharmacokinetic properties, and toxicities. All agents were sprayed as aerosols via the nozzle with a velocity of 5 km/h at a flow rate of 30 ml/min under a pressure of 7 bars, and capnoperitoneum of 12 mmHg was maintained for 30 minutes. As a control, we conducted early postoperative intraperitoneal chemotherapy (EPIC) using 1% methylene blue solution with an infusion flow rate of 100 ml/min for 30 minutes and the drainage of 1 L every 10 minutes. Results RIPAC showed a wider distribution and stronger intensity than EPIC and PIPAC. Moreover, the tissue concentration and penetration depth of doxorubicin were higher in RIPAC than in PIPAC. In RIPAC, the pharmacokinetic properties reflected hemodynamic changes during capnoperitoneum, and there were no renal and hepatic toxicities related to RIPAC using doxorubicin. Conclusions RIPAC may have the potential to enhance drug delivery into the peritoneum compared to PIPAC.

Comparative evaluation of sealer penetration depth into radicular dentinal tubules using confocal scanning microscope: an in vitrostudy

2020 ◽  
Vol 02 (03) ◽  
pp. 69-74
Author(s):  
Ambika Sigadam ◽  
Kalyan Satish R ◽  
Girija S Sajjan ◽  
Madhu Varma K ◽  
Sita Ram Kumar M ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Comparative Evaluation ◽  
Scanning Microscope ◽  
Dentinal Tubules ◽  
Confocal Scanning

Evaluation of Residual Dentin after Conventional and Chemomechanical Caries Removal Using SEM

2007 ◽  
Vol 32 (2) ◽  
pp. 115-120 ◽  
Author(s):  
Fernanda Nahás Pires Corrêa ◽  
Leonardo Eloy Rodrigues Filho ◽  
Célia Regina Martins Delgado Rodrigues
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
In Vitro Study ◽  
Adhesive System ◽  
Smear Layer ◽  
Dentinal Tubules ◽  
Removal Method ◽  
Caries Removal ◽  
Scanning Electron

The purpose of this in vitro study was to analyze the residual dentinal surfaces following caries removal using rotatory instruments and two chemomechanical methods (Papacárie® and Carisolv®), by scanning electron microscopy (SEM). Thirty primary incisors were divided into three groups, according to the caries removal method used, and their residual dentin was examined under SEM (15). After caries removal, 15 of these teeth were restored with Single Bond (3M) adhesive system and Z100 Filtek composite resin (3M). The tags of the replicas were observed under SEM. The chemomechanical caries removal methods (Papacárie®and Carisolv®) formed an amorphous layer, similar to the smear layer and few exposed dentinal tubules;the conventional caries removal method produced a smooth and regular dentinal surface, with typical smear layer and exposed dentinal tubules. All groups showed abundant tag formation. Scanning electron microscopy analysis revealed a difference between dentin treated with rotatory instruments and that treated with chemomechanical methods in spite of the occurrence of a similar tag formation in both groups.

Effect of Smear Layer and Direction of Dentinal Tubules on Osteoblast Adhesion to Human Dentin Tissue

2000 ◽  
Vol 26 (6) ◽  
pp. 318-320 ◽  
Author(s):  
Q ZHU ◽  
R HAGLUND ◽  
J CHIOU ◽  
J DEANIII
Keyword(s):  
Smear Layer ◽  
Dentinal Tubules ◽  
Human Dentin ◽  
Osteoblast Adhesion

scholarly journals Ultrastructural analysis of uninstrumented root canal areas following various irrigation regimens

2016 ◽  
Vol 63 (2) ◽  
pp. 57-65 ◽  
Author(s):  
Radomir Barac ◽  
Jelena Popović ◽  
Slavoljub Živković ◽  
Aleksandar Mitić ◽  
Marija Nikolić
Keyword(s):  
Root Canal ◽  
Control Sample ◽  
Physiological Saline ◽  
Endodontic Treatment ◽  
Smear Layer ◽  
Favorable Effect ◽  
Canal Wall ◽  
Saline Irrigation ◽  
Dentinal Tubules ◽  
Left Behind

Abstract Introduction During endodontic treatment smaller or larger areas of root canal wall remain non-instrumented. This can affect prognosis of endodontic treatment as some bacteria may be left behind. The purpose of this study was to evaluate the morphology of non-instrumented areas of the root canal wall using scanning-electron-microscopy (SEM) after completed instrumentation and various irrigation regiments. Materials and Methods Eighteen single-rooted extracted teeth were divided into the six groups. One tooth in each group represented a control sample. In all samples only one half of the canal was instrumented using ISO 40 hand files. Control samples were subjected to an irrigation protocols without instrumentation. Irrigants used were physiological saline, 3% sodium hypochlorite and 15% of ethylene-diamine-tetra-acetate. Irrigation protocol included using each of these irrigants alone, or a combination of NaOCl and EDTA, as well as their combination with final irrigation using NaOCl or chlorhexidine. Then after, roots were sectioned longitudinally and prepared for SEM. Results Saline irrigation left pulpal debris on uninstrumented areas of the canal wall. Irrigation with 3% NaOCl left behind canal wall with different forms of calcospherites. However, after EDTA irrigation dentin appeared as an undulating surface with open tubules without a smear layer. The combination of NaOCl and EDTA showed remnants of calcospherites and open slightly widened dentinal tubules. Final irrigation with NaOCl on the uninstrumented areas showed enlarged dentinal tubules along with dentinal erosion, while after final irrigation with CHX clean dentin and open dentinal tubules without smear layer were noted. Conclusion From the morphological point of view, the most favorable effect of irrigation on both uninstrumented and uninstrumented canal walls was achieved after irrigation with NaOCl and EDTA or NaOCl, EDTA and chlorhexidine as the final irrigant.

scholarly journals Penetration Depth of Sodium Hypochlorite in Dentinal Tubules after Conventional Irrigation, Passive Ultrasonic Agitation and Nd:YAG Laser Activated Irrigation

2016 ◽  
Vol 7 (2) ◽  
pp. 105-111 ◽  
Author(s):  
Abdollah Ghorbanzadeh ◽  
Mohsen Aminsobhani ◽  
Khosro Sohrabi ◽  
Nasim Chiniforush ◽  
Sarvenaz Ghafari ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Sodium Hypochlorite ◽  
Nd:Yag Laser ◽  
Dentinal Tubules ◽  
Ultrasonic Agitation ◽  
Conventional Irrigation

RS 33 The smear layer and bacterial penetration of radicular dentinal tubules

1994 ◽  
Vol 20 (4) ◽  
pp. 196
Author(s):  
R.W. Hawkinson ◽  
C.T. Nii ◽  
E.L. Jacobsen ◽  
R.A. Murphy
Keyword(s):  
Smear Layer ◽  
Dentinal Tubules ◽  
Bacterial Penetration
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