scholarly journals Investigations on the Accumulation, Tissue Distribution and Toxicity of Silver Nanoparticles in Amammalian Model

2021 ◽  
Vol 4 (4) ◽  
pp. 34-52
Author(s):  
Sameh M.A. ◽  
Abdelmordy M.M. ◽  
Ahmed R.E. ◽  
Gamal M.M.
Keyword(s):  
Oxidative Stress ◽  
Current Work ◽  
Potential Toxicity ◽  
Total Proteins ◽  
Ag Nps ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Model Following ◽  
Target Organs ◽  
Main Target

The current work aimed to investigate the potential toxicity of Ag-NPs in male rabbits as a mammalian model, following acute and sub-acute dermal exposure terms. The rabbits were topically exposed to a single acute dose of 2.0% Ag-NPs and the assessments were conducted4 days latter, while a dose of 0.5% was administered every other day for the sub-acute exposure in two time periods (7 and 14 days). The accumulation and retention of Ag+ ions in the skin and their distribution in other vital tissues were measured. In addition, the concentrations of total proteins, lipidparameters and levels of different oxidative stress biomarkers in the skin were also evaluated. The results demonstrated the ability of Ag-NPs to penetrate the skin and accumulate rapidly in the dermal skin, then spread out to the blood and other vital organs. The spleen and liver seemed to be the main target organs. The high levels of Ag+ tissue accumulation affected the concentration of total proteins and acted as a stressor stimulus within the skin, thereby, initiating oxidative stress. The liberated ROS caused disruption of the activities of the antioxidant enzymes GST, GSH, GR, GPx, SOD and CAT as well as increased levels of H2O2 and MDA. The current work showed that the toxicity of Ag-NPs could be attributed to the release of Ag+ ions and the subsequent excessive generation of ROS.

scholarly journals Oxidative Stress Biomarkers in Urine of Metal Carpentry Workers Can Be Diagnostic for Occupational Exposure to Low Level of Welding Fumes from Associated Metals

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3167
Author(s):  
Flavia Buonaurio ◽  
Maria Luisa Astolfi ◽  
Daniela Pigini ◽  
Giovanna Tranfo ◽  
Silvia Canepari ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Occupational Exposure ◽  
Oxidation Products ◽  
Control Group ◽  
Welding Fumes ◽  
Oxidative Stress Biomarkers ◽  
Limit Values ◽  
Stress Biomarkers ◽  
Specificity And Sensitivity ◽  
The Impact

Urinary concentrations of 16 different exposure biomarkers to metals were determined at the beginning and at the end of a working shift on a group of workers in the metal carpentry industry. Five different oxidative stress biomarkers were also measured, such as the oxidation products of RNA and DNA metabolized and excreted in the urine. The results of workers exposed to metals were compared to those of a control group. The metal concentrations found in these workers were well below the occupational exposure limit values and exceeded the mean concentrations of the same metals in the urine of the control group by a factor of four at maximum. Barium (Ba), mercury (Hg), lead (Pb) and strontium (Sr) were correlated with the RNA oxidative stress biomarker, 8-oxo-7, 8-dihydroguanosine (8-oxoGuo), which was found able to discriminate exposed workers from controls with a high level of specificity and sensitivity. The power of this early diagnostic technique was assessed by means of the ROC curve. Ba, rubidium (Rb), Sr, tellurium (Te), and vanadium (V) were correlated with the level of the protein oxidation biomarker 3-Nitrotyrosine (3-NO2Tyr), and Ba, beryllium (Be), copper (Cu), and Rb with 5-methylcytidine (5-MeCyt), an epigenetic marker of RNA damage. These effect biomarkers can help in identifying those workers that can be defined as “occupationally exposed” even at low exposure levels, and they can provide information about the impact that such doses have on their health.

scholarly journals Antioxidants, lysosomes and elements status during the life cycle of sea trout Salmo trutta m. trutta L.

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Natalia Kurhaluk ◽  
Halyna Tkachenko
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Muscle Tissue ◽  
Salmo Trutta ◽  
Protein Carbonyl ◽  
Oxidative Modification ◽  
Oxidative Stress Biomarkers ◽  
Development Stages ◽  
Stress Biomarkers ◽  
Element Contents

AbstractThe aim of our study was to elucidate the effects of both development stages (parr, smolt, adult, spawner), and kelt as a survival form and sex (male, female) on the functional stability of the lysosomal complex, biomarkers of oxidative stress, and element contents in the muscle tissue of the sea trout (Salmo trutta m. trutta L.) sampled in the Pomerania region (northern Poland). We have evaluated the maximal activities of lysosomal enzymes (alanyl aminopeptidase, leucyl aminopeptidase, β-N-acetylglucosaminidase, and acid phosphatase), lipid peroxidation level, and protein carbonyl derivatives as indices of muscle tissue degradation. The relationship between lysosomal activity and oxidative stress biomarkers estimated by the lipid peroxidation level and protein carbonyl derivatives was also assessed, as well as the relationships between element levels and oxidative stress biomarkers. Trends of the main effects (i.e., the development stages and sex alone, the interaction of the sex and development stage simultaneously) on oxidative stress biomarkers, lysosomal functioning, and element contents in the muscle tissue were evaluated. The study has shown sex-related relationships between the pro- and antioxidant balance and the tissue type in the adult stage as well as modifications in the lysosomal functioning induced by long-term environmental stress associated with changing the habitats from freshwater to seawater and intense migrations. The highest level of toxic products generated in oxidative reactions and oxidative modification of proteins was noted in both the spawner stage and the kelt form. The holistic model of analysis of all parameters of antioxidant defense in all development stages and sex demonstrated the following dependencies for the level of lipid peroxidation, oxidative modification of proteins, lysosomal activities, and element contents: TBARS > OMP KD > OMP AD > TAC, AcP > NAG > LAP > AAP and Cu > Fe > Ca > Mn > Zn > Mg, respectively.

Could resistant starch supplementation improve inflammatory and oxidative stress biomarkers and uremic toxins levels in hemodialysis patients? A pilot randomized controlled trial

2018 ◽  
Vol 9 (12) ◽  
pp. 6508-6516 ◽  
Author(s):  
Marta Esgalhado ◽  
Julie A. Kemp ◽  
Renata Azevedo ◽  
Bruna R. Paiva ◽  
Milena B. Stockler-Pinto ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Resistant Starch ◽  
Controlled Trial ◽  
Hemodialysis Patients ◽  
Uremic Toxins ◽  
Oxidative Stress Biomarkers ◽  
Randomized Controlled ◽  
Stress Biomarkers ◽  
And Oxidative Stress ◽  
Pilot Randomized Controlled Trial

Prebiotic-resistant starch supplementation may be a good strategy to reduce inflammation, oxidative stress and uremic toxins in CKD patients.

Ameliorative Effects of Resveratrol on Oxidative Stress Biomarkers in Horses

2015 ◽  
Vol 35 (6) ◽  
pp. 518-523 ◽  
Author(s):  
Mary Ucheagha Ememe ◽  
Wayuta Philip Mshelia ◽  
Joseph Olusegun Ayo
Keyword(s):  
Oxidative Stress ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers

Caffeine impacts in the clam Ruditapes philippinarum: Alterations on energy reserves, metabolic activity and oxidative stress biomarkers

Chemosphere ◽  
2016 ◽  
Vol 160 ◽  
pp. 95-103 ◽  
Author(s):  
Diogo Cruz ◽  
Ângela Almeida ◽  
Vânia Calisto ◽  
Valdemar I. Esteves ◽  
Rudolf J. Schneider ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Metabolic Activity ◽  
Ruditapes Philippinarum ◽  
Energy Reserves ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
And Oxidative Stress
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