Isolation and Characterization of Flavonoids of the Ethanolic extracts of stems of Mimosa hamata (Willd.) by Chromatographic techniques

2021 ◽  
pp. 4602-4608
Author(s):  
N.I. Khan ◽  
B.C. Hatapakki ◽  
A.M. Tamboli
Keyword(s):  
Column Chromatography ◽  
Bioactive Compound ◽  
Solvent System ◽  
Chromatographic Techniques ◽  
Isolation And Characterization ◽  
Ethanolic Extracts ◽  
Chloroform Methanol ◽  
Identification And Characterization ◽  
Mass Spectroscopic Study

Since there are many bioactive compounds present in plant material containing various multi-component mixtures, their separation and determination is important to identify the active phytoconstituents responsible for pharmacological activity. Practically most of them have to be separated by column chromatographic techniques. The present study deals with the identification and characterization of bioactive principles from the stems of Mimosa hamata. The isolated fractions from the ethanolic extracts of stems of Mimosa hamata was carried out by column chromatography. For separation of a bioactive compound, the solvent system tried for column chromatography was Chloroform: Methanol in various ratios like 90:10, 80:20, 70:30, and 60:40 amongst these we could separate the first fraction at 80:20 and second fraction at 70:30. Two flavonoids compounds were isolated from the ethanolic extracts of stems of the medicinal plant Mimosa hamata (Willd). Based on chemical and spectral analyses their structures were elucidated as Quercetin and Cirsimaritin. From the above study it reveals the presence of flavonoids in the ethanolic extracts of stems of Mimosa hamata (Willd.) was isolated using column chromatography further subjected to characterization of isolated compounds including UV, IR, NMR and Mass spectroscopic study for elucidating the structure of the two separated compounds. The interpreted data concluded that, both isolated compounds are flavonoids i.e. Cirsimaritin and Quercetin. The pharmacological effect of Mimosa hamata stems may be due to the presence of its phytoconstituents flavonoids.

scholarly journals Isolation and Characterization of Lupeol from the Stem of Tapinanthus globiferus (A Rich.) and its Antimicrobial Assay

2020 ◽  
Vol 24 (6) ◽  
pp. 1015-1020
Author(s):  
V. Emaikwu ◽  
I.G. Ndukwe ◽  
R. Mohammed ◽  
O.R.A. Iyun ◽  
J.V. Anyam
Keyword(s):  
Spectral Analysis ◽  
Ethyl Acetate ◽  
Column Chromatography ◽  
Crude Extract ◽  
Solvent System ◽  
Pentacyclic Triterpenoid ◽  
Isolation And Characterization ◽  
Reported Data ◽  
Antimicrobial Assay

Lupeol, a pentacyclic triterpenoid, was isolated from hexane and ethyl acetate solvent system. In antiquity, the stem and leaf infusion of Tapinanthus globiferus has been used ethno-medicinally as a remedy for stomach ache, diarrhea, dysentery, and wounds. Lupeol isolation from this species was carried out by column chromatography after concentrating the crude extract using a rotary evaporator, and the structure was determined by analysis of the isolate by IR, 13CNMR, 1HNMR, HSQC, and HMBC spectral analysis as well as comparison with reported data. This is the first isolation of lupeol from the stem of this species. Keywords: Tapinanthus globiferus, Column chromatography, dysentery, Lupeol

Extraction, isolation and characterization of Bauhinia variegata flower

2020 ◽  
pp. 9-12
Author(s):  
Akanksha Gupta ◽  
Abhishek K Tripathi ◽  
Pushpraj S Gupta
Keyword(s):  
Biological Activities ◽  
Molecular Formula ◽  
Native Plant ◽  
Compound A ◽  
Active Constituents ◽  
Chromatographic Techniques ◽  
Isolation And Characterization ◽  
Soxhlet Apparatus ◽  
Bauhinia Variegata

Background: Bauhinia variegata Linn. is a native plant of Asia and China. B. variegata is found in tropical regions of the world. It belongs to family Leguminosae. It is used for diarrhea, hemorrhoids, constipation, piles, edema, leprosy, wounds, tumors, etc.  Objective: The objective of the present study was to perform extraction of B. variegata flower and isolation of active constituents from the extract. Materials and Methods: The ethanolic extraction of B. variegata flower was performed using the Soxhlet apparatus. The isolation of active constituents from the extract was performed using chromatographic techniques. In column chromatographic studies, n-hexane- [dichloromethane (DCM)] (2:8) was used as an eluting system and further purified through thin layer chromatography (TLC). Compound A and B were isolated through chromatographic techniques, then the molecular formula and characterization of these compounds were carried out with mass and infrared (IR) spectral analysis. Results and Discussion: The percentage yield of B. variegata ethanolic extract (BVE) was found to be 20.8% w/w. The different fractions were F1 having 12.5 grams with n-hexane, F2 (17.1 grams) with CH2Cl2, F3 (21.2 grams) with EtOAc, and F4 (13.4 grams) with EtOH. Compound A and B were isolated from the solvent fractions of n-hexane-DCM (2:8) and EtOAc-DCM (1:9), respectively. The compound A was characterized as 3-hydroxy-6-methoxy-2-phenyl-4H-chromen-4-one. The compound B was characterized as 3-hydroxy-6-methyl-2-phenyl-4H-chromen-4-one. Conclusion: Thus, B. variegata flowers possess active components that need to identify their biological activities.

scholarly journals Isolation and characterization of escherichia coli in ready-to-eat foods vended in Islamic University, Kushtia

1970 ◽  
Vol 18 ◽  
pp. 99-103 ◽  
Author(s):  
S Biswas ◽  
MAK Parvez ◽  
M Shafiquzzaman ◽  
S Nahar ◽  
MN Rahman
Keyword(s):  
Escherichia Coli ◽  
Pattern Analysis ◽  
University Campus ◽  
Rflp Pattern ◽  
E Coli ◽  
Fish Meat ◽  
Isolation And Characterization ◽  
Food Borne ◽  
Identification And Characterization

Context: Escherichia coli is shed in the feces of warm blooded animals and humans and thus potential for public health. Detection and characterization of E. coli in the ready-to-eat (RTE) foods concerns due to their presence indicates fecal contamination of the food.   Objective: To identify, characterize and RFLP pattern analysis of E. coli isolated from RTE foods vended in Islamic University campus, Kushtia.   Materials and Methods: Fifty samples from four types of consumed foods in six student halls of residence, some temporary restaurants of Islamic University, Kushtia were assessed for bacterial contamination by standard methods. Identification and characterization of E. coli isolates were performed using IMViC tests. Genomic DNA was used to perform RFLP pattern analysis.   Results: Thirty seven out of 50 (74%) examined samples of RTE foods had E. coli contamination. The highest number of E. coli was isolated from vegetable oriented RTE foods (90.90%) and fish, meat and cereals samples were also significantly E. coli positive. RFLP profiling of two E. coli isolates were observed.   Conclusion: The results of this study provide evidence that some RTE foods had unsatisfactory levels of contamination with E. coli. Thus street vended RTE food could be important potential vehicles for food-borne diseases. Molecular characterization may be exploited to identify food borne pathogen among different species.  Keywords: Ready-to-eat foods; Escherichia coli; RFLP pattern DOI: http://dx.doi.org/10.3329/jbs.v18i0.8783 JBS 2010; 18(0): 99-103

scholarly journals ANTI-BIOFILM POTENTIAL OF MENTHOL PURIFIED FROM MENTHA PIPERITA L. (MINT)

2020 ◽  
Vol 2020 (1) ◽  
Author(s):  
R Ejaz ◽  
S Malik ◽  
M Ahmad ◽  
H ALi ◽  
S Choudhry
Keyword(s):  
Column Chromatography ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Bioactive Compound ◽  
Solvent System ◽  
Mentha Piperita ◽  
Phytochemical Analysis ◽  
Antibacterial Drug ◽  
Biofilm Inhibition ◽  
Maximum Separation

Menthol, a bioactive compound of Mentha piperita (mint) with antibacterial properties was purified by column chromatography to determine its anti-biofilm potential. After phytochemical analysis, TLC was carried out using n-hexane: ethyl acetate: methanol: water (2:2:2:1) as the solvent system for ethanolic extract of mint. TLC achieved the maximum separation of mint constituents with Rf value of 0.68. A purified menthol fraction was obtained after silica gel column chromatography using four different eluting solvents. The menthol obtained was then used to perform biofilm inhibition assay to establish its antibacterial potential. Percentage inhibition was highest for bacillus subtilis (79.4%), as opposed to Pseudomonas aeruginosa (33.6%) and the combination of both bacteria (20%). ELISA reader was used to measure absorbance at 450-620nm and 630 nm. Using 450-620nm filter the values for percentage inhibition lies between 48.6-95% for standard and crude menthol samples. Similarly, at 630nm the values of inhibition lie between 23.4-70.6%. This anti-biofilm property of menthol can be utilized in antibacterial drug formulations.

scholarly journals Isolation and characterization of a γ-type phosphoinositide-specific phospholipase C (PLC-γ2)

1990 ◽  
Vol 269 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Y Homma ◽  
Y Emori ◽  
F Shibasaki ◽  
K Suzuki ◽  
T Takenawa
Keyword(s):  
Phospholipase C ◽  
Column Chromatography ◽  
Specific Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Deae Cellulose ◽  
Isolation And Characterization ◽  
Delta Type ◽  
Hydrolysis Of ◽  
Cellulose Column

A novel bovine spleen phosphoinositide-specific phospholipase C (PLC) has been identified with respect to immunoreactivity with four independent antibodies against each of the PLC isoenzymes, and purified to near homogeneity by sequential column chromatography. Spleen contains three of the isoenzymes: two different gamma-types [gamma 1 and gamma 2, originally named as PLC-gamma [Rhee, Suh, Ryu & Lee (1989) Science 244, 546-550] and PLC-IV [Emori, Homma, Sorimachi, Kawasaki, Nakanishi, Suzuki & Takenawa (1989) J. Biol. Chem. 264, 21885-21890] respectively] and delta-type of the enzyme, but PLC-gamma 1 is separated from the PLC-gamma 2 pool by the first DEAE-cellulose column chromatography. Subsequently, PLC-delta is dissociated on the third heparin-Sepharose column chromatography. The purified enzyme has a molecular mass of 145 kDa on SDS/polyacrylamide-gel electrophoresis and a specific activity of 12.8 mumol/min per mg with phosphatidylinositol 4,5-bisphosphate as substrate. This enzyme activity is dependent on Ca2+ for hydrolysis of all these phosphoinositides. None of the other phospholipids examined could be its substrate at any concentration of Ca2+. The optimal pH of the enzyme is slightly acidic (pH 5.0-6.5).

scholarly journals Isolation and characterization of piperine from the fruits of black pepper (Piper nigrum)

2014 ◽  
Vol 11 (1) ◽  
pp. 11-16 ◽  
Author(s):  
KC Saha ◽  
HP Seal ◽  
MA Noor
Keyword(s):  
1H Nmr ◽  
Column Chromatography ◽  
Mass Spectra ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Crystalline Compound ◽  
Food Preservative ◽  
Traditional Medicines ◽  
Isolation And Characterization

Black pepper (Piper nigrum) is a spice vine crop which is used as a food preservative and as an essential component in traditional medicines. The aim of this study was to extract, isolate and characterize the structure of piperine. The fruits of black pepper were extracted in ethanol and compounds present identified by TLC under iodine vapour. A compound (A2) having Rf value 0.54 was isolated by column chromatography which may be responsible for the pungency of black pepper. After recrystallization of the isolate with benzene and chloroform mixture (7:1) gave pale yellow crystals of m.p. 128~129oC (reported 130oC). The IR, 1H NMR and mass spectra of the pure crystalline compound (A2) were recorded. The mass spectra of the compound (A2) showed molecular ion peak M+· at 284.5 which was similar to that of piperine (mol. wt. 285). DOI: http://dx.doi.org/10.3329/jbau.v11i1.18197 J. Bangladesh Agril. Univ. 11(1): 11-16, 2013

scholarly journals Isolation and characterization of bioactive compound from

2021 ◽  
Vol 13 (1) ◽  
pp. 130-137
Author(s):  
H. J. Gunda ◽  
A.M. Yelwa ◽  
A.A. Shehu ◽  
M.M. Idris
Keyword(s):  
Bioactive Compound ◽  
Isolation And Characterization

No Abstract.

scholarly journals Molecular characterization of Nocardiopsis species from Didwana dry salt lake of Rajasthan, India

2021 ◽  
Vol 13 (1) ◽  
pp. 396-401
Author(s):  
Khushbu Parihar ◽  
Alkesh Tak ◽  
Praveen Gehlot ◽  
Rakesh Pathak ◽  
Sunil Kumar Singh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Bioactive Compounds ◽  
Salt Lake ◽  
Bioactive Compound ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Multiple Sequence ◽  
Isolation And Characterization

The genus Nocardiopsis is well known to produce secondary metabolites especially antibacterial bioactive compound. Isolation and characterization of bioactive compounds producing novel isolates from unusual habitats are crucial. The present study was aimed to explore Didwana dry salt lake of Rajasthan state in India for the isolation and characterization of actinomycetes. The isolated actinomycetes isolates were characterized based on culture characteristics, biochemical tests and 16S rRNA gene sequencing. The 16S rRNA gene sequence analysis revealed that all the five isolates inhabiting soil of the said dry salt lake of Didwana, Rajasthan belonged to four species of Nocardiopsis viz., N. synnemataformans, N. potens, N. prasina and N. dassonvillei subsp. albirubida. The molecular identification based on 16S rRNA gene sequences was found accurate and robust. The phylogram generated through multiple sequence alignment of all the test isolates of Nocardiopsis revealed that the isolates aroused from a single branch and validated monophyletic association. The present study is the first report of exploring Nocardiopsis isolates from the dry salt lake. These characterized Nocardiopsis isolates isolated from Didwana dry salt lake habitat are novel stains and can be of significance in the detection and utilization of novel bioactive compounds.

Synthesis of low-symmetry subphthalocyanines with diverse functionalization patterns

2009 ◽  
Vol 13 (02) ◽  
pp. 203-214 ◽  
Author(s):  
David González-Rodríguez ◽  
Christian G. Claessens ◽  
Tomás Torres
Keyword(s):  
Molecular Modeling ◽  
Nmr Spectra ◽  
Careful Analysis ◽  
Complete Separation ◽  
H Nmr ◽  
Chromatographic Techniques ◽  
Structural Assignment ◽  
Isolation And Characterization ◽  
Low Symmetry

We report here on the synthesis, isolation and characterization of unsymmetrically substituted subphthalocyanines obtained from the mixed condensation of 4,5-substituted phthalonitriles and/or 4-substituted phthalonitriles in the presence of BCl 3. Compared to phthalocyanines, it is clear that the cone-shaped structure of subphthalocyanines facilitates the isolation of the different regioisomers formed by regular laboratory chromatographic techniques. However, we demonstrate that some factors, such as the solubility of the regioisomers formed or the intermolecular interactions between side chains, can sometimes impede their complete separation. We also show that the structural assignment of each regioisomeric compound can be performed by careful analysis of its 1 H NMR spectra and, in some cases, with the help of NOE NMR experiments and molecular modeling.

scholarly journals Isolation and Characterization of Sclerienone C from Scleria Striatinux

2016 ◽  
Vol 11 (1) ◽  
pp. 1934578X1601100
Author(s):  
Kennedy D. Nyongbela ◽  
Felix L. Makolo ◽  
Thomas R. Hoye ◽  
Simon MN Efange
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Structure Elucidation ◽  
Spectroscopic Analysis ◽  
Methylene Chloride ◽  
Isolation And Purification ◽  
Isolation And Characterization ◽  
Combination Of Methods

Herein, we report the isolation and characterization of sclerienone C, a novel sesquiterpene isolated from the methylene chloride/methanol (1:1) extract of Scleria striatinux that we have deduced to have structure 1. This medicinal spice of Cameroon has been shown to display antimicrobial and antiplasmodial activities. The isolation and purification involved a combination of methods including silica gel column chromatography, Sephadex LH-20, and semi-prep HPLC separations. Structure elucidation was carried-out by means of spectroscopic analysis and comparison with previously isolated sesquiterpene derivatives from the plant.

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