scholarly journals In-Vitro Efficacy of Different Botanical Extract against Leaf Spot of Marry Gold caused by Alternaria alternat

2021 ◽  
Vol 1 (1) ◽  
pp. 05-08
Author(s):  
Keyword(s):  
Alternaria Alternata ◽  
Leaf Spot ◽  
Disease Incidence ◽  
Colony Growth ◽  
Garlic Extract ◽  
Ginger Extract ◽  
Neem Extract ◽  
Botanical Extracts ◽  
Botanical Extract

This study was carried out to study the in-vitroefficacy of different botanical extracts against leaf spot of marigold, caused by Alternaria alternata. The results of present study showed that the disease incidence from variouslocations of district HyderabadSindhwas observed Ghangra Mori35%, TandoHyder 30%, Rahooki25%, Moosa Khatyan20%, and Hosri15%.Upper limitdisease occurrencewas observedfrom Ghangra Mori and lower limit sickness was recordedfrom Hosri. The botanicalplantextract ‘Neem’ observed 48, 40and 34mmlinear colony growth of Alternaria alternataat 2%, 4% and 6% concentrations, respectively, Dhaturaextract observed 75.5, 63 and 57.5mmcolony growth of Alternaria alternataat 2%, 4% and 6% concentrations, respectively. Garlic extract observed 49%, 43% and 36% linear colony growth of Alternaria alternataat 2%, 4% and 6% concentrations, respectively. Ginger extract observed 50, 43.5and 36.5mmcolony growth of Alternaria alternataat 2%, 4% and 6% concentrations, respectively. Minimum linear colony growth of Alternaria alternatawas observed at 6% concentration for Neem extract followed by Garlic extract, Ginger extract and Datura extract, respectivelythe plant infusion, Neeminfusion show good effectoppositelinear colony growth of Alternaria alternataon6% solution

In-Vitro Efficacy of Different Fungicides Against Leaf Spot of Marigold Caused by Alternaria alternata

2021 ◽  
Vol 1 (1) ◽  
pp. 1-4
Author(s):  
Keyword(s):  
Lower Limit ◽  
Alternaria Alternata ◽  
Leaf Spot ◽  
Disease Incidence ◽  
Colony Growth ◽  
Good Effect ◽  
Upper Limit ◽  
Disease Occurrence ◽  
Chemical Fungicides

This study was carried out to study the in-vitro efficacy of different chemical fungicides leaf spot of marigold, caused by Alternaria alternata. The results of present study showed that the disease incidence from various locations of district Hyderabad Sindh was observed Ghangra Mori 35%, TandoHyder 30%, Rahooki 25%, Moosa Khatyan20%, and Hosri 15%. Upper limit disease occurrence was observed from Ghangra Mori and lower limit sickness was recorded from Hosri. The chemical fungicide Nativo observed 43%, 25.5% and 18.5% linear colony growth of Alternaria alternata at 100, 200 and 400 ppm, respectively. Aliete observed 52, 31.5 and 21 respectively. Carbandazm observed 52.5, 29.5 and 21mm respectively and Mancozeb observed 51, 28.5 and 20mm respectively. Lower limit growth of Alternaria alternata were judge in 400 ppm instead Nativo pursuited by Mancozeb, Carbandazm and Aliete, separately. In control there was 90mm was recorded. It was concluded that chemical fungicide ‘Nativo’ were full effective to stop the disperssion of Alternaria alternata in 400 ppm. Anytime the plant infusion, Neem infusion show good effect opposite linear colony growth of Alternaria alternata on 6% solution

scholarly journals Alternaria leaf spot of broccoli caused by Alternaria alternata in Bangladesh

2021 ◽  
Author(s):  
Sayma T. Nira ◽  
Md. Farhad Hossain ◽  
Nur Uddin Mahmud ◽  
Oliul Hassan ◽  
Md. Tofazzal Islam ◽  
...  
Keyword(s):  
Alternaria Alternata ◽  
Leaf Spot ◽  
Maximum Growth ◽  
Pathogenicity Test ◽  
Culture Studies ◽  
Alternaria Leaf Spot ◽  
Cultural Methods ◽  
Molecular Phylogenetic ◽  
Alternaria Sp

  This study aimed to isolate and characterise the pathogen associated with Alternaria leaf spot on broccoli and to evaluate the inhibitory effects of fungicides against it. We isolated and identified the fungal pathogen as Alternaria sp. using morphological and cultural methods. Based on the aligned sequences of the internal transcribed spacer (ITS) and molecular phylogenetic analysis by the neighbour-joining method, the isolates (Ab1 and Ab2) were confirmed as Alternaria alternata. The conidia of the isolates were dark brown, cylindrical, obclavate to muriform. The conidiophores were olivaceous brown, septate, and branched. The conidial morphology of the isolates ranged from 52.4–92.4 × 10–20 μm with 2–6 transverse and 0–3 longitudinal septa. Both isolates yielded positive results in the pathogenicity test on broccoli leaves by developing brown and circular spots with concentric rings on the leaves surrounded by yellow halos. The culture studies revealed that the maximum growth of the pathogen was obtained at 30 °C and pH 6.0. Tilt 250 WC showed the highest potential in suppressing the mycelial growth of the A. alternata in vitro at a concentration as low as 50 µg/mL. The results from this study contributed to the positive identification of the pathogen and characterised A. alternata as a destructive pathogen of broccoli which may be successfully controlled by the fungicide Tilt.  

scholarly journals First Report of Alternaria Leaf Spot of Almond Caused by Species in the Alternaria alternata Complex in California

Plant Disease ◽  
2001 ◽  
Vol 85 (5) ◽  
pp. 558-558 ◽  
Author(s):  
B. L. Teviotdale ◽  
M. Viveros ◽  
B. Pryor ◽  
J. E. Adaskaveg
Keyword(s):  
Alternaria Alternata ◽  
Leaf Spot ◽  
Disease Incidence ◽  
Prunus Dulcis ◽  
Leaf Spot Disease ◽  
Yield Losses ◽  
Mature Leaves ◽  
Branching Chains ◽  
Paper Towels ◽  
Industry Conference

A new leaf spot disease of almond (Prunus dulcis [Mill.] D. Webb) was observed in California in the late 1980s and was first associated with severe defoliation in the mid-1990s (1). Orchards in areas with frequent summer dews, high humidity, and little air movement sustained severe defoliation, resulting in yield losses often exceeding 50%. Symptoms occur only on leaf blades in late spring and summer. Lesions develop as small, circular, tan spots 1 to 3 mm in diameter that may enlarge to 5 to 20 mm in size. Semicircular lesions frequently develop along the leaf margins and tips. The centers of mature lesions become black with fungal sporulation. The fungi isolated from the margins of sporulating and non-sporulating lesions were identified as three species in the Alternaria alternata complex: A. alternata, A. arborescens, and A. tenuissima (2,3). Cultures grown in the dark on potato dextrose (PDA) or potato-carrot agar are grayish white to olivacious green in the former two species and dark gray and wooly in the latter species. On 5% PDA, cultures of all three species produced catenulate dictyospores that were granular to punctate (-verrucose), pale yellowish to brown or black, and had visible apical and basal pores. Conidial morphology depended on chain position; apical conidia ranged from ovoid to ellipsoid, whereas basal conidia were elliptical to obclavate. Average conidial dimensions of A. alternata and A. arborescens ranged from 20 to 28 × 8 to 10 μm. Conidia of A. alternata were produced in acropetal succession in branching chains on single, short suberect conidiophores. A. arborescens produced conidia similarly but mostly in dichotomously branching chains on short to long conidiophores. Average conidial dimensions of A. tenuissima ranged from 20 to 34 × 8 to 12 μm and they were produced in simple chains with one or two branches forming occasionally. In preliminary studies, the optimum temperature for mycelial growth on PDA for all three species ranged from 24 to 28°C. Fifty mature leaves on each of four 7- or 8-year-old almond cv. Butte trees were inoculated at 2- to 3-week intervals from mid-spring through summer in 1999 and 2000. Leaves were sprayed with aqueous suspensions containing 105 conidia per milliliter for one isolate each of A. alternata and A. arborescens and two isolates of A. tenuissima or with sterile distilled water. The shoots were covered for 72 h with plastic-lined brown paper bags containing wet paper towels. Leaves were examined for infection after 7 and 14 days. All isolates were pathogenic and produced non-sporulating lesions similar to those observed in natural infections. No symptoms were observed on noninoculated control plants. Disease incidence was low (<15%) until late June 1999 and July 2000. Inoculations in summer produced increasingly more infections, reaching incidences of 40 to 52% in September 1999 and 18 to 80% in August 2000. References: (1) J. E. Adaskaveg. 1994. Pages 5–7 in Proceedings of the 22nd Annual Almond Industry Conference. 1994. (2) J. Rotem. 1994. The genus Alternaria. Biology, Epidemiology, and Pathogenicity. APS Press, St. Paul, MN. (3) E. G. Simmons. Mycotaxon 70:325–369, 1999.

scholarly journals Screening of French Bean (Phaseolus vulgaris L.) Genotypes against Alternaria Leaf Spot Caused by (Alternaria alternata) under Dryland Conditions of Kashmir

2021 ◽  
pp. 25-30
Author(s):  
Sabiya Bashir ◽  
Mohammad Najeeb Mughal ◽  
Zahida Rashid ◽  
Shabeena Majid ◽  
Sabeena Naseer ◽  
...  
Keyword(s):  
Alternaria Alternata ◽  
Leaf Spot ◽  
Disease Incidence ◽  
French Bean ◽  
Phaseolus Vulgaris L ◽  
Dryland Agriculture ◽  
Disease Reaction ◽  
The Mean ◽  
Selection For ◽  
Resistant Genotypes

Sixty-three genotypes of  french bean was screened against leaf spot (Alternaria  alternata) in sick plots at Research Farm of Dryland Agriculture Research Srinagar, Rangreth during  Kharif  2018 and 2019. The highest mean disease incidence ranged from 0.00 to 85.00 per cent  with  the mean disease intensity ranged from 0.00 to 53.26 per cent .One genotype namely  ‘Local Pulwama’ was highly susceptible in their disease reaction. Among the screened germplasm, ‘Highly Resistant’ genotypes was SKU-R-601, SKUA-R-105, SKU-R-927, DARS-25, DARS-66, DARS-R-615,  while as ‘Susceptible’ genotypes was  DARS-8, DARS-12,  DARS-11, SKUAST-R-155, SKU-R-928, DARS-7, DARS-R-4, Bhaderwah (L),  Local  Kupwara black and Raj Jawala. Local Pulwama was found to be a highly susceptible (HS) genotype.  Twenty nine genotypes namely., DARS-16, DARS-9, DARS-54, DARS-39, VL-125, DARS-63, ENTO-504, SKUAST-204,SKU-R-925, DARS-60, DARS-109, DARS-43, DARS-44, SKU-R-23, DARS-4, DARS-74, SKU-R-105, DARS-40, DARS-23, DARS-18, SKU-R-71, WB-341, SKU-R-605, Uri local, Shopian (L), SKU-R-23, DARS-71, SSGB-729, DARS-R-19 showed resistant reaction to disease. The selection for resistance was based on the reaction of varieties on leaves.

scholarly journals Prevalence and Severity of Leaf Blight Disease of Chrysanthemum in Southern regions of Karnataka and in-vitro Fungicidal Effect on Alternaria alternata

2015 ◽  
Vol 9 (3) ◽  
pp. 38-45 ◽  
Author(s):  
G. Shamala ◽  
G. R. Janardhana
Keyword(s):  
Alternaria Alternata ◽  
Disease Incidence ◽  
Life Sciences ◽  
Leaf Blight ◽  
Cut Flowers ◽  
In Vitro Evaluation ◽  
Karnataka State ◽  
Commercially Important ◽  
Blight Disease

Chrysanthemum is one of the commercially important flower crop with great potential both as loose and cut flowers in Karnataka state. In the present study, prevalence and incidence of leaf blight disease was studied in six major growing districts of South Karnataka during 2012 - 2013. The pathogen associated with leaf blight disease was isolated and identified as Alternaria alternata. The study revealed that, the disease was distributed in Southern districts of Karnataka wherever Chrysanthemum is grown. The varieties Chandini and Karnel were more susceptible to blight showing highest disease incidence (100%) and severity (100%) recorded from Mandya and Mysore regions. The lowest disease incidence, severity and PDI of 18.40%, 38.68% and 24.89% were recorded on Rajawhite variety in Bangalore region. During 2013, the highest disease incidence, severity and PDI with 100%, 98.47%, and 45.71% were recorded in Mysore district on Chandini variety. In vitro evaluation of four fungicides on Alternaria alternata revealed that Carbendazim+Mancozeb was most effective with 95.65% inhibition followed by Carbendazim, Mancozeb and Metalaxyl+Mancozeb with 68.26%, 91.30% and 91.30% inhibition of the mycelium at 2.0% concentration.DOI: http://dx.doi.org/10.3126/ijls.v9i3.12465   International Journal of Life Sciences 9 (3): 2015; 38-45

scholarly journals Diagnosis And In Vitro Control Of Tomato Leaf Spot Caused By Alternaria alternata In Sulaimani Governorate

2018 ◽  
Vol 2ndInt.Conf.AGR (Special Issue) ◽  
pp. 637-650
Author(s):  
Jalal Hama salih Ismael Ismael ◽  
◽  
Shallaw Adulrahman Omer Omer ◽  
Keyword(s):  
Alternaria Alternata ◽  
Leaf Spot ◽  
Tomato Leaf

scholarly journals EVALUATION OF DIFFERENT ESSENTIAL OILS AND BIO CONTROL AGENTS AGAINST ALTERNARIA ALTERNATA THE CAUSAL AGENT OF FRUIT ROT OF JUJUBE

2020 ◽  
Vol 1 (1) ◽  
pp. 1-8
Author(s):  
Keyword(s):  
Growth Inhibition ◽  
Essential Oils ◽  
Alternaria Alternata ◽  
Colony Growth ◽  
Causal Agent ◽  
Biocontrol Agents ◽  
Fruit Rot ◽  
Pathogenicity Test ◽  
Neem Oil

This study was carried out on the evaluation of different essential oils and biocontrol agents against Alternaria alternata the causal agent of fruit rot. For the pathogenicity test against A. alternata was performed through cut and injection inoculation methods. The antifungal potential of different essential oils like turpentine, laung, neem and castus root was carried out at different doses i.e. 5, 10 and 15% by food poisoned method to find out the effective and suitable oil for the growth inhibition of A. alternata and evaluate the effect of some biocontrol agents on growth inhibition of A. alternata. The findings of this investigation stated that cut method of inoculation showed higher percentage (2.60) of rotting as compared to injection method of inoculation (2.35). Minimum colony growth of A. alternata (31.60, 21.25 and 15.16%) was examined under Laung oil at the dosage of 5, 10 and 15% followed by Neem oil (42.60, 31.60 and 21.30%), respectively. Maximum colony growth of A. alternata (62.71, 52.40 and 41.75%) was observed under Castus root oil at the dosage of 5, 10 and 15%. Zero growth of target pathogen was examined under Turpentine at 5, 10 and 15%. Under control the A. alternata showed (90 mm) colony growth. Minimum linear colony growth of A. alternata was observed for Hypoxylon Sp1 (50.31%), followed by Neurospora spp. (52.97%), Lasiodiplodia theobromae (54.7%), Chactomium subaffine (57.07%) and Fusarium sp. (65.4%). Maximum mycelial colony growth (90%) was recorded in control. Based on present investigation, Similarly, for controlling the linear colony growth of A. alternata under in vitro conditions Turpentine oil ranked 1st, Laung oil ranked 2nd, Neem oil ranked 3rd, Castus root oil ranked 4th.

scholarly journals Effect of Different Botanical Extracts and Organic Compound in The Management of Leaf Spot Disease of Strawberry (Fragaria ananassa Duch.) Caused by Pestalotia longisetula under Field Condition

2021 ◽  
Vol 9 (3) ◽  
pp. 193-202
Author(s):  
Beautina Karki ◽  
Hom Prasad Sitaula ◽  
Sandesh Bhandari ◽  
Pramod Gairhe ◽  
Hira Kaji Manandhar
Keyword(s):  
Disease Control ◽  
Leaf Spot ◽  
Disease Incidence ◽  
Leaf Spot Disease ◽  
Compost Tea ◽  
Treatment Control ◽  
Spot Disease ◽  
Cow Urine ◽  
Botanical Extracts ◽  
Stem Lesions

A field experiment was carried out using sweet sensation variety of strawberry to check the efficacy of different botanical extracts and organic compounds to manage leaf spot disease. The study was done using ten treatments viz; ginger (T1), turmeric (T2), garlic (T3), ginger + turmeric + garlic (T4), cow urine (T5), cow urine+ ginger + turmeric + garlic (T6), compost tea (T7), compost tea+ ginger+ turmeric+ garlic (T8), SAAF (mancozeb 63 % + carbendazim 12%) (T9) and control (T10), which was replicated thrice. The parameter observed during experiment were plant height, leaf number, disease leaves, stem lesions, yield, percent disease incidence, disease severity percent and percent disease control. At 75 days of transplantation, the highest plant height, leaf number (24.60) and yield (1391.67 gm/plot) were observed in treatment compost tea (18.14 cm), cow urine+ ginger+ turmeric+ garlic and garlic respectively and lowest in treatment control (16.19 cm, 23.27 & 566.67 gm/plot). The disease leaves and stem lesions were observed highest in treatment control (3.40&3.23) and lowest in treatments garlic (3.0), and cow urine+ ginger+ turmeric+ garlic (1.83) respectively at 75 days after transplantation. Moreover, the highest percent disease incidence and disease severity percent were observed highest in treatments control (28.94% & 84.81%) and percent disease control in treatment garlic (42.36%) at 75 days of transplantation. Therefore, it is suggested to use garlic extracts as a measure to control leaf spot disease of strawberry. Int. J. Appl. Sci. Biotechnol. Vol 9(3): 193-202.

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