scholarly journals Using of Microsatellites to Study the Genetic Polymorphisms of SRYm18 Region in Iraqi Sheep

2021 ◽  
Vol 13 (2) ◽  
pp. 32-37
Author(s):  
Hawraa Al-Mohamadawi ◽  
Asaad Y. Ayied
Keyword(s):  
Y Chromosome ◽  
Genetic Polymorphisms ◽  
Gene Segment ◽  
College Of Agriculture ◽  
Awassi Sheep ◽  
Expected Heterozygosity ◽  
The World ◽  
Rare Alleles ◽  
Equilibrium Test ◽  
Awassi Breed

The origin of animals is usually determined by their paternal genotypes of the genes on the Y chromosome. In addition to the genes and their polymorphisms in the genome of mitochondria that are inherited through dams. In view of the lack of studies focusing on the genes of the Y chromosome in the world and their absence in Iraq. The aim of the present study was to identify the multiple genetic polymorphisms of the SRYM18 gene in the Arabi and Awassi sheep raised in Iraq. The study was conducted in the Genetic Engineering Laboratories - College of Agriculture, University of Basra, as well as in the Basra Genome Laboratory. The amplification of the SRYM18 gene showed genetic polymorphisms and gave a gene segment of (103-880) bp. The number of alleles of the SRYm18 gene was 13 alleles in the Arabi and 16 alleles in the Awassi breeds. The equilibrium test showed that the two breeds were under equilibrium. The two breeds were identical with nine alleles, while the number of special alleles for the Arabi breed was two, while the Awassi breed was distinguished by five. The number of rare alleles reached 20, of which seven were of the Arabi breed, and 13 of them were of the Awassi breed. Mean expected heterozygosity was 0.6386 with nonsignificant Fis for Arabi breed (0.1541) but significant for Awassi breed (0.2213). Mean neutrality was close to lower bound (0.1721) and (0.1270) for Arabi and Awassi breeds respectively

scholarly journals Genetic Diversity of Salvia Species Assessed by ISSR and RAPD Markers

2016 ◽  
Vol 44 (2) ◽  
pp. 431-436 ◽  
Author(s):  
Masoumeh YOUSEFIAZARKHANIAN ◽  
Ali ASGHARI ◽  
Jafar AHMADI ◽  
Behvar ASGHARI ◽  
Ali Ashraf JAFARI
Keyword(s):  
Genetic Diversity ◽  
Genetic Polymorphisms ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
Genetic Variations ◽  
Molecular Techniques ◽  
Wild Populations ◽  
Similarity Coefficients ◽  
The World

The genus Salvia includes an enormous assemblage of nearly 1,000 species dispersed around the world. Due to possible threats to this genus, there is an immediate requirement to evaluate the diversity of its wild populations. ISSR and RAPD molecular techniques were used to evaluate the genetic relationships among twenty-one ecotypes of eight Salvia species. Amplification of genomic DNA using 23 primers (15 RAPD and eight ISSR) produced 280 bands, of which 91% were polymorphic. The results of marker parameters showed no clear difference between two marker systems. It was generally observed that both ISSR and RAPD markers had similar efficiency in detecting genetic polymorphisms with remarkable ability to differentiate the closely related ecotypes of Salvia. Nei’s similarity coefficients for these techniques ranged from 0.48 to 0.98. Based on the results of clustering, PCoA and AMOVA, the genetic diversity between and within species was confirmed. So, conservation and domestication of the genus Salvia must be due to levels of genetic variations.

scholarly journals Preface

2021 ◽  
Vol 904 (1) ◽  
pp. 011001
Keyword(s):  
Peer Review Process ◽  
Evaluation Process ◽  
College Of Agriculture ◽  
Double Blind ◽  
The World ◽  
Recent Developments ◽  
New Ideas ◽  
Research Findings ◽  
Combating Desertification ◽  
Desert Regions

The ICDS conference intends to bring together junior and senior, international and national researchers, scientists and scholars interesting in the field of desertification combat and water harvesting in desert regions. We think the conference will offer an excellent opportunity for networking with other members and exchange knowledge and explore the most recent developments in the desertification combat such as Water Resources Management in Desert Regions, Ecosystem and Biodiversity of Desert, Geology and Remote Sensing for Combating Desertification, Recent Agricultural Techniques Serving in Desert Soil Management, and Desert Regions Exploitation for Agricultural Production Regards. ICDS-2021 is a prestigious event organized by the Center of Desert Studies in cooperation with the College of Agriculture, University of Anbar, with a motivation to provide an excellent international platform for academicians, researchers, engineers, industrial participants and budding students around the world to share their research results with the global experts. We cordially invited participants from all over the world who wish to share their best practices and research findings in ICDS-2021 and enlighten their new ideas to make this event growing from strength to strength. Participants will be offered the opportunity to contribute to the conference in various roles: they can discuss keynotes, produce a poster presentation and submit full research or review articles. Articles submitted to the conference should report original high-quality and previously unpublished results. Submission of a manuscript implies that it is not under consideration for publication elsewhere. All submitted articles will be subjected to a double-blind peer-review process. Novelty, relevancy, language standards, references, and many other factors are considered for the evaluation process. The conference nominated Prof. Dr. Prof. Dr. Amir Ibrahim (College of Agriculture & Life Science Texas A&M University, U.S.), Prof. Dr. Medhat M. Elsahookie (College of Agriculture, University of Baghdad, Iraq), and Prof. Dr. Wadid Erian (Faculty of Agriculture, Cairo University, Egypt) as keynote speakers. ICDS-2021 will be held basically via physical attendance (In-person) for two days 17-18 November, 2021 in Ramadi, Anbar, Iraq. Each participant will be given approximately 10 minutes to view the most important findings of his/her research, and 5 minutes to discuss these results. Sincerely yours, Prof. Dr. Nihad Mohammed Aboud Director of the Center of Desert Studies/University of Anbar The Chairman of ICDS-2021 List of Committees (ICDS-2021) are available in this pdf.

scholarly journals Genetic analysis of silver-fir populations in the Beskids

2011 ◽  
Vol 72 (2) ◽  
pp. 115-119 ◽  
Author(s):  
Leon Mejnartowicz
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Analysis ◽  
Abies Alba ◽  
Genetic Distances ◽  
Silver Fir ◽  
Geographical Distance ◽  
Polymorphic Loci ◽  
Expected Heterozygosity ◽  
Rare Alleles

Twenty-eight isozymic loci were studied in the Beskid Mts., in four populations of common silver-fir (<em>Abies alba</em>): one in Beskid Makowski (BM) and three populations in Beskid Sądecki (BS). Their genetic variation and diversity were analyzed, and Nei's genetic distances between the populations were calculated. The results show that the geographical distance between the BM population and the three BS populations is reflected in genetic distances. The BM population is clearly distinct from the others. It has the lowest genetic diversity (<em>I</em> = <em>0.42</em>), percentage of polymorphic loci <em>(%PoL </em>= <em>64.29</em>) and number of rare alleles (<em>NoRa </em>= <em>5</em>). Besides, the BM population has the highest observed heterozygosity (<em>Ho </em>= <em>0.291</em>), which exceeds the expected heterozygosity (<em>He </em>= <em>0.254</em>), estimated on the basis of the Hardy-Weinberg Principle. On the contrary, BS populations are in the state of equilibrium, which is manifested, in similar values of <em>He </em>= <em>0.262 </em>and <em>Ho </em>= <em>0.264</em>.

scholarly journals Phylogenetic Analysis of the Darkfin Hind, Cephalopholis urodeta (Serranidae) Using Partial Mitochondrial CO1 Gene Sequences (Analisis Filogenetik Cephalopholis urodeta (Serranidae) Menggunakan Runutan Gen CO1 Mitokondria Parsial)

2015 ◽  
Vol 20 (1) ◽  
pp. 38 ◽  
Author(s):  
Yanti Ariyanti ◽  
Achmad Farajallah ◽  
Irma Shita Arlyza
Keyword(s):  
Pacific Ocean ◽  
Arabian Sea ◽  
North Pacific Ocean ◽  
Genetic Relationships ◽  
Gene Segment ◽  
Sampling Point ◽  
Tissue Samples ◽  
South Sulawesi ◽  
Separate Branch ◽  
The World

Cephalopholis merupakan salah satu genera terbesar dalam subfamili Epinephelinae yang memiliki banyak species. Secara fenotip, C. urodeta dewasa mirip dengan juvenil C. sonnerati karena memiliki ciri mencolok yaitu garis yang menyudut pada sirip ekor. Untuk memahami hubungan genetik pada spesies ikan ini, maka dilakukan analisis molekuler menggunakan ruas gen CO1. Sejumlah spesies ikan (famili Serranidae) dikumpulkan dari wilayah Sulawesi Selatan seperti Sinjai dan Kepulauan Selayar. Karakter fenotip diidentifikasi menggunakan buku katalog spesies kerapu dunia FAO, kemudian sampel yang diduga C. urodeta secara morfologi dipisahkan. Jaringan yang digunakan sebagai sumber DNA adalah jaringan otot bagian dorsal. Berdasarkan sebagian runutan gen CO1, diyakini bahwa sampel tersebut adalah C. urodeta. Runutan basa nukleotida dari sampel dibandingkan dengan 22 runutan basa nukleotida C. urodeta dari GenBank. Berdasarkan rekonstruksi pohon filogeni, C. urodeta dari Sinjai dan Kepulauan Selayar mengelompok dengan C. urodeta dari berbagai tempat seperti Polynesia, Mariana Utara, Filipina, pulau-pulau di sekitar Madagascar (Ouest, St. Gilles, Canyon, Cimetiere, Jaune) dan Adaman, sedangkan sampel dari Laut Arab di lepas pantai India berada pada cabang yang terpisah. Penelitian ini menyatakan bahwa C. urodeta yang melibatkan beberapa tempat dari berbagai perairan seperti Samudera Pasifik bagian Selatan (Polynesia), Samudera Pasifik bagian Utara (Northern Mariana), Laut China Selatan (Filipina), Teluk Bengal (Andaman), Laut Laccadive (reunion of Ouest, St. Gilles and Cimetiere), Laut Arab dan Indo Pasifik Barat (Indonesia) memiliki perbedaan jarak genetik yang kecil. Hal ini berimplikasi pada pemahaman pola migrasi spesies tersebut dan sebagai bahan pertimbangan pengambilan kebijakan konservasi. Kata kunci: Cephalopholis urodeta, CO1, filogenetik, Serranidae, Sulawesi Selatan Cephalopholis is one of the largest genera belonging to Subfamilly Epinephelinae, which has various species. Phenotypically, an adult C. urodeta similar to a juvenile of C. sonnerati, since both of them have a striking trait, two white oblique stripes or bands on the caudal fins. This work was conducted to investigate the genetic relationships of this species using CO1 gene segment. Fish were collected from several sampling point in South Sulawesi areas such as Sinjai and Selayar Island. The phenotypic characterizations were identified using the FAO species catalogue of groupers of the world, and the species that seemed to have C. urodeta morphology then separated. Tissue samples from dorsal muscle tissue were used as the source of DNA. Using part of the CO1 gene sequence, it can be confirmed that our samples are exactly C. urodeta species. The 22 C. urodeta sequences from GeneBank compared with our sequences. Interestingly, because based on the phylogenetic tree, our sequences clustered with the other C. urodeta sequences from several part of the world except the Arabian Sea off the coast of India, which is a separate branch. The present study reveals less genetic distance in C. urodeta than some other parts of the ocean as follows; South Pacific Ocean (Polynesia), North Pacific Ocean (Northern Mariana), South China Sea (Philippines), Andaman, west coast of Réunion Island, Arabian Sea and Indo West Pacific (Indonesia). This has implications for understanding the migration pattern of the species and may affect conservation policy decisions. Keywords: Cephalopholis urodeta, CO1, phylogenetics, Serranidae, South Sulawesi

scholarly journals Effect of IGF-1 and GH Genes Polymorphism on Weights and Body Measurements of Awassi Lambs in Different Ages

2019 ◽  
Vol 32 (1) ◽  
pp. 39-46
Author(s):  
Rahman H. Al Qasimi ◽  
Amad F. Hassan ◽  
Bassam Y. Khudair
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Genetic Analysis ◽  
Body Measurements ◽  
College Of Agriculture ◽  
Awassi Sheep ◽  
Superior Genotype ◽  
Weaning Age ◽  
Productive Traits ◽  
Awassi Lambs

The study was carried on 68 ewes of local Awassi sheep in the Al-Kafeel sheep station Karbala governorate, Iraq for the period from 1/10/2017 to 1/8/2018. Genetic analysis was carried out in the molecular genetics laboratory at the College of Agriculture / Basrah University in order to extract (DNA) and determine the genotype  of the IGF-1(Insulin-like growth factor 1) and GH (Growth Hormone) genes. This study aimed to know the association between the genotype of the ewes of IGF-1 and GH  genes and their relationship to the productive traits of the lambs, which included the weights of lambs from birth to weaning and body measurements, Three of the  genotypes for IGF-1 were revealed AG, GC, CC and two for GH CT, TC . The effect of IGF-1 genotypes was significant (P<0.05) on weights of lambs at weaning, age  6 months and some body measurements with superior genotype GC. The genotypes of  GH had a significant effect on the weight of the lambs at birth by CT superiority. While the weights of weaning, six months and body measurements were not significantly affected by different genotypes.

scholarly journals Assessment of genetic diversity in tomato landraces using ISSR markers

Genetika ◽  
2016 ◽  
Vol 48 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Mashhid Henareh ◽  
Atilla Dursun ◽  
Babak Abdollahi-Mandoulakani ◽  
Kamil Haliloğlu
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Issr Markers ◽  
Vegetable Crops ◽  
North West ◽  
Expected Heterozygosity ◽  
The World ◽  
Commercial Cultivars ◽  
The Mean ◽  
Issr Primers

Tomato is one of the most economically important vegetable crops in many parts of the world. Turkey and Iran are the main producers of tomatoes in the world. The objective of this study was to assess the genetic variation of 93 tomato landraces from East Anatolian region of Turkey and North-West of Iran, along with three commercial cultivars using 14 ISSR primers. The percentage of polymorphic loci (PPL) for all primers was 100%. The mean of expected heterozygosity (He) for the primers varied from 0.153 (UBC808) to 0.30 (UBC848). The dendrogram placed the landraces and commercial cultivars into nine groups. The genotypes originating from the same region, often located in the same group or two adjacent groups. The highest likelihood of the data was obtained when population were located into 2 sub-populations (K = 2). These sub-populations had Fst value of 0.16 and 0.21.

scholarly journals Effect of fat tail docking on meat quality of Awassi sheep in comparison with Lacaune sheep

2019 ◽  
pp. 6793-6804
Keyword(s):  
Meat Quality ◽  
Gluteus Medius ◽  
Biceps Femoris ◽  
Drip Loss ◽  
Fat Tail ◽  
Awassi Sheep ◽  
Tail Docking ◽  
Three Samples ◽  
One Year ◽  
Awassi Breed

1 ABSTRACT This experiment was conducted to study the effect of fat tail docking on Awassi lamb sheep’s meat in comparison with thin-tail Lacaune sheep’s meat (imported breed in Lebanon). Twenty seven ram lambs were divided into three groups, intact fat-tail Awassi (IA), docked fat-tail Awassi (DA), and imported thin-tail Lacaune (IL). Docking was performed at one week of age, by applying tight rubber rings on the tail. Animals having the same body weight were slaughtered at one year of age. Three samples of muscles were excised from each carcass; Biceps Femoris (Bf), Longissimus Dorsii (Ld) and Gluteus Medius (Gm). Samples were wrapped in an oxygen permeable film and stored at 4°C and - 30°C for the assessment of pH, and meat quality traits. The ultimate pH was significantly higher (P<0.01) in Lacaune breed as compared to both docked and intact Awassi breed. DA animals had significantly higher (P<0.01) fat content in Ld muscle than IA and IL lambs. Both Awassi groups presented higher L* values than Lacaune animals (P< 0.05). In addition for Awassi breed, L* values of Bf and Gm muscles were significantly greater (P< 0.05) than that of Ld muscle. b* values were significantly greater (P< 0.05) in Gm of all animals under study. Lacaune group had lower drip loss values than Awassi groups (P< 0.05 in Bf and Gm muscles and P< 0.01 in Ld muscle). However for Awassi breed, the drip loss in Ld muscle of DA animals was significantly lower (P<0.01) than IA ones. Furthermore, thawing and cooking losses were neither affected by breed nor by docking. Regarding the PND values, the Ld muscle of DA group presented higher values (P< 0.05) than Bf and Gm muscles of both intact groups. In addition, cooked meat PND values were significantly higher (P< 0.05) in Ld muscle of DA when compared to intact groups. Due to the scarcity of information in this area of research, further investigations are needed.

scholarly journals Genetic Polymorphisms and Forensic Efficiencies of a Set of Novel Autosomal InDel Markers in a Chinese Mongolian Group

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Wenqing Zhang ◽  
Xiaoye Jin ◽  
Yijie Wang ◽  
Tingting Kong ◽  
Wei Cui ◽  
...  
Keyword(s):  
Genetic Polymorphisms ◽  
Genetic Relationships ◽  
Mean Values ◽  
The Past ◽  
Asian Populations ◽  
Expected Heterozygosity ◽  
Indel Markers ◽  
Hardy Weinberg Equilibrium ◽  
The Mean ◽  
Genetic Structures

Insertion/deletion (InDel) markers have been treated as a prospective and helpful aid in the fields of forensic human identifications and biogeography origin researches for the past few years. In this study, we analyzed genetic polymorphisms and forensic efficiencies of 35 InDels in a novel multiplex PCR-InDel panel in a Chinese Mongolian group. All these 35 InDel loci were observed to conform to Hardy–Weinberg equilibrium and linkage equilibrium. The mean values of expected heterozygosity and observed heterozygosity were 0.4788 and 0.4852, respectively. Besides, the interpopulation differentiations and genetic distributions based on 35 InDels found that the Chinese Mongolian group might have closer genetic relationships and similar population genetic structures with East Asian populations.

Estimates of gene flow from rare alleles in natural populations of medfly Ceratitis capitata (Diptera: Tephritidae)

2004 ◽  
Vol 94 (5) ◽  
pp. 449-456 ◽  
Author(s):  
A. Kourti
Keyword(s):  
Gene Flow ◽  
Ceratitis Capitata ◽  
Natural Populations ◽  
Clinal Variation ◽  
African Populations ◽  
The World ◽  
Rare Alleles ◽  
Geographical Populations ◽  
Latitudinal Clines ◽  
North And South

AbstractGene flow based on the spatial distribution of rare alleles at 25 gene loci was estimated in 15 populations of Ceratitis capitata (Wiedemann) from different parts of the world. Estimates of Nm, the number of migrants exchanged per generation among populations in different regions of the world, appeared to be quite similar, ranging from 3.36 in tropical Africa to 2.94 in the New World and 2.72 in Mediterranean basin populations. This suggests that gene flow among neighbouring populations of medfly is quite extensive. The genetic differentiation in American, Mediterranean and African populations was related to major climatic differences between North and South. These differences arise mainly from five loci that showed gene frequency patterns suggestive of latitudinal clines in allele frequencies. The clinal variation was such that tropical-subtropical populations were more heterozygous than temperate populations. It was concluded that gene flow, counteracting the forces of natural selection and genetic drift, determines the extent to which geographical populations of C. capitata are differentiated.

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