scholarly journals Active sites of peptide from Arg-Ser-Ser protect against oxidative stress in HepG2 cells

eFood ◽  
2021 ◽  
Author(s):  
Jiaxi Liang ◽  
Ruier Zhang ◽  
Jiaxin Zhao ◽  
Huifan Liu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Active Site ◽  
Hepg2 Cells ◽  
Active Sites ◽  
Orbital Energy ◽  
Enzymatic System ◽  
Quantum Chemistry Calculations ◽  
Antioxidative Effects ◽  
Pre Treatment

Peptide Arg-Ser-Ser (RSS) was derived from Lactobacillus amylolyticus co-incubated with edible <i>Dendrobium aphyllum</i>. Here, we further examined the antioxidative effects of RSS in HepG2 cells subjected to 2,2-azobis(2-methylpropanimidamidine) dihydrochloride-induced oxidative stress. RSS protected cells by eliminating the level of reactive oxygen species (ROS). The protein expression of antioxidant enzymes, Nrf2 and Keap1 determined by western blot, indicated that RSS might maintain cellular homeostasis by directly scavenging free radicals instead of by enzymatic system. Furthermore, quantum chemistry calculations and a characterization of electronic-related properties showed that the highest occupied molecular orbital energy distribution was on arginine residue. Pre-treatment with RSS with the active site methylated resulted in increased ROS levels, thereby verifying that N<sub>2</sub>-H<sub>3</sub> is the active site for antioxidant activity. Our findings provide valuable insights into the antioxidant activity of RSS and a basis for developing antioxidant functional foods.

scholarly journals ANTIOXIDANT ACTIVITY AND ANTIPROLIFERATIVE ACTION OF METHANOLIC EXTRACT OF LIQUORICE (GLYCYRRHIZA GLABRA) IN HEPG2 CELL LINE

2016 ◽  
Vol 8 (9) ◽  
pp. 293 ◽  
Author(s):  
Dasharath B. Shinde ◽  
Santosh S. Koratkar ◽  
Neeti Sharma ◽  
Ajinkya A. Shitole
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Hepg2 Cells ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Glycyrrhiza Glabra ◽  
Total Phenolic ◽  
Hepg2 Cell Line

<p><strong>Objective: </strong>To evaluate the <em>in vitro </em>antioxidant activity of liquorice (<em>Glycyrrhiza glabra) </em>against H<sub>2</sub>O<sub>2</sub> induced oxidative stress in HepG2 cell line.</p><p><strong>Methods: </strong>Antioxidant activity of methanolic extracts of <em>Glycyrrhiza glabra</em> was investigated by measuring total phenolic content using folin-ciocalteu reagent (FCR), free radical scavenging activity by DPPH and ferric reducing antioxidant power (FRAP). The presence of phenolic compounds and flavonoids in the extract was confirmed by Liquid Chromatography-Mass Spectrometry (LC-MS) analysis. Furthermore, the protective effect of methanolic extract of <em>Glycyrrhiza glabra</em> against oxidative stress induced by H<sub>2</sub>O<sub>2 </sub>in HepG2 cells was investigated by MTT assay. HepG2 cells were exposed with five different treatments viz. liquorice, H<sub>2</sub>O<sub>2</sub>, ascorbic acid, H<sub>2</sub>O<sub>2</sub>+liquorice and H<sub>2</sub>O<sub>2</sub>+ascorbic acid, to explore the effect of the extract on malondialdehyde (MDA) production, catalase activity, and glutathione reductase levels.<strong></strong></p><p><strong>Results: </strong>The total phenolic content estimated in <em>Glycyrrhiza glabra </em>extract was found to be 241.47 µg per 1000 µg/ml of methanolic extract. It was found that as the concentration of the extract was increased both the free radical scavenging activity and ferric ion reducing power was also found to increase. LC-MS analysis confirmed the presence of eight different phenolic compounds in the methanolic extract which are possibly contributing to the antioxidant activity exhibited by the extract. It was also observed that liquorice treated HepG2 cells showed lower MDA and higher glutathione and catalase levels as compared to only H<sub>2</sub>O<sub>2 </sub>treated HepG2 cells where increased MDA production, decreased glutathione reductase and catalase production was observed.</p><p><strong>Conclusion: </strong>Our results thus conclude that, the methanolic extract of <em>Glycyrrhiza glabra </em>can be used as natural supplements in various disease conditions where oxidative stress has been reported. <strong></strong></p><p> </p>

Effect of soybean peptides against hydrogen peroxide induced oxidative stress in HepG2 cells via Nrf2 signaling

2020 ◽  
Vol 11 (3) ◽  
pp. 2725-2737 ◽  
Author(s):  
Guofu Yi ◽  
Jalal ud Din ◽  
Fen Zhao ◽  
Xinqi Liu
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Hepg2 Cells ◽  
Soybean Protein ◽  
Protein Hydrolysates

The aim of this study was to determine the effects of soybean protein hydrolysates against intracellular antioxidant activity.

scholarly journals Antioxidant activity of soybean peptides on human hepatic HepG2 cells

2019 ◽  
Vol 7 ◽  
Author(s):  
Lammi Carmen ◽  
Carlotta Bollati ◽  
Anna Arnoldi
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Hepg2 Cells ◽  
Functional Foods ◽  
Bioactive Peptides ◽  
Oxygen Species ◽  
Cellular Lipid ◽  
Dpph Radical ◽  
Significant Augmentation ◽  
Pre Treatment

Soybean is an interesting source of bioactive peptides useful for the development of functional foods and nutraceuticals. In this study, the antioxidant activity of peptic (P) and tryptic (T) soybean hydrolysates was characterized. Results suggest that both hydrolysates are able to scavenge DPPH radical. Moreover, after induction of oxidative stress by using H2O2, either Soybean P or T pre-treatment reduces the ROS, lipid peroxidation, and NO levels in human hepatic HepG2 cells. HepG2 cells, exposed to H2O2 alone, produce a significant augmentation of intracellular reactive oxygen species (ROS) levels by 29.5%, with the consequence of an augmentation of cellular lipid peroxidation levels up to 112.4±0.5%. The pre-treatment with soybean hydrolysates restores the basal level of ROS and induces a reduction of cellular lipid peroxidation. The antioxidant ability of Soybean P and T are also confirmed by their ability to reduce the H2O2-induced NO levels in HepG2 cells.

scholarly journals Oxidative and Antioxidative Effects of Desflurane and Sevoflurane on Rat Tissue in Vivo

2011 ◽  
Vol 62 (2) ◽  
pp. 113-119 ◽  
Author(s):  
Hülya Türkan ◽  
Ahmet Aydin ◽  
Ahmet Sayal ◽  
Ayşe Eken ◽  
Cemal Akay ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Critical Illness ◽  
Surgical Trauma ◽  
Anaesthetic Gases ◽  
Antioxidative Effects ◽  
Rat Tissue ◽  
Cu And Zn

Oxidative and Antioxidative Effects of Desflurane and Sevoflurane on Rat Tissuein VivoGeneral anaesthetics are often used in patients who are under oxidative stress due to a critical illness or surgical trauma. Some anaesthetics may worsen oxidative stress and some may act as antioxidants. The aim of this study was to evaluate liver, brain, kidney, and lung tissue oxidative stress in rats exposed to desflurane and sevoflurane and in unexposed rats. The animals were divided in three groups: control (received only air); sevoflurane (8 %), and desflurane (4 %). After four hours of exposure, we evaluated the levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), Cu, and Zn. Exposure to either of the anaesthetics significantly increased lung MDA levels compared to control (Mann-Whitney U test; P<0.05), probably because it is the tissue directly exposed to anaesthetic gases. Oxidative stress and antioxidant activity in other tissues varied between the desflurane and sevoflurane groups. Our results suggest that anaesthesiologist should not only be aware of the oxidative or antioxidative potential of anaesthetics they use, but should also base their choices on organs which are the most affected by their oxidative action.

scholarly journals Curcumin Nanoparticles Protect against Isoproterenol Induced Myocardial Infarction by Alleviating Myocardial Tissue Oxidative Stress, Electrocardiogram, and Biological Changes

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2802 ◽  
Author(s):  
Paul-Mihai Boarescu ◽  
Ioana Boarescu ◽  
Ioana Corina Bocșan ◽  
Raluca Maria Pop ◽  
Dan Gheban ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Myocardial Infarction ◽  
Curcuma Longa ◽  
Myocardial Damage ◽  
Qrs Complex ◽  
Curcumin Nanoparticles ◽  
Myocardial Oxidative Stress ◽  
Antioxidative Effects ◽  
Pre Treatment ◽  
Higher Doses

Curcumin from Curcuma longa is a nutraceutical compound reported to possess strong antioxidant activity that makes it a candidate for use in counteracting oxidative stress-induced damage. The effect of pre-treatment with curcumin nanoparticles (nC) compared to conventional curcumin (Cs) on blood pressure, electrocardiogram, and biological changes on isoproterenol (ISO)-induced myocardial infarction (MI) in rats had been investigated. The Cs doses of 150 and 200 mg/kg bw and all nC doses (100, 150 and 200 mg/kg bw) significantly reduced heart rate before ISO administration and prevented QRS complex enlargement after MI induction (p < 0.026). All doses of Cs and nC prevented prolongation of the QT and QT corrected (QTc) intervals, with better results for higher doses (p < 0.048). The nC solution had more significant results than Cs in all metabolic parameters assessed (lactate dehydrogenase, glycaemia, aspartate transaminase, and alanine transaminase, p < 0.009). nC was more efficient than Cs in limiting myocardial oxidative stress and enhancing antioxidative capacity (p < 0.004). Compared to Cs, nC better prevented myocardial damage extension, reduced interstitial oedema, and inflammation. Curcumin nanoparticles as compared to conventional curcumin exert better antioxidative effects. Moreover, nC better prevent cardiomyocytes damage, and electrocardiogram alterations, in the case of ISO-induced MI in rats.

scholarly journals Discovering active sites in peptide Ala–Val–Thr–Phe that counter 2,2-azobis(2-methylpropanimidamidine)dihydrochloride-induced oxidative stress in HepG2 cells

RSC Advances ◽  
2020 ◽  
Vol 10 (41) ◽  
pp. 24444-24453
Author(s):  
Jiaxi Liang ◽  
Qin Wang ◽  
Jianliang Liu ◽  
Guozhong Huang ◽  
Churong Liang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepg2 Cells ◽  
Active Sites ◽  
Potential Mechanism ◽  
Dendrobium Aphyllum

The potential mechanism underlying the cytoprotective effects of the Ala–Val–Thr–Phe (AVTF) peptide derived from edible Dendrobium aphyllum was co-incubated with Lactobacillus amylolyticus.

Antioxidant activity of papaya seed extracts against H2O2 induced oxidative stress in HepG2 cells

LWT ◽  
2016 ◽  
Vol 66 ◽  
pp. 293-297 ◽  
Author(s):  
Swetha Salla ◽  
Rajitha Sunkara ◽  
Simon Ogutu ◽  
Lloyd T. Walker ◽  
Martha Verghese
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Hepg2 Cells ◽  
Papaya Seed ◽  
Seed Extracts

scholarly journals Antioxidant activity of SSeCAHK in HepG2 cells: a selenopeptide identified from selenium-enriched soybean protein hydrolysates

RSC Advances ◽  
2021 ◽  
Vol 11 (54) ◽  
pp. 33872-33882
Author(s):  
Jian Zhang ◽  
Qiyue Zhang ◽  
He Li ◽  
Xinwei Chen ◽  
Wanlu Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Hepg2 Cells ◽  
Soybean Protein ◽  
Protein Hydrolysates ◽  
Natural Food ◽  
Protective Effects

Se-containing antioxidative soybean peptides were isolated and identified as SSeCAHK. The SSeCAHK had protective effects against H2O2-induced oxidative stress in HepG2 cells and could be used as a natural food-born antioxidant.

Picking pockets to fuel antimicrobial drug discovery

2007 ◽  
Vol 35 (5) ◽  
pp. 980-984 ◽  
Author(s):  
W.N. Hunter
Keyword(s):  
Oxidative Stress ◽  
Enzyme Activity ◽  
Drug Discovery ◽  
Active Site ◽  
Drug Target ◽  
Active Sites ◽  
Microbial Pathogens ◽  
Apicomplexan Parasites ◽  
Trypanosomatid Parasites ◽  
The Right

The inhibition of essential enzymes in microbial pathogens offers a route to treatment of infectious diseases. However, although the biology of the organism dictates a need for a particular enzyme activity, this does not necessarily mean that the enzyme is a good drug target. The chemistry of the active site (size, shape and properties) determines the likelihood of finding a molecule with the right properties to influence drug discovery. Discriminating between good and less-good targets is important. Studies on enzymes involved in the regulation of oxidative stress and pterin/folate metabolism of trypanosomatid parasites and isoprenoid precursor biosynthesis in bacteria and apicomplexan parasites illustrates a range of active sites representing those that are challenging with respect to the discovery of potent inhibitors, to others that provide more promising opportunities in drug discovery.

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