DENTAL GERM FORMATION AS INFLUENCED BY 5-FLUOROURACIL (5-FU) IN NEWBORN RATS

Foi estudada a influência da droga antimitótica 5-fluoruracil (5-FU), administrada a ratas prenhes sobre a formação do germe dental de ratos recém nascidos. Foram utilizadas 125 fêmeas com 90-100 dias de idade e 15 machos adultos (Rattus rattus norvaegicus, albinus, Wistar). A droga foi injetada por via intraperitoneal a ratas grávidas, em doses únicas, aos 9º, 10º, 11º , 12º ou 13 º dias de gestação. Os animais foram divididos em quatro subgrupos de acordo com a dose administrada: 10, 20, 30 e 40 mg/kg. Os resultados mostraram que nessas doses, o 5-FU não teve efeito tóxico nas ratas prenhes, alem de perda de peso com doses mais elevadas (30-40 mg/kg), enquanto causou anomalias dentais nos embriões, bem como malformações externas. A proporção de tais anomalias foi, alem de dose dependente, influenciada pelo dia de gestação no qual antimitótico foi administrado às mães: foi observada maior sensibilidade ao 9º dia e menor ao 10º dia. Doses mais elevadas (30-40 mg/kg) causaram freqüentemente morte do embrião e reabsorção fetal. Agenesia do germe dentário foi freqüentemente observada. Abstract It was studied the influence of the antimitotic drug 5 - fluorouracil (5-FU), administered to pregnant rats, on dental germ formation of newborn rats. A total of 125 female aged 90-100 days and 15 male adult rats (Rattus rattus norvaegicus, albinus, Wistar) were used. The drug was injected intraperitoneally in single doses to females at the 9th, 10th, 11th, 12th and 13th days of pregnancy. The animals were divided in four groups according to the doses administered: 10, 20, 30 and 40 mg/kg. Results show that the drug induced no toxicity to mothers, other than loss of body weight with higher doses (30-40 mg/kg), but caused in the fetuses dental germ anomalies and body external malformations. The proportion of such anomalies were, besides dose dependent, influenced by the day of pregnancy when the drug was administered to the mothers: there was a greater sensitivity at the 9th day and a lesser one at the 10th day. Higher doses (30-40 mg/kg) caused frequently embryonic death or fetal uterine reabsorption. Agenesia of the dental germ was frequently found.

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Pharmacological characterization of a novel oestrogen antagonist, ZK 119010, in rats and mice

Journal of Endocrinology ◽

10.1677/joe.0.1300409 ◽

1991 ◽

Vol 130 (3) ◽

pp. 409-414 ◽

Cited By ~ 26

Author(s):

Y. Nishino ◽

M. R. Schneider ◽

H. Michna ◽

E. von Angerer

Keyword(s):

Dependent Manner ◽

Adult Rats ◽

Pharmacological Characterization ◽

Once Daily ◽

Uterine Growth ◽

Steroidal Compound ◽

Rats And Mice ◽

Dose Dependent ◽

Higher Doses

ABSTRACT The oestrogenic and antioestrogenic effects of a new non-steroidal compound ZK 119010(2-(4-hydroxyphenyl)-3-methyl-1-[6-(1-pyrrolidinyl)-hexyl]-indol-5-ol) were evaluated and compared with those of tamoxifen and ICI 164384. In immature mice, ZK 119010 administered once daily for 3 days (s.c.) inhibited the uterotrophic and vaginotrophic effect of oestradiol in a dose-dependent manner and was distinctly more potent than tamoxifen or ICI 164384 in exerting antioestrogenic effects. When antioestrogens in combination with oestradiol were administered once daily for 5 days to ovariectomized adult rats, ZK 119010 at lower doses (≤ 1 mg/kg) was slightly less effective than tamoxifen in inhibiting the uterotrophic effect of oestradiol. At the higher doses, however, ZK 119010 was strongly antioestrogenic, and ICI 164384 was less effective than ZK 119010 or tamoxifen. ZK 119010 at 10 mg/kg, like ICI 164384 at 30 mg/kg, caused an almost complete inhibition of the oestradiol-induced uterine growth in rats. The antioestrogenic effect of tamoxifen in rats was also limited by its inherent oestrogenic property. The oestrogenic activity of ZK 119010 was much below that of tamoxifen, whereas ICI 164384 did not show oestrogenicity. The present results indicate that ZK 119010 is a novel type of non-steroidal antioestrogen which has only a marginal oestrogenic effect in rats and mice. Such an antioestrogen may be useful for the treatment of oestrogen-sensitive diseases in man. Journal of Endocrinology (1991) 130, 409–414

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Ontogeny of thyroid peroxidase activity in perinatal rats

Acta Endocrinologica ◽

10.1530/acta.0.1010397 ◽

1982 ◽

Vol 101 (3) ◽

pp. 397-402 ◽

Cited By ~ 1

Author(s):

Y. Fukiishi ◽

T. Harauchi ◽

T. Yoshizaki ◽

Y. Hasegawa ◽

Y. Eguchi

Keyword(s):

Peroxidase Activity ◽

Thyroid Peroxidase ◽

Newborn Rats ◽

Adult Rats ◽

Pregnant Rats ◽

Maternal Treatment ◽

Adult Females ◽

Cell Hypertrophy ◽

Foetal Life ◽

Preferential Synthesis

Abstract. The ontogeny of thyroid peroxidase (TPO) activity was investigated in rat foetuses and neonates. From day 19 to 22 of gestation in intact pregnant rats, the TPO activity in their foetuses increased with foetal age. Following maternal treatment with propylthiouracil (PTU), the TPO activity markedly increased in foetuses on and after day 20 of gestation. The TPO activity in encephalectomized foetuses increased as markedly as that in intact littermates, whereas that in hypophysectomized littermates failed to increase. Newborn rats nursed by mothers treated with PTU had a TPO activity similar to that in controls of untreated mothers. There was also no difference in the TPO activity between hypophysectomized adult females and intact adult ones. These observations show that in foetal rats, TPO activity increases disproportionately to the thyroid weight, but not in newborn and adult rats, and suggest that preferential synthesis of this enzyme occurs in addition to cell hypertrophy during foetal life.

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IRON DEPOSITION IN THE GROWTH PLATE OF LONG BONES OF THE OFFSPRING WHEN GIVEN DURING PREGNANCY IN RAT MODEL

Pakistan Armed Forces Medical Journal ◽

10.51253/pafmj.v71i4.4103 ◽

2021 ◽

Vol 71 (4) ◽

pp. 1223-26

Author(s):

Faiza Umbreen ◽

Khadija Qamar ◽

Sadia Shaukat ◽

Maimoona Khan ◽

Sana Malik ◽

...

Keyword(s):

Growth Plate ◽

Rat Model ◽

Iron Supplementation ◽

Epiphyseal Cartilage ◽

Iron Deposition ◽

Epiphyseal Growth Plate ◽

Adult Rats ◽

Pregnant Rats ◽

Male Adult ◽

Pregnancy And Lactation

Objective: To evaluate histologically the deposition of iron in the epiphyseal cartilage of offspring’s of dams given iron supplementation during pregnancy and lactation in rat model. Study Design: Laboratory based experimental study. Place and Duration of Study: Department of Anatomy, Army Medical College, Rawalpindi and National Institute of Health (NIH) Islamabad, from Mar to Nov 2016. Methodology: In this study, 16 female and 4 male adult rats were chosen for breading. After confirmation of pregnancy, pregnant rats were separated in two groups. One group was given oral iron supplementation for four weeks till delivery and half of the pups fed by mothers who were given iron during lactation. The other group was kept on normal lab diet. Deposition of iron in the epiphyseal cartilage of newborn rats after four weeks was evaluated histologically in pups. Results: Iron deposition was maximum in group C i.e. 1.30 ± 0.48; in group B it was 0.20 ± 0.44. Statistically significant iron deposition (p<0.001) was observed in the growth plate of off springs when mothers were given iron supplements during pregnancy and lactation. Conclusion: Present study proves that injudicious iron supplementation through pregnancy results in deposition of iron in epiphyseal growth plate of the fetus and it can have damaging effects on bones of fetus.

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Involvement of 5-Hydroxytryptamine in Platelet Aggregation In Vivo in Rats and Guinea Pigs

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646615 ◽

1989 ◽

Vol 61 (03) ◽

pp. 463-467 ◽

Cited By ~ 3

Author(s):

G M Smith

Keyword(s):

Platelet Count ◽

Guinea Pigs ◽

Platelet Adhesion ◽

Adenosine Diphosphate ◽

Rate Of Return ◽

Low Doses ◽

Dose Dependent ◽

Higher Doses ◽

Rat Platelets

SummaryIn this study, 5-hydroxytryptamine (5-HT) caused a dose- dependent fall in the circulating platelet count suggesting that 5-HT receptors are activated in rat platelets to cause platelet adhesion and aggregation. When low doses of adenosine diphosphate (ADP) were simultaneously injected with 5-HT, there was a significant potentiation of the responses to ADR Ketanserin significantly reduced the potentiated responses. When higher doses of ADP were infused with bolus injections of 5-HT there was no potentiation and ketanserin did not reduce these responses. Ketanserin did not inhibit the collagen-induced fall in circulating platelet count, but did significantly increase the rate of return to the basal platelet count compared with control. 5-HT did not cause a fall in platelet count in guinea-pigs

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Outcome of Treatment of Deep-Vein Thrombosis with Urokinase: Relationship to Dosage, Duration of Therapy, Age of the Thrombus and Laboratory Changes

Thrombosis and Haemostasis ◽

10.1055/s-0038-1661066 ◽

1984 ◽

Vol 51 (02) ◽

pp. 236-239 ◽

Cited By ~ 28

Author(s):

A D’Angelo ◽

P M Mannucci

Keyword(s):

Degradation Products ◽

High Rate ◽

Bleeding Complications ◽

Vein Thrombosis ◽

Treatment Regimens ◽

Duration Of Therapy ◽

Laboratory Changes ◽

Deep Vein ◽

Dose Dependent ◽

Higher Doses

SummaryForty-one patients with phlebographically proven DVT of the popliteal, femoral or iliac veins were treated with different regimens of urokinase (UK) given by continuous intravenous infusion. The four groups were comparable with respect to localization, extension and estimated age of the thrombi. Another phlebographic picture was taken within 48 hr after the end of UK infusion. Substantial lysis had occurred in 2 of 10 patients treated with 1500 U/kg/h for 2 days, in 4 of 11 treated with 2500/U/kg/h for 3 days, in 2 of 10 treated with 2500 U/kg/h for 7 days and in 4 of 10 treated with 4000 U/kg/h for 4 days. Only thrombi younger than 8 days could be lysed, with 61% (8/13) rate of lysis for thrombi less than 5 days old. Bleeding complications were observed more frequently with the higher doses and longer durations of therapy. The four treatment regimens all induced dose-dependent changes in fibrinogen, fibrin(ogen) degradation products, plasminogen and antiplasmin. Neither pre- nor postinfusion values of these parameters could differentiate patients with lysis from those without lysis. It is concluded that UK can provoke a high rate of thrombolysis of DVT treated early after the appearance of symptoms but that there is no relationship between UK-induced modifications of fibrinolysis and the outcome of therapy.

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Haemato-Clinical Changes in Pregnant and Non-Pregnant Rats, Rattus rattus Linnaeus, 1758 under Parasitic Stress

Egyptian Journal of Veterinary Sciences ◽

10.21608/ejvs.2013.326 ◽

2013 ◽

Vol 44 (1) ◽

pp. 1-19

Keyword(s):

Rattus Rattus ◽

Pregnant Rats ◽

Clinical Changes

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Effect of prenatal isoxsuprine on pulmonary oxygen toxicity in the newborn rat

Journal of Applied Physiology ◽

10.1152/jappl.1980.48.3.505 ◽

1980 ◽

Vol 48 (3) ◽

pp. 505-510 ◽

Cited By ~ 1

Author(s):

L. Frank ◽

J. Summerville ◽

D. Massaro

Keyword(s):

Enzyme Activities ◽

Oxygen Toxicity ◽

Fetal Lung ◽

Antioxidant Enzyme Activities ◽

Newborn Rats ◽

Lung Maturation ◽

Lung Weight ◽

Pregnant Rats ◽

Rat Pups ◽

Hyperoxic Exposure

Isoxsuprine, a beta-sympathomimetic agent used clinically to delay premature parturition and to possibly accelerate fetal lung maturation, was administered to pregnant rats at 48 and 24 h prior to delivery. Newborn rats were placed in 96-98% O2 (or room air) to determine if the prenatal isoxsuprine treatment compromised their tolerance to prolonged hyperoxic exposure. (Exogenous catecholamines are known to exacerbate O2 toxicity in adult animals). Survival of the isoxsuprine-treated pups in O2 (52%) was no different than for control neonates exposed to hyperoxia for 7 days (57%) (P = 0.22). Body weight, lung weight, lung protein, and DNA content of the newborns were also not altered by the prenatal isoxsuprine treatment. Lung antioxidant enzyme activities for superoxide dismutase, catalase, and glutathione peroxidase were the same at birth in the isoxsuprine-treated and control rat pups, and the enzyme activities increased in response to hyperoxic exposure in each group to an equivalent degree. Thus, in utero treatment with isoxsuprine had no apparent adverse effect on newborn rats exposed to a prolonged O2 challenge.

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Prenatal exposure to di-n-butyl phthalate induces erectile dysfunction in male adult rats

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2021.112323 ◽

2021 ◽

Vol 219 ◽

pp. 112323

Author(s):

Xiang Zhou ◽

Tongtong Zhang ◽

Lebin Song ◽

Yichun Wang ◽

Qijie Zhang ◽

...

Keyword(s):

Erectile Dysfunction ◽

Prenatal Exposure ◽

Adult Rats ◽

Male Adult ◽

Butyl Phthalate

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Biological Effect of Different Spinach Extracts in Comparison with the Individual Components of the Phytocomplex

Foods ◽

10.3390/foods10020382 ◽

2021 ◽

Vol 10 (2) ◽

pp. 382

Author(s):

Laura Arru ◽

Francesca Mussi ◽

Luca Forti ◽

Annamaria Buschini

Keyword(s):

Antioxidant Defense ◽

Extraction Methods ◽

Oxidizing Agent ◽

Endogenous Reactive Oxygen Species ◽

Dependent Activity ◽

Main Components ◽

The Individual ◽

Dose Dependent ◽

Higher Doses ◽

Oxidative Insult

The Mediterranean-style diet is rich in fruit and vegetables and has a great impact on the prevention of major chronic diseases, such as cardiovascular diseases and cancer. In this work we investigated the ability of spinach extracts obtained by different extraction methods and of the single main components of the phytocomplex, alone or mixed, to modulate proliferation, antioxidant defense, and genotoxicity of HT29 human colorectal cells. Spinach extracts show dose-dependent activity, increasing the level of intracellular endogenous reactive oxygen species (ROS) when tested at higher doses. In the presence of oxidative stress, the activity is related to the oxidizing agent involved (H2O2 or menadione) and by the extraction method. The single components of the phytocomplex, alone or mixed, do not alter the intracellular endogenous level of ROS but again, in the presence of an oxidative insult, the modulation of antioxidant defense depends on the oxidizing agent used. The application of the phytocomplex extracts seem to be more effective than the application of the single phytocomplex components.

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