scholarly journals Using in silico RFLP method for the study of MC1R gene alleles in the species Sus Scrofa

2021 ◽  
Author(s):  
Victor Sitnic ◽  

Abstract. In the present study we aimed to use a bioinformatics algorithm that predict RFLP fragmentation of swine MC1R alleles simulating the sequence digestion with over 700 restriction enzymes. The results show several restriction enzymes that have the potential to be used for genotyping of Sus scrofa individuals and for differentiation between hybrids and pure line wild boars (WB)/domestic pigs (DP). Genotyping studies of wild boars and domestic pigs in Moldova populations would allow the determination of introgression/backcrossing degree and would contribute to the elaboration of adequate conservation measures

2013 ◽  
Vol 110 (1) ◽  
pp. 104-115 ◽  
Author(s):  
Alain C. Frantz ◽  
Frank E. Zachos ◽  
Julia Kirschning ◽  
Sandra Cellina ◽  
Sabine Bertouille ◽  
...  

1999 ◽  
Vol 73 (1) ◽  
pp. 87-89 ◽  
Author(s):  
E. Pozio ◽  
C.M.O. Kapel

Of 17 Trichinella isolates from domestic pigs and wild boars (Sus scrofa) in regions where Trichinella nativa is widespread among sylvatic animals, two wild boars from Estonia were found to be naturally infected with this Trichinella species. The other 15 animals were infected with Trichinella spiralis. Trichinella nativa is tolerant to freezing when in the muscles of carnivores. The biological characteristics and temperature tolerance of this species in swine need to be further investigated if pork is certified for consumption following freezing.


2012 ◽  
Vol 183 (3-4) ◽  
pp. 393-396 ◽  
Author(s):  
Z. Széll ◽  
G. Marucci ◽  
A. Ludovisi ◽  
M.A. Gómez-Morales ◽  
T. Sréter ◽  
...  

2016 ◽  
Vol 20 (6) ◽  
pp. 756-761 ◽  
Author(s):  
R. B. Aitnazarov ◽  
N. S. Yudin ◽  
R. S. Kiril’chuk ◽  
N. N. Kochnev ◽  
S. P. Knyazev ◽  
...  

2008 ◽  
Vol 54 (4) ◽  
pp. 549-555 ◽  
Author(s):  
Francisco Ruiz-Fons ◽  
Dolo Vidal ◽  
Joaquín Vicente ◽  
Pelayo Acevedo ◽  
Isabel G. Fernández-de-Mera ◽  
...  

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2123
Author(s):  
Anna Koseniuk ◽  
Grzegorz Smołucha ◽  
Małgorzata Natonek-Wiśniewska ◽  
Anna Radko ◽  
Dominika Rubiś

This preliminary study aimed to differentiate domestic pigs from wild boars based on MC1R and NR6A1 polymorphisms and to identify admixture between these genomes. We studied samples obtained from wild boars from two regions of Poland and five pig breeds: Polish Landrace, Polish Large White, Złotnicka White, Pulawska and Duroc. Along the MC1R gene sequence, we identified four polymorphic loci comprising three codons. The “wild type” allele was primarily found in wild boar but also in the Duroc and Złotnicka White breeds. Non-wild type alleles were identified in the vast majority of domestic pig samples and in two wild boar samples. Based on MC1R profiles, we conducted a population study, and revealed admixture between both genomes using STRUCTURE and NETWORK Software. Interestingly, an allelic discrimination assay with NR6A1 g.748C > T TaqMan probes revealed a clear separation of samples into two groups: wild boar samples representing the C allele and domestic breeds representing the T allele. Based on the obtained results, we conclude that NR6A1 g.748C > T is an effective marker for differentiating between wild boars and domestic pigs, where this is supported by MC1R data, to identify admixed profiles. We recommend that a larger sample of genomes is studied to verify this method.


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