scholarly journals Antioxidant Activity of Aspergillus fumigatus

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Daljit Singh Arora ◽  
Priyanka Chandra
Keyword(s):  
Antioxidant Activity ◽  
Aspergillus Fumigatus ◽  
Incubation Temperature ◽  
Reducing Power ◽  
Nitrogen Sources ◽  
Total Phenolic ◽  
Frap Assay ◽  
Carbon And Nitrogen ◽  
Phenolic Contents ◽  
Scavenging Effect

The antioxidant activity of Aspergillus fumigatus was assayed by different procedures and correlated with its extracellular total phenolic contents. Different physio-chemical parameters were optimized to enhance the activity. The culture grown under stationary conditions for 10 days at 25°C at pH 7 gave the best antioxidant activity. Statistical approaches demonstrated sucrose and NaNO3 to be the most suitable carbon and nitrogen sources, respectively. Response surface analysis showed 5% sucrose, 0.05% NaNO3, and incubation temperature of 35°C to be the optimal conditions for best expression of antioxidant activity. Under these conditions, the antioxidant potential assayed through different procedures was 89.8%, 70.1%, and 70.2% scavenging effect for DPPH radical, ferrous ion and nitric oxide ion, respectively. The reducing power showed an absorbance of 1.0 and FRAP assay revealed the activity of 60.5%. Extracellular total phenolic content and antioxidant activity as assayed by different procedures positively correlated.

scholarly journals Phenolic Contents and Antioxidant Potential ofCrataegusFruits Grown in Tunisia as Determined by DPPH, FRAP, andβ-Carotene/Linoleic Acid Assay

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Farouk Mraihi ◽  
Mohamed Journi ◽  
Jamila Kalthoum Chérif ◽  
Munevver Sokmen ◽  
Atalay Sokmen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Frap Assay ◽  
Phenolic Contents ◽  
Dpph Method ◽  
Total Anthocyanins ◽  
Linoleic Acid Assay

Crataegusfruit is one of most important fruits in Tunisian flora. Some fruits of this genus are edible. This study was undertaken in order to examine the benefits of these fruits in human health and their composition of antioxidants including total polyphenol, flavonoids, proanthocyanidins content, and total anthocyanins. The antioxidative properties of the ultrasonic methanolic extract were assessed by different in vitro methods such as the FRAP, DPPH, andβ-carotene/linoleic acid assay. We concluded that peel fraction of red fruits possessed relatively high antioxidant activity and might be a rich source of natural antioxidants in comparison with the pulp and seed fruit extract. The results also showed that hawthorn yellow fruit presents lower amounts of phenolic content, absence of anthocyanins, and less antioxidant capacity. Most of peel and seed fractions were stronger than the pulp fractions in antioxidant activity based on their DPPH IC50, FRAP values, and results ofβ-carotene/linoleic acid. The total phenolic compounds contents were also highly correlated with the DPPH method and the FRAP assay.

scholarly journals Antioxidant Activity of Aqueous Extracts of Different Caffeine Products

2011 ◽  
Vol 39 (2) ◽  
pp. 117 ◽  
Author(s):  
Abd El-Moneim M.R. AFIFY ◽  
Emad A. SHALABY ◽  
Hossam Saad EL-BELTAGI
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
Hot Water ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Power Method ◽  
Tea Leaves ◽  
Total Polyphenol ◽  
Water Extracts ◽  
Phenolic Contents

The antioxidant activity of water extracts (cold and hot) of six caffeine products were carried out. The extracts were screened for total polyphenol contents and antioxidant activity using DPPH, ABTS methods and reducing power method at 50 and 100 μg/ml after 15 min and 30 min using DPPH, ABTS BHA and Caffeine as standard compounds. The results indicated that, the hot water extracts for different caffeine products showed higher antioxidant activity than those of cold extracts and this activity was time and concentration dependent. In addition, the activity was higher against ABTS radical more than DPPH and reducing power methods. Also, there is a positive correlation between the antioxidant and reducing compounds presented in water extracts of different caffeine products. The results of HPLC showed that fresh tea leaves are rich in flavanol monomers known as catechins. The most abundant catechin derivatives in green tea are EGC, EGCG and GC. On the other hand EGCG and GC are major catechin derivative in different caffeine product except El-Fakher tea and Cacao. Generally, these beverages had high antioxidant capacities and total phenolic contents, and could be important dietary sources of antioxidant phenolic for prevention of diseases caused by oxidative stress.

scholarly journals Effect of Extraction Solvent/Technique on the Antioxidant Activity of Selected Medicinal Plant Extracts

Molecules ◽  
2009 ◽  
Vol 14 (6) ◽  
pp. 2167-2180 ◽  
Author(s):  
Bushra Sultana ◽  
Farooq Anwar ◽  
Muhammad Ashraf
Keyword(s):  
Antioxidant Activity ◽  
Organic Solvents ◽  
Reducing Power ◽  
Absolute Methanol ◽  
Terminalia Arjuna ◽  
Total Phenolic ◽  
Plant Materials ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Medicinal Plant Extracts

Theeffects of four extracting solvents [absolute ethanol, absolute methanol, aqueous ethanol (ethanol: water, 80:20 v/v) and aqueous methanol (methanol: water, 80:20 v/v)] and two extraction techniques (shaking and reflux) on the antioxidant activity of extracts of barks of Azadirachta indica, Acacia nilotica, Eugenia jambolana, Terminalia arjuna, leaves and roots of Moringa oleifera, fruit of Ficus religiosa,and leaves of Aloe barbadensis were investigated. The tested plant materials contained appreciable amounts of total phenolic contents (0.31-16.5 g GAE /100g DW), total flavonoid (2.63-8.66 g CE/100g DW); reducing power at 10 mg/mL extract concentration (1.36-2.91), DPPH. scavenging capacity (37.2-86.6%), and percent inhibition of linoleic acid (66.0-90.6%). Generally higher extract yields, phenolic contents and plant material antioxidant activity were obtained using aqueous organic solvents, as compared to the respective absolute organic solvents. Although higher extract yields were obtained by the refluxing extraction technique, in general higher amounts of total phenolic contents and better antioxidant activity were found in the extracts prepared using a shaker.

scholarly journals Phenolic Compounds and Antioxidant Activity of Castanopsis phuthoensis and Castanopsis grandicicatricata

2016 ◽  
Vol 55 ◽  
pp. 77-87 ◽  
Author(s):  
Phung Thi Tuyen ◽  
Do Tan Khang ◽  
Luong The Minh ◽  
Truong Ngoc Minh ◽  
Pham Thi Thu Ha ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Phenolic Extracts

In this study, total phenolic, flavonoid contents, antioxidant capacity, and phenolic compositions of Castanopsis phuthoensis and Castanopsis grandicicatricata (Fagaceae family) were investigated. It was found that bark extracts were rich of phenolic contents, whereas leaf extracts were abundant of flavonoids. The total phenolics varied from 11.20 to 35.47 mg gallic acid equivalent g-1 dry weight (DW), and the total flavonoids were from 2.24 to 12.55 mg rutin equivalent g-1 DW. The results of antioxidant activity showed that the DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activity of the free phenolic extracts were higher than the bound phenolic extracts. Regarding the reducing power and β-carotene bleaching assays, the free phenolic extracts showed remarkably strong antioxidant capacity that were similar to the levels of the standard BHT (dibutyl hydroxytoluene) did. It could be concluded that free phenolic extracts were more effective in antioxidant activities than bound phenolic extracts. A highly significant correlation between phenolic contents and antioxidant activity in extracts were observed. By HPLC analysis, seven phenolic acids were detected including gallic, p-hydroxybenzoic, vanillic, sinapic, p-coumaric, ellagic, and vanillin. Of which, gallic, ellagic, and sinapic acids were the most abundant compounds in the two species. The results suggest C. phuthoensis and C. grandicicatricata contain rich sources of natural antioxidants and phenolic compounds which are probably considered in pharmaceutical use.

scholarly journals Comparative Studies of Phytochemicals Analysis and in vitro Antioxidant Properties of Various Solvent Extracts of Sphaeranthus indicus Collected from three Different Climatic Zones of Central India

2021 ◽  
Vol 33 (9) ◽  
pp. 2237-2243
Author(s):  
Pooja N. Akhand ◽  
Veena Sharma ◽  
Anupam K. Pathak
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Central India ◽  
Total Phenolic ◽  
Climatic Zones ◽  
Phenolic Contents ◽  
Total Antioxidant ◽  
Sphaeranthus Indicus

In present study, the total phenolic, flavonoid, tannin contents and the antioxidant activity of various solvent extracts of Sphaeranthus indicus collected from three different regions of central India were assessed. Plants extracts were prepared using Soxhlation method, while the total phenols, flavonoids and tannins were measured by the spectrophotometric method. DPPH, metal chelating, nitric oxide, superoxide oxide scavenging activity and FRAP, reducing power, total antioxidant assays were also evaluated. The highest phenolic contents 268.22 GAE equivalent, flavonoids 441.33 QE equivalents and tannin content was 120.32 tannic acid equivalents (mg/g) obtained from ethanolic extracts of sample SIEE-1 compared to other two plants extract. Similarly, the highest flavonoid contents was observed in SIAE-1 aqueous extract and lowest in SIAE-2 and SIAE-3. Extract of SIEE-1 possessed maximum antioxidant potentiality and SIAE-2 shown the least antioxidant activity in all assays. It could be concluded that different agroclimatic conditions have effects on the total phenolics, flavonoids, tannin contents and antioxidant potentiality of S. indicus plant.

Antioxidant and Phenolic Characterization with HPLC of Various Extract of Verbascum glomeratum Linneus

2020 ◽  
Vol 42 (2) ◽  
pp. 222-222
Author(s):  
i dem Aydin i dem Aydin ◽  
Akgul Rakhimzhanova Akgul Rakhimzhanova ◽  
zge Kilin arslan and Ramazan Mammadov zge Kilin arslan and Ramazan Mammadov
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Phenolic Constituents ◽  
Ethanol Extracts ◽  
Β Carotene

Verbascum L. species have been used since ancient times in traditional medicine thanks to their bioactive compounds. In this study, we evaluated the antioxidant activity and phenolic contents of ethanolic and acetonic extract of Verbascum glomeratum that collected from Denizli, Turkey. The antioxidant activity were evaluated by using various methods (β-carotene/linoleic acid, ferric reducing power assays (FRAP), radical scavenging assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2and#39;-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS), phoshomolibdenum methods) and total phenolic, flavonoid and tannin contents in the extracts were determined. Antioxidant activity was determined as β-carotene/linoleic acid (%58.5and#177;0.021), DPPH (IC50: 1.220and#177;0.025) and ABTS (IC50:1.552and#177;0.021), ferric reducing antioxidant power (FRAP) (4.151and#177;0.056 mgTE/g), phoshomolibdenum assay (8.218and#177;0.015 mgAE/g) total phenolic, total flavonoid and tannin contents was found as (4.41and#177;0.007 mgGAE/mL), (87.142and#177;0.009 and#181;QE/g), and (30.178and#177;0.008 mgCEs/g) respectively, and then 14 different phenolic compounds in ethanol extracts was carried out by HPLC. Epicatechin (2742.09 and#181;g/g) and 2,5dihidroksi (2544.96 and#181;g/g) were the most abundant phenolic constituents in the extracts. According the results, the extract of V. glomeratum may be considered as a potential source of biological agents.

scholarly journals Effect of the Addition of Propolis Extract on Bioactive Compounds and Antioxidant Activity of Craft Beer

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Pablo A. Ulloa ◽  
Juan Vidal ◽  
María I. Ávila ◽  
Mariela Labbe ◽  
Seth Cohen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Linear Increase ◽  
Total Phenolic ◽  
Frap Assay ◽  
Propolis Extract

Antioxidant-rich foods and beverages play an essential role in the prevention of diseases. This study assessed the influence of the addition of ethanolic extract of propolis (EEP) to beer at different concentrations (0.05, 0.15, and 0.25 g/L). Total phenolic content (TPC) and total flavonoid content (TFC) were determined. Antioxidant activity (AA) was evaluated by radical scavenging activity (DPPH and ABTS) and reducing power (FRAP). The addition of EEP in beer resulted in a linear increase in the TPC with values of 4.5%, 16.7%, and 26.7% above a control (no EEP added; 242 mg gallic acid equivalent/L). A similar increase was observed with TFC values 16.0%, 49.7%, and 59.2% above the control (16.9 mg quercetin equivalent/L). The FRAP assay indicated linear increases in AA relative to control with values of 1555, 1705, and 1892 μmol Trolox equivalent/L following EEP additions. The incorporation of EEP resulted in increases in the bioactive compounds and AA in beer without altering the physicochemical parameters of golden ale beer. The results indicate a promising use of propolis extract as a functional ingredient in beer.

scholarly journals Antioxidant Activities and Estimation of Phenol and Flavonoid Contents in The Extracts Of Trema Orientalis Linn Blume

2021 ◽  
Vol 41 (2) ◽  
pp. 73-84
Author(s):  
A. Saleh ◽  
S.M. Zainal-Ariffin ◽  
S.F. Yahaya ◽  
A.G. Khaleel
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phenolic Contents ◽  
Scavenging Effect ◽  
Inflammatory Conditions ◽  
Trema Orientalis

Phytochemical analysis is important in the evaluation of bioactive compounds from plants. Oxidative stress has been implicated in the pathology of many diseases such as atherosclerosis, rheumatoid arthritis, cancer, cataract, diabetes, cardiovascular diseases, chronic inflammatory conditions, and stroke. The aim of this study was to evaluate polyphenolic content and antioxidant activity of Trema orientalis. Antioxidant activity was estimated spectrophotometrically using 2,2– diphenyl–1–picrylhydrazyl radical scavenging method. The total polyphenolic and flavonoid contents of the Trema orientalis extracts were determined using standard methods. IndependentSample T–test was used for Data analyses. Phytochemical screening revealed the presence of saponins, tannins, steroids, cardiac  glycosides, alkaloids, triterpenes, flavonoids and phenolic compounds. Total phenolic contents were found to be 260.96±2.31 mg GAE/g and 134.08±0.56 mg GAE/g in the ethanol and aqueous extracts respectively. Similarly, total flavonoid contents were between 32.71±0.89 and 4.70±0.23 mg GAE/g. The radical scavenging effect was observed in ethanol extract with IC50 = 9.27 µg/mL. The  abundance of polyphenolic compounds and antioxidant activities of the T. orientalis could confirm their good therapeutic potentials inethnobotany. Keywords: Trema orientalis, Phenols, Flavonoids, Antioxidant activities

scholarly journals Green Synthesis of Silver Nanoparticles from Salvia Aethiopis L. and their Antioxidant Activity

2021 ◽  
Author(s):  
Esma Nur Gecer
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Water Extract ◽  
Reducing Power ◽  
Vibration Signal ◽  
Sem Analysis ◽  
Face Centered Cubic ◽  
Frap Assay ◽  
Scavenging Effect ◽  
Average Size

Abstract Salvia aethiopis L. was heated in distilled water for 2 hours. After filtration, water extract was treated with silver nitrate for 2 hours at 60°C to yield the silver nanoparticles (Sa-AgNPs). The structure of silver nanoparticles was elucidated by spectroscopic methods such as Ultraviolet-visible (UV-Vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and Scanning electron microscope (SEM). The maximum absorption in UV-Vis spectrum was observed at 508 nm. XRD pattern at (2θ) 38.1°, 44.3°, 64.4°, and 77.4° degrees can be assigned to the (111), (200), (220) and (311) Bragg’s reflections of the face-centered cubic crystalline structure. The average size of Sa-AgNPs was found as 74.09 nm by SEM analysis. The characteristic hydroxyl vibration signal appeared at 3222 cm− 1. Antioxidant activity of extract and Sa-AgNPs were carried out using DPPH•, ABTS•+ FRAP assay. The Sa-AgNPs revealed the considerable ABTS•+ scavenging effect with the value of 4.93 (IC50, µg/mL) compared to BHT (IC50, µg/mL, 8.34). However, Sa-AgNPs displayed the lower DPPH• activity (IC50, µg/mL, 24.37) than that of the standard BHT (IC50, µg/mL, 9.67). The reducing power activity of Sa-AgNPs was found as 4.52 (µmol TE/mg extract) while the standard BHT value was 488 (µmol TE/mg extract).

scholarly journals Antioxidant and antiproliferative activities of the n-butanol extract of Centaurea maroccana Ball aerial parts

2021 ◽  
Vol 34 (1) ◽  
pp. 5-11
Author(s):  
Imen Aissous ◽  
Mouad Benrebai ◽  
Ercan Cacan ◽  
Berkel Caglar ◽  
Ramazan Erenler ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Proliferative Activity ◽  
Homo Sapiens ◽  
Quantitative Estimation ◽  
Reducing Power ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Butanolic Extract

Abstract The aim of the present study is to evaluate the total phenolic contents, antioxidant and anti-proliferative activities of the n-BuOH extract of Centaurea maroccana (BECM). The total phenolic and flavonoid of the butanolic extract of the plant were assessed by using Folin-Ciocalteau and aluminium chloride colorimetric assays, respectively, and the quantitative estimation of total flavonoids and phenols revealed the richness of the extract in these compounds. Antioxidant activity was evaluated using standard lab colorimetric methods, while the anti-proliferative activity was evaluated using sulforhodamine B (SRB) assay against C6 (Rattus norvegicus brain glioma) and HT29 (Homo sapiens colorectal adenocarcinoma) cell lines. The n-butanolic extract of Centaurea maroccana showed a strong antioxidant activity through DPPH, ABTS•+, DMSO alkalin, Reducing power, β-Carotene-linoleic acid and CUPRAC assays. Furthermore, the anti-proliferative activity against C6 and HT29 of Centaurea maroccana exhibited a high effect by the decrease in viability of both cancer cell lines. Our results suggest a possible use of Centaurea maroccana as a source of natural antioxidant and chemo-preventive agents against cancer.

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