Antioxidant and Phenolic Characterization with HPLC of Various Extract of Verbascum glomeratum Linneus

2020 ◽  
Vol 42 (2) ◽  
pp. 222-222
Author(s):  
i dem Aydin i dem Aydin ◽  
Akgul Rakhimzhanova Akgul Rakhimzhanova ◽  
zge Kilin arslan and Ramazan Mammadov zge Kilin arslan and Ramazan Mammadov
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Phenolic Constituents ◽  
Ethanol Extracts ◽  
Β Carotene

Verbascum L. species have been used since ancient times in traditional medicine thanks to their bioactive compounds. In this study, we evaluated the antioxidant activity and phenolic contents of ethanolic and acetonic extract of Verbascum glomeratum that collected from Denizli, Turkey. The antioxidant activity were evaluated by using various methods (β-carotene/linoleic acid, ferric reducing power assays (FRAP), radical scavenging assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2and#39;-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS), phoshomolibdenum methods) and total phenolic, flavonoid and tannin contents in the extracts were determined. Antioxidant activity was determined as β-carotene/linoleic acid (%58.5and#177;0.021), DPPH (IC50: 1.220and#177;0.025) and ABTS (IC50:1.552and#177;0.021), ferric reducing antioxidant power (FRAP) (4.151and#177;0.056 mgTE/g), phoshomolibdenum assay (8.218and#177;0.015 mgAE/g) total phenolic, total flavonoid and tannin contents was found as (4.41and#177;0.007 mgGAE/mL), (87.142and#177;0.009 and#181;QE/g), and (30.178and#177;0.008 mgCEs/g) respectively, and then 14 different phenolic compounds in ethanol extracts was carried out by HPLC. Epicatechin (2742.09 and#181;g/g) and 2,5dihidroksi (2544.96 and#181;g/g) were the most abundant phenolic constituents in the extracts. According the results, the extract of V. glomeratum may be considered as a potential source of biological agents.

Antioxidant Activities and Phenolic Composition of Apple Peel, Core and Flesh Extracts on Selected Apple Cultivars

2012 ◽  
Vol 554-556 ◽  
pp. 1103-1109 ◽  
Author(s):  
Gui Zhi Zhang ◽  
Bao Ping Ji ◽  
Gang Chen
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phenolic Antioxidants ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Apple Cultivars ◽  
Apple Peels

Phenolic antioxidants have multiple benefits to human health. Polyphenols are responsible for the antioxidant activity in apples. Antioxidant activities were assessed using the ferric reducing/antioxidant power (FRAP) and 2,2-dipheny l-1-picrylhydrazyl (DPPH) radical scavenging assays in different cultivars and different parts of apples grown in China. The phenolic compounds and antioxidant activities differed significantly among the four apple cultivars. Guoguang had the highest phenolic concentration and antioxidant activity and Golden Delicious had the lowest. The peels had the highest total phenolic and flavonoid contents followed by the cores and flesh. Anthocyanins were detected only in red apple peels. Peel and core had greater antioxidant activities than apple flesh. FRAP values were inversely correlated with phenolic contents, whereas no clear relationship could be observed between DPPH values and phenolic contents. The higher levels of phenolic compounds and antioxidant activities of apple peels and cores than flesh may be of technological interest as a valuable source of antioxidants.

scholarly journals Enhancing Phenolic Contents and Antioxidant Potentials ofAntidesma thwaitesianumby Supercritical Carbon Dioxide Extraction

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Warut Poontawee ◽  
Surapol Natakankitkul ◽  
Orawan Wongmekiat
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Added Value ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Phenolic Constituents ◽  
Supercritical Carbon

Supercritical fluid extraction (SFE) has increasingly gained attention as an alternative technique for extraction of natural products without leaving toxic residues in extracts.Antidesma thwaitesianumMuell. Arg. (Phyllanthaceae), or ma mao, has been reported to exhibit antioxidant health benefits due to its phenolic constituents. To determine whether SFE technique could impact on phenolic contents and associated antioxidant potentials, ripe fruits ofAntidesma thwaitesianum(Phyllanthaceae) were extracted using supercritical carbon dioxide (SC-CO2) and conventional solvents (ethanol, water). The results showed that the SC-CO2extract contained significantly higher yield, total phenolic, flavonoid, and proanthocyanidin contents than those obtained from ethanol and water. It also demonstrated the greatest antioxidant activities as assessed by ABTS radical cation decolorization, DPPH radical scavenging, and ferric reducing antioxidant power (FRAP) assays. Further analysis using high-performance liquid chromatography with diode array and mass spectrometry detectors (HPLC-DAD/MSD) revealed the presence of catechin as a major phenolic compound ofAntidesma thwaitesianum(Phyllanthaceae), with the maximum amount detected in the SC-CO2extract. These data indicate that SFE technology improves both quantity and quality ofAntidesma thwaitesianumfruit extract. The findings added more reliability of using this technique to produce high added value products from this medicinal plant.

scholarly journals In vitro Bioactivity of Essential Oils and Methanol Extracts of Salvia reuterana from Iran

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700
Author(s):  
Javad Safaei Ghomi ◽  
Reihaneh Masoomi ◽  
Fereshteh Jookar Kashi ◽  
Hossein Batooli
Keyword(s):  
Linoleic Acid ◽  
Essential Oils ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Methanol Extracts ◽  
Phenolic Contents ◽  
Β Carotene

The chemical composition of the essential oils, antioxidant activity (DPPH and β-carotene/linoleic acid assays) and total phenolic content (Foline-Ciocalteu) of the flowers and leaves of Salvia reuterana were determined. Essential oils extracted from the flowers and leaves by hydrodistillation were analyzed by GC and GC/MS. Forty-four constituents, representing 99.7-99.9% of the oils, were identified. The major components were germacrene D, benzoic acid hexyl ester, bicyclogermacrene, β-gurjunene and ishwarene, constituting 33.7-31.9% of the oils. The highest radical-scavenging activity (DPPH test) was shown by the methanol extract of the flowers (IC50=77.6 μg/mL). In the β-carotene/linoleic acid assay, the methanol extract of the leaves showed the highest inhibition (40.3%) which was only slightly lower than that shown by BHT (82.9%). The total phenolic contents of the methanol extracts of the flowers and leaves as gallic acid equivalents were 81.4 and 88.3 μg/mg, respectively. The plant also showed good antimicrobial activity against three strains of tested microorganisms.

scholarly journals Phenolic Compounds and Antioxidant Activity of Castanopsis phuthoensis and Castanopsis grandicicatricata

2016 ◽  
Vol 55 ◽  
pp. 77-87 ◽  
Author(s):  
Phung Thi Tuyen ◽  
Do Tan Khang ◽  
Luong The Minh ◽  
Truong Ngoc Minh ◽  
Pham Thi Thu Ha ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Phenolic Extracts

In this study, total phenolic, flavonoid contents, antioxidant capacity, and phenolic compositions of Castanopsis phuthoensis and Castanopsis grandicicatricata (Fagaceae family) were investigated. It was found that bark extracts were rich of phenolic contents, whereas leaf extracts were abundant of flavonoids. The total phenolics varied from 11.20 to 35.47 mg gallic acid equivalent g-1 dry weight (DW), and the total flavonoids were from 2.24 to 12.55 mg rutin equivalent g-1 DW. The results of antioxidant activity showed that the DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activity of the free phenolic extracts were higher than the bound phenolic extracts. Regarding the reducing power and β-carotene bleaching assays, the free phenolic extracts showed remarkably strong antioxidant capacity that were similar to the levels of the standard BHT (dibutyl hydroxytoluene) did. It could be concluded that free phenolic extracts were more effective in antioxidant activities than bound phenolic extracts. A highly significant correlation between phenolic contents and antioxidant activity in extracts were observed. By HPLC analysis, seven phenolic acids were detected including gallic, p-hydroxybenzoic, vanillic, sinapic, p-coumaric, ellagic, and vanillin. Of which, gallic, ellagic, and sinapic acids were the most abundant compounds in the two species. The results suggest C. phuthoensis and C. grandicicatricata contain rich sources of natural antioxidants and phenolic compounds which are probably considered in pharmaceutical use.

scholarly journals Influence of Conventional Fermentation on Antioxidant Activity and Phenolic Contents of Two Common Dairy Products: Yogurt and Kefir

2020 ◽  
Vol 8 (6) ◽  
pp. 1277-1282
Author(s):  
Bilge Taşkın ◽  
Neriman Bağdatlıoğlu
Keyword(s):  
Phenolic Content ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Chelating Ability ◽  
Natural Foods ◽  
Dpph Radical Scavenging

During conventional fermentation of milk into yogurt and kefir, changes of antioxidant capacities (DPPH radical scavenging, ferrous chelating, reducing power, hydrogen peroxide/ H2O2 scavenging activity) and total phenolic content were investigated. Both products showed greater phenolic content, DPPH radical scavenging, and reducing power activities than was the case of their unfermented milk forms. But they were unable to scavenge H2O2. Chelating ability of milk increased by yogurt fermentation but decreased by kefir fermentation. In general, antioxidative capacity of milk improved throughout conventional yogurt and kefir fermentations and ripening periods (except chelating ability for kefir and H2O2 scavenging). This study is valuable since it evaluates the antioxidant power of yogurt and kefir produced by traditional fermentation with 5 different antioxidative approaches. In face of rapidly rising consumer demand for safe, healthy, functional but natural foods, revealed results might strengthen importance of conventional fermentation process and highlight the antioxidative contributions of mentioned products

Disparities of phytochemical constituents and antioxidant activities of some Indigofera species

2021 ◽  
pp. 1-10
Author(s):  
Firdose R. Kolar ◽  
Sidanand V. Kambhar ◽  
Manjula Chavan ◽  
Shruti Kadam ◽  
Peerambi Nadaf
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Plant Material ◽  
Pearson Correlation ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Phytochemical Constituents

Abstract The current study was intended to examine the difference in the phytochemical and antioxidant profile of nine Indigofera species. With this aim, the species were assessed for total phenolic content, flavonoid content, and antioxidant activity using ferric reducing antioxidant power (FRAP), DPPH free radical scavenging, ferrous ion chelating activity, phosphomolybdenum reducing power, deoxyribose degradation, β-carotene bleaching, nitric oxide scavenging, and superoxide radical scavenging assays. The content of total phenolics and flavonoids ranged from 1.33 to 22.5 mg TAE/g plant material and 0.47 to 5.02 mg QE/g plant material respectively in the various species, while the antioxidant activity as tested with different antioxidant test models varied with the species studied as well as with the solvents used for the extraction. Interestingly, all the tested extracts demonstrated considerable free radical scavenging activity, moreover, I. hirsuta, I. glandulosa, I. linnaei, I. hochstetteri, I. linifolia, and I. trita were found to be the most effective among the species studied. The results indicated the significant differences in phytochemical constituents and the antioxidant activity among the species. The Pearson correlation coefficient analysis exhibited a significant correlation of phenolics and flavonoids with the antioxidant activity.

In vitro antioxidant and enzyme inhibitory properties and phenolic contents of crude extracts and fractions from different organs of the halophyte Lycium europaeum L.

2021 ◽  
Vol 17 ◽  
Author(s):  
Houaria Bendjedou ◽  
Malika Bennaceur ◽  
Houari Benamar ◽  
Maria João Rodrigues ◽  
Catarina Pereira ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biological Properties ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Ic50 Values ◽  
Reducing Activity ◽  
Ethanol Extracts ◽  
High Level ◽  
Β Carotene

Background: Lycium europaeum L. is a medicinal and edible Mediterranean halophyte spiny shrub. However, studies regarding its biological properties focused mainly on its aerial organs. Objective: The objective of the present work was to make a comparative evaluation of the in vitro antioxidant and inhibitory enzyme activities of ethanol extracts and fractions (chloroform, ethyl acetate, n-butanol and aqueous) from roots and leaves of L. europaeum, along with its total phenolic, flavonoid and tannin contents. Methods: The antioxidant activity was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), superoxide radical, β-carotene bleaching, cupric reducing, and the ferric reducing activity methods. Results: The n-butanol fraction from roots had the highest antioxidant activity in all the assays. It was also the most active against acetylcholinesterase, butyrylcholinesterase, and urease (IC50 values 92.63, 118.26, and 135.60 µgmL-1, respectively). This fraction showed a high level of total phenolic, flavonoid, and tannin contents. Conclusion: The results suggest L. europaeum, especially its roots, as a candidate to be further explored as a source of bioactive products.

scholarly journals Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity

2015 ◽  
Vol 67 (3) ◽  
pp. 829-840 ◽  
Author(s):  
Nemanja Stanisavljevic ◽  
Marija Ilic ◽  
Zivko Jovanovic ◽  
Tihomir Cupic ◽  
Dragana Dabic ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phenolic Composition ◽  
Phenolic Contents ◽  
Metal Chelating ◽  
Phenolic Constituents ◽  
Seed Coats

The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu?s method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antioxidant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products.

scholarly journals Anti-Inflammatory and Antioxidant Activities ofSalvia fruticosa: An HPLC Determination of Phenolic Contents

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Rima Boukhary ◽  
Karim Raafat ◽  
Asser I. Ghoneim ◽  
Maha Aboul-Ela ◽  
Abdalla El-Lakany
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Aerial Parts ◽  
Phenolic Constituents ◽  
Anti Inflammatory

Objectives.Salvia fruticosaMill. (S. fruticosa) is widely used in folk medicine. Accordingly, the present study was designed to evaluate the antioxidant and anti-inflammatory activities ofS. fruticosa, and to determine the phenolic constituents of its extracts.Methods. The antioxidant activity was determined using 2,2-diphenylpicrylhydrazyl assay. Total phenolic contents were estimated using Folin-Ciocalteu reagent, and high-performance liquid chromatography was performed to identify phenolic constituents. To evaluate the anti-inflammatory activity, carrageenan-induced mouse paw edema was determined plethysmographically.Key Findings. Different plant extracts demonstrated strong radical scavenging activity, where the ethyl acetate extract had the highest value in the roots and the lowest in the aerial parts. This antioxidant activity was correlated to the total phenolic content of different extracts, where rutin and luteolin were the most abundant constituents. Interestingly, both the roots and aerial parts revealed a significant anti-inflammatory activity comparable to diclofenac.Conclusions. This study is the first to demonstrate pharmacologic evidence of the potential anti-inflammatory activity ofS. fruticosa. This activity may partly be due to the radical scavenging effects of its polyphenolic contents. These findings warrant the popular use of the East Mediterranean sage and highlight the potential of its active constituents in the development of new anti-inflammatory drugs.

scholarly journals Bioactive Compounds and Biochemical and Antioxidant Properties of Selected Minor Indigenous Fruits in Bangladesh

2021 ◽  
Vol 11 (1) ◽  
pp. 35-46
Author(s):  
Molla M.M ◽  
Sabuz A.A ◽  
Chowdhury M.G.F ◽  
Khan M.H.H ◽  
Alam M ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Array Detector ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Antioxidant Power

Minor fruits are a potential source of antinutrients, but there is no complete primary data source in the Bangladeshi context. Therefore, the present study was undertaken to acquire documentation for a database of the composition of selected minor fruits. The total phenolic (TPH), vitamin C, total carotene, and ß-carotene contents and antioxidant activity of selected minor fruits were determined by 1,1-diphenyl-2picryl hydrazyl (DPPH) scavenging and reducing power assays (RPA). Phenolic compounds were assessed using high-performance liquid chromatography coupled with a photodiode array detector and autosampler. Results revealed that minor fruits contain different phytochemicals, particularly TPH, ascorbic acid, total flavonoid (TF), ß-carotene, total carotenoid (TC), and total anthocyanin content (TAC); values ranged, respectively, 0.23-176.50 mg GAE/g, 16.67-664.92 mg/100 g, 2.26-150.02 mg QE/100 g, 1.41-6897.57 µg/100 g, 1.26-98.24 mg/100 g and 1.15-47.46 mg/100 g. In the parameters antioxidant activity, total antioxidant capacity, DPPH, reducing power capacity (RPC), ferric reducing antioxidant power (FRAP), metal chelating capacity (MCC), nitric oxide (NO), and free radical scavenging activity, IC50 ranged 0.01-278.24 µg of ascorbic acid/mg of extract, 39.70-250.00%, 3.21-634.00%, 0.02-1817.88 µM Fe2SO4/100g, 22.29-210.43%, 0.02-70.50%, and 4.98-856.70 µg/g, respectively. Among the identified and quantified phenolic acids, leading examples were gallic acid (279.06 mg/100 g), vanilic acid (43.77 mg/100 g), Þ-courmaric acid (178.96 mg/100 g), ferulic acid (20.44 mg/100 g), and lutein (91.13 µg/100 g) in aonla, day fruit, elephant apple, and bilimbi. Moreover, all selected minor fruits are rich sources of bioactive, biochemical, and antioxidant compounds with potential for use in therapeutic applications.

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