scholarly journals Detection and Typing of Human Papilloma Virus by Multiplex PCR with Type-Specific Primers

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Francisco Romero-Pastrana
Keyword(s):  
High Risk ◽  
Human Papilloma Virus ◽  
Multiplex Pcr ◽  
Agarose Gel Electrophoresis ◽  
Specific Primers ◽  
Papilloma Virus ◽  
Hpv Typing ◽  
Attractive Option ◽  
Hpv Types ◽  
High Risk Hpv

The primary underlying cause of cervical cancer is infection with one or more high-risk (HR) types of the human papilloma virus (HPV). Detection and typing of HPV have been commonly carried out by PCR-based assays, where HPV detection and typing are two separate procedures. Here, we present a multiplex PCR-based HPV typing assay that detects 20 HPV types (15 HR, 3 probably HR and 2 low risk) using type-specific primers and agarose gel electrophoresis. 46 cervical, urethral, and biopsy samples were analyzed by both Multiplex PCR and PGMY09/11 consensus PCR, and results were compared. 611 samples were further analyzed by Multiplex PCR, 282 were positive for HR HPV, and 101 showed multiple HR HPV infections. The relatively ease and economic accessibility of the method and its improved ability to detect high-risk HPV types in multiple HPV-infected samples make it an attractive option for HPV testing.

scholarly journals Results of the first human papilloma virus center in Hungary (2007–2011)

2011 ◽  
Vol 152 (45) ◽  
pp. 1804-1807
Author(s):  
Ádám Galamb ◽  
Attila Pajor ◽  
Zoltán Langmár ◽  
Gábor Sobel
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papilloma Virus ◽  
Hpv Testing ◽  
Papilloma Virus ◽  
Cytological Atypia ◽  
Hpv Dna ◽  
International Data ◽  
Hpv Types ◽  
High Risk Hpv

Human papilloma virus (HPV) is the most common sexually transmitted infection in the 21st century. It has been established that infections with specific HPV types are contributing factors to cervical cancer. Approximately 99.7% of cervical cancers are associated with high risk HPV types. HPV testing plays an important role in the prevention, by decreasing the prevalence and the mortality of cervical cancer. There are 16 HPV-centers operating in Hungary, in which patients undergo HPV screening, cervical exams, and treatment based on standardized guidelines. Patients and methods: The first HPV-center was founded in 2007 in Budapest, at the 2nd Department of Obstetrics and Gynecology, Semmelweis University. This study aimed to define the presence and prevalence of HPV-DNA in the cervical swab samples obtained from patients in our center. Authors conducted to assess the age-specific-prevalence, and HPV type distribution, the associated cervical abnormalities, comparing our results with international data. Results: Overall 1155 woman underwent HPV-testing and genotyping, using polymerase chain reaction. Overall, 55.5% of patients had positive test for HPV DNA types, in which 38.5% for high-risk HPV DNA. Overall prevalence was the highest among females aged 15 to 25years (62.9%). The most common HPV type found was the high risk type 16 (19.5% among the patients with positive HPV testing). Presence of high risk HPV with concurrent cervical cytological abnormality was in 32%. More than two-thirds of woman with cytological atypia (70.6%) were infected with two or more high risk HPV types. HPV 16 was detected in 32% of patients with cytological abnormalities. Conclusions: The results suggest that the prevalence of HPV in this study population exceeds the international data. The results attracts the attention the peak prevalence of the high risk types in the youngest age-group, and the higher risk of cervical abnormality in case of presence of two or more HPV types. The dominance of type 16 and 18 was predictable, but the strong attendance of type 51 and 31 among patients who had cytological atypia, was slightly surprising. Orv. Hetil., 2011, 152, 1804–1807.

scholarly journals Genotyping of High-risk Human Papilloma virus (HPV) among Iraqi women in Baghdad by Multiplex PCR

2015 ◽  
Vol 9 (1) ◽  
pp. 38-45
Author(s):  
Ashna J. Faik Faik ◽  
Mudhafar Q. Saber Saber ◽  
Wisam J. Mohammed Mohammed ◽  
Bashar Z. Ibraheem Ibraheem ◽  
Kawther R. Lateef Lateef ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papilloma Virus ◽  
Multiplex Pcr ◽  
Dna Amplification ◽  
Hpv Infection ◽  
Abnormal Cytology ◽  
Normal Cytology ◽  
Papilloma Virus ◽  
Hpv Types

Human papilloma virus (HPV) infection is a causative factor for cervical cancer. Early detectionrisk HPV types might help to identify women at high risk of cervical cancer. The aim of of highHPV infection in population of rgir hgih determine the occurrence of the present study was toIraqi women in Baghdad by using Multiplex PCR determine the percentage and genotyping ofHuman Papilloma Virus and to put the best prevention and control program in Iraqi women.Study started at January 2009 to March 2010, cervical samples were collected from 856 womenaged 16–70. HPV DNA amplification was performed using HPV High Risk Typing PCR Kit testfor qualitative detection and genotyping of HPV types 16, 18, 31, 33, 35, 39, 45, 52, 56, 58, 59, 66 inHPV was detected in 106 ( 12,38% ) of the study population, with a range of the cervical swabs.16-70 years age groups. Results showed that the overall HPV prevalence twelve genotypes wereidentified, including HPV-33 (18.60%), HPV-35 (18.60%), HPV-56 (18.60%) ,HPV-39(10.85%),HPV-52 (10.08%), HPV-18 (7.75%), HPV-16 (4.65%), HPV-59 (4.65%), HPV-58(2.32%), HPV-31(1.55%), HPV-45(1.55% ) and HPV-66( 0.77%). Of 856, 218 women was also tested by pap smearith normal cytology was 198 ( 90.83%), 24(12.12%) of them were HPV positive, those with w,abnormal cytology was 20 (9.17 %), 5( 25%)of them was HPV positive. In this study unlike otherepidemiological studies, HPV33,35,56 was the most frequent type (55.8%) in Baghdad, followedby HPV39, HPV52, HPV18, HPV16.

Quantitative multiplex PCR assay for the detection of the seven clinically most relevant high-risk HPV types

2009 ◽  
Vol 44 (4) ◽  
pp. 302-307 ◽  
Author(s):  
Martina Schmitz ◽  
Cornelia Scheungraber ◽  
Jörg Herrmann ◽  
Karin Teller ◽  
Mieczyslaw Gajda ◽  
...  
Keyword(s):  
High Risk ◽  
Multiplex Pcr ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Hpv Types ◽  
High Risk Hpv

scholarly journals Human Papilloma Virus Types 16/18 Distribution in Invasive Cervical Cancer: An Evidence for Vaccination in Bihar, India

2021 ◽  
pp. 59-68
Author(s):  
Rahul Kumar ◽  
Vinita Trivedi ◽  
Richa Chauhan ◽  
Akhtar Parwez ◽  
Biplab Pal ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papilloma Virus ◽  
Vaccination Program ◽  
Hpv 16 ◽  
Tissue Samples ◽  
Papilloma Virus ◽  
Cancer Tissues ◽  
High Risk Hpv ◽  
Hpv 18

There is high incidence of cervical cancer in Bihar, India. Vaccination for cervical cancer in developed countries has played a crucial role in limiting the incidence rate of cervical cancer worldwide. In consideration of debate on clinical efficacy of Human Papilloma Virus (HPV) vaccine in India, study on the prevalence of high risk HPV 16/18 strains in different regions of the nation becomes very crucial. Few individual states have started vaccination but centralised vaccination program does not exist due to lack of sufficient genotypic study of Human Papilloma Virus in different parts of India. Bihar is the third most populous state of India and HPV 16/18 distribution has not been reported yet. The nationwide data of HPV 16/18will help to develop a unified centralised vaccination program. We carried out a distribution study of high risk HPV type 16 and 18 in cervical cancer patients attending a tertiary care hospital of Bihar, India.HPV 16/18 types were analysed in cervical cancer tissues (n = 96) of patients attending the regional cancer hospital of Bihar. Tissue samples were analysed for HPV 16 and HPV 18 using a Real Time PCR technique. The results suggest very high prevalence of HPV 16/18. HPV was identified in all the samples (96/96). About, 74 (77.08%) samples presented with HPV 16 whereas, 16 (16.67%) of the samples presented with HPV 18. 6 Co-infection was presented in 6 (6.25%) of the samples of cervical cancer tissues. HPV 16/18 prevalence is more in the women aged between 41 to 61 years.We report 100% prevalence of HPV16/18 in the cervical cancer tissue samples. A way to minimise this gynaecological concern would be to introduce prophylactic vaccines and early screening in the state of Bihar. The data generated would be crucial in drafting for community screening of HPV. We strongly emphasize the prophylactic HPV Vaccination against HPV 16 to control the alarming rate of cervical cancer in one of the most populous state of India, Bihar.

scholarly journals Distinction Between Vaginal and Cervical Microbiota in High-risk Human Papilloma Virus Infected Women in China

2020 ◽  
Author(s):  
Zhan Zhang ◽  
Ting Li ◽  
Dai Zhang ◽  
Xiaonan Zong ◽  
Huihui Bai ◽  
...  
Keyword(s):  
High Risk ◽  
Human Papilloma Virus ◽  
Chinese Women ◽  
Variable Region ◽  
Vaginal Microbiota ◽  
Rrna Gene ◽  
Papilloma Virus ◽  
Next Generation Sequencing Technology ◽  
History Of ◽  
High Risk Hpv

Abstract Background: High-risk human papilloma virus (hrHPV) is regarded as the main causal factor of cervical precancer and cancer when persistent infection is left untreated. Previous studies have declared that HPV is associated with microecological environment of lower genital tract but haven’t discriminate vaginal microbiota from that of cervix. Objective: To analyze the distinction between vaginal and cervical microbiota in high-risk HPV(+) Chinese women. Methods: One hundred participants were recruited including 20 healthy women with HPV (-), 32 with other hrHPV (+), 38 subjects with HPV16/18 (+) and 10 with cervical carcinoma, demographics of whom were collected and analyzed. Vaginal and cervical microbiota were separately tested through next-generation sequencing technology (NGS) targeting the variable region (V3-V4) of bacterial ribosome 16S rRNA gene. Results: 1. Analysis of demographics demonstrated that hrHPV infected women tend to be accustomed to vaginal douching (p =0.001), show more frequent usage of sanitary pads (p =0.007), have more sex partners (p =0.047), be more sexually active (p =0.025), have more diverse ways of contraception (p =0.001) and history of vaginitis (p =0.002). 2. NGS identified microbial diversity of cervical microbiota was much higher than that of vagina with significantly increased Proteobacteria and decreased Lactobacillus. Variation of cervical microbiota of hrHPV(+) subjects partly similar to vaginal microbiota but had its unique features. Sphingomonas of α-Proteobacteria, almost invisible in the vagina, was more frequent at normal cervix whereas decreased remarkably at hrHPV(+) cervix. Reversely, γ-Proteobacteria showed a significant positive correlation with HPV16/18 and cervical cancer. BV related anaerobes like Gardnerella, Prevotella, Atopobium and Sneathia showed similar changes in both vaginal and cervical microbiota of hrHPV(+) women and did not exhibit cervical specificity. Conclusions: Cervical microbiota has its uniqueness from that of vagina in bacterial communities presenting a higher proportion of Proteobacteria, of which Sphingomonas is potentially predictive of a health guardian of hrHPV while Pseudomonas the opposite.

scholarly journals Clinico-epidemiological profile of condylomata acuminata with special emphasis on HPV typing

2017 ◽  
Vol 3 (3) ◽  
pp. 332
Author(s):  
A. Sarin ◽  
V. G. Binesh ◽  
Betsy Ambooken ◽  
S. Suprakasan
Keyword(s):  
High Risk ◽  
Condylomata Acuminata ◽  
Transmitted Infection ◽  
Hpv 16 ◽  
Sexually Transmitted ◽  
Hbsag Positivity ◽  
Hpv Typing ◽  
Hpv Types ◽  
Epidemiological Profile ◽  
High Risk Hpv

<p class="abstract"><strong>Background:</strong> <span lang="EN-IN">Condylomata acuminata (CA) or genital wart is a sexually transmitted infection (STI) caused by human papilloma virus (HPV). As there was little information about the HPV types commonly causing CA in our state, we decided to study the clinico-epidemiological profile of CA with special emphasis on HPV typing.</span></p><p class="abstract"><strong>Methods:</strong> We did <span lang="EN-IN">HPV typing in 25 clinically diagnosed cases of CA. The biopsied specimens were sent for HPV typing using Polymerase chain reaction (PCR) and those with high risk HPV types were histologically analyzed for any dysplastic change. We also evaluated for other STIs which can coexist with CA</span>.<strong></strong></p><p class="abstract"><strong>Results:</strong> <span lang="EN-IN">Out of the 25 cases, 18 (72%) were males and 7 (28%) females. Majority of our patients belonged to 21-40 year age group (64%). Clinical types of CA were the classical fleshy exuberant type in 22 patients (88%), flat type in 2 (8%) and keratotic type in 1 (4%). HPV positivity was obtained in 21 (84%). The most common type was HPV 6 in 10 cases (40%) and HPV 11 in 8 (32%) patients. HPV 16 alone was isolated in 1 (4%) case. HPV types 6 and 16 and 11 &amp; 18 were isolated in one case each. Dysplastic changes were observed in two cases with HPV 16 and 18. VDRL and TPHA positivity was found in 2 (8%) males and HBsAg positivity was seen in 1 (4%) male patient. </span></p><p class="abstract"><strong>Conclusions:</strong> <span lang="EN-IN">This study highlights the importance of HPV typing for identifying the high risk types and to assess the efficacy of HPV vaccines.</span></p>

scholarly journals Prevalence of High-Risk Human Papillomavirus Types Among Women Screened for Cervical Cancer in Yazd, Iran, and Comparison of Cytology, Histology, and Colposcopy Results

2020 ◽  
Vol 13 (9) ◽  
Author(s):  
Mojgan Karimi-Zarchi ◽  
Nastaran Hajimaghsoudi ◽  
Afsarosadat Tabatabai ◽  
Mansour Moghimi ◽  
Mohammad Shayestehpour ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
High Risk ◽  
Pap Smear ◽  
Blot Hybridization ◽  
High Grade ◽  
Cervical Lesions ◽  
Hpv Typing ◽  
Hpv Types ◽  
High Risk Hpv

Background: Human Papillomavirus (HPV) is a DNA virus with more than 100 genotypes, at least 12 of which are high-risk and associated with high-grade cervical lesions. Data on the prevalence of high-risk HPV genotypes among women are not yet available for the total regions of Iran. Objectives: The present study aimed to determine the prevalence of high-risk HPV types among women screened for cervical carcinoma in Yazd and compare the cytology, histology, and colposcopy results. Methods: In this cross-sectional study, 402 women referring to gynecology clinics of Shahid Sadoughi University of Medical Sciences, Yazd, Iran, were selected. The Pap smear and HPV typing were performed on cervical samples. The high-risk HPV types were detected by the polymerase chain reaction (PCR)-based reverse blot hybridization assay. Colposcopy was carried out on patients with high-risk HPV types, and biopsies were taken for histological examination. Results: Among 402 women screened by HPV-PCR, 32 (7.97%) women were positive for high-risk HPV types. Human papillomavirus 16 and HPV18 were the most frequent genotypes (46.9%). The cytology, histology, and colposcopy results were abnormal in 56.2%, 29.1%, and 71.9% of patients, respectively. Pap smear had 100% sensitivity and 58.3% specificity for the detection of high-grade cervical lesions, while these values for colposcopy were 75% and 87.5%, respectively. Conclusions: The frequency of high-risk HPV types was relatively low among women living in Yazd than in those from other provinces of Iran. A significant percentage of patients with HPV had normal cervical cytology and histology. Therefore, HPV typing is recommended to decrease the development of cervical cancer. Colposcopy had acceptable sensitivity and specificity for the detection of high-grade cervical lesions.

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