Comparative Proteomic Analysis for a Putative Pyridoxal Phosphate-Dependent Aminotransferase Required for Virulence in Acidovorax citrulli

Acidovorax citrulli (Ac) is the causative agent of bacterial fruit blotch disease in watermelon. Since resistant cultivars have not yet been developed, the virulence factors/mechanisms of Ac need to be characterized. This study reports the functions of a putative pyridoxal phosphate-dependent aminotransferase (PpdaAc) that transfers amino groups to its substrates and uses pyridoxal phosphate as a coenzyme. It was observed that a ppdaAc knockout mutant had a significantly reduced virulence in watermelon when introduced via germinated-seed inoculation as well as leaf infiltration. Comparative proteomic analysis predicted the cellular mechanisms related to PpdaAc. Apart from causing virulence, the PpdaAc may have significant roles in energy production, cell membrane, motility, chemotaxis, post-translational modifications, and iron-related mechanisms. Therefore, it is postulated that PpdaAc may possess pleiotropic effects. These results provide new insights into the functions of a previously unidentified PpdaAc in Ac.

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Comparative Proteomic Analysis of Histone Post-translational Modifications upon Ischemia/Reperfusion-Induced Retinal Injury

Journal of Proteome Research ◽

10.1021/pr500040a ◽

2014 ◽

Vol 13 (4) ◽

pp. 2175-2186 ◽

Cited By ~ 10

Author(s):

Xiaolu Zhao ◽

Simone Sidoli ◽

Leilei Wang ◽

Wenjun Wang ◽

Lin Guo ◽

...

Keyword(s):

Proteomic Analysis ◽

Ischemia Reperfusion ◽

Post Translational Modifications ◽

Comparative Proteomic Analysis ◽

Retinal Injury

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Comparative proteomic analysis reveals insights into the dynamic responses of maize (Zea mays L.) to Setosphaeria turcica infection

Plant Science ◽

10.1016/j.plantsci.2020.110811 ◽

2021 ◽

pp. 110811

Author(s):

Yuwei Liu ◽

Xiaodong Gong ◽

Qihui Zhou ◽

Yajie Liu ◽

Zhenpan Liu ◽

...

Keyword(s):

Zea Mays ◽

Proteomic Analysis ◽

Zea Mays L ◽

Dynamic Responses ◽

Setosphaeria Turcica ◽

Comparative Proteomic Analysis

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Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

International Journal of Molecular Sciences ◽

10.3390/ijms22126323 ◽

2021 ◽

Vol 22 (12) ◽

pp. 6323

Author(s):

Alexander L. Rusanov ◽

Peter M. Kozhin ◽

Olga V. Tikhonova ◽

Victor G. Zgoda ◽

Dmitry S. Loginov ◽

...

Keyword(s):

Proteomic Analysis ◽

Living Organism ◽

Peritoneal Macrophages ◽

In Vitro Models ◽

Individual Characteristics ◽

Essential Proteins ◽

Comparative Proteomic Analysis ◽

Immortalized Cell ◽

The Individual

In vitro models are often used for studying macrophage functions, including the process of phagocytosis. The application of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization and higher variability of the obtained results. Immortalized cell lines do not have these disadvantages, but their responses to various signals can differ from those of the living organism. In the present study, a comparative proteomic analysis of immortalized PMJ2-R cell line and primary peritoneal macrophages isolated from C57BL/6 mice was performed. A total of 4005 proteins were identified, of which 797 were quantified. Obtained results indicate significant differences in the abundances of many proteins, including essential proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, and Msr1. These findings indicate that outcomes of studies utilizing PMJ2-R cells as a model of peritoneal macrophages should be carefully validated. All MS data are deposited in ProteomeXchange with the identifier PXD022133.

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