scholarly journals Efficient N-Dimensional surface estimation using Crofton formula and run-length encoding

2012 ◽  
Author(s):  
Gaetan Lehmann ◽  
David Legland

Unlike the measure of the area in 2D or of the volume in 3D, the perimeter and the surface are not easily measurable in a discretized image. In this article we describe a method based on the Crofton formula to measure those two parameters in a discritized image. The accuracy of the method is discussed and tested on several known objects. An algorithm based on the run-length encoding of binary objects is presented and compared to other approaches. An implementation is provided and integrated in the LabelObject/LabelMap framework contributed earlier by the authors.

2003 ◽  
Vol 125 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Joseph L. Hammack ◽  
Diane M. Henderson

Experiments are conducted in a three-dimensional wave basin with a wavemaker system comprising 32 side-by-side paddles for which there is precise control. Two types of wavemaker forcings are used to create two-dimensional surface patterns: (1) two symmetric carrier waves interacting at an oblique angle and (2) a single carrier wave propagating in the x-direction with a Jacobi elliptic, sn-function modulation in the y-direction. Data are presented from overhead photographs and from time series obtained by traversing a wave-gage through the patterns. Two parameters are systematically varied: the horizontal aspect ratio of the cells comprising the surface pattern and the measure of nonlinearity of the input wavefield. Unlike such waves in shallow water for which the surface pattern is made up of six-sided cells, the wave pattern for waves in deep water is made up of rectangular cells. Both the overhead photographs and the time series show that for most values of the two parameters, the wavefields evolve with significant modulations in both the x and y directions. In particular, when the aspect ratio of the cells is below about 0.4 for a fixed measure of nonlinearity, there is significant modulation in the y-direction that results in cells with smaller aspect ratios. For aspect ratio above about 0.4, the cells appear to be stable (except for viscous decay) for smaller values of nonlinearity. However, for larger values of nonlinearity even these cells modulate in the y-direction, further increasing the aspect ratio of the evolving cells. For the largest value of nonlinearity considered, the pattern evolves into one that comprises cells with aspect ratios of about 1.


Author(s):  
Joseph L. Hammack ◽  
Diane M. Henderson

Experiments are conducted in a three-dimensional wave basin with a wavemaker system comprising 32 side-by-side paddles for which there is precise control. Two types of wavemaker forcings are used to create two-dimensional surface patterns: (1) two symmetric carrier waves interacting at an oblique angle and (2) a single carrier wave propagating in the x-direction with a Jacobi elliptic, sn-function modulation in the y-direction. Data are presented from overhead photographs and from time series obtained by traversing a wave-gage through the patterns. Two parameters are systematically varied: the horizontal aspect ratio of the cells comprising the surface pattern and the measure of nonlinearity of the input wavefield. Unlike such waves in shallow water for which the surface pattern is made up of six-sided cells, the wave pattern for waves in deep water is made up of rectangular cells. Both the overhead photographs and the time series show that for most values of the two parameters, the wavefields evolve with significant modulations in both the x and y directions. In particular, when the aspect ratio of the cells is below about 0.4 for a fixed measure of nonlinearity, there is significant modulation in the y-direction that results in cells with smaller aspect ratios. For aspect ratio above about 0.4, the cells appear to be stable (except for viscous decay) for smaller values of nonlinearity. However, for larger values of nonlinearity even these cells modulate in the y-direction, further increasing the aspect ratio of the evolving cells. For the largest value of nonlinearity considered, the pattern evolves into one that comprises cells with aspect ratios of about 1.


1966 ◽  
Vol 24 ◽  
pp. 170-180
Author(s):  
D. L. Crawford

Early in the 1950's Strömgren (1, 2, 3, 4, 5) introduced medium to narrow-band interference filter photometry at the McDonald Observatory. He used six interference filters to obtain two parameters of astrophysical interest. These parameters he calledlandc, for line and continuum hydrogen absorption. The first measured empirically the absorption line strength of Hβby means of a filter of half width 35Å centered on Hβand compared to the mean of two filters situated in the continuum near Hβ. The second index measured empirically the Balmer discontinuity by means of a filter situated below the Balmer discontinuity and two above it. He showed that these two indices could accurately predict the spectral type and luminosity of both B stars and A and F stars. He later derived (6) an indexmfrom the same filters. This index was a measure of the relative line blanketing near 4100Å compared to two filters above 4500Å. These three indices confirmed earlier work by many people, including Lindblad and Becker. References to this earlier work and to the systems discussed today can be found in Strömgren's article inBasic Astronomical Data(7).


Author(s):  
H.A. Cohen ◽  
W. Chiu

The goal of imaging the finest detail possible in biological specimens leads to contradictory requirements for the choice of an electron dose. The dose should be as low as possible to minimize object damage, yet as high as possible to optimize image statistics. For specimens that are protected by low temperatures or for which the low resolution associated with negative stain is acceptable, the first condition may be partially relaxed, allowing the use of (for example) 6 to 10 e/Å2. However, this medium dose is marginal for obtaining the contrast transfer function (CTF) of the microscope, which is necessary to allow phase corrections to the image. We have explored two parameters that affect the CTF under medium dose conditions.Figure 1 displays the CTF for carbon (C, row 1) and triafol plus carbon (T+C, row 2). For any column, the images to which the CTF correspond were from a carbon covered hole (C) and the adjacent triafol plus carbon support film (T+C), both recorded on the same micrograph; therefore the imaging parameters of defocus, illumination angle, and electron statistics were identical.


Author(s):  
T. L. Hayes

Biomedical applications of the scanning electron microscope (SEM) have increased in number quite rapidly over the last several years. Studies have been made of cells, whole mount tissue, sectioned tissue, particles, human chromosomes, microorganisms, dental enamel and skeletal material. Many of the advantages of using this instrument for such investigations come from its ability to produce images that are high in information content. Information about the chemical make-up of the specimen, its electrical properties and its three dimensional architecture all may be represented in such images. Since the biological system is distinctive in its chemistry and often spatially scaled to the resolving power of the SEM, these images are particularly useful in biomedical research.In any form of microscopy there are two parameters that together determine the usefulness of the image. One parameter is the size of the volume being studied or resolving power of the instrument and the other is the amount of information about this volume that is displayed in the image. Both parameters are important in describing the performance of a microscope. The light microscope image, for example, is rich in information content (chemical, spatial, living specimen, etc.) but is very limited in resolving power.


Author(s):  
David P. Bazett-Jones ◽  
Mark L. Brown

A multisubunit RNA polymerase enzyme is ultimately responsible for transcription initiation and elongation of RNA, but recognition of the proper start site by the enzyme is regulated by general, temporal and gene-specific trans-factors interacting at promoter and enhancer DNA sequences. To understand the molecular mechanisms which precisely regulate the transcription initiation event, it is crucial to elucidate the structure of the transcription factor/DNA complexes involved. Electron spectroscopic imaging (ESI) provides the opportunity to visualize individual DNA molecules. Enhancement of DNA contrast with ESI is accomplished by imaging with electrons that have interacted with inner shell electrons of phosphorus in the DNA backbone. Phosphorus detection at this intermediately high level of resolution (≈lnm) permits selective imaging of the DNA, to determine whether the protein factors compact, bend or wrap the DNA. Simultaneously, mass analysis and phosphorus content can be measured quantitatively, using adjacent DNA or tobacco mosaic virus (TMV) as mass and phosphorus standards. These two parameters provide stoichiometric information relating the ratios of protein:DNA content.


Author(s):  
J. Zhang ◽  
D.B. Williams ◽  
J.I. Goldstein

Analytical sensitivity and spatial resolution are important and closely related factors in x-ray microanalysis using the AEM. Analytical sensitivity is the ability to distinguish, for a given element under given conditions, between two concentrations that are nearly equal. The analytical sensitivity is directly related to the number of x-ray counts collected and, therefore, to the probe current, specimen thickness and counting time. The spatial resolution in AEM analysis is determined by the probe size and beam broadening in the specimen. A finer probe and a thinner specimen give a higher spatial resolution. However, the resulting lower beam current and smaller X-ray excitation volume degrade analytical sensitivity. A compromise must be made between high spatial resolution and an acceptable analytical sensitivity. In this paper, we show the necessity of evaluating these two parameters in order to determine the low temperature Fe-Ni phase diagram.A Phillips EM400T AEM with an EDAX/TN2000 EDS/MCA system and a VG HB501 FEG STEM with a LINK AN10 EDS/MCA system were used.


Author(s):  
J. Bonevich ◽  
D. Capacci ◽  
G. Pozzi ◽  
K. Harada ◽  
H. Kasai ◽  
...  

The successful observation of superconducting flux lines (fluxons) in thin specimens both in conventional and high Tc superconductors by means of Lorentz and electron holography methods has presented several problems concerning the interpretation of the experimental results. The first approach has been to model the fluxon as a bundle of flux tubes perpendicular to the specimen surface (for which the electron optical phase shift has been found in analytical form) with a magnetic flux distribution given by the London model, which corresponds to a flux line having an infinitely small normal core. In addition to being described by an analytical expression, this model has the advantage that a single parameter, the London penetration depth, completely characterizes the superconducting fluxon. The obtained results have shown that the most relevant features of the experimental data are well interpreted by this model. However, Clem has proposed another more realistic model for the fluxon core that removes the unphysical limitation of the infinitely small normal core and has the advantage of being described by an analytical expression depending on two parameters (the coherence length and the London depth).


1973 ◽  
Vol 29 (02) ◽  
pp. 363-374 ◽  
Author(s):  
F. K Beller ◽  
W Theiss

SummaryPlasma fibrinogen, circulating fibrinmonomers (as indicated by a positive ethanol gelation test), fibrinolysis breakdown products and plasma hemoglobin were assayed in 122 rats subjected to endotoxin injection or infusion. The results were correlated with the quantitative measurement of glomerular fibrin deposition. Based on these data four groups were determined : consumption coagulopathy and three stages of increasing severity of disseminated intravascular coagulation (DIG).Consumption coagulopathy was defined by a decrease in plasma fibrinogen and a positive ethanol gelation test in the absence of glomerular fibrin deposition. Plasma hemoglobin and fibrinolysis breakdown products were normal or only slightly increased.DIG as characterized by glomerular fibrin deposition was defined as moderate (1 to 20% glomeruli showing fibrin strands), intermediate (21 to 80%), and severe (81 to 100%). Decrease in plasma fibrinogen and frequence of a positive ethanol gelation test in all stages of DIG were only slightly different from the findings in consumption coagulopathy. However, a sharp increase in plasma hemoglobin levels was noted when glomerular fibrin deposition did occur even in small amounts. At this time only a moderate increase was noted in fibrin(ogen) breakdown products. These two parameters increased only slightly in the group of intermediate DIG. Severe DIG was characterized by a massive increase in fibrin (ogen) breakdown products and high levels of plasma hemoglobin.


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